Labshake search
Citations for Zymo Research :
5201 - 5250 of 6511 citations for Rat Golgi phosphoprotein 3 GOLPH3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The un-crosslinked sample was ethanol precipitated and purified using the large fragment DNA recovery kit (Zymo Research, D4046). Religated-biotinylated restriction fragments were pulled down using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... These cells were cultured in LB media and plasmids were purified using ZymoPURE II Plasmid Midiprep kit (Zymo Research). Each plasmid was then digested with EcoRI-HF and BamHI-HF (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... Adaptor-ligated fragments were then bisulfite-treated using the EZ DNA Methylation-Lightning™ Kit (Zymo Research, Cat# D5459). Preparative-scale PCR (16 cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s instructions and RNA was extracted using Direct-zol RNA mini kit (Zymo Research, Irvine CA) according to the manufacturer’s instructions and stored in - 70°C till further use ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA of the snap frozen cells was extracted using the Quick-DNA Midiprep Plus Kit (Zymo Research, D4075). sgRNA amplification was performed following a previously published protocol(49) ...
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bioRxiv - Neuroscience 2023Quote: ... Free RNAs were extracted using phenol/chloroform and purified with the RNA Clean & Concentrator-5 kit (Zymo Research, R1015).
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bioRxiv - Microbiology 2023Quote: ... The RNA samples were cleaned and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... and sequence confirmed (Sequetech) and then maxi-prepped per the manufacturer’s protocols (ZymoPure II Plasmid Maxiprep Kit, Zymo Research). Plasmids were sterile filtered using a 0.22-μm syringe filter and stored at 4 °C.
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bioRxiv - Microbiology 2023Quote: ... and the RNA samples were extracted using TRIzol reagent and purified using Direct-zol RNA Purification Kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was captured on protein A/G agarose beads and purified using the ChIP DNA Clean & Concentrator kit (Zymo) and used for qPCR ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction and DNase treatment were performed using a Direct-zolTM RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA (gDNA) was purified from ES cells and tail clips using the Quick-DNA Miniprep Kit (Zymo Research). Initial validation of target insertion was performed with primers designed to amplify across the 5’ and 3’ integration sites (Supplementary data 1-4) ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted with Direct-zol RNA miniprep kit with on-column DNase I digestion (Zymo Research #R2053). 0.5 to 1 μg of total RNA was used for reverse-transcription with ReverTra Ace qPCR RT master mix (Toyobo #FSQ-101 ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from transduced HEK 293 cells using the Zymo Quick-RNA Microprep Kit (Zymo Research, #R1050). Reverse transcription was performed with Invitrogen’s SuperScript IV VILO Master Mix with ezDNAse enzyme (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA from the DRG and SGC cultures was extracted using the Direct-zol RNA Miniprep Kit (Zymo Research), and 0.5–1 μg RNAs were reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were placed on ice and then purified using Zymo DNA clean and concentrator kit -5 (Zymo #D4004) following cDNA protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and 150 µL of 100% ethanol and then purified through a column from the purification kit (Zymo Research, R1015) followed by quality check with a Bioanalyzer small RNA kit (Agilent).
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was isolated either via chloroform extraction or using a Direct-zol MiniPrep or MicroPrep kit (Zymo Research).
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bioRxiv - Genomics 2023Quote: ... according to manufacturer’s instructions followed by a clean up using the RNA Clean & Concentrator-5 kit (Zymo Research, R1013). Concentrations were determined using the Qubit 2.0 Fluorometer ...
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bioRxiv - Genomics 2023Quote: ... At least four million IL-1βlow and IL-1βhigh cells were collected for genomic DNA extraction using Quick-DNA FFPE Kit (Zymo Research). PCR reactions containing up to 10 μg genomic DNA in each reaction were performed using ExTaq DNA Polymerase with primers to amplify the sgRNAs ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted following the manufacturer’s recommendations of the magnetic bead based Quick-DNA MagBead Plus Kit (Zymo Research). The DNA concentration of each sample was measured using a Qubit fluorometer (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... The total RNA and RF samples were purified using a modified RNA Clean & Concentrator-5 kit method (Zymo Research). The volume of each sample was increased to 100 µl ...
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bioRxiv - Genomics 2023Quote: ... and stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from transduced HOS cells using a Quick-DNA Miniprep kit (Zymo Research, Irvine, CA, USA). Vector titers were determined by calculating the ratio between the copies of HIV psi and every two copies of RPP30 via QX200 digital droplet PCR system (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 ng genomic DNA (gDNA) was used for bisulfite conversion using the EZ DNA methylation kit (Zymo Research, D5001). The bisulfited gDNA was denatured and neutralized for amplification ...
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bioRxiv - Cancer Biology 2023Quote: RNAs from respective cell lines were isolated and purified via the Zymo Quick-RNA microprep kit (Zymo Research, #R1058). 1000 ng of DNAse-treated RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad ...
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bioRxiv - Immunology 2023Quote: ... cells and tissue were harvested in TRIzol and RNA was isolated using Direct-zol RNA Miniprep kits (Zymo Research) with 1 h DNase treatment ...
