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Citations for Expression Systems :
51 - 100 of 223 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Trichoplusia ni Hi5 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems) were infected at a density of 3.0 x 106 cells/mL with high titer Gβ1γ2-baculovirus ...
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bioRxiv - Biophysics 2023Quote: ... recombinant FUS was expressed in Sf9 insect cells (Expression Systems, Cat#94-001F) for 72 h using the baculovirus expression system87 and produced as a C-terminal SNAP fusion with an N-terminal maltose-binding protein (MBP ...
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bioRxiv - Biophysics 2022Quote: ... for 60-72 hrs maintained in ESF 921 Insect Cell Culture Medium (Expression Systems) at 27°C with ambient CO2 and gentle agitation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Trichoplusia ni (T. ni) insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems) were co-infected with two baculoviruses at a density of ∼3.0 × 106 cells per ml ...
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bioRxiv - Biochemistry 2022Quote: ... in Spodoptera frugiperda Sf9 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems). For expression ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 and insect cell culture media were purchased from Expression Systems (Davis, CA, USA). Fetal bovine serum was purchased from PEAK (Wellington ...
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bioRxiv - Biochemistry 2021Quote: ... insect cells plated for 24 hours in a Corning 6-well plastic dish (Cat# 07-200-80) containing 2 mL of ESF 921 Serum-Free Insect Cell Culture media (Expression Systems, Cat# 96-001, Davis, CA.). All media contains 1x concentration of Antibiotic-Antimycotic (Gibco/Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... insect cells plated for 24 hours in a Corning 6-well plastic dish (Cat# 07-200-80) containing 2 mL of ESF 921 Serum-Free Insect Cell Culture media (Expression Systems, Cat# 96-001, Davis, CA.). All media contains 1x concentration of Antibiotic-Antimycotic (Gibco/Invitrogen ...
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bioRxiv - Biophysics 2021Quote: Baculovirus expressing SSTR2KappaICL3-GFP at P2 was used to infect Sf9 insect cells (Expression Systems) at a density of 3–4 million cells/ml and incubated at 28C with shaking ...
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bioRxiv - Biochemistry 2019Quote: ... or GST-tagged BMAL1 PAS-AB were expressed in Sf9 suspension insect cells (Expression Systems). Sf9 suspension cells were infected with a P3 virus at 1.2 × 106 cells per milliliter and grown for 72 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Trichoplusia ni (T. ni) insect cells using baculoviruses generated by the BestBac method (Expression Systems) were used for expression of Gs heterotrimer ...
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bioRxiv - Immunology 2021Quote: ... The protein was expressed in 2 L of Sf9 cells at 2×106 cells/mL maintained in ESF921 media (Expression Systems) by adding 25 mL virus per liter of culture ...
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bioRxiv - Biochemistry 2023Quote: Mouse CRY1 PHR domain (residues 1-491) was expressed in Sf9 suspension insect cells (Expression Systems) as previously described (Parico et al. ...
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bioRxiv - Immunology 2024Quote: ... Stable polyclonal S2 insect cells lines were generated through transient transfection with ExpreS2TR reagent (Expression Systems) mixed with the relevant plasmid and subsequent culturing under selection with G418 (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... and scFv16 were co-expressed in Trichoplusia ni Hi5 insect cells using the baculovirus method (Expression Systems). Cell cultures were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Biophysics 2021Quote: ... and Gγ2 were co-expressed in Trichoplusia ni Hi5 insect cells using the baculovirus method (Expression Systems). Cell cultures were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... This CB1R construct was expressed in Spodoptera frugiperda Sf9 insect cells with the baculovirus method (Expression Systems). Insect cell pellets expressing CB1R was solubilized with buffer containing 1% lauryl maltose neopentyl glycol (L-MNG ...
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bioRxiv - Biochemistry 2021Quote: ... Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems). Next ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The Tni insect cells were grown to a density of 4 million cells/ml in ESF 921 serum free media (Expression systems) before infection with a 4:2:1 ratio of CCK1R:DNGαS:Gβ1γ2 baculoviruses ...
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bioRxiv - Biophysics 2023Quote: ... Bacmid DNA was then used for lipofection of Sf9 insect cells grown in serum-free ESF921 cell culture medium (Expression Systems) at 27 °C using ESCORT IV reagent (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... were grown at 27 °C and 100 rpm agitation in ESF921™ insect cell-culture medium (Expression Systems), keeping cell density between 0.4 - 2.5 × 106 cells/ml ...
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bioRxiv - Biochemistry 2021Quote: Spodoptera frugiperda Sf9 cells were maintained as suspension cultures at 27 °C in ESF-921™ Insect Cell Culture Medium (Expression Systems). Isolation of bacmid DNA ...
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bioRxiv - Bioengineering 2020Quote: The RBD (residues 319-541) of the SARS-Cov-2 S protein was expressed as a secreted protein in Spodoptera frugiperda Sf9 cells (Expression Systems) using the Bac-to-bac baculovirus method (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Baculovirus was generated using Spodoptera frugiperda Sf9 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems 94-001F) and the construct was expressed in Spodoptera frugiperda Sf9 insect cells ...
