Labshake search
Citations for 3M :
1 - 50 of 62 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Novel model of distal myopathy caused by the myosin rod mutation R1500P disrupts acto-myosin bindingbioRxiv - Molecular Biology 2019Quote: Protein lysates were prepared by homogenizing hindlimb muscle tissue in myosin extraction buffer (0.3M NaCl ...
-
bioRxiv - Biochemistry 2023Quote: NuMat was subfractionated by sequential extraction with GdnHCl extraction buffers (1M, 2M, 3M, 4M, 8M) as per the experimental schematic shown in Fig 1 a ...
-
bioRxiv - Microbiology 2021Quote: ... fractionation on an SDB STAGE (Stop and go extraction) tip with 4 punches (Empore reversed phase extraction disks from 3M, SDB-XC reversed phase material ...
-
bioRxiv - Microbiology 2020Quote: ... 3M Empore C18 extraction disks (3M, Minnesota, USA) were packed in 200 μl pipet tips by a blunt-ended needle and a plunger or metal rod that helped fit the extracted disks in the pipet tips ...
-
bioRxiv - Developmental Biology 2019Quote: ... Nuclei pellet were resuspended in an equal volume of Nuclear Lysis Buffer (Hepes 1M, pH7.6, KCl 3M, EDTA 0.5M ...
-
bioRxiv - Neuroscience 2023Quote: ... Desalting of the proteolytic peptide sample was performed on a C18 solid phase extraction column (‘home-made ZipTip’ from a 15G punch from Empore™ octadecyl C18 47 mm extraction disks (3M, Bellefonte)) and after purification proteins were solubilized in 3 % acetonitrile and 0.5 % formic acid (both from Biosolve) ...
-
bioRxiv - Cell Biology 2022Quote: ... reversed phase Empore C18 Extraction Disks (3M, Cat#3M2215), following an established method (Rappsilber et al ...
-
bioRxiv - Genomics 2021Quote: ... a STAGE-TIP protocol using C18 extraction disks (3M Empore) was performed to desalt the samples (62 ...
-
bioRxiv - Genetics 2022Quote: ... desalted on self-made C18 Empore® extraction discs (3M) StageTips(64) ...
-
bioRxiv - Biochemistry 2024Quote: ... Empore C8 extraction disks were purchased from 3M (St. Paul, MN). MS grade Lys-C (lysyl endopeptidase ...
-
bioRxiv - Molecular Biology 2019Quote: ... the reactions were diluted in 200 µL of RNA extraction buffer (0.3M NaOAc ...
-
bioRxiv - Genetics 2022Quote: ... Sample was applied to a double layer Empore C18 Extraction Disk (3M) prepared with methanol ...
-
bioRxiv - Plant Biology 2021Quote: ... Peptides were desalted using C18 Empore® extraction discs (3M, Maplewood, MN) STAGE tips (Rappsilber et al. ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were cleaned up using Empore high performance extraction disk cartridges (3M). Following the clean-up ...
-
bioRxiv - Microbiology 2021Quote: ... Replicate DNA extractions were pooled and concentrated using sodium acetate (0.3M final concentration) and ethanol (2x volume ...
-
bioRxiv - Cancer Biology 2021Quote: ... vacuum-centrifuged to dryness and subjected to C18 solid-phase extraction desalting (3M Empore). High-pH reversed-phase (RP ...
-
bioRxiv - Microbiology 2021Quote: Home-packed strong cation-exchange chromatography StageTip with Empore Cation-SR Extraction material (3M) was sequentially conditioned by 100 μL ACN ...
-
bioRxiv - Immunology 2020Quote: ... having been washed with low cold salt extraction buffer (0.3M sodium phosphate, pH 7.6), was treated with 1:5 trypsin ...
-
bioRxiv - Biochemistry 2023Quote: ... Empore SDB-XC solid-phase extraction disks were purchased from 3M (St. Paul, MN). S-Trap micro columns were purchased from ProtiFi (Huntington ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were fractionated on SDB-XC extraction disks (3M, 1.07 mm x 0.50 mm i.d.) under high pH reversed phase conditions into 7 fractions (5,10 ...
-
bioRxiv - Biochemistry 2021Quote: ... The StageTips were made by placing a small portion of Empore solid phase extraction disks (3M) in a 200 µL pipette tip ...
-
bioRxiv - Biochemistry 2022Quote: ... gel fractions corresponding to 20–30 nt were harvested and crushed in extraction buffer (0.3M NaCl). After overnight rotation at 4°C ...
-
bioRxiv - Biophysics 2019Quote: ... Salt-extraction of axonemes was performed by incubating with the indicated amount of KCl (0.3M - 1M) + HMDE for 20 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... The peptides were eluted from the sorbent (Empore™ SDB-XC solid phase extraction discs, 3M, 2240) with 60% acetonitrile in water (60 μL) ...
-
bioRxiv - Cell Biology 2022Quote: ... the samples were desalted using Stage Tips with C18 membranes (Octadecyl C18-bonded silica - 3M Empore extraction disks) and thoroughly dried in an evaporator (SPD 1010 SpeedVac ...
-
bioRxiv - Molecular Biology 2019Quote: ... the beads were washed 3 times with ice-cold NET-150 buffer and suspended in 200 µL of RNA extraction buffer (0.3M NaOAc ...
-
bioRxiv - Biophysics 2020Quote: ... The resulting peptides were desalted using a Stage Tip manually packed with Empora C18 High Performance Extraction Disks (3M) (Rappsilber et al. ...
