Labshake search
Citations for Zymo Research :
6401 - 6450 of 6869 citations for Oxytocin ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 2% Agarose gel and the band at 150 bp was excised and then purified using the Zymoclean Gel DNA Recovery kit (Zymo Research). The dgRNA was synthesized via IVT using the MEGAscript T7 Transcription kit supplemented with 10mM ATP ...
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bioRxiv - Genomics 2023Quote: ... was transformed with 2 uL of each ligation reaction and resulting colonies were selected for plasmid DNA isolation using the ZymoPure Plasmid miniprep kit (Zymo Research). Each cloned gRNA sequence-containing PB-CRISPRia plasmid was verified by Sanger sequencing (Quintara Bio).
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bioRxiv - Microbiology 2023Quote: ... the same PCR fragment used for Sanger sequencing was purified using a ZymocleanTM Gel DNA Recovery Kit (Zymo Research, cat# D4002). DNA (∼1 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... gel fragments corresponding to 190 – 350 nts in DNA size were excised and libraries extracted using the Zymoclean Gel DNA Recovery Kit (D4007, Zymo Research). DNA concentrations were measured using the Qubit dsDNA HS Assay Kit (Q32854 ...
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bioRxiv - Plant Biology 2023Quote: ... and was size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research, Irvine, CA. USA). The final library was sequenced using a 2 × 150 bp paired-end protocol on the NovaSeq 6000 system (Illumina).
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bioRxiv - Microbiology 2023Quote: ... and PPL554 of bacterial cultures grown overnight in NB media overnight was extracted using the QuickDNA™ Fungal/Bacterial Miniprep Kit sourced from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: Stored bacterial pellets were used for RNA extraction using the Quick-RNA Fungal/Bacterial Miniprep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: RNA from exponentially growing or senescent human and bowhead whale primary skin fibroblasts was isolated using the Quick-RNA MiniPrep kit (Zymo Research) according to manufacturer instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... were transformed into Saccharomyces cerevisiae VL6-48(Yamanaka et al., 2014) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). The correct promoter insertion was screened by PCR using primers upstream and downstream of the target site (Table S4) ...
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bioRxiv - Cancer Biology 2023Quote: ... Modifications to the published method are the following: 1) extraction of RNA prior to cDNA reverse transcription with the Zymo Direct-zol-96 RNA isolation kit (Zymo, #R0256) 2 ...
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bioRxiv - Plant Biology 2024Quote: ... was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research). Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Plant Biology 2024Quote: ... A 200 mg soil sample from each falcon tube was taken for DNA extraction using the Zymo Quick DNA Fecal/Soil Microbe MiniPrep Kit (Zymo Research). Before sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... which were removed from the mutational libraries by gel purification using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Purified products were again transformed into either NEB 10-beta (New England Biolabs ...
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bioRxiv - Physiology 2024Quote: ... The total RNA of isolated renal tubules was immediately extracted using Quick-RNA™ MicroPrep kit (Zymo Research, Irvine, CA, USA). Reverse transcription was performed using Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research; Tustin, CA). Eluted RNA was analyzed in a Nanodrop Lite (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: We used a Trizol-based protocol to extract RNA from primate samples using the Direct-zol RNA Microprep kit (R2060, Zymo Research). All steps were centrifuged at 13,000 g for 30 s unless otherwise noted ...
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bioRxiv - Microbiology 2024Quote: ... S4) and cells were collected by filtration for RNA purification using the RNA Clean and Concentrator kit (Zymo Research, Irvine CA). rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre ...
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bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA around binding sites was cleaved with pAG-MNase enzyme and the released DNA was reverse-crosslinked with proteinase K and 0.1% SDS overnight at 65°C before purification with a ChIP DNA Clean & Concentrator kit (Zymo Research D5205). DNA was converted into Illumina-compatible libraries with the NEBNext(R ...
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bioRxiv - Cell Biology 2024Quote: Yeast transformations were performed either using a standard lithium acetate transformation or Zymo Frozen-EZ Yeast Transformation II Kit (Zymo #T2001) before plating on appropriate selection media82 ...
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bioRxiv - Microbiology 2024Quote: ... The upper aqueous phase was transferred to an RNase-free tube and mixed in a 1:1 ratio with 100% EtOH and processed via the clean and concentrator kit (Zymo Research). RNA was eluted in 15 µL RNase-free ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... 250 µl aqueous phase was mixed 1:1 with 75% ethanol and transferred into the Zymo-Spin IC Column from the RNA Clean & Concentrator Kit (Zymo Research). RNA clean-up and collection was done following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: RNA was extracted from callus and protoplast cultures using the ZR Plant RNA Miniprep™ kit (R2024, Zymo Research, CA, USA) and TRIzol™ Reagent (15596026 ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 1.5 mL cultures of Bacteroides strains using the Zymobionics DNA Miniprep kit (Zymo Research, Irvine, CA) and used as input for IS-Seq as described previously 19 ...
