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Citations for Zymo Research :
5701 - 5750 of 6585 citations for Rat Nuclear Receptor Subfamily 4 Group A Member 2 NR4A2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Molecular Biology 2022Quote: ... the reaction was stopped by adding MN Stop Buffer and the nucleosomal DNA isolation was carried out according to the kit protocol (D5220, Zymo Research). Illumina TruSeq PCR free DNA library was prepared without bead-based size selection followed by a preparative size selection on the PippinHT to remove non- ligated adaptors and library molecules with inserts >500 bp.
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Molecular Biology 2022Quote: ... We followed the PCR with an agarose gel extraction and a column purification using the Oligo Clean & Concentrator kit (Zymo Research). The DNA template was then in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were homogenized in RNA STAT-60 (Tel-Test, Inc., Friendswood, TX) and total RNA purified using a Direct-zol RNA miniprep kit (Zymo Research). We used an RNA Clean and Concentrator Kit (Zymo Research ...
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bioRxiv - Neuroscience 2021Quote: ... we used Q5 hot-start high-fidelity DNA polymerase to amplify the transcripts of each candidate gene from complementary DNA (cDNA) synthesized using the total RNA of w1118 fly heads (extracted using MiniPrep kit; Zymo Research). For each candidate gene ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting product was then treated with Turbo DNase and the remaining RNA was purified with the Zymo RCC-25 column purification-25 kit (Zymo Research). The A/B RNA standards were quantified using Qubit RNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and fl/fl from each nest/uterus) that were subsequently cleaned and DNAse treated using the zymo-clean&concentrator kit (Zymo Research). For primary neurons ...
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bioRxiv - Molecular Biology 2021Quote: ... The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350 bp) was cut and purified with the Zymoclean Gel DNA Recovery Kit (Zymo #D4008). We note that size selection for DNA larger than 200 bp greatly reduces the ratio of unligated monomers in Micro-C data.
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bioRxiv - Molecular Biology 2021Quote: ... 10 fully grown GV oocytes (an equivalent of 40 haploid genomes and 80 single-stranded DNAs after bisulfite conversion) were directly subjected to EZ DNA Methylation-Direct kit (Zymo Research) for bisulfite conversion with the following modifications ...
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bioRxiv - Molecular Biology 2021Quote: High-molecular-weight genomic DNA from HAP1 Δ72 and HepG2 Δ15 deletion clone was extracted using Quick-DNA kit (Zymo Research) and shipped to Samplix Services (Denmark ...
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bioRxiv - Bioengineering 2022Quote: ... cerevisiae strain EBY100 (ATCC MYA-4941) (Boder & Wittrup, 1997) using frozen-EZ yeast transformation II kit (Zymo Research, Cat. No. T2001) and grown on SD-CAA agar plates for 3 to 4 days ...
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bioRxiv - Cell Biology 2022Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
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bioRxiv - Genomics 2020Quote: ... DNA was then extracted from approximately 1 ml of the red blood cell pellets using Zymo Bead Genomic DNA kit (Zymo Research) following the manufacturer’s procedures ...
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bioRxiv - Genomics 2020Quote: ... 5 µl reactions were purified with the ZR-96 Oligo Clean & Concentrator and 15 µl reactions were purified with the Oligo Clean & Concentrator kit (Zymo Research) and eluted with probe resuspension buffer ...
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bioRxiv - Genomics 2020Quote: ... The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350bp) was cut and purified with Zymoclean Gel DNA Recovery Kit (Zymo #D4008). We note that the size-selection for DNA larger than 200bp greatly reduces the ratio of unligated monomer in Micro-C data.
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bioRxiv - Developmental Biology 2020Quote: ... Samples were re-suspended with 50 μL of nuclease-free water and subsequently purified using the RNA Clean & Concentrator kit (Zymo, R1018). The concentration of each sample was determined using a Nanodrop spectrophotometer ...
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bioRxiv - Genomics 2020Quote: ... the adaptor-ligated MspI fragments were bisulfite-treated for two consecutive rounds using Zymo’s EZ DNA Methylation-Gold Kit (Zymo cat. D5005) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The reverse cross-linked samples were treated with 20 μg/ml Proteinase K and purified using ChIP DNA Clean and Concentrator kit (Zymo research). The three reactions per condition were pooled at this stage ...
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bioRxiv - Molecular Biology 2021Quote: ... one each with the insert in genomic and in antigenomic orientation for plasmid stock preparation using ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Cell Biology 2019Quote: The presence of the point mutations in the genome of the clones was confirmed by extraction of genomic DNA using Quick-DNATM Universal Kit (ZYMO Research) followed by amplification of the N-terminal of BRCA2 (aa 1-267 ...
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bioRxiv - Cell Biology 2019Quote: ... Genotyping PCR was performed using Taq polymerase on genomic DNA extracted from SMB55 cells using ZymoBead™ Genomic DNA kit (Zymo). Genomic DNA extracted from Ptch1-/- mouse embryonic fibroblasts and a wild-type mouse were used as a control for the mutant and wild-type alleles respectively ...
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bioRxiv - Neuroscience 2019Quote: ... The gDNA of the sorted cells (both N2A and NSCs) was extracted with the Genomic DNA(tm) - Tissue MiniPrep kit (Zymo Research) following the protocol of the manufacturer for cell suspensions ...
