Labshake search
Citations for Zymo Research :
651 - 700 of 821 citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The total RNA and RF samples were purified using a modified RNA Clean & Concentrator-5 kit method (Zymo Research). The volume of each sample was increased to 100 µl ...
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bioRxiv - Genomics 2023Quote: ... cytosines in 87bp DNA fragment were modified with hydroxy-methylation by PCR amplification with 5-hydroxymethyl-dCTP supplement (ZYMO). The hydroxy-methylated DNA fragment was combined with equal amounts of CpG methylated pUC19 DNA (NEB ...
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bioRxiv - Genomics 2023Quote: ... the resulting poly-A+ RNA fraction (corresponding to 0.7-2% of the starting amount) was then purified again on a Clean & Concentrator-5 Column Kit (Zymo) and eluted in 10µL of molecular-grade water.
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bioRxiv - Genomics 2023Quote: ... cleaned up either with phenol-chloroform and precipitated with ethanol or with RNA Clean & Concentrator -5 kit (Zymo Research). Two biological replicates were collected for each genotype ...
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bioRxiv - Molecular Biology 2023Quote: ... cfRNAs were then purified and concentrated into 6 µl using an RNA Clean and Concentrator-5 kit (Zymo, R1016). Plasma extracellular vesicles (EVs ...
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bioRxiv - Neuroscience 2024Quote: ... the RNA samples were purified and concentrated using an RNA Clean & Concentrator-5 Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Then the nucleic-acid bound protein isolation was performed using buffers from the Quick-RNA Miniprep Kit (Zymo Research) and the Zymo-Spin IIICG Column (Zymo Research) ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous RNA cross-linked to bait protein was recovered from the beads using the QuickRNA Microprep Kit (Zymo Research). Complementary DNA was synthesized using the SuperScript III First-Strand Synthesis kit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... DNA was captured on protein A/G agarose beads and purified using the ChIP DNA Clean & Concentrator kit (Zymo) and used for qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... treated for two hrs using 2 units of DNase I per hour and was cleaned of residual DNase I with the RNA clean and concentrator-5 (Zymo). DNase-treated RNA was then divided into two equal aliquots ...
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bioRxiv - Molecular Biology 2021Quote: ... For RNA analysis 5% of input material and 70% of elution were processed by the addition of 400 µl RNA lysis buffer (Zymo) and RNA extraction using the Zymo Quick-RNA™ MicroPrep RNA extraction kit (Zymo ...
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bioRxiv - Molecular Biology 2020Quote: ... The enriched RNA and input total RNA from 5% of lysate were purified using Direct-zol RNA Purification Kit (Zymo). Target genes in input total RNA and enriched RNA were quantified by qRT-PCR using SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) ...
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bioRxiv - Genomics 2019Quote: ... The reaction was incubated 10 minutes at 55°C followed by purification with DNA Clean & Concentrator-5 kit (Zymo Research) and elution in 21 µL of water ...
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bioRxiv - Cancer Biology 2020Quote: ... the transposed DNA fragments were harvested and cleaned up with Zymo DNA Clean and Concentrator-5 kit (D4011, Zymo Research). The libraries were indexed and amplified with Illumina i5/i7 indexing primers (FC-121-1011 ...
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bioRxiv - Plant Biology 2021Quote: ... RNA samples were digested with DNase I and purified using RNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR ...
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bioRxiv - Bioengineering 2021Quote: 1 μg of genomic DNA was hydrolyzed overnight at 37 ºC with 5 U of DNA Degradase Plus (Zymo Research) following the manufacturer’s protocol in 50 μl volume ...
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bioRxiv - Plant Biology 2021Quote: ... Lysis tubes were shaken 5 min at 16 Hz using a ball mill to extract and purify DNA following the manufacturer’s guidelines (Zymo Research). Microbiome profiling of isolated DNA samples was performed by Eurofins Genomics (Ebersberg ...
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bioRxiv - Molecular Biology 2022Quote: ... The assembly is cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 3 μl Gel Loading Dye ...
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bioRxiv - Bioengineering 2022Quote: ... The IVT reaction was assembled based on the manufacturer’s recommendations and the sgRNA was purified using an RNA Clean & Concentrator-5 kit (Zymo Research). RNA concentration was measured using a NanoDrop (ThermoFisher)
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bioRxiv - Plant Biology 2021Quote: ... The RNA was then purified and concentrated using the RNA Clean Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). cDNA libraries were prepared using a mix of 50 ng RNA and 0.5 ng Spike-in RNA Variant Control Mix E2 (Lexogen ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated using the PerfectPure RNA Cultured Cell kit (5 PRIME) or Quick-RNA™ MiniPrep (Zymo Research). One microgram of the total RNA was reverse-transcribed into cDNA with M-MLV (Promega ...
