Labshake search
Citations for Zymo Research :
6151 - 6200 of 6492 citations for Mouse Butyrylcholinesterase BCHE CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... small RNAs ranging from 17-to-25 nt were excised and recovered using the ZR small-RNA PAGE Recovery Kit (Zymo Research, #R1070). TraPR ...
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bioRxiv - Molecular Biology 2024Quote: ... was used to purify genomic DNA from the cells followed by bisulfite conversion using 50 ng DNA along with the EZ DNA Methylation- Gold kit (Zymo Research D5005). 10 ng of bisulfite-converted DNA was used to generate the whole genome bisulfite sequencing (WGBS ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 750 ng of DNA was subjected to bisulfite conversion using the EZ DNA Methylation kit (Zymo Research, Irvine, USA). Genome-wide DNA methylation was then performed using the Infinium HumanMethylationEPIC Bead Chip (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s instructions and 35 µL of the supernatant were treated with the RNA Clean & Concentrator Kit 5 (Zymo Research, Irvine, USA) using an adapted protocol (the RNA prep buffer step was performed twice ...
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bioRxiv - Genomics 2024Quote: ... RNA and DNA were simultaneously extracted from the cell samples using the Zymo Quick-DNA/RNA Microprep Plus Kit (Zymo Research, D7005) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and all reactions with the appropriate molecular weight product were combined and cleaned using the DNA Clean and Concentrator 5 kit (Zymo Research D4013). 10 µg LentiCRISPRv2-opti (Addgene #163126 ...
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bioRxiv - Immunology 2024Quote: ... The sgRNA target sites were amplified using PCR and purified using the DNA clean and concentrate kit (Zymo Research, Cat. No. D4033). The purified PCR amplicon products were sequenced by Eurofins Genomics ...
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bioRxiv - Microbiology 2024Quote: ... The amplified products were purified using the QIAquick PCR purification kit and underwent size selection using 1.5% low-melt agarose gels and Gel DNA Recovery Kit (Cat. No. D4002, Zymo Research, Irvine, CA). A final pool was made after quantification of each sample using the Qubit high-sensitivity dsDNA kit (Cat ...
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bioRxiv - Microbiology 2024Quote: ... The amplified promoter and coding PIGJ DNA sequence were first purified from 1% agarose gels using the ZymocleanTM Gel DNA recovery kit (Zymo Research, #D4007) and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB ...
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bioRxiv - Genomics 2023Quote: ... Single colonies were used to inoculate ampicillin liquid cultures grown overnight with shaking at 37°C and plasmids were subsequently purified with the ZR Plasmid Miniprep kit (Zymo Research #D4016). The purified plasmids were screened by Sanger sequencing (GeneWiz ...
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bioRxiv - Genetics 2023Quote: Genomic DNA (gDNA) was isolated from two G1 adults per mutant line using Zymo Quick-DNA MicroPrep kit (Zymo Research, Irvine, CA), and the yield was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Developmental Biology 2023Quote: ... 40 IVO blastocysts or 80 8-cell embryos were digested and bisulfite converted according to the manufacturer’s instruction of EZ DNA Methylation-Direct Kit (Zymo, Irvine, CA, USA). Bisulfite-specific primers were designed with the online Methyl Primer Express ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets collected from 48-well plates were lysed and total RNAs were extracted using the Direct-zolTM RNA Miniprep kit (Zymo Research R2053) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... DNA extracts from each experimental replicate without standards or phage HM1 were cleaned up prior to library preparations with the Zymo Genomic DNA Clean and Concentrate-10 kit (Cat. No. D4011, Zymo Research Corporation). Long read libraries were prepared with the Ligation Sequencing Kit (Cat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... methylated indexed adapters were ligated and 20 µL of cleaned DNA fragments were subjected to sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research, CA). Pre-amplification of the bisulfite-converted library was carried out for an equimolar pool of eight samples and was then subjected to exome capture at 47°C for 45 hours ...
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bioRxiv - Neuroscience 2023Quote: Primary cortical neurons were infected at 3 DIV with FL1 and at 10-12 DIV neurons were harvested and DNA extracted using Quick-DNA Miniprep Kit (Zymo Research, USA) following manufacturer recommendations ...
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bioRxiv - Immunology 2023Quote: ... and total cellular RNA from cardiomyocytes isolated from vehicle-and Doxo-treated mice was prepared using Direct-zol RNA miniprep kit (Zymo Research, R2052). Next-generation RNA sequencing procedure for both sets of samples were performed at Texas A&M University Bioinformatics Core ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... and immediately lysed with TRIzol (Fischer Scientific, 15-596-018) for RNA isolation using the Direct-zol RNA MicroPrep Kit (Zymo Research, R2062) per the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
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bioRxiv - Cell Biology 2023Quote: ... The sorted nuclei were then pelleted at 3000 × g for 10 minutes before mRNA isolation using the Direct-zol RNA Microprep kit (Zymo research R2060). All RNA samples were initially amplified using the Ovation RNA-Seq System v2 kit (Tecan Genomics) ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR were performed and PCR amplicons were used to conduct gel running and purified by DNA Clean & Concentrator kit (Zymo Research, # D4031). A-tailing were then performed on the purified PCR products following the NEB protocol ...
