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Citations for Zymo Research :
601 - 650 of 744 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... lysate from ∼1×106 cells in the well of a 6-well plate was collected in 300 µL of DNA/RNA Shield (Zymo). RNA was isolated using the Quick-RNA Miniprep Plus Kit (Zymo ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were prepared and transformed with plasmids using the Mix and Go™ kit (Zymo Research, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were infected with 229E at an MOI of 1.0 and were lysed with RNA lysis buffer (Zymo# R1035) at 1 ...
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bioRxiv - Cell Biology 2023Quote: DNA extraction from whole input cells and purified nucleoli was performed using a Quick-DNA Universal Kit (Zymo Research, CA). DNA concentration was measured using a Qubit dsDNA HS Assay kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were scraped into solution and total RNA was purified using Zymo Quick-RNA Miniprep kit (Zymo Research, R1054). Of the isolated total RNA ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA of the cells transduced with the sgRNA library were extracted with Genomic DNA Clean & Concentrator (Zymo Research D4010). We amplified 100 ng genomic DNA with the same two-round strategy ...
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bioRxiv - Genetics 2024Quote: ... A cell line was prepared encoding this donor plasmid by transforming BL21(DE3) and making the resultant cell line chemically competent using Mix & Go preparation kit (Zymo). The temperature sensitive donor plasmid was then transformed with a second plasmid encoding a T7-inducible recombinase and a constitutively expressed bridge RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from MV4;11 Cas9 + GeCKOv2 cells collected before and following sustained C6-treatment using the Quick-gDNA MidiPrep Kit (Zymo Research) per manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: Sorted cKithighCD11blow AML cells were lysed in Trizol reagent and RNA was extracted using Direct-zol RNA miniprep kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from approximately 105 − 106 cells was extracted from the cultures of the evolution experiments every 48 hours using Quick-DNA miniprep kits (Zymo Research). The HIV RT gene amplified by PCR from the proviral RNA using Phusion hot start II DNA polymerase (New England Bio-labs ...
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Molecular Biology 2021Quote: ... reporter assay was carried out as described above in presence of Nsp1 or Nsp2 followed by harvesting of cells in Trizol (Zymo Research). The RNA was isolated using Direct-zol RNA miniprep kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from the sorted or the control cells either using the Quick-DNA Mini Kit or the Midi Plus Kit (Zymo Research). The integrated LentiCRISPRv2 vector sgRNA sequences were amplified through three rounds of PCR using the Taq DNA Polymerase S (high specificity ...
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bioRxiv - Cell Biology 2022Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
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bioRxiv - Genomics 2020Quote: ... DNA was then extracted from approximately 1 ml of the red blood cell pellets using Zymo Bead Genomic DNA kit (Zymo Research) following the manufacturer’s procedures ...
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bioRxiv - Cell Biology 2019Quote: ... Genotyping PCR was performed using Taq polymerase on genomic DNA extracted from SMB55 cells using ZymoBead™ Genomic DNA kit (Zymo). Genomic DNA extracted from Ptch1-/- mouse embryonic fibroblasts and a wild-type mouse were used as a control for the mutant and wild-type alleles respectively ...
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bioRxiv - Neuroscience 2019Quote: ... The gDNA of the sorted cells (both N2A and NSCs) was extracted with the Genomic DNA(tm) - Tissue MiniPrep kit (Zymo Research) following the protocol of the manufacturer for cell suspensions ...
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bioRxiv - Microbiology 2019Quote: Genomic extraction was preformed on 105 – 106 cells from the in vitro evolution experiments using the Quick-DNA Miniprep Plus Kit (Zymo Research). The gDNA was then diluted to a range between 100-200ng/5μl in TE buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Sorted cells were collected in PBS, centrifuged (2000 rpm, 5 min) and RNA extracted using the Quick RNA Mini Prep (Zymo Research) according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: Total cellular RNA from BMDM cell lines or tissues was isolated using the Direct-zol™ RNA MiniPrep Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA (gDNA) was isolated from a saturated overnight culture (approximately 2 x 108 cells) using YeaStar Genomic DNA Kit (Zymo Research). Genomic DNA (125 µL at 15 ng µL−1 in ultrapure water ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Synthetic Biology 2020Quote: ... yeast strain was used to make competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research).