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bioRxiv - Microbiology 2019Quote: Genomic extraction was preformed on 105 – 106 cells from the in vitro evolution experiments using the Quick-DNA Miniprep Plus Kit (Zymo Research). The gDNA was then diluted to a range between 100-200ng/5μl in TE buffer ...
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bioRxiv - Cancer Biology 2019Quote: Tumor-derived gDNA was purified and concentrated using the genomic DNA Clean and Concentrator-10 (gDCC-10) kit following manufacturer instructions (Zymo Research); concentration was assessed by Qubit and quality was assessed by Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the total RNA from adults was prepared with Direct-Zol RNA miniprep kit with on-column DNase treatment (ZYMO Research, #2050). cDNA synthesis and qPCR were carried out as described above ...
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bioRxiv - Microbiology 2019Quote: ... The assembled DNA was then cleaned and concentrated using Zymo Clean and Concentrator-5 kit (SKU D4013 Zymo Research, Irvine, CA), quantified by Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: 500 ng of genomic DNA per sample was used for bisulfite conversion with the EZ DNA Methylation™ Kit (Zymo Research) according to the manufacturer’s instruction ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: DNA extraction from clonal iPSC lines cultured in 96-well plates was performed using the Quick-DNA 96 Kit (Zymo Research). Briefly ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA from BMDM cell lines or tissues was isolated using the Direct-zol™ RNA MiniPrep Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The pellet was resuspended in an equal volume of TRIzol reagent and then purified using the Direct-zol RNA MiniPrep kit (Zymo Research). All RNA preparations were treated with DNase I Amplification Grade or Turbo DNA-free (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... Total RNA was isolated from cell pellets in 5 batches (each batch contained one of each of the 5 strains) using the ZR Fungal/Bacterial RNA mini-prep kit including the DNase I on-column digestion step (Zymo Research). Each cell pellet was resuspended in lysis buffer and transferred to screw-capped tubes containing glass beads and the cells were broken open using a mini-beadbeater (Biospec Products ...
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bioRxiv - Genomics 2019Quote: ... The final NET-seq library running at ∼150 bp was extracted and further purified using the ZymoClean Gel DNA recovery kit (Zymo Research). Sample purity and concentration was assessed in a 2200 TapeStation and further sequenced on a HiSeq 2500 Illumina Platform (Supplementary Table 2).
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bioRxiv - Genomics 2020Quote: Total RNA from testicular tissue samples of the four bulls was extracted using Direct-zol RNA Mini Prep Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples from the replication cohort DNAs were submitted for bisulfite conversion using 1ug of DNA in the e EZ-96 Methylation Kit (Zymo Research). Converted DNA was used as a template for bisulfite-specific PCR with the HotStartTaq Plus (QIAGen ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Synthetic Biology 2019Quote: Genomic DNA was prepared from both the WT strain as well as the different melamine-utilizing strains by using the Quick-DNA Fungal/Bacterial Kit (Zymo Research). Library preparation was performed according to Illumina’s TruSeq Nano DNA Sample Preparation protocol ...
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bioRxiv - Immunology 2020Quote: ... cells were harvested in Trizol at the specified time points and RNA was isolated using Direct-zol RNA Miniprep kits (Zymo Research) with 1-hour DNase treatment ...
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bioRxiv - Genetics 2019Quote: 25-30 eyes were dissected from both WT and pitx2ΔCE5-7 eye zebrafish embryos at 3 dpf in Ringer buffer and genomic DNA was purified using Quick-DNA microprep Plus kit (ZYMO Research). Eyes were resuspended in Solid Tissue buffer containing Proteinase K and incubated at 55°C for 6 hours ...
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bioRxiv - Cell Biology 2019Quote: ... DNA fragments used for probe library generation were amplified by PCR and recovered by DNA Cleanup kit (D4014, Zymo research, U.S.A.). The amplified probe library could serve as templates for Tn5-FISH probes ...
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bioRxiv - Synthetic Biology 2020Quote: ... yeast strain was used to make competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research).
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bioRxiv - Bioengineering 2019Quote: Knock-in at the AAVS1 locus was verified through PCR on genomic DNA extracted with a Quick-DNA kit (D3024, Zymo Research). For the positive knock-in screen ...
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bioRxiv - Bioengineering 2019Quote: ... 1.5-mL aliquots of cell culture were used as input in minipreps with the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: The cDNA of all single cells was collected together and further concentrated with the DNA Clean and Concentrator−5 kit (Zymo Research). The Exonuclease I (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... 5 heads of each genotype were pooled and total RNA extracted using the Direct-zol RNA Microprep kit (Zymo Research, R2060) and the quality determined using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2021Quote: ... Isolation of the plasmid DNA from bacterial clones was conducted using Zymo or Qiagen mini prep and maxi prep kits (Qiagen #27104, Zymo #D4208T)
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bioRxiv - Genomics 2021Quote: To determine differences due to RNA extraction we isolated RNA using columns from the Direct-zol RNA MicroPrep Kit (Zymo, R2062) (condition ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from six whole 20-21 dpf offspring (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer to check that the samples had an RNA Integrity Number (RIN ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from adult testis or from enriched fractions of spermatogonia with the Direct-zol RNA MiniPrep Plus kit (Zymo Research). RNA (2.0μg ...