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Citations for Expression Systems :
201 - 250 of 308 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
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bioRxiv - Cell Biology 2023Quote: Retriever was expressed by co-infecting Sf9 cells at 2 M/mL cell density (Expression Systems) with individual baculoviruses prepared by the Bac-to-Bac system used in other studies58 ...
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bioRxiv - Cell Biology 2023Quote: ... BestBac 2.0 Δ v-cath/chiA Linearized Baculovirus DNA was obtained from Expression Systems (91-002).
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bioRxiv - Biochemistry 2023Quote: ... the culture was supplemented with 10 mL (5%) Boost Production Additive (Expression Systems LLC, Davis, CA) where indicated ...
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bioRxiv - Cell Biology 2023Quote: ... frugiperda insect cells grown at 27°C in ESF 921 Insect Cell Culture Medium (Expression Systems) supplemented with Fetal Bovine Serum (2% final concentration) ...
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bioRxiv - Cell Biology 2023Quote: ... frugiperda insect cells grown at 27°C in ESF 921 Insect Cell Culture Medium (Expression Systems) supplemented with Fetal Bovine Serum (2% final concentration) ...
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bioRxiv - Biochemistry 2023Quote: Mouse CRY1 PHR domain (residues 1-491) was expressed in Sf9 suspension insect cells (Expression Systems) as previously described (Parico et al. ...
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bioRxiv - Immunology 2024Quote: ... Stable polyclonal S2 insect cells lines were generated through transient transfection with ExpreS2TR reagent (Expression Systems) mixed with the relevant plasmid and subsequent culturing under selection with G418 (Gibco ...
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bioRxiv - Genetics 2021Quote: ... were transfected using PEI transfection methods and cultured in protein-free ESF921 media (Expression Systems, 96-001). For 6-well plate transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Titers were determined by flow cytometric analysis staining P1 infected cells with gp64-PE antibody (Expression Systems). Expression of AE1 for structural studies was carried out by infection of Sf9 cells at a cell density of 2-3 ×106 cells/ml with P1 virus at MOI (multiplicity of infection ...
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bioRxiv - Biophysics 2021Quote: ... and scFv16 were co-expressed in Trichoplusia ni Hi5 insect cells using the baculovirus method (Expression Systems). Cell cultures were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Biophysics 2021Quote: ... and Gγ2 were co-expressed in Trichoplusia ni Hi5 insect cells using the baculovirus method (Expression Systems). Cell cultures were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Biophysics 2022Quote: ... P0 virus was produced by transfection with FuGENE HD and BestBac 1.0 Linearized Baculovirus DNA (Expression Systems) followed by a viral amplification to P1 ...
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bioRxiv - Biophysics 2019Quote: ... 13 mL of the second amplification was used to infect 1 L of Sf21 cells (Expression Systems) at 2.8 × 106 cells/mL and incubated at 27°C for 48 hours ...
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bioRxiv - Biophysics 2019Quote: ... 1 microgram of bacmid DNA was diluted in ESF 921 media (Expression systems, no antibiotic or serum), mixed with 6 μL of Fugene HD transfection reagent (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... were cultured on a shaking incubator at 27°C and 120 rpm in ESF921 medium (Expression Systems).
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were obtained from Expression Systems and maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... was subsequently produced from pl397 according to the manufacturer’s instructions (Bac-to-Bac Baculovirus Expression system, Invitrogen). The pl485 plasmid (pFastBac-MBP-TOPOVIBLΔC25K281EK282E ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... This CB1R construct was expressed in Spodoptera frugiperda Sf9 insect cells with the baculovirus method (Expression Systems). Insect cell pellets expressing CB1R was solubilized with buffer containing 1% lauryl maltose neopentyl glycol (L-MNG ...
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bioRxiv - Biochemistry 2024Quote: ... Spodoptera frugiperda Sf9 cells were transfected with specific plasmid and linearized baculovirus DNA (Expression Systems, 91-002) using Cellfectin II (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... All plasmids were co-transfected with linearized 2.0 DNA (deficient in v-cath/chiAgenes) (Expression Systems, CA, USA) into Sf9 insect cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi5 cells grown in ESF-921 media (Expression Systems, Davis, CA, United States) at 27 °C ...
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bioRxiv - Biochemistry 2022Quote: DHX9-His was expressed by transducing 800 ml Tni cell culture in ESF921 serum-free media (Expression Systems) at a density of 1×106 cells/ml with 16 ml baculoviral suspension (generated in Sf9 cells ...
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bioRxiv - Cell Biology 2020Quote: ... and in adherent culture on T-25 tissue culture flasks in Grace’s media (Gemini Bio-Products; Expression systems) with 10% FBS (Gemini Bio-Products ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... were grown to a density of 4x106 cells/mL in ESP 921 media (Expression Systems, 96- 001-01) at 27 °C ...
