Labshake search
Citations for Zymo Research :
5801 - 5850 of 6483 citations for Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Viral RNA in supernatants of infected cells and intracellular RNA were extracted using Quick-RNA™ Viral 96 Kit (Zymo Research). RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from primary neurons was extracted using home-made TRIzol reagent in combination with Direct-zol RNA Kit (Zymo Research). The samples were fixed in cold methanol for 10 mins at −20C ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted from larval fat bodies (2-5 pooled/sample) or larval brains (5 pooled/sample) using the Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Plant Biology 2023Quote: Rossette leaves of about one-month-old plants were collected for RNA extraction with Zymo Direct-Zol RNA miniprep Kit (Zymo Research). 1 ug of RNA were used for cDNA synthesis with iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... 500 ng of genomic DNA from each sample was bisulfite-converted with the EZ DNA Methylation™ Kit (Zymo Research, D5002) and analyzed using Infinium HumanMethylationEPICBeadChip™ Kit (Illumina ...
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bioRxiv - Genetics 2022Quote: ... Leukocyte genomic DNA (500 ng) was treated with bisulfite using the DNA EZ Methylation-Gold methylation kit (Zymo Research, CA, USA), following the recommendations stipulated by the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from the heads of 25-30 adult male flies using TRIzol Reagent and Direct-zol RNA microprep kit (Zymo Research) followed by cDNA preparation from 0.5–1 μg total RNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
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bioRxiv - Neuroscience 2022Quote: ... ATAC-seq libraries from young and old cells were amplified with 11-14 PCR cycles and then purified using the Zymo DNA Clean & Concentrator kit (Zymo, D4014) and eluted in 15 μL of nuclease-free H2O ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was purified from all input and output library cultures using the Quick-DNA™ Miniprep Plus Kit (Zymo Research), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR products were then size selected to remove adaptor-dimers below 200 bp using three subsequent size-selection rounds with a Select-a-Size DNA Clean & Concentrator Kit (D4080, Zymo Research). Finally ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL each reaction was transformed into 100 μL DH10B and transformed according to the Mix and Go Transformation kit (Zymo Research).
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bioRxiv - Plant Biology 2022Quote: ... genomic DNA was extracted from leaf tissue of ASF1B-ZF T2 with a CTAB-based method and converted using the EZ DNA Methylation-Lighting kit (ZYMO research). We amplified the methylated region of the FWA promoter region and several control regions with primers described previously48 ...
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bioRxiv - Neuroscience 2022Quote: ... Bisulphite conversion was then carried out with 750 ng of DNA using the EZ DNA Methylation Kit (Zymo Research, Irvine, USA), followed by genome-wide methylation profiling using the Infinium HumanMethylationEPIC Bead Chip (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: The cells were lysed in TRIzol (Thermo Fischer Scientific) and the total RNA was extracted with Direct-zol RNA extraction kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in 50-100 mL of LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE II Plasmid Midiprep Kit (Zymo #D4201) by following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE Plasmid Miniprep Kit (Zymo #D4210) by following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Synthetic Biology 2024Quote: ... Donor DNA was generated by PCR amplification from the pre-assembled donor DNA plasmids and purified by DNA Clean & Concentrator Kit (Zymo Research).
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bioRxiv - Evolutionary Biology 2024Quote: We used a Trizol-based protocol to extract RNA from primate samples using the Direct-zol RNA Microprep kit (R2060, Zymo Research). All steps were centrifuged at 13,000 g for 30 s unless otherwise noted ...
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bioRxiv - Plant Biology 2024Quote: ... was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research). Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Plant Biology 2024Quote: ... A 200 mg soil sample from each falcon tube was taken for DNA extraction using the Zymo Quick DNA Fecal/Soil Microbe MiniPrep Kit (Zymo Research). Before sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... which were removed from the mutational libraries by gel purification using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Purified products were again transformed into either NEB 10-beta (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research; Tustin, CA). Eluted RNA was analyzed in a Nanodrop Lite (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... S4) and cells were collected by filtration for RNA purification using the RNA Clean and Concentrator kit (Zymo Research, Irvine CA). rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre ...