-
bioRxiv - Neuroscience 2019Quote: ... proteolytic peptides were desalted by stop and go extraction (STAGE) with self-packed C18 tips (Empore C18 SPE, 3M) (Rappsilber et al. ...
-
bioRxiv - Neuroscience 2021Quote: ... The resulting peptides were desalted using a Stage Tip manually packed with Empora C18 High Performance Extraction Disks (3M) (Rappsilber ...
-
bioRxiv - Microbiology 2022Quote: ... Digested peptides were collected (2500 g for 60 s) and lyophilized before being desalted with custom-made stage-tip containing Empore SPE Extraction Disk (3M). Peptides were eluted with 2% acetonitrile ...
-
bioRxiv - Biochemistry 2019Quote: ... Digested samples were acidified to
extraction tips (StageTips) composed of C18 Empore material (3M) packed in to 200 µl pipette tips (Ishihama et al ... -
bioRxiv - Immunology 2020Quote: ... Digested peptides were acidified with 10% formic acid (FA) to pH ∼2 and desalted using stage tips with Empore C18 SPE Extraction Disks (3M) and dried under vacuum.
-
bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped by acidification with TFA (1% [v/v] final concentration) and the peptide sample was desalted using a EmporeTM Solid Phase Extraction cartridge (3M Bioanalytical Technologies ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Digested peptides were acidified with 10% formic acid to pH ~2 and desalted using stage tips with Empore C18 SPE Extraction Disks (3M) and dried under vacuum.
-
bioRxiv - Microbiology 2023Quote: ... samples were acidified to 0.3% formic acid with 10% formic acid and cleaned up with an MCX STAGE tip (39) using Empore SDB-RPS extraction disks material from 3M. The eluted samples were Speedvaced to dryness.
-
bioRxiv - Molecular Biology 2020Quote: ... and loaded onto StageTips carrying four layers of C18 disc material (punch-outs from 47mm C18 3M™ extraction discs, Empore). Elution was performed with 25% of acetonitrile (ACN ...
-
bioRxiv - Neuroscience 2023Quote: ... Desalting of peptides was done using hand packed SPE Empore C18 Extraction Disks (aka Stage Tips, 3M. St. Paul, MN, USA) as described (95) ...
-
bioRxiv - Cancer Biology 2019Quote: ... pre-extracted in pre-extraction buffer (25mM HEPES pH 7.4, 50mM NaCl, 1mM EDTA, 3mM MgCl2, 0.3M sucrose, 0.5% Triton X-100) 5 minutes on ice ...
-
bioRxiv - Cell Biology 2021Quote: ... The peptides were desalted and concentrated on a StageTip (Stop and go extraction Tip) containing C18 reverse-phase material (Empore, 3M, Minneapolis, USA). For LC-MS measurement ...
-
bioRxiv - Microbiology 2019Quote: ... 250 ng of trypsin was added for the digestion which was then stopped with 1µL of 100% FA and peptides were purified with StageTips (32) containing C18 reverse phase (3M Empore C18 Extraction Disks). Samples were resuspended in 10μL of 2% ACN ...
-
bioRxiv - Microbiology 2020Quote: ... after which supernatants containing eluted peptides loaded onto StageTips (9) generated by packing 200 µL pipette tips with four layers of Empore C8 solid phase extraction disk (3M; Cat. # 2214-C8). StageTips were washed by 25°C centrifugation at 1000g for 3 min with 50 µL of 0.1% (v/v ...
-
bioRxiv - Immunology 2023Quote: The peptides were further isolated from the immunoprecipitated peptide-HLA class I complexes using either Sep-Pak C18 cartridges (Waters Corp, Catalog # WAT020515) or stage tips packed manually using C18 extraction disks (20 stacked disks, 3M™ Empore™). The stage tips or the cartridges were activated with 80% acetonitrile in 0.1% TFA followed by a wash with 0.1% TFA ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was loaded onto equilibrated stage tips prepared in house by stacking 3×14G styrene divinylbenzene (SDB)-XC layers (Empore Solid Phase Extraction Products, 3M, Eagan, MN, USA) into a 200 μL Eppendorf tip (Granata et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... the beads are collected from the final wash and resuspended in 500ul of RNA Extraction Buffer (0.3M NaOAc pH 5.3, 1mM EDTA, 1% SDS), 100ul acid phenol ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA fragments between 50-80nt were cut out and eluted overnight in RNA gel extraction buffer (0.3M NaOAc, pH5.5, 1mM EDTA, 0.25% v/v SDS). RNA fragments were dephosphorylated using T4 PNK (NEB) ...
-
bioRxiv - Biochemistry 2019Quote: ... Fully deuterated samples were made by first denaturing the protein (3M guanidine HCl and high heat for at least 30 minutes) ...
-
bioRxiv - Biochemistry 2022Quote: ... dissolved in 200 mM NH4HCO2 (pH 10, 300 µL), then subsequently fractionated using a homemade stage tip (three layers of SDB-XC extraction disks, (3M, 1.07 mm x 0.50 mm i.d.)) in a 200 µL pipette tip ...
-
bioRxiv - Developmental Biology 2023Quote: ... Columns for protein binding were prepared as follows: C18 Discs (Emmore 3M C18) were punched out using a blunted syringe needle and pushed into 200 μl tips before activating with methanol ...
-
bioRxiv - Neuroscience 2024Quote: ... and bound proteins were dissociated in 50 μL of 1x loading dye (0.3M Tris·HCl ...
-
bioRxiv - Immunology 2021Quote: ... The protein thus obtained was mixed with an equal volume of guanidine solution (3M GuHCl ...