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Citations for 3M :
1 - 50 of 58 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... 200 µL two plug StageTips 55 with SDB-RPS (3M EMPORE™, Cat#2241). SDB-RPS StageTips were conditioned with 60 µL isopropanol ...
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bioRxiv - Microbiology 2020Quote: ... containing four different concentrations of NaCl (e.g. 1M, 2M, 3M &4M). After inoculation ...
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bioRxiv - Biochemistry 2021Quote: ... in AC buffer containing decreasing concentrations of guanidine-HCl (6M and 3M), then for 30 min at 4°C in 0.1M guanidine-HCl and finally o.n ...
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bioRxiv - Biochemistry 2020Quote: ... samples were desalted with C18 StageTips containing 3 disks of Empore C18 material (3M).23 In Freiburg ...
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bioRxiv - Neuroscience 2021Quote: Samples were incubated in primary antibody solution (primary antibody in 5% DMSO/0.3M Glycine/PTxwH) for 8 days ...
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bioRxiv - Biochemistry 2019Quote: ... Fully deuterated samples were made by first denaturing the protein (3M guanidine HCl and high heat for at least 30 minutes) ...
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bioRxiv - Bioengineering 2019Quote: ... containing 1 (Fab’) or 2 (mAb) molar equivalents of tri-mutant sortase A Δ59 (3M srt) (23) ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: HeLa cells were treated with MEM media containing the appropriate fluorogen (HBP-3,5DOM, HBP-3,5DM, HBP-3M, HBT-3,5DOM, HBT-3,5DM, HBT-3M, HBO-3,5DM ...
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bioRxiv - Molecular Biology 2022Quote: ... containing stripes of double- sided sticky tape (50 µm thickness (3M-VHB; Sury AG, S1473-M25)) on two sides for attachment ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: HeLa cells were treated with MEM media containing the appropriate fluorogen (HBP-3,5DOM, HBP-3,5DM, HBP-3M, HBT-3,5DOM ...
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bioRxiv - Molecular Biology 2023Quote: ... Briefly: The cell pellet containing expressed untagged RAD15 was thawed in two volumes of lysis buffer (3M NaCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Columns for protein binding were prepared as follows: C18 Discs (Emmore 3M C18) were punched out using a blunted syringe needle and pushed into 200 μl tips before activating with methanol ...
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bioRxiv - Neuroscience 2024Quote: ... and bound proteins were dissociated in 50 μL of 1x loading dye (0.3M Tris·HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel containing RNA was incubated overnight at 42°C in elution buffer (0.3M NaOAc pH 5.5, 2% SDS). RNA was ethanol precipitated and stored at -20°C until use.
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Novel model of distal myopathy caused by the myosin rod mutation R1500P disrupts acto-myosin bindingbioRxiv - Molecular Biology 2019Quote: Protein lysates were prepared by homogenizing hindlimb muscle tissue in myosin extraction buffer (0.3M NaCl ...
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bioRxiv - Immunology 2021Quote: ... The protein thus obtained was mixed with an equal volume of guanidine solution (3M GuHCl ...
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bioRxiv - Genetics 2024Quote: Protein lysates or histone samples were mixed with 5X Loading Buffer (5% SDS, 0.3M Tris pH 6.8 ...
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bioRxiv - Biochemistry 2022Quote: ... and a custom-made flow cell containing 1−2-μm-wide chromium barriers using two-sided tape (3M 665). Single-molecule fluorescent images were collected with an inverted Nikon Ti-E microscope using prism based TIRF microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide mixture was desalted using stage-tips containing 2X styrene divinylbenzene-reverse phase sulfonated (SDB-RPS) (Empore, 3M). StageTips were pre-equilibrated with addition of 100% ACN ...
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bioRxiv - Cell Biology 2019Quote: ... the cultures were fixed using cold paraformaldehyde (4%) for 1 hr and then permeabilized using a buffer containing digitonin (50 µg/ml)/sucrose (0.3M) (22 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 hr and then permeabilized using a buffer containing digitonin or saponin (50 µg/ml) and sucrose (0.3M) (27) ...
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bioRxiv - Biochemistry 2020Quote: ... and a custom-made flow cell containing 1-2-μm-wide chromium barriers using two-sided tape (3M 665). Single-molecule fluorescent images were collected with a prism TIRF microscopy-based inverted Nikon Ti-E microscope ...
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bioRxiv - Biochemistry 2022Quote: ... for 1 hr and then permeabilized using a buffer containing digitonin or saponin (50 µg/ml) and sucrose (0.3M) (44) ...
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bioRxiv - Cancer Biology 2020Quote: Harvested cell culture pellets were resuspended in protein lysis buffer (25mM HEPES pH 7,5; 0,3M NaCl ...
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bioRxiv - Biophysics 2020Quote: ... Ex vivo liver samples were immersed in tubes containing Fluorinert™ FC-40 Electronic Liquid (3M, St. Paul’s, MN, USA), a fluorocarbon liquid which produces a black background in proton images ...
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bioRxiv - Developmental Biology 2020Quote: ... Supernatants volume corresponding to 20 μg of total tryptic peptides per sample were loaded onto C18 StageTips (Rappsilber & Mann, 2007) containing 3 disks of Empore C18 material (3M). Prior to sample loading ...
