Labshake search
Citations for Zymo Research :
6201 - 6250 of 6483 citations for Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from the livers or adipose tissue using Direct-zol RNA Microprep Kits (R2061, Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Antibody genes were isolated from the terminal rounds of sorting against tau and α-synuclein fibrils using a yeast miniprep kit (Zymo, D2004). For tau ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR were performed and PCR amplicons were used to conduct gel running and purified by DNA Clean & Concentrator kit (Zymo Research, # D4031). A-tailing were then performed on the purified PCR products following the NEB protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA was then isolated from the membrane using phenol-chloroform and concentrated with the RNA Clean and Concentrator kit (Zymo Research, R1013), followed by reverse transcription using the RT Oligo primer (Supplementary Data 12 and SuperScript III ...
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bioRxiv - Plant Biology 2023Quote: ... and prey (pGADT7) constructs were co-transformed into the yeast strain AH109 using Frozen-EZ Yeast Transformation II Kit (Zymo Research, T2001). The resulting transformants were spotted on SD (−Leu ...
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bioRxiv - Genomics 2023Quote: ... and dissected tissues (head, abdomen, gonads) from adult unparasitised males and females using a Direct-zol RNA Microprep kit (Zymo Research, USA), including DNAse treatment following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... in Lysing Matrix B tubes and RNA was purified with the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA, United States). The quantity and quality of the RNAs were evaluated using Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were then run on a 10% TBE polyacrylamide gel and purified using the DNA Clean & Concentrator-5 kit (Zymo Research D4013).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... ligation products were cleaned using AMPure XP Beads (Fisher, Cat. #NC9933872) before processing for bisulfite conversion using the Zymo EZ DNA Methylation Kit (Zymo, Cat. #D5001), and by amplifying the bisulfite converted products over 55 cycles of 95°C for 30 seconds followed by 55°C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA from line with Bon GLKD driven by MT-Gal4 (and control flies that express an shRNA against the white gene) was depleted of ribosomal RNA with the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, #R3000). RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Genomics 2023Quote: ... Single colonies were used to inoculate ampicillin liquid cultures grown overnight with shaking at 37°C and plasmids were subsequently purified with the ZR Plasmid Miniprep kit (Zymo Research #D4016). The purified plasmids were screened by Sanger sequencing (GeneWiz ...
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bioRxiv - Genetics 2023Quote: Genomic DNA (gDNA) was isolated from two G1 adults per mutant line using Zymo Quick-DNA MicroPrep kit (Zymo Research, Irvine, CA), and the yield was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2023Quote: ... ∼15 ng of genomic DNA from each sample was first spiked in with in vitro methylated lambda phage genomic DNA (0.2%) as controls and was then subjected to bisulfite conversion (EZ DNA Methylation-Direct Kit, Zymo Research Cat# D5020). Half of the bisulfite converted DNA was used for low input WGBS analysis (for 5mC+5hmC profiling) ...
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bioRxiv - Genomics 2023Quote: 10 ng of gDNA isolated from GFP+/DAPI-FACS-sorted cell populations was first spiked with lambda phage controls and was bisulfite-converted with the Zymo EZ DNA Methylation Direct Kit (Zymo Research, D5020) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from GFP+/DAPI-FAC-sorted transient transfected HEK293T cells with ZR-Duet DNA/RNA MiniPrep Kit (Zymo Research, D7001). RNA-seq libraries were prepared using SMARTer Stranded total RNA-seq Kit v3 (Clontech ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... 40 IVO blastocysts or 80 8-cell embryos were digested and bisulfite converted according to the manufacturer’s instruction of EZ DNA Methylation-Direct Kit (Zymo, Irvine, CA, USA). Bisulfite-specific primers were designed with the online Methyl Primer Express ...
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Biochemistry 2023Quote: ... DNA of 86 ovarian cancer patients was isolated from paraffin-embedded normal tissue with Quick-DNA FFPE Miniprep kit (Zymo Research, USA); other DNA samples were isolated from blood using PureLink Genomic DNA Mini Kit (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The cDNA was size selected using denatured polyacrylamide gel electrophoresis followed by dPAGE purification (small-RNA PAGE Recovery Kit, Zymo Research #R1070). The cDNA was then amplified using Q5-Ultra II DNA polymerase (New England Biolabs #M0544 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by end-repair using T4 PNK after purification of the rRNA depleted samples using Oligo Clean & Concentrator Kits (Zymo research, #D4060). The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB ...
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bioRxiv - Paleontology 2024Quote: ... qPCR was used to calculate the absolute number of bacterial cells in each sample by targeting the 16S rRNA gene using a Femto Bacterial DNA Quantification Kit (Zymo Research International). DNA sequencing was also carried out on a negative control (no contamination was found) ...
