Labshake search
Citations for 3M :
51 - 100 of 105 citations for Contactin Associated Protein Like 3 CNTNAP3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... the beads were washed 3 times with ice-cold NET-150 buffer and suspended in 200 µL of RNA extraction buffer (0.3M NaOAc ...
-
Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... washed in PBS and incubated in a blocking/permeabilizing solution (0.3M Glycine, 0.05%Triton-X, 3% BSA, 1X PBS) for 30 min at RT ...
-
bioRxiv - Systems Biology 2020Quote: ... the nuclei pellet was resuspended in 3 mL buffer consisted of one part buffer B and two parts buffer C (2.3M sucrose ...
-
bioRxiv - Neuroscience 2020Quote: ... and Western Blotting samples were denatured at 94°C for 5 minutes in 3 x SDS sample buffer (150mM Tris pH 8, 6% SDS, 0.3M DTT ...
-
Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Antibodies were then eluted by incubating the samples in an elution buffer (0.5 M L-glycine, 3 M Urea, 3M Guanidium Chloride ...
-
bioRxiv - Biochemistry 2022Quote: ... Pooled TMT labeled peptides were fractionated using the high pH reverse-phase peptide fractionation kit (Pierce) into 3 fractions (20%, 25%, and 50% acetonitrile in 0.1% triethylamine) and desalted with Empore-C18 (3M) in-house packed StageTips prior to analysis by mass spectrometry ...
-
bioRxiv - Neuroscience 2023Quote: ... we glued its backside to the copper plate using 3-component dentist glue (Protemp™ II, 3M, Neuss, Germany). A fine tetrode wire (Redi Ohm 800 ...
-
bioRxiv - Neuroscience 2023Quote: ... A generalized linear model was constructed where 4 weighted trials and a constant would predict the response (i.e., R = β1M-3 + β2M-2 + β3M-1 + β4M0 + β5 ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were rinsed three times with dH20 and incubated in equal amounts of dH20 and elution buffer (3×5min; 0.5M L-glycine, 3M urea, 3M guanidine hydrochloride ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were rinsed three times with dH20 and incubated in equal amounts of dH20 and elution buffer (3×5min; 0.5M L-glycine, 3M urea ...
-
bioRxiv - Neuroscience 2023Quote: ... by cutting a 5 mm square hole in 3 layers of Highland electrical insulation tape (3M, Saint Paul, Minnesota) and covering the bottom with a translucent white plastic square (cut from a Farmland Traditions dog treat bag) ...
-
bioRxiv - Cancer Biology 2020Quote: Harvested cell culture pellets were resuspended in protein lysis buffer (25mM HEPES pH 7,5; 0,3M NaCl ...
-
bioRxiv - Developmental Biology 2020Quote: ... Supernatants volume corresponding to 20 μg of total tryptic peptides per sample were loaded onto C18 StageTips (Rappsilber & Mann, 2007) containing 3 disks of Empore C18 material (3M). Prior to sample loading ...
-
bioRxiv - Bioengineering 2021Quote: ... we performed a direct analysis of the reduced model form to MAD and AERS independently (e.g., MADD2 = β0 + β1rsF CREST 1,i + β2rsF CREST 3 −REST 1,i + β3M ADD1), including the day one term to account for effects of previous behavior.
-
bioRxiv - Pathology 2022Quote: Adult timed pregnant female olive baboons (early gest n=4 Dams 1-4E; mid-gest n=3, Dams 1-3M) were utilized for this study ...
-
bioRxiv - Biophysics 2021Quote: ... using two strips (3 mm wide) of optically clear double-stick adhesive tape (50 µm thick, 3M™, #8212, USA). 50 µl of actin or actin-DNA sensor solution was carefully deposited in the middle of the chamber before closing it ...
-
bioRxiv - Neuroscience 2021Quote: ... A 3 mm coverslip was placed on the brain and fixed with dental cement (RelyX®, 3M-ESPE, Neuss, Germany). More than twenty GFAP-EGFP/PLP-DsRed1 and ten PLP-EGFPmem/GFAP-mRFP1 mice were investigated up to ten times in between 30 days after the injury at different time intervals ...
