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Citations for 3M :
201 - 250 of 257 citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Samples were acidified using 1% TFA and desalted using 200 µL StageTips packed with three Empore™ SPE Disks C18 (3M) (Rappsilber et al. ...
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bioRxiv - Neuroscience 2019Quote: ... and a small aluminum bolt of 1 cm in length was secured to the surface using dental cement (3M ESPE KETAC). When the cement is set ...
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bioRxiv - Biochemistry 2021Quote: ... Trifluoroacetic acid (0.1 %) was added to the digested peptides and desalted using StageTips packed with a solid phase C18 column disk (3M Empore) with porous graphitic carbon (PGC ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogel vaccines received two (double) doses of antigen and a dose adjuvant (20 μg of Pam3CSK4, CpG1826, cGAMP, or 1 μg of 3M-052). For soluble groups ...
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bioRxiv - Bioengineering 2023Quote: ... Devices were treated with (tridecafluoro-1,1,2,2-tetrahydrooctyl) trichlorosilane (1% v/v) (Gelest) in fluorinated oil HFE 7500 (3M, Saint Paul, MN, USA) and left for solvent evaporation at 55 °C.
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bioRxiv - Developmental Biology 2022Quote: ... Protein eluates were dialyzed overnight at 4°C in dialysis buffer (0.1M Tris [pH 8.0], 0.3M NaCl) and concentrated using amicon ultra concentrators ...
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bioRxiv - Developmental Biology 2020Quote: ... Supernatants volume corresponding to 20 μg of total tryptic peptides per sample were loaded onto C18 StageTips (Rappsilber & Mann, 2007) containing 3 disks of Empore C18 material (3M). Prior to sample loading ...
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bioRxiv - Biophysics 2021Quote: ... using two strips (3 mm wide) of optically clear double-stick adhesive tape (50 µm thick, 3M™, #8212, USA). 50 µl of actin or actin-DNA sensor solution was carefully deposited in the middle of the chamber before closing it ...
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bioRxiv - Neuroscience 2021Quote: ... A 3 mm coverslip was placed on the brain and fixed with dental cement (RelyX®, 3M-ESPE, Neuss, Germany). More than twenty GFAP-EGFP/PLP-DsRed1 and ten PLP-EGFPmem/GFAP-mRFP1 mice were investigated up to ten times in between 30 days after the injury at different time intervals ...
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bioRxiv - Genomics 2019Quote: ... We filled a 200 μl pipette tip with 6 mg of C18 matrix (Reprosil-Pur Basic C18, 3 μm, Dr. Maisch GmbH) on top of a C18 disk plug (EmporeTM C18, 3M). We dissolved 50 μg of vacuum-dried peptides with 150 μl of pH10 ABC buffer and loaded it onto the pre-equilibrated C18 tip ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were desalted and pre-fractionated (in 3 fractions) by stage-tipping using Empore Styrenedivenylbenzene Reversed Phase Sulfonate material (SDB-RPS; 3M) (Kulak et al. ...
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bioRxiv - Immunology 2020Quote: ... Labeled samples were acidified using triflouroacetic acid (TFA) and cleaned-up using StageTip (3-disc plug of C18 Empore material, 3M). StageTip’s were conditioned by rinsing them with (2x ...
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bioRxiv - Neuroscience 2022Quote: ... The exposed brain was covered with sterile saline and sealed with a 3-mm square borosilicate coverslip glass (CS-3S, Warner Instruments) using cyanoacrylate glue and tissue adhesive (1469SB; 3M). For nNOS neuron specific GCaMP7f expression ...
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bioRxiv - Neuroscience 2023Quote: ... and a glass cranial window (diameter, 3 mm; thickness, 100-150 µm) was placed and secured over the craniotomy using cyanoacrylate adhesive (3M) and dental cement ...
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bioRxiv - Neuroscience 2023Quote: Sharp electrode recording: G150F-3 (borosilicate: standard wall with filament, Warner Instruments) capillaries were loaded with internal solution (3M KCl) through microfil/microloader tips ...
