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Citations for 3M :
51 - 100 of 131 citations for Dioctanoyl phosphatidic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... purified RNA samples were precipitated using sodium acetate (3M, 0.1x sample volume) and ethanol (100%,3x sample volume) ...
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bioRxiv - Microbiology 2021Quote: ... Replicate DNA extractions were pooled and concentrated using sodium acetate (0.3M final concentration) and ethanol (2x volume ...
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bioRxiv - Genetics 2021Quote: ... Two chloroform:isoamylalcohol washes were then performed followed by sodium acetate precipitation (3M, pH4.8), and one wash of 70% ethanol before DNA extracts were suspended in ultrapure H20 and stored at −20°C.
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed twice with 2xSSC (3M NaCl, 300 mM sodium citrate 2H2O) + 0.1% SDS and bound probe was detected using a FLA-5000 PhosphoImager (Fuji).
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bioRxiv - Physiology 2024Quote: ... the pellet was resuspended in denaturing buffer (5M guanidine hydrochloride, 0.3M sodium sulfite) and treated with S-sulfonating agent ...
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bioRxiv - Biochemistry 2024Quote: ... protein was eluted with 10 ml of elution buffer E1-E6 [lysis buffer having different concentration of salt (E1=0.3M, E2=0.4M ...
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bioRxiv - Biochemistry 2021Quote: ... The pH of eluted antibodies was immediately neutralized using sodium acetate (3M, pH 9.0). After protein measurements at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... by capillary action in 20x SSC (3M NaCl, 0.3 M sodium citrate pH 7.0) overnight ...
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bioRxiv - Microbiology 2023Quote: ... The DNA was precipitated by addition of 1/10 volume of sodium acetate (3M), 1 uL of glycogen ...
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bioRxiv - Bioengineering 2023Quote: ... the biodome was then placed in an acid bath (3M HCl) overnight (≥ 10 hours) ...
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bioRxiv - Microbiology 2020Quote: ... followed by another wash in 20X SSC buffer (3M NaCl, 0.3M sodium citrate, pH 7.0) for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... followed by another wash in 20X SSC buffer (3M NaCl, 0.3M sodium citrate, pH 7.0) for 15 min ...
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bioRxiv - Immunology 2021Quote: The slides were equilibrated with 300μl buffer 2xSSC (0.3M NaCl, 0.03M sodium citrate, pH=7.0) 2x 3mins ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was precipitated by adding 0.1 volume of sodium acetate (NaOAc, 3M, pH 5.2) and 2.5 volumes of absolute ethanol ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were incubated in 2x SSC-T (3.0M NaCl, 0.3M Sodium Citrate, 0.1% Tween 20) for 5 minutes at RT ...
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bioRxiv - Biochemistry 2023Quote: ... and 200 U/L of ACE in 0.1M sodium borate buffer (pH 8.3, 0.3M NaCl). Six inhibitor concentrations were tested (5 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we added 17.5 μL sodium acetate (pH 5.2 3M; Alfa Aesar, Ward Hill, MA, USA), 1 μL glycogen ...
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bioRxiv - Immunology 2024Quote: ... was diluted in water with increasing salt concentrations (titration of 1.8M, 2M, 2.2M, 2.4M, 2.6M, 2.8M, 3M, 3.2M, 3.4M, 3.6M and 4M NaCl) to induce Z-DNA conformation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were re-precipitated using a standard ethanol precipitation protocol with sodium acetate (3M, pH 5.2) as the carrier molecule ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1 mg/ml of RNase A in 2x SSC (0.3M NaCl, 30 nM sodium citrate, pH 7.0) was added onto the coverslips and incubated at 37°C for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Isopropanol (4°C, 1:1 volume ratio) and sodium acetate (4°C, 3M, 1:10 volume ratio) were added to the mixture prior to precipitation (−20°C ...
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bioRxiv - Molecular Biology 2024Quote: Precipitate RNA with 2X volume Molecular Grade Absolute ethanol and 0.1X volume Sodium Acetate (3M, pH 5.2). Incubate the samples for 1 hour at -20ºC and centrifuge at 14,000 g for 15 minutes at 4ºC.
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bioRxiv - Biochemistry 2021Quote: ... Fatty acids from total lipids were released by saponification (refluxing with 3mL 0.3M KOH in 90% methanol at 80°C for 1 hour and remaining ∼300μL solvent) ...
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bioRxiv - Neuroscience 2022Quote: ... neural and behavioral data were collected—20min of “passive” recording followed by 120 taste delivery trials in which one of four gustatory stimuli (0.1M sodium chloride [NaCl], 0.3M Sucrose ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was precipitated with 2X volume Molecular Grade Absolute ethanol and 0.1X volume Sodium Acetate (3M, pH 5.2). The samples were then incubated for 1 hour at -20°C and centrifuged at 14,000 g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 150 μL of freshly made bleaching solution (50 µL deionized formamide, 50 µL of 3M sodium chloride/0.3M sodium citrate ...
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bioRxiv - Developmental Biology 2020Quote: ... the probes were removed by four washes in increasing dilutions of 2 X SSC (NaCl 0.3M; Sodium citrate 0.03M) at 70°C (25 ...