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bioRxiv - Genomics 2024Quote: ... Total RNA (∼700 ng) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA was mixed with ethanol absolute at a 1:1 v/v ratio and submitted to a spin column-based purification protocol (Direct-zol RNA Miniprep kit, Zymo Research), including a DNAse treatment step ...
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bioRxiv - Genomics 2024Quote: gDNA was isolated from ∼25 mg of frozen tissue or ∼1 × 106 HepG2 cells suspended in 200 µL of PBS using the Quick-DNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol with an overnight (for frozen tissue ...
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bioRxiv - Genetics 2024Quote: ... followed by resuspension of ∼200k to 1 million cells in 300ul RNA Lysis Buffer from the Quick-RNA Miniprep Kit (Zymo R1055) and stored in −70deg ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA from the heart and testis of the Madagascar ground gecko was purified using ISOGEN (#311-02501, Nippon Gene) and Direct-zol RNA Miniprep kit (#R2050, Zymo Research). The cDNA was synthesized from total RNA using Verso cDNA synthesis kit (#AB1453A ...
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bioRxiv - Cell Biology 2024Quote: ... RNA from tdTomato positive SRSF2 knockout and P95H mutant cells and reporter positive control cells was extracted using the Direct-zol RNA microprep kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted using the manufacturer’s instructions using a YeaStar Genomic DNA kit (Catalog D2002, Zymo Research, Irvine, California, USA). Genomic DNA was subject to a PCR to check for the insertion of the corresponding tag or the CHC KO.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from cells expressing the iRS3 reporter 3h post-induction using the Direct-zol™ RNA MiniPrep kit (Zymo). After extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from infected cells 72 h post infection using Quick-DNA Miniprep kit (Zymo Research, catalogue no. D3024).
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bioRxiv - Genomics 2024Quote: ... An aliquot of 250 ng DNA from each sample was bisulfite converted using the EZ DNA Methylation kit (Zymo Research, #D5001) according to manufacturer’s instructions for Illumina DNA methylation arrays ...
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bioRxiv - Plant Biology 2024Quote: Preparation of RNA samples from seedlings of the indicated genotypes and growth conditions was performed using a Quick-RNA MiniPrep Kit with on-column DNase I treatment (Zymo Research). cDNA synthesis was performed with 1.5 µg of total RNA using a Superscript IV First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... contains the Arabidopsis thaliana cytochrome P450 reductase I gene, ATR1) (Urban et al., 1997) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For plant transient expression ...
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bioRxiv - Genetics 2024Quote: ... Two micrograms of DNA were subjected to sodium bisulfite conversion using an EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA). Genomic regions were subsequently amplified using EpiTaq HS (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was collected over a time course of 24 hours and isolated using a Quick-DNA/RNA™ Miniprep kit (Zymo) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µl of RNase free water was added to the mixture and transcribed RNA was eluted using the RNA Clean & Concentrator kit (Zymo Research). Samples were assessed for their length and concentration by Bioanalyzer (Agilent ...
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bioRxiv - Zoology 2024Quote: ... and post-aestivating (55 days old) female beetles were extracted using the Quick-RNA Tissue/Insect Kit (Zymo Research, Freiburg, Germany). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of ethanol were added to each of the samples before eluting the RNA using a Direct-zol RNA kit (Zymo Research) using manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Following MASC PCR validation using the MASC PCR primer set described in Supplementary Data 1 and BAC extraction using the ZR BAC DNA Miniprep Kit (Zymo Research) based on the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated from freshly-grown colonies using the Zymo Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA). Libraries were prepared using the Nextera DNA Flex Library Prep as recommended by the manufacturer (Illumina ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from 1*106 WT or sorted SLAMF6-KO NK-92 cells using an RNA Micro Kit (Zymo Research). After PolyA selection libraries were prepared and lllumina sequencing of 20-30 million paired-end reads performed ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and RNA was isolated using Direct-zol RNA miniprep Kit (Zymo research, cat. no. R2050). RNA was quantified and processed for cDNA synthesis using Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria expressing the correct plasmid were grown in bulk and high-purity plasmid was extracted using ZymoPURE Plasmid Midiprep kit (Zymo research) and verified by sequencing of the coding region of the influenza virus segment.
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bioRxiv - Developmental Biology 2024Quote: Total RNA was isolated from 1-day old zebrafish embryos with TRI Reagent using Direct-zolTM RNA MicroPrep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: Yeast genomic DNA was extracted from the potential aMF biosensor yeast strains and the unedited yWS677 strain (to be used as negative control in PCR analysis) using the YeaStar Genomic DNA kit and protocol (Zymo Research), with no known deviations.
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bioRxiv - Plant Biology 2020Quote: ... and then both ribosome-bound RNA and denatured total RNA samples were purified with RNA Clean & concentrator-25 kit (Zymo Research, USA). RPFs were size-fractionated on 15% urea polyacrylamide gel electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...