Labshake search
Citations for Zymo Research :
6101 - 6150 of 6511 citations for Rat Golgi phosphoprotein 3 GOLPH3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Sodium bisulfite conversion of 500 ng of genomic DNA from each subject was performed using the EZ DNA Methylation-Gold™ Kit (Zymo, USA). DNA methylation levels at specific genomic sites were investigated by targeted deep bisulfite sequencing following the protocol by Moser et al ...
-
bioRxiv - Molecular Biology 2022Quote: ... The digested codon random mRFP with singlestranded overhangs was cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). The complete 15 μl containing the codon random mRFP library was used in a ligation reaction to generate the plasmid library.
-
bioRxiv - Molecular Biology 2022Quote: ... The ligation mixture was cleaned and concentrated to 10 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). 1 μl of the codon randomised mRFP library was transformed into electrocompetent DH10B cells (20 μl competent cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... The linear plasmid was excised from the gel and purified to 30 μl mQ using the Zymoclean™ Gel DNA Recovery Kit by Zymo (D4002). The codon random mRFP library (15 μl ...
-
bioRxiv - Systems Biology 2022Quote: ... Total DNA extractions were performed for tea broth and pellicle samples using Quick-DNA Fecal/soil Microbe Miniprep kit (ZYMO Research Corp.) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... insect legs were mechanically crushed and resuspended in 50 μl of sterile water and the genomic DNA (gDNA) was extracted using a Quick-DNA Universal kit (Zymo research, USA). 2 μl of the gDNA were added to a GoTaq® Green Master Mix (Promega ...
-
bioRxiv - Genomics 2022Quote: ... 500ng of high-quality genomic DNA was bisulfite converted using the Zymo EZ-96 DNA Methylation kit (Zymo Research, Irine, CA, USA). Bisulfite converted samples were then amplified ...
-
bioRxiv - Molecular Biology 2022Quote: ... The dsDNA blocks were excised from the gel and purified to 20 μl mQ using the Zymoclean™ Gel DNA Recovery Kit (Zymo, D4002). 5 μl of the dsDNA was used to quantify the DNA concentration with the Qubit assay (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
-
bioRxiv - Molecular Biology 2022Quote: ... The fulllength assembled product was excised from the gel and purified to 44 μl mQ using the Zymoclean™ Gel DNA Recovery Kit by Zymo (D4002). 10 units of SapI (NEB ...
-
bioRxiv - Microbiology 2022Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... epithelial sheets and hyphae pellets were grinded in liquid N2 and processed as described before (Amorim-Vaz et al., 2015a) combining two phase separations and DirectZol™ RNA MiniPrep kit (Zymo Research). DNase treatment was performed off-column using a DNA-free™ Kit (Life Technologies) ...
-
bioRxiv - Neuroscience 2021Quote: ... cultured neurons at 15 DIV were left untreated or treated for 48 h with 2 μM TTX and then processed for total RNA extraction using the kit Direct-zol™ RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from the whole rosette leaves of 32 day-old control and drought-stressed plants using the Quick-RNA Miniprep Kit (Zymo-Research, USA). RNA quality and quantity were determined using a Nabi UV/Vis Nano Spectrophotometer (LTF Labortechnik ...
-
bioRxiv - Cancer Biology 2020Quote: ... RNA was isolated from tumor cell populations and unstained whole tumor single cell suspensions using the Quick-RNA™ Microprep Kit (Zymo R1050) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Tissue RNA extraction was performed using the Direct-zol RNA miniprep plus kit with DNAase treatment per package insert (Zymo, Irvine, California). SIV and CD4 primers for RT PCR and amplification conditions were as previously published [28] ...
-
bioRxiv - Physiology 2022Quote: Total RNA from adipose tissues (experiment 1) was extracted using the Direct-zol™ RNA MiniPrep Plus kit protocol (Zymo research, #R2071). RNA was reverse-transcribed using the SuperScript™ III Reverse Transcriptase Kit (ThermoFisher ...
-
bioRxiv - Developmental Biology 2022Quote: RNA was extracted from flash-frozen fetal liver (left lobe) samples using a Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA)) per manufacturer instructions ...
-
bioRxiv - Cell Biology 2022Quote: RNA isolation of digested samples was performed using the Directzol RNA Miniprep kit with DNAse-I treatment to remove trace DNA before RNA elution (Zymo Research, R2050). RNA was quantified via Nanodrop spectrophotometry ...
-
bioRxiv - Biochemistry 2022Quote: ... the LentiCRISPRv2 hygro plasmid was purified using a 0.7% agarose gel and a Zymoclean gel DNA recovery kit (Zymo Research cat # D4001). The prepared sgRNA oligo duplexes were then ligated into the purified LentiCRISPRv2 hygro plasmid ...
-
bioRxiv - Neuroscience 2022Quote: ... total cellular RNA using ∼1×106 cells from one well of a six-well plate was isolated using TRIzol reagent followed by Direct-zol RNA MiniPrep Plus kit purification (Zymo Research # R2070). RNA was quantified using Qubit RNA HS Assay kit and cDNA synthesised from ∼2µg of RNA using SuperScript IV VILO Master Mix kit following gDNA removal with ezDNase ...
-
bioRxiv - Cancer Biology 2019Quote: ... genomic DNA (250-500ng) was modified by sodium bisulfite reaction using the EZ DNA Methylation-Lightning kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... Small RNAs were sized selected and purified from a 15% denaturing polyacrylamide-urea gel using the ZR small-RNA™ PAGE Recovery Kit (Zymo Research). For oxidized RNA library preparations ...
