Labshake search
Citations for 3M :
51 - 83 of 83 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... chromatin was eluted by resuspending beads in 100 ul of Elution buffer (10 mM Tris pH 8.0, 5mM EDTA, 0.1% SDS, 0.3M NaCl) supplemented with 50ugr of proteinase K and incubating at 65C overnight in an agitating thermoblock with heated lid ...
-
bioRxiv - Genetics 2023Quote: ... Samples were then treated with 75µL 10% SDS and 17 µL 5M potassium acetate (3M K, 5M Ac), centrifuged at 15,000 rpm for 5 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... were recorded (sampling rate 10 kHz) in CA1 using glass borosilicate patch pipettes (∼1-3MΩ, Harvard Apparatus, Kent, UK) and a Multiclamp 700B (Molecular Devices ...
-
bioRxiv - Cell Biology 2023Quote: ... exchanged into a 1:1 volume mixture of 1X PBST and smiFISH Wash Buffer [10% 20X SSC (20X SSC: 0.3M sodium citrate ...
-
bioRxiv - Biochemistry 2023Quote: ... and subsequently eluted with 5–10 bead volumes of elution buffer (0.1M Tris [pH 8.0], 0.3M NaCl, 250mM Imidazole). Ni-NTA eluates was concentrated using amicon ultra concentrators ...
-
bioRxiv - Genomics 2023Quote: ... FAC-sorted cells were pelleted at 600 rcf for 10 minutes in a swinging bucket centrifuge at 4°C and resuspended in cold Buffer I (0.3M Sucrose ...
-
bioRxiv - Microbiology 2020Quote: ... The protocol used for DNA precipitation included the following steps: for each sample a 1/10 volume of sodium - acetate (3M) was added (pH=5.2) ...
-
bioRxiv - Molecular Biology 2020Quote: Purified His6-KIAA1841(BTB) protein at 0.1mg/ml in sample buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP) was crosslinked with 5-fold increasing concentration of glutaraldehyde (i.e ...
-
bioRxiv - Immunology 2020Quote: ... Digested peptides were acidified with 10% formic acid (FA) to pH ∼2 and desalted using stage tips with Empore C18 SPE Extraction Disks (3M) and dried under vacuum.
-
bioRxiv - Microbiology 2022Quote: ... Each of these sets was in turn subsampled in a jackknife process to obtain 10 subsets at different depths of sequencing (100K, 500K, 1M, 2M, 3M, 4M ...
-
bioRxiv - Microbiology 2023Quote: ... 50μg of each plasmid per transfection was ethanol precipitated overnight (2.5 volumes 100% ethanol, 1/10 volumes sodium acetate (3M, pH5.2), - 20°C) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Digested peptides were acidified with 10% formic acid to pH ~2 and desalted using stage tips with Empore C18 SPE Extraction Disks (3M) and dried under vacuum.
-
bioRxiv - Molecular Biology 2023Quote: ... mice (25-30g, n= 6-10 mice per group) were injected intraperitoneally with FA (F7876, Sigma-Aldrich, dissolved in 0.3M sodium bicarbonate at a dose of 250 mg/kg) ...
-
bioRxiv - Bioengineering 2024Quote: ... The three syringes and two syringes filled with 10 mL of 5% (w/w) PEG-PFPE surfactant (Ran Biotechnologies) in HFE 7500(3M) HFE 7500 were connected to the microfluidic devices ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2.5 mg of TiO2 beads (GL sciences, 10 µm) were packed in Stage-tips fitted with a 16G-needle punch of C8 material (3M Empore) at the narrow end ...
-
bioRxiv - Microbiology 2020Quote: Cells were washed with PBS and lysed in a NP-40 buffer (10 mM Hepes KOH pH 7.6, 15 mM KCl, 2mM EDTA, 0.15 mM spermine, 0.5 mM spermidine, 10% glycerol, 0.3M sucrose and 0.5% NP-40) supplemented with protease and phosphatase inhibitors plus 0.5mM DTT and incubated for 10 min on ice ...
-
bioRxiv - Genetics 2021Quote: ... then rinsed and incubated in two changes of wash buffer C (10% formamide, 2XSSC [0.3M NaCL, 30 mM sodium citrate (7.0)] ...
-
bioRxiv - Pathology 2020Quote: ... Psoriasis mouse model was established by topically treating each ear with 10 mg of 5% imiquimod cream (Imiquimod Cream 5%; 3M Pharmaceuticals) daily for 5 days ...
