Labshake search
Citations for Zymo Research :
701 - 750 of 6307 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The assembly products were then cleaned and eluted in 6 μL water (Zymo Clean and Concentrate). Then ...
-
bioRxiv - Microbiology 2023Quote: ... or placed in 1.5ml cryovials containing 750 ul Xpedition™ RNA/DNA Shield (Zymo Research Products) and stored at ambient temperature before transportation to the laboratory where they were subsequently stored at -20°C until DNA extraction22 ...
-
bioRxiv - Genomics 2024Quote: ... Gibson assembled products were then cleaned and eluted in 10 μL water (Zymo Clean and Concentrate). 1 μL of cleaned product was then added to 25 μL NEB-10β E ...
-
bioRxiv - Cancer Biology 2019Quote: ... RNA was extracted from intestinal cells using TRIzol followed by column purification (Zymo RNA clean and concentrator column) following the manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by pooling and purification of ligated footprints using an Oligo Clean and Concentrator column (Zymo Research, D4060). Ribosomal RNA contamination was removed by biotinylated primers (McGlincy and Ingolia 2017) ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by 2.5 μL of 2M HCl and purification by DNA Clean and Concentrator-5 columns (Zymo Research) using an 8:1 ratio of DNA Binding Buffer to cDNA volume ...
-
bioRxiv - Plant Biology 2024Quote: ... Final DNA purification was performed with the Zymogen Genomic DNA Clean and Concentrator-10 column (Zymo Research, Irvine). Libraries were prepared using SMRTbell Template Prep Kit 1.0 and sequenced on a PacBio Sequel platform at Novogene.
-
bioRxiv - Systems Biology 2022Quote: ... DNA was eluted in 60 μl elution buffer and further purified by using the OneStep PCR Inhibitor Removal Kit (ZymoBIOMICS, Zymo Research, Irvine, CA, USA).
-
bioRxiv - Microbiology 2024Quote: ... The three PCR fragments were purified (Zymo DNA Clean and Concentrator kit ...
-
bioRxiv - Molecular Biology 2023Quote: ... The CIP-treated RNA was subjected to another round of purification using the Direct-zol™ RNA MicroPrep (Zymo) kit ...
-
bioRxiv - Molecular Biology 2020Quote: ... tagmentation products from 200ng HEK293T total RNA were purified by DNA Clean & Concentrator-5 (Zymo Research, Cat.No.D4013) and eluted with 20μl nuclease-free water ...
-
bioRxiv - Immunology 2019Quote: Amplified products of the expected size for ERV-K envelope were extracted from agarose gels (Zymo Research) and ligated into pCR-Blunt II-TOPO vector for sequencing (Thermo Fisher) ...
-
bioRxiv - Immunology 2021Quote: ... followed by a 15 minute treatment with DNase and subsequent purification with Direct-zol™ RNA MiniPrep Plus (Zymo Research). All shRNAs used in this study can be found in Supplementary Dataset 2.
-
bioRxiv - Molecular Biology 2021Quote: ... This library pool was then size selected on a 2% agarose gel by cutting between 150-800 nucleotides and performing gel purification (Zymo). To determine the loading density of the final pooled library ...
-
bioRxiv - Plant Biology 2019Quote: ... Purification of small and large RNAs into separate fractions was carried out using RNA Clean & Concentrator TM −5 (Zymo Research) and concentration and quality of the fractions were checked again ...
-
bioRxiv - Microbiology 2023Quote: ... Nucleic acid extraction and purification were performed using the Quick-DNA/RNA™ Viral MagBead (Zymo Research, Irvine, California, USA) following the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the 100–400nt region was cut and dissolved in 1X TEN250 buffer (1X TE 7.4, 0.25M NaCl) at 80°C for 30min followed by column purification (Zymo research, R1016). For the HEK293T libraries ...
-
bioRxiv - Microbiology 2023Quote: ... The 60 samples were pooled into 5 groups of 12 samples each during purification using RNA Clean & Concentrator (Zymo Research) and all eluted with 16µl DEPC UltraPure water.
-
bioRxiv - Evolutionary Biology 2023Quote: ... for 30 minutes at 37 °C and products were further cleaned with RNA clean & concentrator 5 (Zymo Research) and eluted with 8-10 µl RNase free water ...
-
bioRxiv - Molecular Biology 2022Quote: ... The barcoding PCR reactions were further pooled and gel-extracted using Zymo gel extraction kit and DNA clean & concentrator (Zymo research Cat. #: 11-301 and 11-303) and quantified by Qubit 1x dsDNA HS assay kits (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... triplicate cell samples or 50 uL fresh mouse blood were harvested into Trizol reagent before purification via Direct-zol RNA miniprep (Zymo Research) per manufacturer’s instructions with an additional dry spin after disposing of the final wash to prevent carryover ...
-
bioRxiv - Immunology 2019Quote: ... followed by RNA purification from the aqueous phase using a modified version of the Zymo RNA Clean and Concentrator (Zymo Research), through the addition of 2x volumes of ethanol to increase the retention of small RNA species ...