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bioRxiv - Biochemistry 2023Quote: ... Infected cells were typically grown for 48 hours at 27ºC in ESF 921 Serum-Free Insect Cell Culture medium (Expression Systems, Cat# 96-001-01). Cells were harvested by centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Plasmids for each chain were separately transfected into SF9 insect cells with BestBac 2.0 baculovirus DNA (Expression Systems; Davis, CA) and Cellfectin II reagent (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... Spodoptera frugiperda Sf9 insect cells were co-transfected with the constructed plasmids and linearized baculovirus DNA (Expression Systems, 91-002) using Cellfectin II (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems), followed by purification through Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... Infected cells were typically grown for 48 hours at 27°C in ESF 921 Serum-Free Insect Cell Culture medium (Expression Systems, Cat# 96-001-01). Cells are harvested by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were typically grown for 48 hours at 27°C in ESF 921 Serum-Free Insect Cell Culture medium (Expression Systems, Cat# 96-001-01) and then harvested by centrifugation ...
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bioRxiv - Biophysics 2019Quote: ... with N-terminal FLAG and C-terminal octahistidine tag was expressed in Spodoptera frugiperda Sf9 insect cells using the baculovirus method (Expression Systems) in the presence of the L-168,049 (Tocris Bioscience) ...
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bioRxiv - Biophysics 2019Quote: ... heterotrimeric Gs was expressed in High Five™ (Trichuplusia ni) insect cells using baculoviruses generated by the BestBac method (Expression Systems). Two separate baculoviruses were used ...
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bioRxiv - Cell Biology 2021Quote: His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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bioRxiv - Biochemistry 2021Quote: ... Both wild-type and kinase-dead (D286A) Sgk3 were expressed in baculvirus-infected Sf9 insect cells grown in ESF 921 medium (Expression Systems) at 27°C ...
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bioRxiv - Bioengineering 2022Quote: ... All baculovirus-based constructs in this work were expressed for 48 hours by infecting HiFive Trichoplusia ni insect cell cultures (Expression Systems) at a density of 2×106 cells/mL with infective baculovirus particles ...
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bioRxiv - Biochemistry 2022Quote: ... human full-length CB1 containing a N-terminal FLAG tag and C-terminal histidine tag was expressed in Spodoptera frugiperda Sf9 insect cells with the baculovirus method (Expression Systems). Receptor was extracted using 1% lauryl maltose neopentyl glycol (L-MNG ...
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bioRxiv - Biochemistry 2023Quote: ... The human heterodimeric Gβ1γ2 was expressed in Spodoptera frugiperda Sf9 insect cells and the anti-Bril Fab was expressed in secreted form from Trichuplusia ni Hi5 insect cells using the baculovirus method (Expression Systems), respectively ...
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bioRxiv - Biochemistry 2024Quote: The HormR+GAIN/7TM construct of ADGRL3 was expressed in Sf9 insect cells grown in ESF 921 serum-free media (Expression System). Cells grown to a density of 4.0 × 106 cells ml−1 were infected with high-titer baculovirus ...
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bioRxiv - Biochemistry 2023Quote: ... or 2% vol/vol PI3Kγ (PIK3CG/PIK3R5) baculovirus and grown at 27ºC in ESF 921 Serum-Free Insect Cell Culture media (Expression Systems, Cat# 96-001) for 48 hours ...
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bioRxiv - Cell Biology 2023Quote: For expression of Nv-Osk and Nv-Vas proteins in S2 (Drosophila Schneider 2) cells (Expression Systems, cat # 94-005S), corresponding cDNAs were subcloned under control of Actin5C promoter (GenScript) ...
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bioRxiv - Biochemistry 2020Quote: ... which was amplified once to give a V1 virus stock used to infect 1 l cultures of Sf9 cells (Expression Systems). Cells were grown in ESF 921 Insect Cell Culture Medium Protein Free (expression systems 96-001-01 ...
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bioRxiv - Biochemistry 2021Quote: ... The virus titers were measured by flow cytometry assay (Expression Systems).
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bioRxiv - Biophysics 2021Quote: ... and the virus was prepared using the BestBac system (Expression System, LLC). N-terminal Flag-tagged human Gβ1 ...
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bioRxiv - Biochemistry 2022Quote: ... and protein was expressed in Sf9 cells (Expression Systems) using the Bac-to-Bac Baculovirus expression system (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2×106 Sf9 cells (Expression Systems Cat# 94-001S) were seeded per well in a 6-well plate in protein-free ESF 921 media (Expression Systems Cat# 96-001 ...
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bioRxiv - Biochemistry 2022Quote: Drosophila Schneider 2 (S2) cells (Expression Systems, 94-005F) were cultured in ESF media (ESF 921 Insect Cell Culture Medium ...
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bioRxiv - Biophysics 2022Quote: ... The protein was expressed in Trichoplusia ni ovarian cells (Expression Systems) for 60-72 hrs maintained in ESF 921 Insect Cell Culture Medium (Expression Systems ...
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bioRxiv - Biophysics 2024Quote: ... The virus titer was determined by gp64-PE mouse anti-baculovirus antibody (Expression Systems, CA) using a Guava benchtop Flow Cytometer (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... Sf9 cells were seeded at 2 x 106 cells / mL in ESF-921 (Expression Systems), and infected simultaneously with myosin-15 baculovirus at a multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were expressed in Trichoplusia ni High Five cells (Expression Systems) by baculoviral expression ...