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bioRxiv - Bioengineering 2023Quote: Antibody genes were isolated from the terminal rounds of sorting against tau and α-synuclein fibrils using a yeast miniprep kit (Zymo, D2004). For tau ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Genomics 2023Quote: ... 200 ng of sonicated DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold™ Kit (Zymo, Cat No. D5006). Libraries were then made using the Accel-NGS Methyl-Seq DNA Library Kit with unique dual indices following the size-selection guidance provided with the kit and 10 cycles of amplification to minimise clonality ...
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Genetics 2023Quote: RNA was extracted from each of the 384 samples in batches of 30 samples using the Direct-zol RNA Miniprep kit (Zymo Research, R2050). Batches consisted of samples from the same tissue and were processed in the order individual samples were collected ...
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bioRxiv - Genomics 2023Quote: ... bisulfite sequencing libraries were constructed according to the manufacturer’s instructions using the EZ DNA Methylation-GoldTM Kit (Zymo Research, Irvine, CA, USA). Bisulfite sequencing libraries were sequenced to generate 150 bp paired-end reads ...
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bioRxiv - Cancer Biology 2023Quote: In vitro-infected keratinocytes and SCC cells were lysed in 6-wells with 1mL TRIzol Reagent and the RNA was purified by using the Direct-zol™ RNA Miniprep kit (Zymo Research). The procedure was performed according to the manufacturer protocol except for an additional 1 min centrifugation after the last washing step to completely remove eventual ethanol leftover ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from transduced primary cortical neurons or brain tissue from transduced animals was extracted using Quick-DNA Miniprep Kit (Zymo Research, USA) following manufacturer recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... We extracted DNA from the whole fly or from just the body (saving the heads for immunostaining from a subset of flies) using Quick-gDNA miniprep kit (Zymo Research R1050). We designed primers upstream and downstream of the Tra binding sites as follows:
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was then extracted from each sample using the Quick-RNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, CA). The total amount of RNA in each sample was subsequently quantified with a Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Systems Biology 2023Quote: DNA was extracted from approximately 100 mg of each gut content sample using the ZymoBIOMICS™ 96 MagBead DNA Kit (D4308) (Zymo Research), following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... four independent differentiations from C71G+/- iMGs and three independent differentiations from M114T+/- and M114T+/+ iMGs were lysed with Trizol and total RNA was isolated with Direct-zol RNA Microprep Kit (Zymo Research, R2060) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then washed with PBS and lysed in Tri-reagent and RNA was extracted using the Direct-zol RNA miniprep kit (Zymo Research, # R2050) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 38% v/v saturated acidic phenol) and the Direct-zol RNA MiniPrep Kit (including on-column DNase I treatment, Zymo Research, R2052) or according to the protocol for TRIzol™ Reagent (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from agar plugs (as described above for PFGE chromosome preparation) using the Zymoclean gel DNA recovery kit (Zymo Research, D4001T) and successively digested with Exonuclease V (RecBCD ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleaned pools of samples were gel purified to remove excess primer and eluted cDNA was cleaned through Zymo DNA Clean and Concentrator kit (Zymo Research #D4014).
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bioRxiv - Biochemistry 2023Quote: ... equal amount of nuclease free water was added to each sample and purified using Oligo Clean & Concentrator kit (Zymo Research, CAT#: D4061). The samples were eluted in 6 μL of nuclease free water The samples were eluted in 6 μL of nuclease free water and an equal amount of 2x denaturing loading buffer was added for separation on a 20% denaturing polyacrylamide gel as described above ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Immunology 2023Quote: ... we have isolated DNA for preparing Reduced Representation Bisulfite Sequencing (RRBS) libraries using Zymo-Seq RRBS Library Kit (Zymo Research, USA, #D5461). In brief ...
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bioRxiv - Bioengineering 2023Quote: ... and homogenized further using the mixer mill MM400 (Retsch) followed by RNA isolation with the Direct-zol™ DNA/RNA Miniprep Kit (Zymo Research). Total RNA concentrations were measured using the NanodropTM 2000c spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... The transcribed product was treated with DNase I for 30 min at 37°C and then purified using RNA Clean and Concentrator Kit (Zymo Research #R1016).
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were grown in Sc-Ura medium for 1 d and plasmid DNA was purified using Zymoprep II Yeast plasmid Miniprep kit (Zymo Research, CA). Plasmids were then transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR amplified DNAs were purified by DNA Clean and Concentrator or Zymoclean™ Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Primers were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-S-UTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research, R1013) with in-column DNAse reaction to eliminate traces of genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... was added to each well followed by isolation of total RNA using Direct-Zol RNA Mini-Prep Plus Kit (Zymo Research, R1058) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The crosslinking was reversed at 65 °C and the samples were purified using the ChIP DNA Clean and Concentrator kit (Zymo Research, D5205). The DynaMagTM-2 Magnet (Thermo Fisher Scientific ...