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Citations for Electron Microscopy Sciences :
151 - 200 of 238 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Samples were taken prior to the experiment (‘Pre’) and at each time point by diluting 50 μl of cells 1:1 with 2% glutaraldehyde (EMS, 16220) and incubating at 4°C to allow cells to sediment ...
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bioRxiv - Biochemistry 2021Quote: Samples (4 μL) were applied to glow-discharged continuous carbon film supported copper grids (CF300-CU-50 grids, Electron Microscopy Sciences). After 1 min incubation ...
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bioRxiv - Biochemistry 2022Quote: ... was carried out on the various samples with 200-mesh gold grids coated with extra thick (25-50 nm) formvar-carbon film (EMS, USA) made hydrophilic by glow discharging at 5 mA for 60 s ...
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bioRxiv - Neuroscience 2022Quote: ... at a dose of 50 mg/kg 4 hours prior to intracardiac perfusion with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 15714) in PBS.
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Negative stain TEM was carried out on the various samples with 200-mesh gold grids coated with extra thick (25-50 nm) formvar-carbon film (EMS, USA) made hydrophilic by glow discharging at 5 mA for 60 s ...
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bioRxiv - Cell Biology 2024Quote: ... and a 50% resin solution (24 g Embed 812, 9 g DDSA, 15 g NMA, 1.2 g BDMA; 14121; Electron Microscopy Sciences) in propylene oxide ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences) for 5 min at 25 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed with acetone and infiltrated with increasing concentrations (25, 50, 75 and 100%; 2 hours each) of EPON resin (Electron Microscopy Sciences). 100% EPON solution was exchanged two times in 12-hour steps ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were dehydrated in graded concentrations of ethanol (30%, 50%, 70%, 90%, 95%, and 100%) and embedded in Epon resin (Electron Microscopy Sciences). Ultrathin sections (80 nm ...
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bioRxiv - Cell Biology 2022Quote: ... 3.5 µl of each sample was applied to the plasma-cleaned C-flat grids (Electron Microscopy Sciences, CF-1.2/1.3-4Au-50), pre-blotted and blotted with filter paper (Whatman 1 ...
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Presynaptic mitochondria volume and abundance increase during development of a high-fidelity synapsebioRxiv - Neuroscience 2019Quote: ... Tape containing sections was attached to a 4-inch silicon wafer in rows using double sided carbon tape (EMS, Cat# 77817-50) and sections were post-stained with uranyl acetate and lead citrate ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were removed from the wells and mounted onto microscope slides using Fluorogel II with DAPI (cat. No. 17985-50, Electron Microscopy Sciences). Coverslips were imaged with confocal microscopy and the proliferation index was evaluated by dividing total cell number by EdU-positive cells.
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bioRxiv - Neuroscience 2020Quote: ... the separation of the brain from the VNC was performed using fine-tipped forceps and MicroTools (Cat #: 50-905-3315, Electron Microscopy Sciences). The time from digestion (the part of the protocol most likely to induce cell stress ...
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bioRxiv - Immunology 2020Quote: ... 10 mM EDTA) to a final concentration of ∼50-200 µg/ml and immediately applied to the glow-discharged grid (Electron Microscopy Sciences). After 30 seconds ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was lysed until no visible pieces remained just prior to use in 15 μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) with a pestle (Electron Microscopy Sciences, 64788) and a LabGen 7B homogenizer (ColePalmer ...
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bioRxiv - Microbiology 2022Quote: ... the cells were dehydrated in increasing concentrations of ethanol (50%-100%) at room temperature and embedded in EPON resin (Electron Microscopy Sciences). Ultrathin sections (70-80 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then fixed for 10 minutes at room temperature in PBSM supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-492). Cells were then washed 3x for 5 minutes in PBS and exchanged into a permeabilization buffer consisting of PBSM + 5 mg/mL BSA (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... At −50 °C samples were washed four times in ethanol for 30 minutes each before gradual infiltration with HM20 resin (Electron Microscopy Sciences). Polymerization of the resin was done with a UV light source and was carried out at −50 °C for 48 hours followed by post-polymerization at room temperature for 2 days.
