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Citations for 3M :
251 - 300 of 300 citations for 6' ETHYL SPIRO 1 3 DIOXANE 2 3' INDOLIN 2' ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... and then incubated again in blocking solution (0.1% Triton-× 100, 1:50 goat serum, 0.3M glycine) for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Then the cells were incubated with blocking buffer (1XPBS, 1% BSA, 0.3M glycine, 0.1% Tween 20) supplemented with 5% goat serum for 1 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... we used a fluorinated oil and surfactant mixture 1% Picosurf (Sphere Fluidics) in Novec 7500 (3M). One day prior to performing the droplet assay ...
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bioRxiv - Bioengineering 2024Quote: ... at 4°C with 1:400 goat anti-mouse secondary antibody in blocking buffer (10% goat serum, 1% bovine serum albumin, 0.1% sodium citrate, 0.25% Tween-20, 0.25% Triton-X, and 0.3M glycine in 1x phosphate-buffered saline ...
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bioRxiv - Biophysics 2020Quote: ... Low ATP concentrations were verified by absorbance at 259 nm (extinction coefficient: 15.4 × 10−3M−1cm−1). 0.4 ng of 500 nm diameter polystyrene beads (Polysciences ...
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bioRxiv - Molecular Biology 2019Quote: ... Fragmented RNA samples were then mixed with 1/10 volume of Na-acetate (3M, pH 5, Sigma) and 2.5 volumes of EtOH and incubated for 1 hour at −80°C ...
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bioRxiv - Neuroscience 2022Quote: ... A sample (n=1) was formed with 15 heads or bodies in 400μl of HCl (10−3M). The same protocol was used for 1month-old larvae ...
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bioRxiv - Cell Biology 2020Quote: ... cells were lysed with 1 x SDS sample buffer (0.32 M Tris pH 6.8, 10% SDS, 50% glycerol, 3M β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... solubilised with 500µL of 1xNLB (1X PBS, 0.3M NaCl, 1% Triton™ X-100, 0.1% TWEEN® 20) + 1x PhosSTOP ...
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bioRxiv - Molecular Biology 2022Quote: 1 ml of frozen blood sample is thawed and lysed in 10 ml of the NUN buffer (0.3M NaCl ...
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bioRxiv - Physiology 2019Quote: ... Sections were incubated in blocking solution (1%BSA: Sigma Aldrich, 10% normal horse serum: ThermoFisher Scientific, UK 0.3M glycine ...
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bioRxiv - Developmental Biology 2022Quote: ... about 50 μl of pelleted cross-linked cells were resuspended in 350 μl sarkosyl lysis buffer (0.25% sarkosyl, 1 mM DTT and Protease Inhibitor Cocktails in 0.3M RIPA buffer and sonicated at 60% amplification by a tip sonicator (125W ...
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bioRxiv - Plant Biology 2021Quote: Mitochondrial respiration rate measurements were performed using the Clark-type oxygen electrode (Hansatech) with 120 - 240 μg of enriched mitochondria in 1 mL of mitochondrial respiration buffer (0.3M Sucrose ...
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bioRxiv - Neuroscience 2022Quote: ... were recorded (sampling rate 10 kHz) in CA1 using glass borosilicate patch pipettes (∼1-3MΩ, Harvard Apparatus, Kent, UK) and a Multiclamp 700B (Molecular Devices ...
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bioRxiv - Cell Biology 2019Quote: ... the cultures were fixed using cold paraformaldehyde (4%) for 1 hr and then permeabilized using a buffer containing digitonin (50 µg/ml)/sucrose (0.3M) (22 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 hr and then permeabilized using a buffer containing digitonin or saponin (50 µg/ml) and sucrose (0.3M) (27) ...
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bioRxiv - Microbiology 2022Quote: ... 20 μl input of combined cultures were emulsified into ~20,000 1 nl droplets in fluorocarbon oil (3M Novec 7500) stabilized with 2% (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... for 1 hr and then permeabilized using a buffer containing digitonin or saponin (50 µg/ml) and sucrose (0.3M) (44) ...
