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Citations for Zymo Research :
601 - 650 of 776 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... genomic DNA from euploid 46,XX cells was bisulfite-converted using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA), labeled and hybridized using the Infinium Methylation EPIC BeadChip Kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from infected cells in the 96-well plate using a ZR-96 Viral RNA Kit (Zymo Research) per manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from mixtures of KU-1 or BL3.1 and BTL26 for a total of 1 × 106 cells using Quick-DNA Miniprep Kits (Zymo Research). The DNA concentrations of the samples were measured by UV absorbance at 260 nm using a NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... we collected the cells and extracted genomic DNA with a commercial kit (Zymo Quick DNA miniprep Plus Kit, Cat#D4068).
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was purified from WT and Chr4-cl KO ES cells using the Quick-gDNA purification kit (Zymo Research) and bisulfite-converted with the NEXTflex™ Bisulfite-Seq Kit (Bio Scientific ...
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bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed once with 1x PBS and RNA was harvested by adding Trizol reagent (Zymo Research, Irvine, CA, USA). RNA was isolated using Direct-Zol RNA MiniPrep kit (Zymo Research ...
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bioRxiv - Biophysics 2021Quote: ... Sufficient amounts of DNA were obtained growing 1 L of transfected XL1 Blue Escherichia coli cells overnight and performing a gigaprep (Zymo). For protein expression ...
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bioRxiv - Genomics 2021Quote: Total RNA was purified in duplicate from 1 million frozen cells using the Direct-zol RNA MiniPrep kit from Zymo Research (#R2050) ...
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bioRxiv - Genomics 2021Quote: Total RNA was purified from the 10 replicate cell cultures using a Direct-zol RNA Miniprep Plus kit (Zymo Research), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... total RNA from approximately 1 × 108 cells was extracted using the Quick-RNA Fungal/Bacterial Miniprep (Zymo Research, Irvine, CA). For each sample ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from different PCa cell lines using Direct-zol quick miniprep plus RNA extraction kit (Zymo Research). cDNA was synthesized from extracted RNA using RT2 First Strand Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... see below) to which we added 50 μl of ZymoBIOMICS Microbial Community Standard Cells (Zymo Research Corp., Irvine, CA, USA). Mock sap samples were then processed as indicated above for the PowerPlant DNA extraction protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... total RNA was extracted from PDX cell pellets using the Direct-zol RNA miniprep kit (R2060, Zymo Research, Irvine, CA), with subsequent quantification using Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells were resuspended in 100uL TRIzol and RNA was extracted using the DirectZOL RNA Microprep kit (Zymo Research, #R2062). 100ng of total RNA was then used for cDNA synthesis and 25ng of cDNA was used for RT-PCR analysis ...
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bioRxiv - Cell Biology 2020Quote: HEK-293 FCγ cell mRNA was harvested and isolated using Direct-zol™ RNA Miniprep Plus (Zymo Research, Irvine, CA) according to the manufactures protocol ...
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bioRxiv - Genomics 2021Quote: Total RNA was isolated from cells and purified using Direct-zol RNA Microprep columns according to the manufacturer’s instructions (Zymo Research). 500 ng of total RNA were used to prepare sequencing libraries from polyA enriched mRNA as previously described (Link ...
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bioRxiv - Immunology 2022Quote: RNA isolation of the FACS-purified GFP+ Treg cells was performed by the Quick-RNA Microprep kit following the manufacturer’s protocol (Zymo Research). RNA quality control was performed with the Agilent 4200 Tape Station system and only RNAs having a RIN >8 were used for library preparation ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from the pelleted cells using a Quick RNA Fungal/Bacterial Microprep kit (Zymo Research, Irvine, CA, USA). The cells were mechanically lysed with Mini-Beadbeater-96 (Biospec Products ...
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bioRxiv - Microbiology 2022Quote: ... and gDNA was extracted from cell pellets by the CTAB-lysozyme method (80) and cleaned using the Genomic DNA clean and concentrator kit (Zymo) before submitting to UMGC for Tn-seq library preparation and Illumina sequencing.
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bioRxiv - Molecular Biology 2022Quote: RNA from resting B cells isolated from three wild-type animals was isolated using Direct-zol RNA Miniprep Kit (Zymo). Libraries were constructed using the Illumina TruSeq Stranded Total RNA Library and sequenced on NextSeq2000 with PE100 sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and DNA was extracted at days 1 and 5 utilizing the Quick-DNA/RNA Miniprep (Zymo Research). Viral genomes were quantified using a standard curve and normalized to RNAseP.