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bioRxiv - Biophysics 2023Quote: ... were grown at 27 °C and 100 rpm agitation in ESF921™ insect cell-culture medium (Expression Systems), keeping cell density between 0.4 - 2.5 × 106 cells/ml ...
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bioRxiv - Biochemistry 2023Quote: Co-expression of MeSufDSUC and MeSufDSUBC was performed in Spodoptera frugiperda ExpiSf9 cells (ExpiSf™ Expression System, ThermoFisher) in ExpiSfCD media through infection with baculovirus according to manufacturer’s instructions (Bac-to-Bac™ Baculovirus Expression system ...
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bioRxiv - Biophysics 2023Quote: ... and the P3 virus was used to infect Tni suspension insect cell culture (Expression Systems; Cat. 94-002S) for protein expression.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Approximate titers were estimated by flow cytometric analysis staining P1 infected cells with gp64-PE antibody (Expression Systems). All protein for this study ...
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bioRxiv - Biochemistry 2020Quote: ... Baculovirus was generated using Spodoptera frugiperda Sf9 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems 94-001F) and the construct was expressed in Spodoptera frugiperda Sf9 insect cells ...
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bioRxiv - Immunology 2022Quote: ... The plate was left to incubate for 6 hours followed by addition of the ESF-AF medium (Expression systems). Sf9 cells were left for seven days at 27 °C without agitation ...
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bioRxiv - Immunology 2022Quote: ... 2 μg of each plasmid was transfected into SF9 insect cells with BestBac 2.0 linearized baculovirus DNA (Expression Systems) using Cellfectin II reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Spodoptera frugiperda ovarian-derived Sf9 cells were maintained in suspension culture in ESF 921 media (Expression Systems, Davis, CA) and in adherent culture in Grace’s media (Gemini Bio-Products ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Biochemistry 2023Quote: ... in ExpiSfCD media through infection with baculovirus according to manufacturer’s instructions (Bac-to-Bac™ Baculovirus Expression system, Gibco). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: Baculovirus encoding Halo-Ku80/Ku70 was amplified in three stages (P1, P2, and P3) in Sf9 cells (Expression Systems). To express X ...
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bioRxiv - Biophysics 2020Quote: ... 1μg of recombinant bacmid DNA was combined in 1 mL of ESF-921 media (Expression Systems, Cat. #96-001-01) with 20 μL of Cellfectin II (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell cultures were grown to a density of 4×106 cells/ mL in ESF 921 serum-free medium (Expression Systems). For the expression of the DRD1-Gs complexes ...
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bioRxiv - Immunology 2021Quote: ... GenBank: MN908947.3) under the control of a tetracycline-inducible promoter (Retro-X™ Tet-One™ Inducible Expression System, Takarabio) with a puromycin selection marker ...
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bioRxiv - Biochemistry 2021Quote: ... Protein expression was induced by baculovirus infection of 6-8 liter cultures of Sf9 cells in ESF921 medium (Expression Systems) at a density of ~2 × 106 cells/ml ...
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bioRxiv - Microbiology 2020Quote: ... as described in the protocol of the BestBactm 2.0 Δ v-cath/ChiA Baculovirus Cotransfection Kit (Expression Systems, CA, USA). P1 baculovirus was generated with suspended Sf9 cells (2 ×106 cells/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... The wild-type BTK construct was cloned in the base vector (Lenti-X Expression System Version EF1-a; Takara Bio). STable over-expression of BTK in ibrutinib-resistant DLBCL cells was performed by using lentiviruses expressing human wild-type BTK as described previously.[15]
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bioRxiv - Molecular Biology 2020Quote: ... were seeded per well in a 6-well plate in protein-free ESF 921 media (Expression Systems Cat# 96-001) with no additives or antibiotics ...
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bioRxiv - Molecular Biology 2021Quote: ... GIPR and Gs heterotrimer were co-expressed in High Five™ cells grown in ESF 921 culture medium (Expression Systems) to a density of 3.2 × 106 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... Plasmids for each chain were separately transfected into SF9 insect cells with BestBac 2.0 baculovirus DNA (Expression Systems; Davis, CA) and Cellfectin II reagent (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
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bioRxiv - Immunology 2022Quote: ... 2 μg of plasmid DNA was transfected into SF9 insect cells using BestBac 2.0 baculovirus DNA (Expression Systems; Davis, CA) and Cellfectin II reagent (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... When the cells grew to a density of 3.5 × 106 cells/mL in ESF 921 cell culture medium (Expression Systems), we infected the cells with five separate virus preparations at a ratio of 1:2:2:2:2 for PTH1R(27-502)-LgBiT-2MBP ...
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bioRxiv - Biochemistry 2022Quote: ... Spodoptera frugiperda Sf9 insect cells were co-transfected with the constructed plasmids and linearized baculovirus DNA (Expression Systems, 91-002) using Cellfectin II (Thermo Fisher ...