-
bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted and genomic DNA was removed using the Direct-zol 96-well MagBead RNA Kit (Zymo Research, Orange, CA). cDNA was synthesized from total RNA using the iScript™ Reverse Transcription System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... All samples were equilibrated to a total volume of 50 μL and then DNA was extracted using Zymo Quick-DNA Microprep Kit (Zymo Research, CA). Procedure was performed using modifications to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... were thoroughly mixed 1:1 with 2X DNA/RNA Shield™ and incubated at RT for 20 min for inactivation and vRNA was extracted using the Quick-RNA™ Viral Kit (Zymo Research) following the manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2019Quote: ... for 1 h at 37°C to remove free oligonucleotide and purified by ethanol precipitation/ or the DNA Clean and Concentrator 5 kit (Zymo Research, D4003T). Libraries were resolved on a 2% MetaPhor agarose (Lonza ...
-
bioRxiv - Plant Biology 2019Quote: Total RNA from 4 weeks old Arabidopsis thaliana WT Col-0 and mta mutant plants was isolated using Direct-zol™ RNA kit (Zymo Research). RNA was quantified by Qubit RNA Assay Kit (Life Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
-
bioRxiv - Genomics 2020Quote: ... and 500ng of DNA from each sample was bisulfite converted using the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA USA) following the manufacturer’s protocol using the specified modifications for the Illumina Infinium Methylation Assay ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were verified by Sanger sequencing and purified with the endo-free ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, USA). Two micrograms of plasmid DNAs were used to transfect small-scale HEKT293 cultures and optimize expression conditions both via mRuby2 visualization and western blot analysis ...
-
bioRxiv - Developmental Biology 2019Quote: Total RNAs were extracted from freshly isolated (micro-dissected) SVZ tissue or hippocampi using Direct-zol™ RNA MiniPrep kit (Zymo Research). Some RNAs were reverse transcribed with SuperScript III (Invitrogen) ...
-
bioRxiv - Genomics 2019Quote: ... NGS libraries consisted of tagmented DNA reisolated from excised regions of gel lanes using a Zymoclean Gel DNA Recovery® kit (Zymo Laboratories) (13).
-
bioRxiv - Microbiology 2019Quote: ... The samples were preserved in the field immediately after sampling using an Xpedition Soil/Fecal DNA MiniPrep kit (Zymo Research, Irvine, CA) frozen ...
-
bioRxiv - Microbiology 2021Quote: ... The fungal isolates were identified by extracting genomic DNA with the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research; Irvine (Fig, CA), amplifying and sequencing the internal transcribed spacer (ITS ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: DNA was extracted from 100 mg caecum content using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA, USA) with minor adaptations from manufacturer’s instructions (Ducarmon et al ...
-
bioRxiv - Physiology 2021Quote: ... Total RNA was extracted from each supernatant with the Zymo Quick-DNA/RNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s protocol for tissue samples ...
-
bioRxiv - Plant Biology 2021Quote: ... The purified adapter-added genomic DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold kit (Zymo Research, Cat#: D5005). Then the treated genomic DNA was resolved in a 1.5% agarose gel ...
-
bioRxiv - Bioengineering 2020Quote: ... Single-fly genomic DNA preps were prepared using the solid tissue protocol of the Quick-DNA™ Miniprep Plus Kit (Zymo Research). In total ...
-
bioRxiv - Genomics 2019Quote: ... followed by bisulfite conversion of purified adapter-ligated DNA using an EZ DNA Methylation-Gold Kit™ (ZYMO Research, Irvine, CA, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... coli cells were recovered by centrifugation and pGFP was purified using ZymoPURE™ Plasmid Maxiprep Kit (catalog #D4203, Zymo Research, Irvine, USA). The DNA concentration was measured in a NanoDrop™ 2000/2000c Spectrophotometer (ThermoScientific® ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Genomic DNA (1 μg) was treated with sodium bisulfite using the EZ DNA Methylation Gold Kit per manufacturer’s instructions (Zymo Research; Irvina, CA). Bisulfite converted DNA (∼20 ng ...
-
bioRxiv - Bioengineering 2021Quote: ... control targeted cells (cells that underwent CRISPR/Cas9-mediated targeting and HDR of an expression cassette at a non-GSH locus) and two independent cultures of untargeted cells using Direct-zolTm RNA Miniprep kit (Zymo Research, ZYR.R2052). 1μg of RNA was converted into cDNA with Superscript IV Vilo MM (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: We extracted RNA and DNA from tissue biopsies using the ZR-Duet DNA/RNA MiniPrep Plus kit (Zymo Research, catalog number D7003). We performed the in-column DNase I treatment as specified in the kit to remove residual DNA from the RNA fraction ...
-
bioRxiv - Biophysics 2021Quote: ... RNA was then extracted from the aqueous phase using a spin column-based purification kit (RNA Clean & Concentrator-5, Zymo Research # R1015) and reverse-transcribed into cDNA using SuperScript VILO Master Mix (Thermo Fisher # 11755050) ...
-
A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was extracted and genomic DNA was removed using the Direct-zol MagBead RNA Kit and following manufacturer’s protocol (Zymo Research, Orange, CA). RNA concentrations and contamination were determined spectrophotometrically using a Nanodrop (ND-1000 ...
-
bioRxiv - Microbiology 2021Quote: Six PCR reactions from each sample were pooled and purified with the DNA Clean & Concentrator™ kit (Zymo Research, Irvine, CA, USA), and quantified with Qubit 1.0 fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2019Quote: ... #10296010) were added to the flow-through and RNA was extracted using the Direct-zol RNA Mini-Prep kit (Zymo Research #R2052) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The RNA was purified and treated with DNase by using the Direct-zol RNA MiniPrep kit (Zymo Research Corporation, Orange, CA, USA), according to the manufacturer’s recommendation ...