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Citations for Molecular Dimensions :
51 - 100 of 100 citations for Protein Crystallization since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Crystallization assays were carried out using the hanging drop vapor diffusion method in 48-well plates (Molecular Dimensions) at 20°C ...
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bioRxiv - Biochemistry 2020Quote: ... All crystallization experiments were setup using an NT8 drop-setting robot (Formulatrix Inc.) and UVXPO MRC (Molecular Dimensions) sitting drop vapor diffusion plates at 18°C ...
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bioRxiv - Microbiology 2022Quote: ... These were cryoprotected in the crystallization solution supplemented with 20% (v/v) glycerol and mounted in Litholoops (Molecular Dimensions) before flash-cooling by plunging into liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... All crystallization experiments were set up using an NT8 drop-setting robot (Formulatrix Inc.) and UVXPO MRC (Molecular Dimensions) sitting drop vapor diffusion plates at 18 °C ...
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bioRxiv - Biophysics 2021Quote: ... Crystallization screening was carried out at 20 °C using commercial screening kits (Hampton Research, Molecular Dimensions and Rigaku Reagents), where the ToPif1-DNA complex was mixed at a 1:1 ratio with the reservoir solution ...
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bioRxiv - Biophysics 2019Quote: ... Subsequently the LCP was slowly injected into another Hamilton syringe filled with crystallization buffer consisting of 100 mM Sorensen buffer pH 5.6 (GERBU) and 30% polyethylene glycol 2’000 (Molecular Dimensions). The crystallization was carried out at 21°C and crystals with an approximate average size of 35×35×3 µm3 were obtained within 3 to 6 days ...
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bioRxiv - Biochemistry 2022Quote: Crystallization of DgGH172c sample at 10 mg ml−1 was screened using commercial kits (Molecular Dimensions and Hampton Research) with vapor diffusion sitting drop method ...
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bioRxiv - Microbiology 2023Quote: ... NatR-NatT was crystallized in 0.2 M Sodium sulfate 0.1 M Bis-Tris propane 7.5 20% w/v PEG 3350 from the crystallization screen Pact Premier (Molecular dimensions) at a final protein concentration of 2.5 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... All crystallization experiments were set up using an NT8 drop setting robot (Formulatrix Inc.) and UVXPO MRC (Molecular Dimensions) sitting drop vapor diffusion plates at 18°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The best crystals for the native construct were obtained from Pact Premier™ crystallization screen 1 condition #39 (0.1 M MMT pH 6.0, 25% w/v PEG 1500) (Molecular Dimensions). For the modified construct ...
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bioRxiv - Biochemistry 2023Quote: ... All crystals were cryoprotected in the crystallization solution supplemented with 25% (v/v) glycerol and mounted in Litholoops (Molecular Dimensions) before flash-cooling by plunging into liquid nitrogen ...
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bioRxiv - Microbiology 2019Quote: ... The best native crystals were grown after 4 to 5 days in C10 and G10 conditions from Morpheus crystallization screen (Molecular dimensions; C10 ...
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bioRxiv - Microbiology 2021Quote: ... Crystal screening of Fab-peptide complexes were performed using the vapor-diffusion hanging drop method using the sparse matrix crystallization screens ProPlex (Molecular Dimensions), Index (Hampton Research) ...
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bioRxiv - Biochemistry 2019Quote: ... and overlaid with 1 µl of precipitant solution from crystallization screens made in-house and/or the commercial MemMeso HT-96 screen (Molecular Dimensions).
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bioRxiv - Biochemistry 2021Quote: Pure CRISPR-Lon protein was concentrated to 20 mg/ml and crystallized using a Gryphon pipetting robot (Art Robbins) and commercial crystallization screens (Molecular Dimensions). Hexagonal crystals appeared after one day in condition D7 of the JCSG+ screen ...
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bioRxiv - Biochemistry 2021Quote: ... Crystallization trials of the nanbody-RBD complex were performed using the vapor-diffusion hanging drop method with the sparse matrix crystallization screens ProPlex (Molecular Dimensions), Index (Hampton Research) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and compound 12b for 20 minutes at room temperature and set up in 96-well sitting drop crystallization screens JCSG+ HT96 (Molecular Dimensions) and Morpheus HT96 (Molecular Dimensions) ...
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bioRxiv - Biochemistry 2023Quote: Purified recombinant RrCβAA was concentrated to 15 mg/mL using a 10 kDa MWCO centrifugal concentrator (Vivaspin) and subjected to sitting drop vapor diffusion crystallization screening with commercial screens from Molecular Dimensions and Hampton Research ...