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bioRxiv - Physiology 2022Quote: ... The pipette was removed 1 min after each injection and the incision was secured using Vetbond tissue adhesive (3M). Subcutaneous injection of sustained release meloxicam (4 mg/kg ...
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bioRxiv - Immunology 2023Quote: ... Cells were then blocked for an hour (PBS with 5% Normal Goat Serum, 1% BSA, 0.05% Triton-X, 0.3M Glycine). Cells were then incubated with respective antibodies (Supplementary table 3 ...
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bioRxiv - Microbiology 2020Quote: ... The protocol used for DNA precipitation included the following steps: for each sample a 1/10 volume of sodium - acetate (3M) was added (pH=5.2) ...
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bioRxiv - Microbiology 2021Quote: ... Digested samples were acidified to a final concentration of 0.5% formic acid and desalted with home-made high-capacity StageTips composed of 1 mg Empore™ C18 material (3M) and 5 mg of OLIGO R3 reverse phase resin (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... an aliquot from the exchanging experiment was sampled and diluted 1:13 into D2O quenching buffer containing (100 mM phosphate, pH 2.1, 50 mM NaCl, 3M GuHCl) at 1 °C ...
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bioRxiv - Microbiology 2021Quote: ... Digested samples were acidified to a final concentration of 0.5% formic acid and desalted with home-made high-capacity StageTips composed of 1 mg Empore™ C18 material (3M) and 5 mg of OLIGO R3 reverse phase resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped by acidification with TFA (1% [v/v] final concentration) and the peptide sample was desalted using a EmporeTM Solid Phase Extraction cartridge (3M Bioanalytical Technologies ...
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bioRxiv - Microbiology 2023Quote: ... 50μg of each plasmid per transfection was ethanol precipitated overnight (2.5 volumes 100% ethanol, 1/10 volumes sodium acetate (3M, pH5.2), - 20°C) ...
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bioRxiv - Neuroscience 2023Quote: ... The larva was moved to a Sylgard platform (∼1 mm thick) in the same Petri dish and glued laterally in place with Vetbond tissue adhesive (3M). A glass capillary was used to apply a drop of Vetbond glue to Sylgard ...
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bioRxiv - Neuroscience 2023Quote: ... Lijers were culled on postnatal day 1 (P1) to even sex ra9os (minimum lijer size: 3M/3F, maximum: 6M/6F) (Fig 1A) ...
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bioRxiv - Immunology 2021Quote: ... and lysed in the presence of lysis buffer (20mM Tris-HCl pH-7.5, 0.3M NaCl, 4mM EDTA, 1% Triton X-100). 1.5mg of total protein was incubated with anti-V5 tag mAb magnetic beads (MBL ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were acidified using 1% TFA and desalted using 200 µL StageTips packed with three Empore™ SPE Disks C18 (3M) (Rappsilber et al. ...
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bioRxiv - Neuroscience 2019Quote: ... and a small aluminum bolt of 1 cm in length was secured to the surface using dental cement (3M ESPE KETAC). When the cement is set ...
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bioRxiv - Biochemistry 2021Quote: ... Trifluoroacetic acid (0.1 %) was added to the digested peptides and desalted using StageTips packed with a solid phase C18 column disk (3M Empore) with porous graphitic carbon (PGC ...
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bioRxiv - Biochemistry 2023Quote: ... Hydrophobic modification of the channels surface was achieved by injecting a solution of 1% (v/v) trichloro (1H,1H,2H,2H-perfluorooctyl) silane in HFE-7500 oil (3M Novec) into the channels ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogel vaccines received two (double) doses of antigen and a dose adjuvant (20 μg of Pam3CSK4, CpG1826, cGAMP, or 1 μg of 3M-052). For soluble groups ...
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bioRxiv - Biochemistry 2023Quote: ... the freshly baked microfluidic chips were flushed with 1% (v/v) trichloro (1H, 1H, 2H, 2H)perfluoroacetylsilane in HFE-7500 (3M-Novec). The chips were heated for 10 minutes at 75°C before storage at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Devices were treated with (tridecafluoro-1,1,2,2-tetrahydrooctyl) trichlorosilane (1% v/v) (Gelest) in fluorinated oil HFE 7500 (3M, Saint Paul, MN, USA) and left for solvent evaporation at 55 °C.