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bioRxiv - Biophysics 2022Quote: ... an aliquot from the exchanging experiment was sampled and diluted 1:13 into D2O quenching buffer containing (100 mM phosphate, pH 2.1, 50 mM NaCl, 3M GuHCl) at 1 °C ...
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bioRxiv - Microbiology 2022Quote: ... Digested peptides were collected (2500 g for 60 s) and lyophilized before being desalted with custom-made stage-tip containing Empore SPE Extraction Disk (3M). Peptides were eluted with 2% acetonitrile ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were desalted using stage-tips containing a Poly-styrene-divinylbenzene copolymer modified with sulfonic acid groups (SDB-RPS; 3M) material ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant reaction was kept on 60°C and loaded to 8% polyacrylamide-urea denaturing gel (containing 8.3M of urea) for electrophoresis ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: ... a custom-made head plate containing a 5 mm diameter hole was fixed to the skull using veterinary adhesive (Vetbond, 3M). Once the head plate was fixed and stabilized ...
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bioRxiv - Cell Biology 2021Quote: ... for 30 minutes at room temperature and then incubated with primary antibody overnight at 4°C in antibody solution (2.5% BSA, 0.3M Glycine and 1% Tween in DPBS). Murine and human arterial sections were stained with primary antibodies [Supplementary Table I] ...
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bioRxiv - Cell Biology 2021Quote: ... for 30 minutes at room temperature and then incubated with primary antibody overnight at 4°C in antibody solution (2.5% BSA, 0.3M Glycine and 1% Tween in DPBS). Murine and human arterial sections were stained with primary antibodies [Supplementary Table I] ...
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bioRxiv - Developmental Biology 2022Quote: ... Bound protein complexes were trypsinized on bead and peptides were desalted with C18-STAGE tips (3M) as described previously (Zee et al. ...
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bioRxiv - Bioengineering 2019Quote: ... The oil flow inlet was similarly connected via a steel pin and Tygon tubing to a pressurized vessel containing 2% w/w ionic krytox (Miller Stephenson 157 FSH) in HFE-7500 (3M Novec 7500); the wash inlet was connected via a steel pin and Tygon tubing to a pressurized vessel containing 50% v/v ethanol for device flushing ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were desalted and concentrated on a StageTip (Stop and go extraction Tip) containing C18 reverse-phase material (Empore, 3M, Minneapolis, USA). For LC-MS measurement ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein eluates were dialyzed overnight at 4°C in dialysis buffer (0.1M Tris [pH 8.0], 0.3M NaCl) and concentrated using amicon ultra concentrators ...
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bioRxiv - Biophysics 2023Quote: ... Different concentrations of protein samples were mixed with 4X Loading buffer (0.25 M Tris-HCl pH 6.8, 8% SDS, 0.3M DTT ...
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bioRxiv - Systems Biology 2020Quote: ... Incubation with primary antibodies (0.3M glycine, 0.1% Tween 20, in PBS) was performed overnight in a humidified dark chamber at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 250 ng of trypsin was added for the digestion which was then stopped with 1µL of 100% FA and peptides were purified with StageTips (32) containing C18 reverse phase (3M Empore C18 Extraction Disks). Samples were resuspended in 10μL of 2% ACN ...
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bioRxiv - Biochemistry 2022Quote: For the peptide desalting and cleanup the in-house made stage tips containing SDB-RPS membrane (Empore-3M, CDS Analytical, Oxford, PA, USA) were used ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 1 hour at room temperature in Blocking Solution (PBS containing 1% BSA, 10% FBS, 0.3M glycine and 0.1% Tween-20). The fixed cells were incubated with a mixture of the primary antibodies in PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and resuspended in 50mL MURB protein sample buffer (100mM MES pH7.0, 1% SDS, 3M urea, 10% 2-mercaptoethanol) prior to Western analysis.
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bioRxiv - Cell Biology 2020Quote: ... lysed in protein sample buffer (0.2M Tris HCl, 0.4 M DTT, 277 mM SDS, 6mM Bromophenol Blue, 4.3M glycerol), and boiled and subjected to SDS-PAGE and Western blotting as described above.
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bioRxiv - Neuroscience 2024Quote: ... Resulting protein digests equivalent to 5 µg were desalted using StageTip (SDB-XC, 3M, St. Paul, MN, USA)51 ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with mouse anti-mKO1 monoclonal antibodies (M104-3M, 1/200; MBL) overnight ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with mouse anti-mKO1 monoclonal antibodies (M104-3M, 1/200; MBL), rabbit anti-M-opsin antibodies (AB5405 ...
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bioRxiv - Systems Biology 2020Quote: ... and incubated with secondary antibodies (0.3M glycine, 0.1% Tween 20, in PBS) for 1 hour in a humidified dark chamber at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The pellet containing Golgi membrane was resuspended using 1 ml of washing buffer (25 mM HEPES-KOH pH 7.4, 3M KCl, 2.5 mM Mg(OAc)2 ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets containing the expressed NP constructs were resuspended in lysis buffer (50mM NaH2PO4, pH 7.0, 500mM NaCl, 3M Urea, and 5mM β-mercaptoethanol), supplemented with 1 mM phenyl-methyl sulphonyl fluoride (PMSF ...