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bioRxiv - Biochemistry 2023Quote: All crystallization experiments were carried out in an anaerobic chamber with a 95%/5% (N2/H2) atmosphere using the sitting drop vapor diffusion method and 96-well two-drop MRC crystallization plates (Molecular Dimensions). The plates were incubated for one week in the chamber before use ...
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bioRxiv - Biophysics 2020Quote: ... For our native construct-1 we were able to obtain multiple hit conditions and among them the best crystals were obtained at Pact Premier™ crystallization screen 1 condition #39 from Molecular Dimensions, UK ...
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bioRxiv - Biochemistry 2020Quote: ... Initial crystallization hits were obtained exclusively using the precipitant pentaerythritol propoxylate (5/4 PO/OH) in a MIDAS screen (Molecular Dimensions # MD1-59). Crystals were optimized by hanging drop vapor diffusion at 16 °C ...
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bioRxiv - Microbiology 2022Quote: ... The complex peak was pooled and concentrated to 11 mg/ml for crystallization assays which were set up with commercial screening kits (JCSG-plus™ & Morpheus® from Molecular Dimensions; Index™ & PEG/Ion Screen™ from Hampton Research ...
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bioRxiv - Biochemistry 2022Quote: ... Crystallization for c-Src KD in complex with ponatinib was achieved by sitting-drop vapour diffusion at 20 °C in MRC-2 crystallization plates (Molecular Dimensions, Newmarket, Suffolk, England) by mixing 0.5 μL of protein-ligand solution at 8 mg/ml with 0.5 μL of reservoir solution ...
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bioRxiv - Biophysics 2023Quote: ... CjLas1-Grc3 complex and CjLas1 truncated protein (HEPN domain) were first obtained using the sitting drop vapor diffusion method using high-throughput crystallization screening kits (Hampton Research, Molecular Dimensions and QIAGEN). Crystals were then grown in a mixed solution containing 1 μl complex solution and 1 μl of reservoir solution using the hanging drop vapor diffusion method at 16°C ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.0 (The Protein Complex Suite F1, Molecular Dimensions, protein: precipitant ratio 2:1). The crystal was harvested and flash-cooled without adding cryo-protectant ...
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bioRxiv - Neuroscience 2020Quote: ... The protein crystallized in Wizard 1 (Molecular Dimensions), condition #1 ...
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bioRxiv - Biochemistry 2020Quote: SeMet labeled protein was expressed using Selenomet media from Molecular Dimensions. The cultures were initiated from a frozen stock into Selenomet media supplemented with 50 µg/ml of L-methionine and 100 µg/ml carbenicillin ...
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bioRxiv - Developmental Biology 2019Quote: ... japonica protein was expressed in SelenoMethionine Medium Complete (Molecular Dimensions, Suffolk, UK), and grown ...
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bioRxiv - Biochemistry 2024Quote: ... Selenomethionine substituted protein was produced using a premade SelenoMet expression medium (Molecular Dimensions). Cells were grown for 16h in 100 ml Minimal Media starter cultures containing 40 μg/ml methionine ...
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bioRxiv - Biophysics 2021Quote: ... the protein solution was homogenized with 1.5-folds volumes molten 9.9 MAG (Molecular Dimensions, USA) using a gas-tight coupled syringe device (Hampton Research ...
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bioRxiv - Molecular Biology 2020Quote: ... Buffer exchanged proteins were screened for conditions that produced crystals using the NR LBD (Molecular Dimensions), Structure (Molecular Dimensions) ...
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bioRxiv - Biophysics 2020Quote: ... Selenomethionine labeled ORF8 double mutant protein was expressed using SelenoMet Medium Base according to manufacturer’s instructions (Molecular dimensions). Purification was carried out in the same manner as wild-type but the TEV-cleavage step was omitted.
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bioRxiv - Biochemistry 2022Quote: ... The protein in buffer C (2.2 mg/ml) was crystallized using Ligand-Friendly Screening HT-96 (Molecular Dimensions) for the initial screening and protein crystals grew in 0.1 M Bis-Tris Propane pH 6.5 ...
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bioRxiv - Biochemistry 2020Quote: Initial protein crystals were grown at 4°C in condition E10 of the MemGold2-ECO Screen (Molecular Dimensions; 0.05 M Na-acetate pH 4.5 ...
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bioRxiv - Microbiology 2022Quote: ... Nucleotide-free Irgb6-T95D crystals diffracting to 2.05 Å resolution were obtained from sitting drops with a 0.5 μl of protein solution and a 0.5 μl of reservoir solution consisting of 0.2M Sodium malonate pH7.0 (Molecular Dimensions), 20% Polyethylene Glycol 3350 (Molecular Dimensions ...