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bioRxiv - Neuroscience 2023Quote: ... The amplicon DNA size was confirmed by gel electrophoresis and purified using the Zymoclean Gel DNA Recovery Kit (Zymo). DNA sequencing library of the purified CASP4 PCR amplicons was prepared using the Native Barcoding Kit (cat no ...
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bioRxiv - Genetics 2024Quote: ... C shaker for 12h-16h prior to harvesting for plasmid purification using ZymoPURE II Plasmid Midiprep kit (Zymo D4200). Based on the colony forming units from the small volumes in the bacterial plates ...
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bioRxiv - Cancer Biology 2024Quote: ... Omega Bio-tek®) and subjected to DNase treatment and purification by the RNA Clean & Concentrator kit (Zymo Research) to yield high-quality RNA for library preparation ...
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bioRxiv - Microbiology 2024Quote: ... the reaction was stopped using RNA binding buffer from the RNA Clean & Concentrator Kit (Zymo Research, Irvine, CA, USA) plus ethanol following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Denmark) for 24 hours prior to total RNA extraction using Direct-zol™ RNA Miniprep kit (Zymo Research, USA). 100-150 islets in technical duplicates were used per condition ...
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bioRxiv - Cancer Biology 2024Quote: ... range: 10.52 ng - 22.9 ng) of cfDNA was bisulfite converted using the EZ DNA Methylation-Lightning Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... PCR products were then identified by agarose gel method and purified via Zymoclean Gel DNA Recovery Kit (Zymo Research). Sanger sequencing of clones was performed by Quintara Biosciences to identify clones that displayed the proper genetic modification ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products of each group were pooled and purified using DNA Clean & Concentrator-25 kit (Zymo Research, #D4034), followed by next-generation sequencing (NGS ...
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bioRxiv - Microbiology 2024Quote: ... total DNA was extracted from approximately 1.0 × 104 neurons using the Quick-DNA™ Miniprep Plus Kit (Zymo Research). qPCR was carried out using PowerUp™ SYBR™ Green Master Mix (ThermoFish Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were pooled and purified with a DNA Clean & Concentrator purification kit (Zymo Research, San Diego, CA, USA). The pooled amplicon library was sequenced on an Illumina MiSeq platform at the Idaho State University Molecular Research Core Facility (RRID:SCR_012598).
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bioRxiv - Developmental Biology 2024Quote: ... Polysome-bound RNA was retrieved from Dynabeads and DNase-treated using the Direct-zolTM RNA MicroPrep kit (Zymo Research).
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNA (3µg) was purified with RNA Clean and Concentrator-5 kit (Zymo Research, Irvine, CA, USA, Ref. ZR1016), keeping only small RNAs fragments (17 - 200 nt ...
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bioRxiv - Neuroscience 2024Quote: ... knockdown efficiency was calculated via qPCR: RNA was extracted with the Zymo Direct-Zol RNA microprep kit (#R2062, Zymo); cDNA synthesis was performed with the SuperScript IV First Strand Synthesis System using oligo-dT priming (#18091050 ...
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bioRxiv - Microbiology 2024Quote: ... Replicon DNA was isolated after another 6-hour growth using the ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research). Reporter replicon-transfected HEK293T cells were imaged 48 h after transfection on an EVOS M5000 Imaging System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: RNA was extracted from a total of 100 mg of ground frozen shoot tissue with Direct-zol kit (Zymo Research) according to manufacturer’s protocol with in-column DNAse treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... Eluates were then concentrated in a SpeedVac to ∼100 µL and purified with the DNA Clean and Concentrator Kit (Zymo). Libraries were sequenced on a HiSeq 2000 at the BioMicroCenter Core Facility at MIT with 50 nt read length.
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was extracted from no treatment and SHX-treated flash frozen cells using the Quick RNA extraction kit (Zymo) following the standard protocol ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was extracted using Direct-zol RNA MicroPrep kit according to manufacturer’s instructions (cat no. R2062, Zymo Research, Irvine CA). Purified RNA samples underwent fragment analysis at the MIT BioMicro Center ...
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bioRxiv - Cell Biology 2019Quote: RNA was typically isolated from confluent monolayers of cells seeded in 10-cm plates using the direct-zol RNA miniprep kit from Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from cells lysed with Trizol using the Direct-zol RNA Miniprep Plus Kit (Zymo Research, R2072). 1 µg of total RNA was reverse transcribed using Maxima H Minus Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... 500 ng of genomic DNA were bisulfite converted using the EZ-96 DNA methylation Gold Kit (Zymo research, Irvine, USA) according to the kit’s manual ...
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bioRxiv - Genetics 2021Quote: IL2 and CD20 constructs were created through PCR amplification from cDNA and genomic DNA respectively.PCR products were purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research) and cloned into the pEntryD-Topo vector ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA from strains that had at least an IC50 shift of 1.5-fold was extracted using the YeaStar Genomic DNA kit (cat. No D2002, ZYMO Research).