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bioRxiv - Molecular Biology 2023Quote: ... The oligos were then ethanol precipitated by addition of 4 μL sodium acetate (3M, pH5.2, AMRESCO, Cat.E521-100 ml) and 110 μL 100% ethanol and cooled to −80c for at least 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... EasySep Streptavidin magnetic beads (StemCell) were washed in 100µL 6X SSC (For 20X SSC: 3M NaCl, 0.3M sodium citrate) 3 times before incubation overnight on a rotisserie at 4°C in blocking buffer (2% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... EasySep Streptavidin magnetic beads (StemCell) were washed in 100µL 6X SSC (For 20X SSC: 3M NaCl, 0.3M sodium citrate) 3 times before incubation overnight on a rotisserie at 4°C in blocking buffer (2% BSA ...
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bioRxiv - Genomics 2024Quote: ... DNA extractions were performed by combining 150 µl of demineralized material with 1.5 ml binding buffer (500 ml Qiagen PB, supplemented with 15 ml Sodium acetate 3M, and 1.25 ml 5M NaCl ...
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bioRxiv - Immunology 2020Quote: ... was added and the reaction was stopped with the addition of acid solution (3M H3PO4). Absorbance (A450 nm ...
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bioRxiv - Immunology 2021Quote: ... Additional high stringency washes were performed after the secondary antibody and Opal fluor applications using high-salt TBST (0.05M Tris, 0.3M NaCl, and 0.1% Tween-20, pH 7.2-7.6). TCT was used as the blocking buffer (0.05M Tris ...
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bioRxiv - Microbiology 2020Quote: ... The protocol used for DNA precipitation included the following steps: for each sample a 1/10 volume of sodium - acetate (3M) was added (pH=5.2) ...
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bioRxiv - Microbiology 2023Quote: ... 50μg of each plasmid per transfection was ethanol precipitated overnight (2.5 volumes 100% ethanol, 1/10 volumes sodium acetate (3M, pH5.2), - 20°C) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were acidified to 0.5% formic acid (FA) and desalted using home-made C18 (3M Empore) stage tips filled with 4 μl of Poros Oligo R3 resin (Thermo Fisher) ...
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bioRxiv - Genetics 2021Quote: ... then rinsed and incubated in two changes of wash buffer C (10% formamide, 2XSSC [0.3M NaCL, 30 mM sodium citrate (7.0)] ...
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bioRxiv - Synthetic Biology 2024Quote: ... all ten reactions of double-digested pR-fp fragments were pooled into a 2mL-DNA LoBind Eppendorf tube and supplemented with 1/10 v/v of sodium acetate (3M, pH5.2) (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... TMB substrate was added and the reaction was stopped by the addition of acid solution (3M H3PO4). Absorbance was measured by Synergy Mx microtiter plate reader (Biotek).
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bioRxiv - Microbiology 2024Quote: ... Trifluoroacetic acid was added to halt digestion and the samples were desalted using C18 resin (Empore, 3M) before HPLC-MS analysis.
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bioRxiv - Microbiology 2021Quote: ... Gaussia luciferase activity was assessed 48 hrs after infection by combining cell culture media with Gaussia luciferase substrate (0.1M Tris pH 7.4, 0.3M sodium ascorbate, 10 μM coelenterazine). Luminescence was immediately measured by a BioTek Cytation5 plate reader.
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed with a series of HYB- (50% formamide, 5X SSC with 0.1% Tween) mixed into 2X SSC (0.3M NaCl, 30mM sodium citrate) (75% ...
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bioRxiv - Genomics 2024Quote: ... Beads were washed 4 times with 2x SSC buffer containing 0.1% NP40 (0.3M of NaCl, 0.3M of Sodium citrate, 0.1% NP-40) at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... 0.1% formic acid (FA) and desalted on self-packed StageTips (five disks, Ø 1.5 mm C18 material, 3M EmporeTM ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The tryptic peptides were acidified by trifluoroacetic acid and desalted by SDB-XC StageTips (catalog no. 2340; 3M). The desalted samples were dried by a vacuum centrifugation concentrator ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... The RNA was eluted in 400 μL RNA elution buffer (EB) at 4°C overnigt (RNA EB: 0.3M sodium acetate (NaOAc, pH 5.5), 1 mM EDTA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The isolated supernatant was mixed with 1500 μL pre-chilled absolute ethanol (Concord Technology, Tianjin) and 50 μL sodium acetate solution (3M, Beijing Chemical Works), kept at −20 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The gels were blotted to Amersham Hybond-N membranes (GE) via overnight capillary transfer with 20x SSC (3M NaCl, 0.3 M sodium citrate). The membranes were crosslinked under UV at 1200×100 μJ/cm2 and then incubated in pre-hybridization buffer (1.5x SSC ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested peptides were desalted with 60% acetonitrile/0.3% formic acid through the three disks of C-18 Empore SPE disks (3M) in a 200 μl pipet tip ...
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bioRxiv - Biophysics 2023Quote: ... Half of each samples was acidified (0.05% formic acid) followed by desalting and concentration by micro solid phase extracted (Empore, 3M)48 ...