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bioRxiv - Bioengineering 2019Quote: ... 1.5-mL aliquots of cell culture were used as input in minipreps with the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: The cDNA of all single cells was collected together and further concentrated with the DNA Clean and Concentrator−5 kit (Zymo Research). The Exonuclease I (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: RNA extraction of whole lung pieces or purified cells was performed using the Quick-RNA Miniprep or Quick-RNA Microprep (Zymo Research), respectively ...
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bioRxiv - Cell Biology 2021Quote: mIMCD3 wild type and Jade mutant cells were washed with PBS and RNA extraction was performed with the Direct-zol RNA Miniprep kit (Zymo Research) following the manufacturer’s instructions including a DNase1 treatment step ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from Vero cells after 4 days post infection using the Direct-zol RNA isolation kit (Zymo Research). 1 μg of RNA isolate was subjected to rRNA removal with the NEBNext rRNA Depletion Kit (NEB ...
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bioRxiv - Microbiology 2020Quote: Dim and lit cells were sorted into 500 µl TriZol and RNA was extracted with DirectZol RNA purification kit (Zymo Research) with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Infected Huh-7 cells (with or without medium) or mouse lung tissues were lysed in DNA/RNA shield reagent (Zymo Research) and total RNA was extracted by using RNeasy kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... 5 µL of ligated plasmid was then transformed into competent BW23473 cells (made using the Mix & Go E. coli Transformation Kit & Buffer Set, Zymo Research) and plated on LB + amp plates and grown overnight ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from cultured cells using BIO TRI RNA reagent (Bio-lab) and Direct-zol RNA MiniPrep kit (Zymo research), as instructed by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... The cells and medium were then collected and subjected to RNA extraction using the Direct-zol RNA MiniPrep kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA from either CD34+ cells sorted after transduction or bulk cells at day 12 of culture was extracted using Direct-Zol RNA MicroPrep Kit (Zymo Research) and reverse transcription was performed with SuperScript Vilo cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: Experiments to determine whether CxNV1 has DNA forms were initiated by extracting RNA from CT cells using Direct-Zol RNA MicroPrep (Zymo Research), and separately ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested in TRIzolTM LS reagent (Invitogen) and total RNA was extracted using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instruction ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Molecular Biology 2022Quote: ... 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted using the Direct-zol DNA/RNA Miniprep kit (Zymo Research) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was isolated from a small aliquot of mixed mouse and fly cells using Quick-DNA Miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: ... the cells were washed with PBS three times and RNA was extracted using an RNA extraction kit (Zymo, Irvine, CA, USA). In a parallel experiment ...
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bioRxiv - Microbiology 2020Quote: ... were added to media-removed cell monolayers per single well of 12 well plates followed by on-column DNA digestion and purification (Zymo Research). For RNA purification from RNP capture sample ...
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: Cells were infected with 1 MOI of TB40/EGFP and RNA was isolated using Quick-DNA/RNA miniprep kit (Zymo Research) from 0-72 hpi ...
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bioRxiv - Genomics 2021Quote: ... The remaining cells were lysed with Zymo QuickRNA lysis buffer and isolated with the Zymo QuickRNA micro kit (Zymo, Cat #R1050) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus preparation or supernatants of virus-infected cells using a viral RNA extraction kit (Quick-RNA Viral Kit, R1035, Zymo Research) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... human islets or EndoC-βH5 cells using a commercial column-based purification with DNAse I treatment (Direct-Zol RNA MicroPrep with Trizol Reagent, Zymo Research). RNA was quantified by Nanodrop spectrophotometer (ThermoFisher Scientific) ...