-
bioRxiv - Microbiology 2021Quote: ... Exceptions to these protocols were as follows: (i) RNA purification after acylation was performed using RNA C&C columns (Zymo Research), rather than ethanol precipitation ...
-
bioRxiv - Cell Biology 2022Quote: ... An aliquot of the supernatant was then used for RNA purification with on-column DNase-I treatment (Direct-Zol RNA Microprep R2060, Zymo Research). RNA concentration and purity was determined by spectrophotometry (NanoDrop 1000 ...
-
bioRxiv - Microbiology 2020Quote: ... were added to media-removed cell monolayers per single well of 12 well plates followed by on-column DNA digestion and purification (Zymo Research). For RNA purification from RNP capture sample ...
-
bioRxiv - Cancer Biology 2020Quote: ... Transposition was carried out at 37°C for 30 minutes followed by DNA purification with DNA Clean and Concentrator-25 (Zymo Research) according to manufacturer’s recommendation ...
-
bioRxiv - Cell Biology 2021Quote: ... human islets or EndoC-βH5 cells using a commercial column-based purification with DNAse I treatment (Direct-Zol RNA MicroPrep with Trizol Reagent, Zymo Research). RNA was quantified by Nanodrop spectrophotometer (ThermoFisher Scientific) ...
-
bioRxiv - Genomics 2022Quote: ... The samples were treated with DNase I and cleaned using ethanol precipitation and subsequent RNA Clean & Concentrator-5 spin-column purification (Zymo Research). Successful removal of genomic DNA was confirmed by PCR using a host-specific primer pair (Supplementary Figure S1) ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was digested by 2M NaOH and the reaction was neutralized with 1M Tris-HCl (pH 7.5) followed by column purification (Zymo research, R1016). The 5’adapter (for C2C12 libraries ...
-
bioRxiv - Bioengineering 2023Quote: ... but modified by inclusion of an additional RNase A treatment and a purification step using Zymo-Spin III columns (Zymo-Research). Purity of gDNA was assessed using either a Denovix DS-11 spectrophotometer or the Qubit 3 fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... A second RNA extraction using TRIZOL was carried out and completed by RNA purification with Direct-zol RNA Miniprep (Zymo Research). RNA quality and quantity were determined using a NanoDrop 2000 (Thermo Scientific) ...
-
bioRxiv - Microbiology 2023Quote: Escherichia coli, Strain 83972 (BEI Resources, Manassas, VA, USA) and two mock community products (Zymo Research, Irvine, CA, USA) were used for validation of the ssUMI pipeline ...
-
bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... 10 μL of 2X ZymoTaq PCR Mix (Zymo Research), PCR primers ...
-
bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
-
bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Equal volumes of fractions 10-16 were pooled and RNA was by acidic phenol chloroform extraction followed by column purification (Zymo Research, R1013) as described above ...
-
bioRxiv - Molecular Biology 2023Quote: TM4 and C-terminal tail point mutations were made by digesting Asu-ACR-16-REM and Dme-ACR-16-REM with Bsu15I and ApaI (ThermoScientific, USA) followed by gel purification (Zymo Research, USA). These were then ligated to oligo inserts containing the TM4 and C-terminal tail point mutations (S Table 2) ...
-
bioRxiv - Zoology 2023Quote: ... cucullus vegetative cells (300,000 cells) and 2-week-old mature cysts (3,000,000 cells) using the Direct-zol RNA purification system (Zymo Research Crop., California, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... 7) We verified the presence of high molecular weight DNA products in the samples before purifying nucleic acids by Zymo DNA Clean & Concentrator-5 (ZYMO Research).
-
bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
-
bioRxiv - Biophysics 2023Quote: ... The reactions were quenched with β-mercaptoethanol (50% by volume) followed by column purification (Oligo Clean & Concentrator, Zymo Research #D4060, here and below). The adenylated 18 nt unique molecular indicator (UMI ...
-
bioRxiv - Genomics 2020Quote: ... The reaction was then treated with DNase I to remove template DNA and RNA product was purified using an RNA Clean and Concentrator-5 (Zymo #R1016). Purified RNA was used as the template for reverse transcription using ProtoScript II according to manufacturer protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
-
bioRxiv - Genetics 2022Quote: ... The resulting RNA product was run on 12% SequaGel -UreaGel (national diagnostics) and purified with ZR small-RNA PAGE Recovery KitTM (R1070, ZYMO RESEARCH) following provider’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... Beads were then washed several times with buffers and RNA purification was performed on eluted beads (Zymo RNA Clean & Concentrator −5, R1013, Zymo Research, Irvine, CA, USA). RNA quality and concentration were determined via Agilent BioAnalyzer prior to sequencing.
-
bioRxiv - Molecular Biology 2021Quote: ... PCR products were run on a 1% agarose gel to check the product size and purified using Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). To create the cloning insert ...
-
bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to a new 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and heated to 98°C for 10 minutes ...