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Citations for 3M :
101 - 125 of 125 citations for U3 Small Nucleolar RNA Associated Protein 4 Homolog CIRH1A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined with a BCA protein assay kit (23225, Thermo Fischer Scientific) and cell lysates were incubated with 5X Reducing Sample Buffer (0.3M Tris-HCL pH6.8 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with ten column volumes of elution buffer (0.1M Tris HCl, pH 7.5, 0.3M Nacl, 250mM imidazole) in fractions of 1.0 ml in DNA/protease-free Eppendorf tubes ...
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bioRxiv - Plant Biology 2019Quote: ... stratified at 4°C for 2 days and sown in 12-well microtiter plates sealed with Micropore tape (3M, St. Paul, MN), each well containing 15 ± 2 seeds and 1 mL of filter-sterilized 1X Murashige and Skoog (MS ...
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bioRxiv - Molecular Biology 2020Quote: Purified His6-KIAA1841(BTB) protein at 0.1mg/ml in sample buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP) was crosslinked with 5-fold increasing concentration of glutaraldehyde (i.e ...
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bioRxiv - Molecular Biology 2019Quote: ... Fragmented RNA samples were then mixed with 1/10 volume of Na-acetate (3M, pH 5, Sigma) and 2.5 volumes of EtOH and incubated for 1 hour at −80°C ...
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bioRxiv - Genomics 2023Quote: ... This resulted in 940 samples with RNA derived from head tissue (average gene counts per sample: 3M), and 939 with RNA derived from body tissue (average gene counts ...
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bioRxiv - Genomics 2020Quote: ... the brains were rinsed briefly 4 times with PBS and then washed three times with TNT (0.1M Tris-HCL, 0.3M NaCl, 0.5% Triton X-100), for 15 minutes for the first wash and 5 minutes for the two subsequent washes ...
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bioRxiv - Biochemistry 2021Quote: ... The pH of eluted antibodies was immediately neutralized using sodium acetate (3M, pH 9.0). After protein measurements at 280 nm ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated at room temperature overnight in a solution of antibody diluent (0.3M NaCl ...
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bioRxiv - Immunology 2021Quote: ... of MVA-HIV 1086.c Env construct (in a volume of 0.25 ml divided over left and right biceps) (35) and 15 μg 1086Δ7 gp120 K160N protein mixed with 3M-052 adjuvant in stable emulsion (3M-052-SE) (34 ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel containing RNA was incubated overnight at 42°C in elution buffer (0.3M NaOAc pH 5.5, 2% SDS). RNA was ethanol precipitated and stored at -20°C until use.
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bioRxiv - Molecular Biology 2022Quote: ... RNA was precipitated with 2X volume Molecular Grade Absolute ethanol and 0.1X volume Sodium Acetate (3M, pH 5.2). The samples were then incubated for 1 hour at -20°C and centrifuged at 14,000 g for 15 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the column is washed three times total: once using 400μL RNA Prep Buffer (3M GuHCl in 80% EtOH), twice with 400μL 80% ethanol ...
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bioRxiv - Bioengineering 2022Quote: ... was placed into the pocket and the incision site was sealed using a 4-0 non-absorbable polyamide surgical suture (Redilon ® Pro, Myco Medical) and a few drops of surgical glue (3M Vetbond™ Tissue adhesive). Once ambulatory ...
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bioRxiv - Molecular Biology 2019Quote: ... the beads were washed 3 times with ice-cold NET-150 buffer and suspended in 200 µL of RNA extraction buffer (0.3M NaOAc ...
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bioRxiv - Cell Biology 2023Quote: ... the column is washed three times total: once using 400 μL RNA Prep Buffer (3M GuHCl in 80% EtOH), twice with 400 μL 80% ethanol ...
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bioRxiv - Immunology 2022Quote: ... At 24 hpi cells were harvested to collect intracellular RNA by lysing the cells in 100 μl GITC reagent (3M GITC ...
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bioRxiv - Neuroscience 2022Quote: ... Both primary and secondary antibodies were diluted in TNT (0.1M Tris-HCL, 0.3M NaCl, 0.5% Triton X-100). All confocal microscopy was performed on a Zeiss LSM 700 system ...
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bioRxiv - Molecular Biology 2021Quote: ... the beads are collected from the final wash and resuspended in 500ul of RNA Extraction Buffer (0.3M NaOAc pH 5.3, 1mM EDTA, 1% SDS), 100ul acid phenol ...
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bioRxiv - Molecular Biology 2023Quote: ... and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution: 250ng/μl RNA in 0.3M KCl and 0.015% phenol red). In order to determine functionally relevant amino acids and domains of gcna/acrc ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was UV-crosslinked to membranes using a crosslinker and rRNA was visualized by staining with methylene blue (0.3M NaOAc, 0.02% methylene blue, pH 5.5) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 1000 ng and 500 ng of each RNA sample were spotted to a nylon membrane (Hybond N, Dutscher RPN203B) previously soaked in 2X SSPE solution (0.3M NaCl, 20mM sodium phosphate, 2 mM EDTA) and inserted in the dot blot apparatus (SCIE-PLAS) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA dots were added to a nitrocellulose membrane previously washed with SSC 2X buffer (SSC 20X: NaCl 3M, sodium citrate 300 mM, pH 7). The membrane was incubated for 5 min at 65°C and exposed to 400 mJ/cm2 of 254 nm UV in a UV crosslinker (Biolink Shortwave 254 nm) ...
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bioRxiv - Genomics 2021Quote: ... RNAs were isolated by acid phenol: chloroform and precipitated in 1 ml Ethanol supplemented with (supplemented with 50 µL 3M NaOAc pH 5.2, 1 µl of glycoblue). Pellets were resuspended in 20 ul DEPC-treated H2O ...
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bioRxiv - Immunology 2021Quote: ... Additional high stringency washes were performed after the secondary antibody and Opal fluor applications using high-salt TBST (0.05M Tris, 0.3M NaCl, and 0.1% Tween-20, pH 7.2-7.6). TCT was used as the blocking buffer (0.05M Tris ...