Labshake search
Citations for Zymo Research :
5751 - 5800 of 6579 citations for Hemoglobin Colorimetric Detection Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from Vero cells after 4 days post infection using the Direct-zol RNA isolation kit (Zymo Research). 1 μg of RNA isolate was subjected to rRNA removal with the NEBNext rRNA Depletion Kit (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... The ITS regions were amplified by qPCR using ITS-specific primers provided by the Femto(tm) Fungal DNA Quantification Kit (Zymo Research) to quantify the relative abundance of fungal DNA following the manufacturer’s recommendations for the three biological replicates ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from each collected fraction using the RNA Clean and Concentrator-25 kit following the manufacture’s instruction (Zymo Research, R1017). Reverse transcription and qPCR were performed as described above ...
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bioRxiv - Microbiology 2021Quote: Viral particles were collected from virus-infected A549 hACE2 cell supernatants at 72 hours post infection (100 μL) and viral RNAs were isolated from the supernatants using the Quick RNA Viral 96 Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were prepared and transformed with the Y2H plasmid constructs by using the Frozen-EZ Yeast Transformation II kit (Zymo Research, USA) and following the manufacturer-supplied protocol and recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The extracted RNA was treated with DNase and cleaned up using the RNA Clean & Concentrator-25 Kit (Zymo Research, Freiburg, Germany). RNA samples were then used for sequencing on an Illumina Hiseq2000 platform ...
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bioRxiv - Genomics 2021Quote: ... DNA from the ChIP and input control samples was isolated using ChIP DNA Clean and Concentrator Kit (Zymo Research, Irvine, CA) to prepare sequencing libraries using NEBnext Ultra II kit (NEB) ...
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bioRxiv - Genomics 2021Quote: ... The libraries were pooled based on qPCR data and subjected to bisulfite conversion using the EZ DNA Methylation Direct Kit (Zymo Research) with changes to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Total DNA was purified from collected feces using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, Canada, USA) according to the manufacturer’s instruction and subjected to shotgun sequencing ...
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bioRxiv - Microbiology 2020Quote: Dim and lit cells were sorted into 500 µl TriZol and RNA was extracted with DirectZol RNA purification kit (Zymo Research) with the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The yeast DNA samples were collected by thawing frozen yeast and following the standard procedure of a Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... and DNA extraction of 2017 surface precipitate samples was extracted using the ZymoBIOMICS™ DNA/RNA Mini Kit (Zymo Research Corp.). All extractions were performed according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: Hemolymph samples (n=112) were centrifuged for 10 minutes at 14000 g with RNA extracted using Quick DNA/RNA Microprep Plus Kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted from 1 ml breastmilk using Quick-DNA Fungal/Bacterial extraction kit following the manufacturer’s instructions (Zymo Research, USA). Extraction kits were purchased separately for Batch 1 and Batch 2 ...
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bioRxiv - Microbiology 2020Quote: ... and from bacteria harvested from broth cultures were extracted with ZymoBIOMICS™ DNA Miniprep or Microprep kits (Zymo Research, Irvine, CA). Freshly-dissected ...
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bioRxiv - Microbiology 2019Quote: ... 5 µL of ligated plasmid was then transformed into competent BW23473 cells (made using the Mix & Go E. coli Transformation Kit & Buffer Set, Zymo Research) and plated on LB + amp plates and grown overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Duplexed 12RSS4-9 was digested with MluI and EcoRI and purified using the Zymo DNA Clean and Concentrator Kit (Zymo # D4013). The double-digested and gel purified pMX-INV and duplexed 12RSS4-9 were ligated at a 3:1 12RSS4-9:pMX-INV ratio with T4 DNA Ligase by overnight incubation at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The reactions were treated with DNAse (15 min at 37°C) and remaining RNA was recovered using Direct-zol RNA mini prep kit (Zymo research) according to the protocol ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from cultured cells using BIO TRI RNA reagent (Bio-lab) and Direct-zol RNA MiniPrep kit (Zymo research), as instructed by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions yielding single amplicons as judged by agarose gel electrophoresis were purified using a PCR clean up kit (Zymo Research) and sent for DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... About 300-500 bp DNA were selected using agarose gel electrophoresis and were purified by the Zymoclean gel DNA recovery kit (Zymo Research). High Sensitivity DNA Chips of Agilent Bioanalyzer 2100 were utilized to check library quality ...