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bioRxiv - Bioengineering 2023Quote: ... resulting in monodisperse droplets with an average diameter of 18 µm (∼3 pL volume).52 The carrier oil phase was HFE 7500 (3M) with 1% (w/w ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested with LysC and trypsin overnight at room temperature and purified on StageTips with 3 layers of C18 Empore filter discs (3M) and analyzed by LC-MS/MS using an Easy nanoLC system coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei were lysed by adding one volume of ice-cold Lysis Buffer B (10 mM HEPES pH 7.6, 1mM DTT, 7.5 mM MgCl2, 0.2 mM EDTA, 0.3M NaCl, 1M urea, 1% NP-40) and kept on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... of cold nuclei lysis buffer (10mM HEPES, pH 7.6, 1mM DTT, 7.5mM MgCl2, 0.2mM EDTA, 0.3M NaCl, 1M Urea, 1% NP-40) was added ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were acidified using 1% TFA and peptides separated into 3 fractions on a 200 µL StageTips packed with three Empore™ SPE Disks SDB-RPS (3M) according to a previously published protocol (Kulak et al. ...
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bioRxiv - Neuroscience 2020Quote: ... were pulled to a fine tip (PN-3,Narishige) to create recording electrodes (25–50 M resistance, backfilled with 3M KCl) and firepolished (Microforge MF-830 ...
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bioRxiv - Bioengineering 2021Quote: ... The membrane strips were then fixed in place by 3 mm wide and 2 cm long double-sided tape strips (3M, USA) placed parallel to the longest side of the HoC ...
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bioRxiv - Genomics 2019Quote: ... Slides were washed three times with PBS and incubated with blocking solution (1% Bovine serum albumin, 10% Donkey serum, 0.3M Glycine, 0.1% PBS Tween) for 1 hour at room temperature followed by primary antibody in blocking solution overnight at 4 degrees (see Table 1 for source ...
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bioRxiv - Developmental Biology 2020Quote: ... peptides were desalted with C18 columns and reconstituted in 1% formic acid in water and further separated into five fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Biochemistry 2020Quote: ... The exchange was quenched for 2 min at 1°C with an equal volume of quench buffer (3M guanidine HCl, 0.1% formic acid). Proteins were cleaved with pepsin and separated by reverse-phase chromatography ...
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bioRxiv - Microbiology 2024Quote: ... Thirty dentin slabs of 4 x 4 x 1 mm were obtained and polished with 500 and 1,200 grit sandpaper and finished using Soflex polishing discs (3M, St. Paul, MN, USA). To obtain an initial value for Knoop surface microhardness (SHi) ...
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bioRxiv - Biochemistry 2021Quote: ... The crude F1 preparation was loaded onto a 5mL HisTrap HP 5 mL column at 4 °C and washed with 300 mL of 97 % wash buffer (10% MeOH, 10% glycerol, 0.3M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The oligos were then ethanol precipitated by addition of 4 μL sodium acetate (3M, pH5.2, AMRESCO, Cat.E521-100 ml) and 110 μL 100% ethanol and cooled to −80c for at least 1 h ...
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bioRxiv - Genomics 2023Quote: ... FAC-sorted cells were pelleted at 600 rcf for 10 minutes in a swinging bucket centrifuge at 4°C and resuspended in cold Buffer I (0.3M Sucrose ...
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bioRxiv - Genomics 2020Quote: ... The reaction mixture was loaded immediately into a 1 ml syringe backfilled with HFE-7500 fluorinated oil (3M, catalog no. 98-0212-2928-5) and injected into a flow-focus droplet maker (Supplementary Fig.7a ...
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bioRxiv - Molecular Biology 2021Quote: ... the beads are collected from the final wash and resuspended in 500ul of RNA Extraction Buffer (0.3M NaOAc pH 5.3, 1mM EDTA, 1% SDS), 100ul acid phenol ...
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bioRxiv - Microbiology 2020Quote: ... Alkaline lysis was performed by adding 200 μl solution II (200 mM NaOH; 1% SDS) followed by 150 μl solution III (3M potassium acetate, pH 5.5). The supernatant was extracted in 400 μl phenol:chloroform:isoamyl alcohol and the upper phase mixed with 600 μl 2-propanol and incubated on ice for precipitation of the DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... An additional 200 μl of cold nuclei lysis buffer (20 mM HEPES pH 7.6, 7.5 mM MgCl2, 0.2 mM EDTA, 0.3M NaCl, 1M urea, 1% NP-40, 1mM DTT) was added to the samples ...
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bioRxiv - Cell Biology 2023Quote: ... The pellet containing Golgi membrane was resuspended using 1 ml of washing buffer (25 mM HEPES-KOH pH 7.4, 3M KCl, 2.5 mM Mg(OAc)2 ...