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bioRxiv - Bioengineering 2023Quote: A 80 × 50 × 20 mm (L × W × T) block of PMMA (Acrylic Plastic 24210-08, Electron Microscopy Sciences, Hatfield, PA 19440, USA) was cast and cured in a mold for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... sections were dehydrated in a series of graded (25, 50, 75, 100, 100, 100 v/v%, 10 min each) acetonitrile (Electron Microscopy Sciences) aqueous solutions ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 µL sample was spotted onto a glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences CF300-Cu-50), incubated for two minutes then gently blotted onto filter paper to remove excess solution ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was carefully removed and cells were fixed for 10 minutes at room temperature with 50 µl of 4% paraformaldehyde freshly prepared from a 16% stock (Electron Microscopy Sciences). Cells were again washed with 800 µl PBS with 1% BSA and pelleted ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein was adsorbed on freshly glow-discharged carbon-film grid (CF200-CU, Electron Microscopy Sciences), washed with dilution buffer and stained with 0.75% w/v uranyl formate ...
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bioRxiv - Cell Biology 2021Quote: Ovaries of the female mice that were in the fertility trials were fixed in Modified Davidsons fixative solution (Electron Microscopy Sciences, #6413-50) for 6–12 h and were processed by the Office of Translational Science at Rutgers University for histology services ...
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bioRxiv - Biophysics 2022Quote: ... The grids were then stained with 50 μL aqueous uranyl acetate solution (2% v/v) (Electron Microscopy Sciences, Philadelphia, USA. Product No: 22400) for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... specimens were post-fixed in secondary fixative (50 mM phosphate buffer pH 6.2 containing 1% glutaraldehyde and 1% osmium tetroxide; Electron Microscopy Sciences, Hatfield, USA) for 40 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... without mPrep screens and held in mPrep CPD Holder (Microscopy Innovations, Cat # 2250) that was then placed in a 50 ml PTFE beaker (EMS Diasum, Cat# 60942) containing LN2 frozen substitution fluid (2% osmium tetroxide in acetone with 3% water ...
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bioRxiv - Neuroscience 2022Quote: ... at a dose of 50 mg/kg either 4 hours or 7 days prior to intracardiac perfusion with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 15714) in PBS.
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
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bioRxiv - Neuroscience 2021Quote: ... the beads with enriched proteins were washed three times with 80% ethanol (Electron Microscopy Sciences Cat. #15055) followed by incubation with 100 μL 50mM ammonium bicarbonate (MilliporeSigma Cat ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were deposited on formvar/carbon-coated 200-mesh copper grids (FCF200-Cu, Electron Microscopy Sciences) for 1 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... A 3.5 μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences) that had been glow discharged for 10 seconds using an easiGlow Glow Discharge Cleaning System (PELCO) ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2.2µL drop of protein was deposited on a Quantifoil R1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Neuroscience 2022Quote: ... specimens were dehydrated using serially graded ethanol solutions (30%, 50%, 70%, 90% and 100%) and infiltrated and embedded in Spurs resin (Electron Microscopy Sciences, Hatfield, PA) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... Slides were washed three times in 1x TBS/0.1% Tween-20 for 10 minutes before being incubated with 200 µL 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-487) in a dark humidity chamber at room temperature for 30 minutes ...
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bioRxiv - Immunology 2021Quote: 5 µl of the sample was deposited on a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences, catalog number FCF400-Cu-50), incubated for 1 min and blotted away ...
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bioRxiv - Neuroscience 2020Quote: ... and placed overnight in a 1:50 dilution of donkey anti–rabbit IgG with bound 10-nm colloidal gold (Electron Microscopy Sciences, catalog 25704) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 50 nm ultra-thin sections were obtained with a Leica UC7 microtome and collected on Formvar-coated copper grids (Electron Microscopy Science, Hatfield, PA). Specimens were stained with 1 % uranyl acetate and 2.5 % lead citrate and imaged using a JEOL JEM 1010 transmission electron microscope with an AMT XR-16 mid-mount 16 mega-pixel CCD camera ...
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bioRxiv - Genetics 2020Quote: ... Austria) and sputter coated with palladium/gold with a thickness of 50 nm in an EMS 575X sputter coater (Electron Microscopy Sciences, Hatfield, PA). The samples were imaged using a ZEISS Crossbeam 540 FIB-SEM microscope (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... The fibrils were re-suspended in MiliQ and then 5 µl of the sample was deposited on the plain carbon support film on 200 mesh copper grid (SKU FCF200-Cu-50, Electron Microscopy Sciences, Hatfield, PA). The grid was glow discharged for 0.5–1 min before adding the sample ...
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bioRxiv - Biophysics 2023Quote: ... The fibril samples were deposited on plain carbon support film on 200 mesh copper grids (SKU FCF200-Cu-50, Electron Microscopy Sciences, Hatfield, PA) after glow-discharge for 0.5 −1 min ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.3-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2021Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP expression in OB was detected using a dual fluorescent protein flashlight (Electron microscopy sciences, Hatfield, PA, USA) prior to surgery ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...