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bioRxiv - Genomics 2021Quote: ... Clones were verified by Sanger sequencing (Quintara Biosciences) prior to retransformation and maxiprep using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Genetics 2019Quote: Four 7 micron sections of the left ventricle were used to isolate total RNA using the Quick-RNATM FFPE kit (Zymo Research). 1 µg of total RNA was used to generate cDNAs using the SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from OSC:eFH-piwi and wild type (WT) OSC using Trizol and Direct-zol RNA MiniPrep kit (Zymo Research, R2051). Complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then cultured for 6 days prior to RNA isolation using the ZR-Duet DNA/RNA MiniPrep Plus Kit (Zymo D7003). RNA quality and concentration were confirmed on an Agilent TapeStation and Life Technologies Qubit ...
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bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
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bioRxiv - Immunology 2022Quote: ... Plasmid DNA was extracted from ten million yeast from each round of selection using the Zymoprep Yeast Miniprep Kit (Zymo Research), following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
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bioRxiv - Cell Biology 2022Quote: Testing and control combinations of plasmids were co-transformed into yeast AH109 strain using the Frozen-EZ Yeast Transformation II Kit (ZYMO, T2001) and cultured on a SD/-Leu-Trp selective plate at 30 °C for 2–4 days ...
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bioRxiv - Biochemistry 2022Quote: ... which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research, Irvine, CA). The Bxb1 recombinase expression vector (pCAG-NLS-HA Bxb1 ...
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bioRxiv - Cell Biology 2022Quote: ... Cellular RNA was isolated by either phenol/chloroform extraction or using the Direct-zol RNA Microprep kit (Zymo Research, Irvine, CA). RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... All the steps from here were optimized from the standard protocol provided with the EZ Nucleosomal DNA Prep Kit (D5220, Zymo Research). Briefly ...
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bioRxiv - Plant Biology 2022Quote: Extraction of total RNA from Arabidopsis seedlings and Marchantia spores was carried out using the Direct-Zol RNA MINIprep kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: The 2 final 15kb fragments were gel extracted via agarose gel electrophoresis and purified with the Large Fragment DNA Recovery Kit (Zymo Research) and eluted in water ...
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bioRxiv - Microbiology 2022Quote: ... The cells and medium were then collected and subjected to RNA extraction using the Direct-zol RNA MiniPrep kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The remaining PCR reaction was run with the determined cycle number with the same cycler conditions as before and DNA was purified with Zymo ChIP clean and concentrator kit (Zymo research) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: DNA was obtained from at least 107 yeast per round of selection using a Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). Primers were designed to amplify the Aga2 leader peptide through to the beginning of the β2M sequence ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with sterile PBS and total RNA was isolated using the Direct-Zol Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA sequencing libraries were prepared by amplifying and barcoding the V1-V2 region of the 16S rRNA gene using the Quick-16S NGS Library Prep Kit (Zymo Research). Samples were pooled and sequenced on the Illumina MiSeq platform with 2 × 250 base pair standard run at Washington University DNA Sequencing Innovation Lab.
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bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR product was run on a 1% Agarose gel and the band at the appropriate size was excised and the DNA extracted using the Zymoclean Gel DNA Recovery kit (Zymo Research). Purified insert DNA was cloned into the linearized modRNA plasmid (5MCS3)(K et al. ...
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bioRxiv - Bioengineering 2022Quote: ... The bands of interest were cut from the denaturing gels and purified using ZR small-RNA PAGE Recovery Kit (Zymo Research). The purified RNA samples were stored in nuclease free water at -20 °C.
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bioRxiv - Immunology 2022Quote: ... Cells were sorted into TRIzol (Thermo-Fisher) and RNA was isolated using a Direct-zol RNA-mini kit with RNA clean and concentrator columns (Zymo Research) and treated with DNase (Qiagen).
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bioRxiv - Cancer Biology 2022Quote: ... RNA from either CD34+ cells sorted after transduction or bulk cells at day 12 of culture was extracted using Direct-Zol RNA MicroPrep Kit (Zymo Research) and reverse transcription was performed with SuperScript Vilo cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... An equal volume of 100% ethanol (800uL) was added immediately and mixed well before proceeding to RNA isolation with the Direct-zol RNA Microprep Kit (Zymo Research). DNase treatment and RNA isolation proceeded according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were made from the Zymo mock community using the LoopSeq 16S long-read kit (referred to throughout as ‘Zymo-Loop’). This preparation was also followed for the ATCC-gut and ATCC-oral communities as well as the uterine lavage and cyanobacterial enrichment samples ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA precipitants were treated with 100 μl of 0.04 U Turbo DNase I at 25°C for 30 min to remove DNA contamination and cleaned using an RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Research, USA). The RNA was eluted in 16 μl RNase-free water for subsequent experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested in TRIzolTM LS reagent (Invitogen) and total RNA was extracted using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instruction ...
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bioRxiv - Microbiology 2021Quote: ... DNA with a size of at least ~200 bases was excised and purified using a gel DNA recovery kit (Zymo Research) with elution in 50 μl of nuclease-free water ...