Labshake search
Citations for Zymo Research :
6201 - 6250 of 6483 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... and prey (pGADT7) constructs were co-transformed into the yeast strain AH109 using Frozen-EZ Yeast Transformation II Kit (Zymo Research, T2001). The resulting transformants were spotted on SD (−Leu ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA from line with Bon GLKD driven by MT-Gal4 (and control flies that express an shRNA against the white gene) was depleted of ribosomal RNA with the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, #R3000). RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Plant Biology 2024Quote: Total RNA from seedlings was isolated using a Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, Irvine, CA). cDNA was synthesized using Oligo(dT ...
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bioRxiv - Physiology 2024Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick-RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Genomics 2023Quote: ... ∼15 ng of genomic DNA from each sample was first spiked in with in vitro methylated lambda phage genomic DNA (0.2%) as controls and was then subjected to bisulfite conversion (EZ DNA Methylation-Direct Kit, Zymo Research Cat# D5020). Half of the bisulfite converted DNA was used for low input WGBS analysis (for 5mC+5hmC profiling) ...
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bioRxiv - Genomics 2023Quote: 10 ng of gDNA isolated from GFP+/DAPI-FACS-sorted cell populations was first spiked with lambda phage controls and was bisulfite-converted with the Zymo EZ DNA Methylation Direct Kit (Zymo Research, D5020) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from GFP+/DAPI-FAC-sorted transient transfected HEK293T cells with ZR-Duet DNA/RNA MiniPrep Kit (Zymo Research, D7001). RNA-seq libraries were prepared using SMARTer Stranded total RNA-seq Kit v3 (Clontech ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Biophysics 2023Quote: ... The cDNA was size selected using denatured polyacrylamide gel electrophoresis followed by dPAGE purification (small-RNA PAGE Recovery Kit, Zymo Research #R1070). The cDNA was then amplified using Q5-Ultra II DNA polymerase (New England Biolabs #M0544 ...
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Biochemistry 2023Quote: ... DNA of 86 ovarian cancer patients was isolated from paraffin-embedded normal tissue with Quick-DNA FFPE Miniprep kit (Zymo Research, USA); other DNA samples were isolated from blood using PureLink Genomic DNA Mini Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... in Lysing Matrix B tubes and RNA was purified with the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA, United States). The quantity and quality of the RNAs were evaluated using Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and dissected tissues (head, abdomen, gonads) from adult unparasitised males and females using a Direct-zol RNA Microprep kit (Zymo Research, USA), including DNAse treatment following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 vRNA was isolated from BAL supernatant (200 µL) and mucosal swabs (nasal, pharyngeal, and rectal) using the Zymo Quick-RNA Viral Kit (Zymo Research, USA) as previously described [27,29] ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from the livers or adipose tissue using Direct-zol RNA Microprep Kits (R2061, Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The MDA-amplified VLP DNA was then processed using the Zymo Genomic DNA Clean and Concentrator-10 kit (Zymo Research, CA, U.S.A) and eluted in Zymo DNA Elution Buffer ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Microbiology 2023Quote: Viral RNA extraction from nasal washes of infected ferrets was done using the Quick-RNA Viral 96 Kit (Zymo, Cat. No. R1040) using 160 µL sample volume ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... T7 transcribed SL5 RNA was first purified by polyacrylamide gel electrophoresis (PAGE) and recovered using small-RNA PAGE Recovery Kit (Zymo Research, R1070). RNase L (1.3 µg in 5 µL ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from the mixture of colonies from each plate using a Quick-DNA HMW MagBead Kit (Zymo Research, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... GMSM-K - 6 non-risk and 7 risk genotype for rs11119348 and 4 each genotype for rs661849) using Quick-DNA/RNA Miniprep kit (Zymo Research #D7001) followed by DNase treatment using manufacturer’s instructions (Thermo Fisher Scientific #AM1907) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The inserts were verified with PCR and Sanger sequencing and then transformed into the yeast hosts using either the standard LiAc protocol (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and plated onto SD-leu media.
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmids were then transformed into this yeast strain using the standard LiAc method (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and selected for on SD-trp ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from faecal samples using the ZymoBIOMICS DNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, California, USA). DNA was then bisulfite-converted using the Zymo EZ DNA Methylation-Gold Kit to convert unmethylated cytosines to uracil and then thymine (Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by end-repair using T4 PNK after purification of the rRNA depleted samples using Oligo Clean & Concentrator Kits (Zymo research, #D4060). The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... ssDNA of the bound phages was collected using a modified protocol from the Quick-DNA/RNA Miniprep Kit (Zymo Cat No. D7011). Briefly ...