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bioRxiv - Bioengineering 2021Quote: ... Crystallization experiments were set up in sitting drop geometry with 0.5 µl protein sample equilibrating with 0.5 µl reservoir from screen kits (Hampton and Molecular Dimensions) at room temperature ...
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bioRxiv - Microbiology 2021Quote: Nucleotide-free Irgb6 crystals diffracting to 1.9 Å resolution were obtained from sitting drops with a 12 mg/ml protein solution and a reservoir solution consisting of 0.1 M MIB buffer pH 6.0 (Molecular Dimensions), 25% Polyethylene Glycol 1500 (Molecular Dimensions ...
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bioRxiv - Biochemistry 2020Quote: ... This preparation of the protein was mixed 1 μL:1 μL with the Morpheus HT screen reagents (Molecular Dimensions) in a 96-well SwissSci MRC UV-transmissible sitting drop plate ...
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bioRxiv - Plant Biology 2021Quote: ... Drops composed of 0.3 μL of protein solution and 0.3 μL of reservoir solution were set up in MRC 96-well crystallisation plates (Molecular Dimensions), which were dispensed using an Oryx Nano or an Oryx8 robot (Douglas Instruments) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.3 μl of 11 mg/ml protein was combined with 0.3 μl reservoir solution from the commercial Morpheus screen (Molecular Dimensions). Crystals appeared in several conditions ...
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bioRxiv - Biochemistry 2022Quote: ... 200 nl of protein was added to 200 nl of precipitant containing 32% low MW PEG smear solution (Molecular Dimensions) and 100 mM Tris pH 8.5 ...
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bioRxiv - Plant Biology 2023Quote: ... Drops composed of 0.3 μL of protein solution and 0.3 μL of reservoir solution were set up in MRC 96-well crystallisation plates (Molecular Dimensions), which were dispensed using an Oryx Nano or an Oryx8 robot (Douglas Instruments) ...
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bioRxiv - Cell Biology 2019Quote: ... The crystals were obtained using the sitting-drop vapor-diffusion method at 18°C by mixing 300 nl of protein-ligand complex with a reservoir solution (Morpheus screen; Molecular dimensions). The diffraction data set was collected at BESSY-II beamline (Mueller et al. ...
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bioRxiv - Biochemistry 2020Quote: ... were obtained at a protein concentration of 8 mg/ml in the conditions “B11” from the Morpheus II crystallisation screen (Molecular Dimensions): 2 mM divalents mix (0.5 mM MnCl3 ...
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bioRxiv - Biochemistry 2019Quote: ... Droplets of 0.1 µL protein solution at 10 mg mL−1 were mixed with an equal volume of reservoir solution using screens from Molecular Dimensions (PACT ...
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bioRxiv - Microbiology 2022Quote: Initial crystallisation trials were performed in 96-well plates using sitting drops composed of 200 nL protein plus 200 nL reservoir solution Initial crystals were identified in broad matrix screens (PACT PREMIER, Molecular Dimensions) and diffraction-quality crystals of Trag25_CTD grew in 0.1 M SPG ...
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bioRxiv - Microbiology 2023Quote: Crystallization conditions for falcilysin were screened with a protein concentration of 22 mg⋅ml-1 using commercial kits (JCSG-plusTM & Morpheus from Molecular Dimensions; Index & PEG/Ion ScreenTM from Hampton Research ...
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bioRxiv - Biophysics 2021Quote: Initial crystallisation hits for the 31-145 DM CtIP protein were observed with condition F6 of the Morpheus® commercial screen MD-47 (Molecular Dimensions). Crystals were improved by hanging-drop vapor diffusion and micro-seeding ...
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bioRxiv - Biochemistry 2021Quote: ... The CodB protein with 1mM cytosine was mixed with monoolein at 60:40 (w/w) ratio using a coupled syringe device (Molecular Dimensions Ltd UK) and crystallisation trials were set up at 20°C using glass sandwich plates using a Mosquito Robot ...
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bioRxiv - Molecular Biology 2024Quote: ... The crystal for structural determination of HSPB7ACD C131S was discovered and harvested after setting up for 10 days from a well containing 0.2 M magnesium formate dihydrate and 20% w/v polyethylene glycol (PEG) 3350 (JCSG-plus A5, Molecular Dimensions, protein: precipitant ratio 2:1). Crystals were cryoprotected using 10% glycerol in well solution ...