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Citations for 3M :
51 - 99 of 99 citations for Methacrylic acid N hydroxysuccinimide ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... wild-type male and female C57BL/6J mice (JAX; RRID:IMSR_JAX:000664) were used across ten experimental groups 30 s ITI (n = 6; 3F/3M), 60 s ITI (n = 19 ...
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bioRxiv - Cancer Biology 2024Quote: ... Formerly N-glycosylated peptides were desalted using C18-based solid phase extraction stage tips (3M™ Empore™), lyophilized in a SpeedVac vacuum concentrator ...
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bioRxiv - Immunology 2020Quote: ... was added and the reaction was stopped with the addition of acid solution (3M H3PO4). Absorbance (A450 nm ...
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bioRxiv - Cell Biology 2022Quote: ... The digested DNA was then transferred to a positively charged nylon membrane (Hybond, N+, Amersham, UK) overnight by capillary osmosis in presence of 20x SSC (3M NaCl, 0.3M sodium citrate tribasic dehydrate ...
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bioRxiv - Neuroscience 2024Quote: ... After insertion we sealed the gap between the cannula and the skull using n-butyl cyanoacrylate adhesive (Vetbond, 3M), before covering the area with dental cement (Metabon ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were acidified to 0.5% formic acid (FA) and desalted using home-made C18 (3M Empore) stage tips filled with 4 μl of Poros Oligo R3 resin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... we found that GB-O mice exhibited impaired recent LTM relative to mCh-O and young mice (GB-O; n=3M/3F ...
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bioRxiv - Cell Biology 2022Quote: ... The digested DNA was then transferred to a positively charged nylon membrane (Hybond, N+, Amersham, UK) overnight by capillary osmosis in presence of 20x SSC (3M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... mice (25-30g, n= 6-10 mice per group) were injected intraperitoneally with FA (F7876, Sigma-Aldrich, dissolved in 0.3M sodium bicarbonate at a dose of 250 mg/kg) ...
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bioRxiv - Immunology 2020Quote: ... TMB substrate was added and the reaction was stopped by the addition of acid solution (3M H3PO4). Absorbance was measured by Synergy Mx microtiter plate reader (Biotek).
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bioRxiv - Microbiology 2024Quote: ... Trifluoroacetic acid was added to halt digestion and the samples were desalted using C18 resin (Empore, 3M) before HPLC-MS analysis.
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bioRxiv - Developmental Biology 2021Quote: ... while expression in the eyes was undetected (f8a/c6996; Fig.2-figure supplement 3K,L) or extremely weak (dmd/c7924; Fig.2-figure supplement 3M,N). Along with our set of control genes ...
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bioRxiv - Plant Biology 2021Quote: ... 0.1% formic acid (FA) and desalted on self-packed StageTips (five disks, Ø 1.5 mm C18 material, 3M EmporeTM ...
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bioRxiv - Genetics 2022Quote: Dot blot membranes were rinsed in 2X SSC (20x: 3M NaCl and 0.3M citric acid trisodium salt dihydrate) and pre-hybridized for 15’ with Tetramethylammonium Chloride (TMAC ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The tryptic peptides were acidified by trifluoroacetic acid and desalted by SDB-XC StageTips (catalog no. 2340; 3M). The desalted samples were dried by a vacuum centrifugation concentrator ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested peptides were desalted with 60% acetonitrile/0.3% formic acid through the three disks of C-18 Empore SPE disks (3M) in a 200 μl pipet tip ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were desalted with stage tips using the following procedure: Peptides were reconstituted with 5% acetonitrile/0.1 % formic acid and loaded onto Empore C18 disks (3M) packed into a 200 µl pipette tips pre-equilibrated with LC-MS grade methanol and water containing 0.1% formic acid ...
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bioRxiv - Biophysics 2023Quote: ... Half of each samples was acidified (0.05% formic acid) followed by desalting and concentration by micro solid phase extracted (Empore, 3M)48 ...
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bioRxiv - Cell Biology 2022Quote: ... The gels were blotted to Amersham Hybond-N membranes (GE) via overnight capillary transfer with 20x SSC (3M NaCl, 0.3 M sodium citrate). The membranes were crosslinked under UV at 1200×100 μJ/cm2 and then incubated in pre-hybridization buffer (1.5x SSC ...
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bioRxiv - Developmental Biology 2020Quote: ... of labeled peptides were then mixed with 0.1% trifluoroacetic acid (TFA) (Pierce) to generate a test mix which was desalted by StageTip fractionation with Empore C18 filters (3M) (Rappsilber et al. ...
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bioRxiv - Neuroscience 2021Quote: ... layer [35] glued to a custom 3D-printed polylactic acid frame was implanted on the exposed brain and secured to the skull using VetBond (3M) and dental cement ...
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bioRxiv - Microbiology 2021Quote: ... Digested samples were acidified to a final concentration of 0.5% formic acid and desalted with home-made high-capacity StageTips composed of 1 mg Empore™ C18 material (3M) and 5 mg of OLIGO R3 reverse phase resin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Digested samples were acidified to a final concentration of 0.5% formic acid and desalted with home-made high-capacity StageTips composed of 1 mg Empore™ C18 material (3M) and 5 mg of OLIGO R3 reverse phase resin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Digested peptides were acidified with 10% formic acid (FA) to pH ∼2 and desalted using stage tips with Empore C18 SPE Extraction Disks (3M) and dried under vacuum.