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bioRxiv - Immunology 2021Quote: ... Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo). Fragments were amplified according to the previously published protocol ...
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bioRxiv - Bioengineering 2019Quote: ... the minimal medium was supplemented with 20 μg/ml uracil and 250 μg/ml 5-fluorororic acid (FOA) (Zymo Research) or 60 g/l sucrose (counter-selection with sacB).
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bioRxiv - Molecular Biology 2019Quote: ... per manufacturer protocol and reaction products were purified with the Zymo Clean & Concentrator - 5 kit (Zymo Research #R1016, Irvine CA). RNA concentration post labeling was measured with the Qubit RNA HS kit (ThermoFisher #Q32852) ...
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bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA fragments were purified from the reaction using a RNA Clean & Concentrator(tm)-5 column (Zymo Research, Cat. n° R1013). The resulting RNA fragments were ligated to different adaptors (listed in Supplementary Table 1 ...
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bioRxiv - Plant Biology 2019Quote: ... Purification of small and large RNAs into separate fractions was carried out using RNA Clean & Concentrator TM −5 (Zymo Research) and concentration and quality of the fractions were checked again ...
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bioRxiv - Genetics 2020Quote: ... viral RNA was prepared from 200 µl of clarified virus stocks (3.000 rpm, 5 min) with the Quick-RNA Viral Kit (Zymo research) and eluted in 14 µl RNase free H20 ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from lumbar DRGs 2-5 following the manufacturer’s protocol with a Direct-zol RNA Miniprep Plus kit (Zymo R2071). RNA libraries were prepared and sequenced by the UTSW Microarray core facility on an Illumina NextSeq SE-75 sequencer at 40 million reads/sample ...
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bioRxiv - Genetics 2021Quote: RNA was isolated from eight 5 days old males using ZR Tissue and Insect RNA MicroPrep™ (Zymo Research, #R2030). DNA was removed using TURBO™ DNase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... collect ~5 roots and fully submerge in a pre-labelled 2 mL microcentrifuge tube filled with DNA/RNA Shield (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... RNA was cleaned and concentrated to 10 μl using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA), as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... P7 and E18.5 was directly dissolved in RNA isolation buffer and RNA was isolated using the Quick-RNA micro prep kit (Zymo). Library preparation was done using the NEB Next® Ultra™ RNA Library Prep Kit ...
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bioRxiv - Cell Biology 2022Quote: ... all wells were pooled to purify DNA products using the Zymo DNA Clean & Concentrator-5 kit (Zymo, Cat #: 11-303) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and DNA was extracted at days 1 and 5 utilizing the Quick-DNA/RNA Miniprep (Zymo Research). Viral genomes were quantified using a standard curve and normalized to RNAseP.
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bioRxiv - Microbiology 2023Quote: Total DNA was isolated from ∼6x105-7x105 MRC-5 infected fibroblasts using Zymo Duet RNA/DNA isolation Kit (Zymo Research). Viral genomes were quantitated by quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... PCR purification and plasmid isolation was done using the DNA Clean & Concentrator-5 and Zyppy Plasmid Miniprep Kits from Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted and purified using Trizol reagent and RNA Clean & Concentrator-5 kit (Zymo Research cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
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bioRxiv - Genomics 2023Quote: ... the aqueous phase was transferred to an RNA Clean and Concentrator-5 column and purified according to the manufacturer’s instructions including on-column DNaseI digestion (Zymo Research).
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bioRxiv - Biochemistry 2022Quote: ... decorated cDNA from multiple samples were pooled together and purified using the DNA Clean & concentrator-5 Kit (Zymo research, #D4014). After elution with 20 µl of nuclease free water ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... treated with 1 μL of DpnI for 3 h at 37℃ and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo CN# D4004). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Microbiology 2023Quote: ... The 60 samples were pooled into 5 groups of 12 samples each during purification using RNA Clean & Concentrator (Zymo Research) and all eluted with 16µl DEPC UltraPure water.
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from various developmental stages and adult tissues using TRIzol Reagent (Thermo Fischer Scientific, Waltham, MA, USA) and further purified with the RNA clean and concentrator-5 kit (Zymo), following the provided instructions ...
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bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...