-
bioRxiv - Genomics 2019Quote: ... We filled a 200 μl pipette tip with 6 mg of C18 matrix (Reprosil-Pur Basic C18, 3 μm, Dr. Maisch GmbH) on top of a C18 disk plug (EmporeTM C18, 3M). We dissolved 50 μg of vacuum-dried peptides with 150 μl of pH10 ABC buffer and loaded it onto the pre-equilibrated C18 tip ...
-
bioRxiv - Plant Biology 2021Quote: ... Peptides were desalted and pre-fractionated (in 3 fractions) by stage-tipping using Empore Styrenedivenylbenzene Reversed Phase Sulfonate material (SDB-RPS; 3M) (Kulak et al. ...
-
bioRxiv - Immunology 2020Quote: ... Labeled samples were acidified using triflouroacetic acid (TFA) and cleaned-up using StageTip (3-disc plug of C18 Empore material, 3M). StageTip’s were conditioned by rinsing them with (2x ...
-
bioRxiv - Neuroscience 2022Quote: ... Lysates for all WTs within a cohort (Cohort 1: 2F + 3M; Cohort 2: 3M; Cohort 3: 4F; Cohort 4: 6M), and all KOs within a cohort were pooled (Cohort 1 ...
-
bioRxiv - Neuroscience 2022Quote: ... The exposed brain was covered with sterile saline and sealed with a 3-mm square borosilicate coverslip glass (CS-3S, Warner Instruments) using cyanoacrylate glue and tissue adhesive (1469SB; 3M). For nNOS neuron specific GCaMP7f expression ...
-
bioRxiv - Neuroscience 2023Quote: ... and a glass cranial window (diameter, 3 mm; thickness, 100-150 µm) was placed and secured over the craniotomy using cyanoacrylate adhesive (3M) and dental cement ...
-
bioRxiv - Neuroscience 2023Quote: Sharp electrode recording: G150F-3 (borosilicate: standard wall with filament, Warner Instruments) capillaries were loaded with internal solution (3M KCl) through microfil/microloader tips ...
-
bioRxiv - Neuroscience 2023Quote: The proteomics signature had 4 validation data sets: R6/2 mice aged 2 months (R6/2 2M) or 3 months (R6/2 3M) from PRIDE experiment PXD013771 ...
-
bioRxiv - Bioengineering 2023Quote: ... resulting in monodisperse droplets with an average diameter of 18 µm (∼3 pL volume).52 The carrier oil phase was HFE 7500 (3M) with 1% (w/w ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were digested with LysC and trypsin overnight at room temperature and purified on StageTips with 3 layers of C18 Empore filter discs (3M) and analyzed by LC-MS/MS using an Easy nanoLC system coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... for 30 minutes at room temperature and then incubated with primary antibody overnight at 4°C in antibody solution (2.5% BSA, 0.3M Glycine and 1% Tween in DPBS). Murine and human arterial sections were stained with primary antibodies [Supplementary Table I] ...
-
bioRxiv - Cell Biology 2021Quote: ... for 30 minutes at room temperature and then incubated with primary antibody overnight at 4°C in antibody solution (2.5% BSA, 0.3M Glycine and 1% Tween in DPBS). Murine and human arterial sections were stained with primary antibodies [Supplementary Table I] ...
-
bioRxiv - Developmental Biology 2022Quote: ... Bound protein complexes were trypsinized on bead and peptides were desalted with C18-STAGE tips (3M) as described previously (Zee et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were acidified using 1% TFA and peptides separated into 3 fractions on a 200 µL StageTips packed with three Empore™ SPE Disks SDB-RPS (3M) according to a previously published protocol (Kulak et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... were pulled to a fine tip (PN-3,Narishige) to create recording electrodes (25–50 M resistance, backfilled with 3M KCl) and firepolished (Microforge MF-830 ...