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bioRxiv - Immunology 2020Quote: ... Labeled samples were acidified using triflouroacetic acid (TFA) and cleaned-up using StageTip (3-disc plug of C18 Empore material, 3M). StageTip’s were conditioned by rinsing them with (2x ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were desalted using stage-tips containing a Poly-styrene-divinylbenzene copolymer modified with sulfonic acid groups (SDB-RPS; 3M) material ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Digested peptides were acidified with 10% formic acid to pH ~2 and desalted using stage tips with Empore C18 SPE Extraction Disks (3M) and dried under vacuum.
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bioRxiv - Cell Biology 2023Quote: ... membranes were briefly washed twice with wash buffer (0.1M Maleic acid, 3M NaCl; 0.1% Tween 20 adjusted to pH 7.5), before blocking for 30 minutes and probing with a DIG antibody for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... samples were acidified to 0.3% formic acid with 10% formic acid and cleaned up with an MCX STAGE tip (39) using Empore SDB-RPS extraction disks material from 3M. The eluted samples were Speedvaced to dryness.
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixtures were collected using a magnetic rack, acidified with Buffer A* (0.1% trifluoroacetic acid, 2% acetonitrile) and desalted using C18 StageTips (Empore™, 3M) with the addition of Oligo-R3 resin reverse phase material (Thermo ...
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bioRxiv - Physiology 2024Quote: ... Peptides were desalted using stage-tips containing a poly-styrene-divinylbenzene copolymer modified with sulfonic acid groups (SDB-RPS; 3M) material ...
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bioRxiv - Cell Biology 2022Quote: ... Each sample was acidified in formic acid and subjected to SPE on an Empore SD C18 plate (3M catalogue# 6015 SD). Each sample was lyophilized and reconstituted in 140mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Digests were acidified by addition of 0.5% (v/v) formic acid (FA) and desalted using self-packed StageTips (three disks per micro-column, ø 1.5mm, C18 material, 3M Empore). The peptide eluates were dried to completeness and stored at −80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... formic acid (FA) and desalted using self-packed StageTips (five disks per micro-column, ø 1.5 mm, C18 material, 3M Empore). The peptide eluates were dried to completeness and stored at −80 °C ...
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bioRxiv - Immunology 2023Quote: ... or the germline-targeting BG505 SOSIP.v4.1-GT1.1 (abbreviated to BG505 GT1.1) trimer (n=5/group) with 10µg 3M-052 adjuvant in a 2% squalene emulsion (3M-052-SE; provided by AAHI and 3M) at 0 ...
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bioRxiv - Developmental Biology 2022Quote: ... Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Genetics 2021Quote: ... Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Cancer Biology 2020Quote: ... Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Developmental Biology 2022Quote: ... Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Molecular Biology 2023Quote: ... Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Developmental Biology 2023Quote: ... Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Cancer Biology 2021Quote: ... 1000 ng and 500 ng of each RNA sample were spotted to a nylon membrane (Hybond N, Dutscher RPN203B) previously soaked in 2X SSPE solution (0.3M NaCl, 20mM sodium phosphate, 2 mM EDTA) and inserted in the dot blot apparatus (SCIE-PLAS) ...
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bioRxiv - Neuroscience 2021Quote: ... https://www.mathworks.com/matlabcentral/fileexchange/51986-perceptually-uniform-colormaps To compute swim-triggered averages and for analyses correlating neuronal activity to different features of swim kinematics (Figure 3M-N, S2E-G and S2N-P), only ‘unitary’ bouts were taken into account ...
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bioRxiv - Neuroscience 2021Quote: ... after trypsin digestion ach sample was acidified in formic acid and subjected to SPE on an Empore SD C18 plate (3M catalogue# 6015 SD). Each sample was lyophilized and reconstituted in 140mM HEPES ...
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bioRxiv - Developmental Biology 2020Quote: ... peptides were desalted with C18 columns and reconstituted in 1% formic acid in water and further separated into five fractions by strong cation exchange chromatography (SCX, 3M Purification, Meriden, CT). Eluates were first dried in a SpeedVac ...
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bioRxiv - Biochemistry 2020Quote: ... The exchange was quenched for 2 min at 1°C with an equal volume of quench buffer (3M guanidine HCl, 0.1% formic acid). Proteins were cleaved with pepsin and separated by reverse-phase chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... after trypsin digestion ach sample was acidified in formic acid and subjected to SPE on an Empore SD C18 plate (3M catalogue# 6015 SD). Samples were dried out/lyophilized and reconstituted in 140 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... BrdU detection required formic acid pre-treatment (formamide 50% in 2× SSC buffer; 2× SSC is 0.3M NaCl and 0.03 M sodium citrate, pH 7.0) at 65 °C for 2 h followed by DNA denaturation for 30 min in 2 M HCl for 30 min at 37°C and rinsed in 0.1 M borate buffer pH 8.5 for 10 min ...
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bioRxiv - Genomics 2021Quote: ... RNAs were isolated by acid phenol: chloroform and precipitated in 1 ml Ethanol supplemented with (supplemented with 50 µL 3M NaOAc pH 5.2, 1 µl of glycoblue). Pellets were resuspended in 20 ul DEPC-treated H2O ...