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Citations for 3M :
101 - 127 of 127 citations for Sodium hexanoate 6 6 6 d3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were re-precipitated using a standard ethanol precipitation protocol with sodium acetate (3M, pH 5.2) as the carrier molecule ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1 mg/ml of RNase A in 2x SSC (0.3M NaCl, 30 nM sodium citrate, pH 7.0) was added onto the coverslips and incubated at 37°C for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Isopropanol (4°C, 1:1 volume ratio) and sodium acetate (4°C, 3M, 1:10 volume ratio) were added to the mixture prior to precipitation (−20°C ...
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bioRxiv - Molecular Biology 2024Quote: Precipitate RNA with 2X volume Molecular Grade Absolute ethanol and 0.1X volume Sodium Acetate (3M, pH 5.2). Incubate the samples for 1 hour at -20ºC and centrifuge at 14,000 g for 15 minutes at 4ºC.
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bioRxiv - Neuroscience 2022Quote: ... neural and behavioral data were collected—20min of “passive” recording followed by 120 taste delivery trials in which one of four gustatory stimuli (0.1M sodium chloride [NaCl], 0.3M Sucrose ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was precipitated with 2X volume Molecular Grade Absolute ethanol and 0.1X volume Sodium Acetate (3M, pH 5.2). The samples were then incubated for 1 hour at -20°C and centrifuged at 14,000 g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 150 μL of freshly made bleaching solution (50 µL deionized formamide, 50 µL of 3M sodium chloride/0.3M sodium citrate ...
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bioRxiv - Developmental Biology 2020Quote: ... the probes were removed by four washes in increasing dilutions of 2 X SSC (NaCl 0.3M; Sodium citrate 0.03M) at 70°C (25 ...
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bioRxiv - Cancer Biology 2024Quote: ... EasySep Streptavidin magnetic beads (StemCell) were washed in 100µL 6X SSC (For 20X SSC: 3M NaCl, 0.3M sodium citrate) 3 times before incubation overnight on a rotisserie at 4°C in blocking buffer (2% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... EasySep Streptavidin magnetic beads (StemCell) were washed in 100µL 6X SSC (For 20X SSC: 3M NaCl, 0.3M sodium citrate) 3 times before incubation overnight on a rotisserie at 4°C in blocking buffer (2% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... The oligos were then ethanol precipitated by addition of 4 μL sodium acetate (3M, pH5.2, AMRESCO, Cat.E521-100 ml) and 110 μL 100% ethanol and cooled to −80c for at least 1 h ...
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bioRxiv - Genomics 2024Quote: ... DNA extractions were performed by combining 150 µl of demineralized material with 1.5 ml binding buffer (500 ml Qiagen PB, supplemented with 15 ml Sodium acetate 3M, and 1.25 ml 5M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The protocol used for DNA precipitation included the following steps: for each sample a 1/10 volume of sodium - acetate (3M) was added (pH=5.2) ...
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bioRxiv - Microbiology 2023Quote: ... 50μg of each plasmid per transfection was ethanol precipitated overnight (2.5 volumes 100% ethanol, 1/10 volumes sodium acetate (3M, pH5.2), - 20°C) ...
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bioRxiv - Genetics 2021Quote: ... then rinsed and incubated in two changes of wash buffer C (10% formamide, 2XSSC [0.3M NaCL, 30 mM sodium citrate (7.0)] ...
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bioRxiv - Synthetic Biology 2024Quote: ... all ten reactions of double-digested pR-fp fragments were pooled into a 2mL-DNA LoBind Eppendorf tube and supplemented with 1/10 v/v of sodium acetate (3M, pH5.2) (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Gaussia luciferase activity was assessed 48 hrs after infection by combining cell culture media with Gaussia luciferase substrate (0.1M Tris pH 7.4, 0.3M sodium ascorbate, 10 μM coelenterazine). Luminescence was immediately measured by a BioTek Cytation5 plate reader.
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed with a series of HYB- (50% formamide, 5X SSC with 0.1% Tween) mixed into 2X SSC (0.3M NaCl, 30mM sodium citrate) (75% ...
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bioRxiv - Genomics 2024Quote: ... Beads were washed 4 times with 2x SSC buffer containing 0.1% NP40 (0.3M of NaCl, 0.3M of Sodium citrate, 0.1% NP-40) at 37°C ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... The RNA was eluted in 400 μL RNA elution buffer (EB) at 4°C overnigt (RNA EB: 0.3M sodium acetate (NaOAc, pH 5.5), 1 mM EDTA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The isolated supernatant was mixed with 1500 μL pre-chilled absolute ethanol (Concord Technology, Tianjin) and 50 μL sodium acetate solution (3M, Beijing Chemical Works), kept at −20 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The gels were blotted to Amersham Hybond-N membranes (GE) via overnight capillary transfer with 20x SSC (3M NaCl, 0.3 M sodium citrate). The membranes were crosslinked under UV at 1200×100 μJ/cm2 and then incubated in pre-hybridization buffer (1.5x SSC ...
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bioRxiv - Pathology 2020Quote: ... and 30 ml of lysis buffer were added (Guanidium thiocyanate 0.8M; ammonium thiocyanate 0,4M; sodium acetate 3M, glycerol 5% and Triton-X 100 2%). The material was smashed with a rolling pin ...
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bioRxiv - Cell Biology 2023Quote: ... exchanged into a 1:1 volume mixture of 1X PBST and smiFISH Wash Buffer [10% 20X SSC (20X SSC: 0.3M sodium citrate, 3M NaCl, in nuclease-free H2O, pH7.0), 10% deionized formamide ...
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bioRxiv - Neuroscience 2024Quote: ... BrdU detection required formic acid pre-treatment (formamide 50% in 2× SSC buffer; 2× SSC is 0.3M NaCl and 0.03 M sodium citrate, pH 7.0) at 65 °C for 2 h followed by DNA denaturation for 30 min in 2 M HCl for 30 min at 37°C and rinsed in 0.1 M borate buffer pH 8.5 for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 1000 ng and 500 ng of each RNA sample were spotted to a nylon membrane (Hybond N, Dutscher RPN203B) previously soaked in 2X SSPE solution (0.3M NaCl, 20mM sodium phosphate, 2 mM EDTA) and inserted in the dot blot apparatus (SCIE-PLAS) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA dots were added to a nitrocellulose membrane previously washed with SSC 2X buffer (SSC 20X: NaCl 3M, sodium citrate 300 mM, pH 7). The membrane was incubated for 5 min at 65°C and exposed to 400 mJ/cm2 of 254 nm UV in a UV crosslinker (Biolink Shortwave 254 nm) ...