-
bioRxiv - Bioengineering 2021Quote: ... The membrane strips were then fixed in place by 3 mm wide and 2 cm long double-sided tape strips (3M, USA) placed parallel to the longest side of the HoC ...
-
bioRxiv - Neuroscience 2022Quote: ... and all KOs within a cohort were pooled (Cohort 1: 2F + 3M; Cohort 2: 3M; Cohort 3: 4F; Cohort 4: 6M) to allow for sufficient starting material ...
-
bioRxiv - Neuroscience 2023Quote: ... The pipette was removed after 3-4 minutes waiting time and the wound edges were stitched or closed by Vetbond surgical glue (3M, Hungary). The animals were continuously monitored and observed for the signs of pain ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein eluates were dialyzed overnight at 4°C in dialysis buffer (0.1M Tris [pH 8.0], 0.3M NaCl) and concentrated using amicon ultra concentrators ...
-
bioRxiv - Biophysics 2023Quote: ... Different concentrations of protein samples were mixed with 4X Loading buffer (0.25 M Tris-HCl pH 6.8, 8% SDS, 0.3M DTT ...
-
bioRxiv - Systems Biology 2020Quote: ... Incubation with primary antibodies (0.3M glycine, 0.1% Tween 20, in PBS) was performed overnight in a humidified dark chamber at 4°C ...
-
bioRxiv - Bioengineering 2021Quote: ... resin composite layers were built up in 1-mm-thick increments to 3 mm thickness (Z250, 3M ESPE, St. Paul, MN, USA). After being stored in deionized water at 37°C for 24 h ...
-
bioRxiv - Cell Biology 2021Quote: ... and resuspended in 50mL MURB protein sample buffer (100mM MES pH7.0, 1% SDS, 3M urea, 10% 2-mercaptoethanol) prior to Western analysis.
-
bioRxiv - Cell Biology 2020Quote: ... lysed in protein sample buffer (0.2M Tris HCl, 0.4 M DTT, 277 mM SDS, 6mM Bromophenol Blue, 4.3M glycerol), and boiled and subjected to SDS-PAGE and Western blotting as described above.
-
bioRxiv - Neuroscience 2024Quote: ... Resulting protein digests equivalent to 5 µg were desalted using StageTip (SDB-XC, 3M, St. Paul, MN, USA)51 ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was loaded onto equilibrated stage tips prepared in house by stacking 3×14G styrene divinylbenzene (SDB)-XC layers (Empore Solid Phase Extraction Products, 3M, Eagan, MN, USA) into a 200 μL Eppendorf tip (Granata et al. ...
-
bioRxiv - Bioengineering 2019Quote: ... incubated with mouse anti-mKO1 monoclonal antibodies (M104-3M, 1/200; MBL) overnight ...
-
bioRxiv - Bioengineering 2019Quote: ... incubated with mouse anti-mKO1 monoclonal antibodies (M104-3M, 1/200; MBL), rabbit anti-M-opsin antibodies (AB5405 ...
-
bioRxiv - Systems Biology 2020Quote: ... and incubated with secondary antibodies (0.3M glycine, 0.1% Tween 20, in PBS) for 1 hour in a humidified dark chamber at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined with a BCA protein assay kit (23225, Thermo Fischer Scientific) and cell lysates were incubated with 5X Reducing Sample Buffer (0.3M Tris-HCL pH6.8 ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with ten column volumes of elution buffer (0.1M Tris HCl, pH 7.5, 0.3M Nacl, 250mM imidazole) in fractions of 1.0 ml in DNA/protease-free Eppendorf tubes ...
-
bioRxiv - Microbiology 2021Quote: ... faecalis corresponding to 2-3 × 106 CFU was added to the wound site and sealed with a transparent dressing (Tegaderm™ 3M, St Paul Minnesota, USA). At the indicated time points ...
-
bioRxiv - Molecular Biology 2020Quote: Purified His6-KIAA1841(BTB) protein at 0.1mg/ml in sample buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP) was crosslinked with 5-fold increasing concentration of glutaraldehyde (i.e ...