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bioRxiv - Cell Biology 2024Quote: ... Assembled plasmids (termed pTREP-ID vector hereafter) were then purified using a DNA Clean and Concentrator purification kit (Zymo, no. D4014) and transformed into One Shot ccdB Survival 2 T1R resistant competent cells (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... This was done by isolating genomic DNA of each yeast strain using the Zymoprep yeast plasmid miniprep II kit (Zymo Research) according to the manufacturer’s protocol and PCR amplifying the region of interest.
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bioRxiv - Synthetic Biology 2024Quote: ... colonies were scraped with 50 mL 2XYT and plasmid DNA extracted with the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203). These plasmid libraries constitute the “post-selection libraries.”
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bioRxiv - Cancer Biology 2023Quote: ... Modifications to the published method are the following: 1) extraction of RNA prior to cDNA reverse transcription with the Zymo Direct-zol-96 RNA isolation kit (Zymo, #R0256) 2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... were transformed into Saccharomyces cerevisiae VL6-48(Yamanaka et al., 2014) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). The correct promoter insertion was screened by PCR using primers upstream and downstream of the target site (Table S4) ...
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bioRxiv - Microbiology 2023Quote: ... were grown at 28°C to stationary phase and cell pellets from 5 mL cultures were collected for total RNA extraction using a Direct-zol RNA miniprep kit (Zymo Research). 10 μg of isolated total RNA was subjected to DNaseI treatment with 20 units of Turbo DNaseI (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and PPL554 of bacterial cultures grown overnight in NB media overnight was extracted using the QuickDNA™ Fungal/Bacterial Miniprep Kit sourced from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: Stored bacterial pellets were used for RNA extraction using the Quick-RNA Fungal/Bacterial Miniprep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... The total RNA of isolated renal tubules was immediately extracted using Quick-RNA™ MicroPrep kit (Zymo Research, Irvine, CA, USA). Reverse transcription was performed using Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 50 mg of finely minced tissue in all cases using the Quick-RNA™ Plant Miniprep kit from ZYMO RESEARCH® (ref ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using protocols from the Quick-RNA Fecal/Soil Microbe Microprep Kit with some modifications (Zymo, Irvine, California, USA). RNA samples were initially preserved with 1 mL of activated sludge (i.e. ...
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bioRxiv - Microbiology 2024Quote: ... To determine the amount of virus that bound the cells viral RNA was isolated with RxnDirect-zol RNA Miniprep Plus kit (Zymo Research) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2024Quote: The infected cells or homogenized lungs were lysed in Trizol to prepare total RNAs using Direct-Zol RNA Microprep kit (Zymo Research) with DNase treatment according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The vector and PCR products were digested with restriction enzymes and band-purified (vector) or cleaned (PCR products) using the Zymoclean Gel DNA Recovery Kit (Zymo Research) or the DNA Clean & Concentrator-5 kit (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA for long read sequencing was extracted from our Salmonella enterica serovar Typhimurium strain 14028 isolate using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Library preparation was performed using the Genomic DNA by Ligation (SQK-LSK 109 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was purified from bilateral NAc core and shell separately using the Direct-zol RNA kit following the manufacturer’s instructions (Zymo Research, CA), including DNase to remove genomic DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from samples collected at the 48h timepoint (72h after the transfection) using Quick-DNA/RNATM Microprep Plus Kit (Zymo Research) including the DNAse I step ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from corals at the 48h timepoint (72h after the transfection) using Quick-DNA/RNATM Microprep Plus Kit (Zymo Research). The level of 8-Hydroxy-2’-deoxyguanosine (8-OhdG ...
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bioRxiv - Genomics 2024Quote: ... Purified genomic DNA from undifferentiated and differentiated adipocytes was subjected to bisulfite modification using the EZ DNA Methylation kit (Zymo Research). Approximately 0.5-1μg of genomic DNA was treated with bisulfite and eluted in 20μl elution buffer following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The bacmid encoding Halo-Ku80/Ku70 complex was obtained by electroporating pAM086 into DH10EMBacY electro-competent cells and purified using ZR BAC DNA miniprep kit (Zymo Research).
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bioRxiv - Microbiology 2024Quote: ... and the total RNA of each sample was isolated with the Direct-zolTM RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: For short read Illumina sequencing DNA extractions were carried out with the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, UK), with the addition for S ...
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bioRxiv - Molecular Biology 2024Quote: RNA purification and on-column DNase I treatment were performed using a Quick-RNA Fungal/Bacterial Miniprep kit and DNase I set (Zymo Research) following the manufacturer’s recommendations with minor modifications ...