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei were lysed by adding one volume of ice-cold Lysis Buffer B (10 mM HEPES pH 7.6, 1mM DTT, 7.5 mM MgCl2, 0.2 mM EDTA, 0.3M NaCl, 1M urea, 1% NP-40) and kept on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... of cold nuclei lysis buffer (10mM HEPES, pH 7.6, 1mM DTT, 7.5mM MgCl2, 0.2mM EDTA, 0.3M NaCl, 1M Urea, 1% NP-40) was added ...
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bioRxiv - Genomics 2019Quote: ... Slides were washed three times with PBS and incubated with blocking solution (1% Bovine serum albumin, 10% Donkey serum, 0.3M Glycine, 0.1% PBS Tween) for 1 hour at room temperature followed by primary antibody in blocking solution overnight at 4 degrees (see Table 1 for source ...
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bioRxiv - Developmental Biology 2020Quote: ... peptides were desalted with C18 columns and reconstituted in 1% formic acid in water and further separated into five fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Microbiology 2024Quote: ... Thirty dentin slabs of 4 x 4 x 1 mm were obtained and polished with 500 and 1,200 grit sandpaper and finished using Soflex polishing discs (3M, St. Paul, MN, USA). To obtain an initial value for Knoop surface microhardness (SHi) ...
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bioRxiv - Genomics 2020Quote: ... The reaction mixture was loaded immediately into a 1 ml syringe backfilled with HFE-7500 fluorinated oil (3M, catalog no. 98-0212-2928-5) and injected into a flow-focus droplet maker (Supplementary Fig.7a ...
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bioRxiv - Molecular Biology 2021Quote: ... the beads are collected from the final wash and resuspended in 500ul of RNA Extraction Buffer (0.3M NaOAc pH 5.3, 1mM EDTA, 1% SDS), 100ul acid phenol ...
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bioRxiv - Cell Biology 2021Quote: ... for 30 minutes at room temperature and then incubated with primary antibody overnight at 4°C in antibody solution (2.5% BSA, 0.3M Glycine and 1% Tween in DPBS). Murine and human arterial sections were stained with primary antibodies [Supplementary Table I] ...
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bioRxiv - Cell Biology 2021Quote: ... for 30 minutes at room temperature and then incubated with primary antibody overnight at 4°C in antibody solution (2.5% BSA, 0.3M Glycine and 1% Tween in DPBS). Murine and human arterial sections were stained with primary antibodies [Supplementary Table I] ...
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bioRxiv - Microbiology 2020Quote: ... Alkaline lysis was performed by adding 200 μl solution II (200 mM NaOH; 1% SDS) followed by 150 μl solution III (3M potassium acetate, pH 5.5). The supernatant was extracted in 400 μl phenol:chloroform:isoamyl alcohol and the upper phase mixed with 600 μl 2-propanol and incubated on ice for precipitation of the DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... An additional 200 μl of cold nuclei lysis buffer (20 mM HEPES pH 7.6, 7.5 mM MgCl2, 0.2 mM EDTA, 0.3M NaCl, 1M urea, 1% NP-40, 1mM DTT) was added to the samples ...
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bioRxiv - Cell Biology 2023Quote: ... The pellet containing Golgi membrane was resuspended using 1 ml of washing buffer (25 mM HEPES-KOH pH 7.4, 3M KCl, 2.5 mM Mg(OAc)2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were fixed with ice cold methanol for 5 min and then in blocking buffer (1% BSA, 10 % Horse serum, 0.3M Glycine, 0.2% Triton X in PBS) for 1 h RT ...
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bioRxiv - Cell Biology 2023Quote: ... exchanged into a 1:1 volume mixture of 1X PBST and smiFISH Wash Buffer [10% 20X SSC (20X SSC: 0.3M sodium citrate, 3M NaCl, in nuclease-free H2O, pH7.0), 10% deionized formamide ...