-
bioRxiv - Microbiology 2022Quote: ... This was followed by inoculation with 10 μL of respective bacterial cultures per wound before the wound site was sealed with a transparent dressing (Tegaderm 3M, USA). At indicated time points ...
-
bioRxiv - Neuroscience 2023Quote: ... Mice at 8 to 10 weeks of age received a daily topical dose of 5% Imiquimod (IMQ) cream (Aldara; 3M Pharmaceuticals) for 3 or 6 consecutive days ...
-
bioRxiv - Neuroscience 2022Quote: ... Both primary and secondary antibodies were diluted in TNT (0.1M Tris-HCL, 0.3M NaCl, 0.5% Triton X-100). All confocal microscopy was performed on a Zeiss LSM 700 system ...
-
bioRxiv - Molecular Biology 2021Quote: ... Nuclei were lysed by adding one volume of ice-cold Lysis Buffer B (10 mM HEPES pH 7.6, 1mM DTT, 7.5 mM MgCl2, 0.2 mM EDTA, 0.3M NaCl, 1M urea, 1% NP-40) and kept on ice for 10 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... Gaussia luciferase activity was assessed 48 hrs after infection by combining cell culture media with Gaussia luciferase substrate (0.1M Tris pH 7.4, 0.3M sodium ascorbate, 10 μM coelenterazine). Luminescence was immediately measured by a BioTek Cytation5 plate reader.
-
bioRxiv - Neuroscience 2021Quote: ... Acoustic stimuli were presented via an amplification system (A-10, Pioneer Corporation, Tokyo, Japan) and inserted earphones (E-A-RTONE Gold 3A, 3M, St. Paul, Minneapolis).
-
bioRxiv - Genomics 2019Quote: ... Slides were washed three times with PBS and incubated with blocking solution (1% Bovine serum albumin, 10% Donkey serum, 0.3M Glycine, 0.1% PBS Tween) for 1 hour at room temperature followed by primary antibody in blocking solution overnight at 4 degrees (see Table 1 for source ...
-
bioRxiv - Physiology 2021Quote: ... the unweighted cart was attached to the mouse’s tail by interweaving a 10 cm long strip of vinyl tape (3M, #80610833842, Saint Paul, MN) around the tail and the nylon string of the cart (Fig ...
-
bioRxiv - Cell Biology 2023Quote: ... and incubated for 1 hour at room temperature in Blocking Solution (PBS containing 1% BSA, 10% FBS, 0.3M glycine and 0.1% Tween-20). The fixed cells were incubated with a mixture of the primary antibodies in PBS overnight at 4°C ...
-
bioRxiv - Genomics 2020Quote: ... Amplification products were purified using a standard protocol for DNA precipitation with sodium acetate and ethanol (1/10 3M sodium acetate, 2 v/v ethanol) (https://www.thermofisher.com/es/es/home/references/protocols/nucleic-acid-purification-and-analysis/dna-protocol/sodium-acetate-precipitation-of-small-nucleic-acids.html#comergent_product_list_15878) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were fixed with ice cold methanol for 5 min and then in blocking buffer (1% BSA, 10 % Horse serum, 0.3M Glycine, 0.2% Triton X in PBS) for 1 h RT ...
-
bioRxiv - Cell Biology 2023Quote: ... exchanged into a 1:1 volume mixture of 1X PBST and smiFISH Wash Buffer [10% 20X SSC (20X SSC: 0.3M sodium citrate, 3M NaCl, in nuclease-free H2O, pH7.0), 10% deionized formamide ...
-
bioRxiv - Biochemistry 2022Quote: ... dissolved in 200 mM NH4HCO2 (pH 10, 300 µL), then subsequently fractionated using a homemade stage tip (three layers of SDB-XC extraction disks, (3M, 1.07 mm x 0.50 mm i.d.)) in a 200 µL pipette tip ...
-
bioRxiv - Neuroscience 2023Quote: ... All wires were connected to a 10-pin socket used as a connector and firmly fixed to the skull with dental resin cement (56849, 3M Dental Products, St Paul, MN, USA). After surgery ...
-
bioRxiv - Immunology 2021Quote: ... Additional high stringency washes were performed after the secondary antibody and Opal fluor applications using high-salt TBST (0.05M Tris, 0.3M NaCl, and 0.1% Tween-20, pH 7.2-7.6). TCT was used as the blocking buffer (0.05M Tris ...