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bioRxiv - Synthetic Biology 2023Quote: Highly competent BL21(DE3) cells expressing chaperone pGro7 (BL21(DE3)-pGRO7) were prepared in-house using a kit from Zymo Research (kit # T3002) ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... and Δcpc1CPC1 parasites were grown in HFF cells for 48 hrs and filter-purified for total RNA extraction using a Direct-zol RNA MiniPrep Plus kit (Zymo). Transcript levels of individual genes were determined by the Luna Universal One-Step RT-PCR kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... the cells and medium were harvested and subjected to RNA extraction using the Direct-zol RNA MiniPrep Kit (Zymo Research) or Direct-zol-96 RNA Kit (Zymo Research) ...
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bioRxiv - Microbiology 2023Quote: ... Transformation of yeast cells was performed according to the manufacturer’s guidelines using the Frozen-EZ Yeast Transformation Kit (Zymo Research). Genes of interest were amplified from wild-type genomic DNA using Phusion Polymerase (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated from control and CIC knock out cell lines following treatment with DMSO or 40 uM forskolin for 48 hours using Quick-RNA MiniPrep (Zymo). The University of Minnesota Genomics Core assessed sample quality using Agilent BioAnalyzer 2100 and all samples were found to have an RNA Integrity Number (RIN ...
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bioRxiv - Genetics 2023Quote: ... the genomic DNA was extracted from the CRISPR screen cell library using Zymo Quick-DNA Miniprep Kit (Zymo Research, D4068) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed in Tri-reagent and RNA was extracted using the Direct-zol RNA miniprep kit (Zymo Research, # R2050) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were prepared and transformed with plasmids using the Mix and Go™ kit (Zymo Research, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Genomics 2023Quote: ... lysate from ∼1×106 cells in the well of a 6-well plate was collected in 300 µL of DNA/RNA Shield (Zymo). RNA was isolated using the Quick-RNA Miniprep Plus Kit (Zymo ...
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bioRxiv - Neuroscience 2023Quote: ... A small volume (∼10µl) of the dissociated cells was used for DNA isolation by using Quick-DNA Microprep Kit (Zymo research). The DNA was amplified by PCR and sequenced via Sanger Sequencing ...
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bioRxiv - Genetics 2024Quote: ... A cell line was prepared encoding this donor plasmid by transforming BL21(DE3) and making the resultant cell line chemically competent using Mix & Go preparation kit (Zymo). The temperature sensitive donor plasmid was then transformed with a second plasmid encoding a T7-inducible recombinase and a constitutively expressed bridge RNA ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA of the cells transduced with the sgRNA library were extracted with Genomic DNA Clean & Concentrator (Zymo Research D4010). We amplified 100 ng genomic DNA with the same two-round strategy ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were infected with 229E at an MOI of 1.0 and were lysed with RNA lysis buffer (Zymo# R1035) at 1 ...
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bioRxiv - Cell Biology 2023Quote: DNA extraction from whole input cells and purified nucleoli was performed using a Quick-DNA Universal Kit (Zymo Research, CA). DNA concentration was measured using a Qubit dsDNA HS Assay kit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from MV4;11 Cas9 + GeCKOv2 cells collected before and following sustained C6-treatment using the Quick-gDNA MidiPrep Kit (Zymo Research) per manufacturer’s directions ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were scraped into solution and total RNA was purified using Zymo Quick-RNA Miniprep kit (Zymo Research, R1054). Of the isolated total RNA ...
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bioRxiv - Cancer Biology 2021Quote: Sorted cKithighCD11blow AML cells were lysed in Trizol reagent and RNA was extracted using Direct-zol RNA miniprep kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from approximately 105 − 106 cells was extracted from the cultures of the evolution experiments every 48 hours using Quick-DNA miniprep kits (Zymo Research). The HIV RT gene amplified by PCR from the proviral RNA using Phusion hot start II DNA polymerase (New England Bio-labs ...
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Molecular Biology 2021Quote: ... reporter assay was carried out as described above in presence of Nsp1 or Nsp2 followed by harvesting of cells in Trizol (Zymo Research). The RNA was isolated using Direct-zol RNA miniprep kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from the sorted or the control cells either using the Quick-DNA Mini Kit or the Midi Plus Kit (Zymo Research). The integrated LentiCRISPRv2 vector sgRNA sequences were amplified through three rounds of PCR using the Taq DNA Polymerase S (high specificity ...
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bioRxiv - Cell Biology 2022Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
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bioRxiv - Genomics 2020Quote: ... DNA was then extracted from approximately 1 ml of the red blood cell pellets using Zymo Bead Genomic DNA kit (Zymo Research) following the manufacturer’s procedures ...
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bioRxiv - Cell Biology 2019Quote: ... Genotyping PCR was performed using Taq polymerase on genomic DNA extracted from SMB55 cells using ZymoBead™ Genomic DNA kit (Zymo). Genomic DNA extracted from Ptch1-/- mouse embryonic fibroblasts and a wild-type mouse were used as a control for the mutant and wild-type alleles respectively ...