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bioRxiv - Paleontology 2024Quote: ... qPCR was used to calculate the absolute number of bacterial cells in each sample by targeting the 16S rRNA gene using a Femto Bacterial DNA Quantification Kit (Zymo Research International). DNA sequencing was also carried out on a negative control (no contamination was found) ...
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bioRxiv - Neuroscience 2024Quote: RNA from biological duplicates for each condition (more than 30,000 cells for each sample) was extracted using the Quick-RNA Miniprep Kit (Zymo; Cat. No. R1055). Libraries were prepared from total RNA (15 ng per sample ...
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bioRxiv - Molecular Biology 2024Quote: The RNA was extracted and purified using the Directzol RNA MiniPrep kit including the recommended DNase I treatment (Zymo Research; Cat# R2052) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Total genomic DNA was extracted from thallus fragments using the ZR Fungal/Bacterial DNA Miniprep Kit (Zymo Research Corp., Irvine, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...
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bioRxiv - Neuroscience 2024Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Vendor-designed primers for bacterial 16S amplified the V3-V4 region ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled in Tris-EDTA containing 1% SDS and 12% β-Mercaptoethanol before RNA was extracted using a Direct-zol RNA Microprep kit (Zymo Research). The extracted RNA was then prepared for RT- qPCR analysis to examine RB1 ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences) to confirm successful gene insertion and MaxiPrepped using the ZymoPURE II™ Plasmid Maxiprep kit (Zymo, #D4202) before transfection into Expi293™ cells.
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences) to confirm presence of desired mutation and MaxiPrepped using the ZymoPURE II™ Plasmid Maxiprep kit (Zymo, #D4202) before transfection into Expi293™ cells.
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bioRxiv - Cancer Biology 2024Quote: ... Five hundred nanograms of each DNA sample and control DNA were bisulfite-treated (BST) using the EZ DNA Methylation-Lightning Kit (Zymo Research, # D5030) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted by phenol-chloroform for the ZNF469 profiling to keep the small DNA fragments and with the DNA Clean & Concentrator-5 kit (Zymo Research, D4004) for the H3K27ac profiling ...
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bioRxiv - Molecular Biology 2024Quote: ... Fragments were then separated by gel electrophoresis and the band corresponding to digested vector was extracted using a Zymo kit (Zymo cat#D4002) and eluted into 20 μL nuclease-free water.
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bioRxiv - Physiology 2024Quote: ... WT and Rnlsmut NIT-1 cells were used for RNA isolation using Zymo Quick-RNA miniprep plus kit (Zymo Research cat. R1058). Novogene Corporation Inc ...
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bioRxiv - Immunology 2024Quote: ... Libraries were generated by the Emory Integrated Genomics Core (EIGC) as follows: RNA was isolated using the Quick-RNA MicroPrep kit (Zymo,11-328M). 2000 cell equivalent of RNA was used as input to SMART-seq v4 Ultra Low Input cDNA Synthesis kit (Takara ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from different tissues (gills, gonads, skin, liver, eyes, muscle) from one male individual by using the Quick-RNA MiniPrep plus Kit (Zymo Research, USA). Tissues were placed in tubes filled with 800 μl DNA/RNA shield and lysed via bead beating (speed 4 M/S ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted and DNAseI-treated from DAC-treated AF2 cells using the ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003). This RNA was converted to cDNA using SuperScript III First-Strand Synthesis System (Invitrogen Cat# 18080051 ...
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bioRxiv - Genetics 2021Quote: ... 500 ng of buffy coat DNA was hybridized to the Methyl450 array after bisulfite conversion with EZ DNA kits (Zymo Research, Irvine, CA) prior to standard Illumina amplification ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was conducted using a CTAB protocol combined with the Zymo DNA clean and concentrator kit (Zymo Research Corp., Orange, CA, USA) as reported previously [71] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Microbial DNA extractions were performed in batches (stored on ice) immediately after intestinal dissections with the Zymobiomics DNA Miniprep Kit (Zymo Research, Irvine, CA).
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bioRxiv - Developmental Biology 2020Quote: RNA was isolated directly from the TRIzol muscle samples using a 96 well plate extraction kit (Direct-zol™-96 RNA, Zymo Research, #R2054): after thawing to room temperature in 1,5 ml Eppendorf tubes ...