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bioRxiv - Molecular Biology 2024Quote: The RNA was extracted and purified using the Directzol RNA MiniPrep kit including the recommended DNase I treatment (Zymo Research; Cat# R2052) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: RNA from biological duplicates for each condition (more than 30,000 cells for each sample) was extracted using the Quick-RNA Miniprep Kit (Zymo; Cat. No. R1055). Libraries were prepared from total RNA (15 ng per sample ...
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bioRxiv - Genomics 2024Quote: ... Total genomic DNA was extracted from thallus fragments using the ZR Fungal/Bacterial DNA Miniprep Kit (Zymo Research Corp., Irvine, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Five hundred nanograms of each DNA sample and control DNA were bisulfite-treated (BST) using the EZ DNA Methylation-Lightning Kit (Zymo Research, # D5030) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled in Tris-EDTA containing 1% SDS and 12% β-Mercaptoethanol before RNA was extracted using a Direct-zol RNA Microprep kit (Zymo Research). The extracted RNA was then prepared for RT- qPCR analysis to examine RB1 ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences) to confirm successful gene insertion and MaxiPrepped using the ZymoPURE II™ Plasmid Maxiprep kit (Zymo, #D4202) before transfection into Expi293™ cells.
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bioRxiv - Neuroscience 2024Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Vendor-designed primers for bacterial 16S amplified the V3-V4 region ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences) to confirm presence of desired mutation and MaxiPrepped using the ZymoPURE II™ Plasmid Maxiprep kit (Zymo, #D4202) before transfection into Expi293™ cells.
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from the mixture of colonies from each plate using a Quick-DNA HMW MagBead Kit (Zymo Research, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Libraries were generated by the Emory Integrated Genomics Core (EIGC) as follows: RNA was isolated using the Quick-RNA MicroPrep kit (Zymo,11-328M). 2000 cell equivalent of RNA was used as input to SMART-seq v4 Ultra Low Input cDNA Synthesis kit (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... ssDNA of the bound phages was collected using a modified protocol from the Quick-DNA/RNA Miniprep Kit (Zymo Cat No. D7011). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted by phenol-chloroform for the ZNF469 profiling to keep the small DNA fragments and with the DNA Clean & Concentrator-5 kit (Zymo Research, D4004) for the H3K27ac profiling ...
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bioRxiv - Genetics 2024Quote: ... GMSM-K - 6 non-risk and 7 risk genotype for rs11119348 and 4 each genotype for rs661849) using Quick-DNA/RNA Miniprep kit (Zymo Research #D7001) followed by DNase treatment using manufacturer’s instructions (Thermo Fisher Scientific #AM1907) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmids were then transformed into this yeast strain using the standard LiAc method (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and selected for on SD-trp ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The inserts were verified with PCR and Sanger sequencing and then transformed into the yeast hosts using either the standard LiAc protocol (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and plated onto SD-leu media.
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bioRxiv - Molecular Biology 2024Quote: ... Fragments were then separated by gel electrophoresis and the band corresponding to digested vector was extracted using a Zymo kit (Zymo cat#D4002) and eluted into 20 μL nuclease-free water.
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bioRxiv - Physiology 2024Quote: ... WT and Rnlsmut NIT-1 cells were used for RNA isolation using Zymo Quick-RNA miniprep plus kit (Zymo Research cat. R1058). Novogene Corporation Inc ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from faecal samples using the ZymoBIOMICS DNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, California, USA). DNA was then bisulfite-converted using the Zymo EZ DNA Methylation-Gold Kit to convert unmethylated cytosines to uracil and then thymine (Zymo Research ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from different tissues (gills, gonads, skin, liver, eyes, muscle) from one male individual by using the Quick-RNA MiniPrep plus Kit (Zymo Research, USA). Tissues were placed in tubes filled with 800 μl DNA/RNA shield and lysed via bead beating (speed 4 M/S ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted and DNAseI-treated from DAC-treated AF2 cells using the ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003). This RNA was converted to cDNA using SuperScript III First-Strand Synthesis System (Invitrogen Cat# 18080051 ...
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bioRxiv - Genetics 2021Quote: ... 500 ng of buffy coat DNA was hybridized to the Methyl450 array after bisulfite conversion with EZ DNA kits (Zymo Research, Irvine, CA) prior to standard Illumina amplification ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was conducted using a CTAB protocol combined with the Zymo DNA clean and concentrator kit (Zymo Research Corp., Orange, CA, USA) as reported previously [71] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Microbial DNA extractions were performed in batches (stored on ice) immediately after intestinal dissections with the Zymobiomics DNA Miniprep Kit (Zymo Research, Irvine, CA).
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bioRxiv - Developmental Biology 2020Quote: RNA was isolated directly from the TRIzol muscle samples using a 96 well plate extraction kit (Direct-zol™-96 RNA, Zymo Research, #R2054): after thawing to room temperature in 1,5 ml Eppendorf tubes ...