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bioRxiv - Neuroscience 2019Quote: ... A watertight seal was made between the ear cuticle and the divider hole with dental glue (Protemp 4, 3M ESPE) and nerve 6 was secured into the glue at the ventral border of the tympanum.
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bioRxiv - Neuroscience 2020Quote: ... The wound was irrigated with saline and closed with Vicryl 4-0 absorbable sutures and wound glue (VetaBond 3M, UK). The animals were placed in a thermoregulated recovery box until fully awake.
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bioRxiv - Neuroscience 2021Quote: ... The wound was irrigated with saline and closed with Vicryl 4-0 absorbable sutures and wound glue (VetBond, 3M, UK). Anaesthesia was reversed with i.p ...
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bioRxiv - Neuroscience 2022Quote: ... The wound was irrigated with saline and closed with Vicryl 4-0 absorbable sutures and wound glue (VetBond 3M, UK). Anaesthesia was reversed with s.c ...
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bioRxiv - Neuroscience 2019Quote: Rats respond to intra-oral deliveries of Qui in the concentration range used in our experiments (10−4 to 10−3M) with an initial set of non-specific investigative licks that are followed by large ...
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bioRxiv - Molecular Biology 2021Quote: ... Exchange was carried out at room temperature for 4 timepoints (3s, 30s, 300s, 3000s) and terminated by the addition of 20 µl ice-cold quench buffer (3M guanidine – HCl/ 100mM NaH2PO4 pH 2.5/ 1% formic acid) ...
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bioRxiv - Microbiology 2021Quote: ... fractionation on an SDB STAGE (Stop and go extraction) tip with 4 punches (Empore reversed phase extraction disks from 3M, SDB-XC reversed phase material ...
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bioRxiv - Cancer Biology 2019Quote: ... Incisions were closed with 4-0 vicryl suture (Ethicon, Somerville, NJ) and dressed with Tegaderm™ film (3M, Maplewood, MN). All procedures were performed in strict accordance with the NIH Guide for the Care and Use of Laboratory Animals ...
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bioRxiv - Neuroscience 2022Quote: ... The wound edges were then put back in place and the skin sutured with Vicryl 4-0 thread (Ethicon) or surgical glue (Vetbond 3M). Mice were kept on a heating pad until recovery from anesthesia before being returned to their home cages.
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bioRxiv - Cancer Biology 2023Quote: ... post-fixed in 4% PFA and rinsed with PBS + saline-sodium citrate (SSC) buffer (3M NaCl, 0.3 M Sodium citrate), pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was tested on contrasting floors, construction paper (Tru-Ray Heavyweight, Pacon) vs coarse 60-grit sandpaper (ceramic version, 26060PGP-4, 3M). There was no divider between the smooth and rough textured floors ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was loaded onto equilibrated stage tips prepared in house by stacking 3×14G styrene divinylbenzene (SDB)-XC layers (Empore Solid Phase Extraction Products, 3M, Eagan, MN, USA) into a 200 μL Eppendorf tip (Granata et al. ...
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bioRxiv - Genomics 2020Quote: ... Amplification products were purified using a standard protocol for DNA precipitation with sodium acetate and ethanol (1/10 3M sodium acetate, 2 v/v ethanol) (https://www.thermofisher.com/es/es/home/references/protocols/nucleic-acid-purification-and-analysis/dna-protocol/sodium-acetate-precipitation-of-small-nucleic-acids.html#comergent_product_list_15878) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were fixed with ice cold methanol for 5 min and then in blocking buffer (1% BSA, 10 % Horse serum, 0.3M Glycine, 0.2% Triton X in PBS) for 1 h RT ...
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bioRxiv - Cell Biology 2023Quote: ... exchanged into a 1:1 volume mixture of 1X PBST and smiFISH Wash Buffer [10% 20X SSC (20X SSC: 0.3M sodium citrate, 3M NaCl, in nuclease-free H2O, pH7.0), 10% deionized formamide ...
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bioRxiv - Systems Biology 2021Quote: ... we used 20 syringes (Becton Dickinson) filled with 5 ml drug-barcode solutions and 4 syringes filled with 5 ml HFE oil (Novec™ 7500, 3M). These were connected to the inlet ports of the valve chip with PTFE tubing ...