Labshake search
Citations for Electron Microscopy Sciences :
201 - 250 of 259 citations for Testosterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... centrifuged at 3,000 x g for 5 min and clarified samples incubated for 2 h on carbon-coated Formvar nickel grids (300 mesh, Electron Microscopy Science, Pennsylvania) coated with anti-GFLV polyclonal antibodies (Bioreba AG ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples were rinsed three times with PBST for 5 min on ice and then embedded and frozen in Tissue Freezing Medium (Electron Microscopy Sciences, PA). Samples were sectioned (8μm ...
-
bioRxiv - Biochemistry 2020Quote: End-point samples from wt α-syn aggregation assays (5 μL) were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 seconds using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
-
bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
-
bioRxiv - Microbiology 2023Quote: ... Ileum pieces were rinsed 3 times for 5 minutes in PB buffer and then postfixed with a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA, US) with 1.5% potassium ferrocyanide (Sigma ...
-
bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... 5 µg of the sample was placed onto a glow discharged 200-mesh formvar/carbon copper grid (Electron Microscopy Sciences, Hatfield Township, PA) for 5 min ...
-
bioRxiv - Neuroscience 2019Quote: ... NaNO2 (# S25560) and NaCl (# 7647-14-5) which is followed by perfusion with 40 mL of cold 4% paraformaldehyde (Electron Microscopy Sciences, #RT-15714) in 1X-phosphate buffered saline (PBS) ...
-
bioRxiv - Immunology 2021Quote: 5 µl of the sample was deposited on a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences, catalog number FCF400-Cu-50), incubated for 1 min and blotted away ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... The sample was then mounted on a SEM sample holder with double-sided carbon tape and sputter-coated with 5 nm of Pt/Pd (EMS 150 T S Metal Sputter Coater, Electron Microscopy Science, Hatfield, PA). Using the Zeiss SmartSEM software ...
-
bioRxiv - Cell Biology 2019Quote: ... 10 μl of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
-
bioRxiv - Cell Biology 2021Quote: ... The sections are again washed thrice in PBS and stained with DAPI for 5 minutes and mounted with Fluoromount-G solution (17984-25, Electron Microscopy Sciences, Pennsylvania, USA). Using a Zeiss Axio Imager Z1 ApoTome Microscope ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cells were stained en bloc with 0.25% uranyl acetate then dehydrated in graded series of ethanol on ice followed by one wash with 100% ethanol and two washes with propylene oxide (5 min each) and embedded with EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Sections were cut at 60 nm on a Leica EM UC6 ultramicrotome ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The vaccine powder was reconstituted and diluted 5 times with water and then transferred to a glow discharge-treated carbon coated copper grid from Electron Microscopy Sciences (Hatfield, PA). The sample was stained with a 2% uranyl acetate solution for 2 min and then air dried ...
-
bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
-
bioRxiv - Biochemistry 2024Quote: Purified receptor was diluted to ∼5 ug mL−1 and applied to freshly glow-discharged EM carbon coated copper grid (Electron Microscopy Sciences, CF400-Cu,) for 30 sec ...
-
bioRxiv - Biophysics 2024Quote: ... cells were imaged under physiological conditions (5% CO2 and 37°C) via a stage top incubator (Tokai Hit) with type 37 immersion oil (Electron Microscopy Sciences, #16914-01). Fixed cells were imaged at room temperature (22°C ...
-
bioRxiv - Plant Biology 2024Quote: ... embedded using a Quetol 651 - NSA Kit (no. 14640, Electron Microscopy Sciences), and polymerized at 60°C ...
-
bioRxiv - Biophysics 2020Quote: ... 5 uL of DNA origami solution (5 nM) was deposited onto fresh glow discharged carbon film with copper grids (CF200H-CU; Electron Microscopy Sciences Inc., Hatfeld, PA), and the sample was then allowed to absorb onto the surface for 30s ...
-
bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... 7 and 8) or DNA stained with Hoechst 33258 and mounted on glass slides using Fluoromount G (Electron Microscopy Sciences; Figs. 5, 6E, 6G, S1). APEX enzymes and YFPSMO were detected by GFP and YFP fluorescence ...
-
bioRxiv - Cell Biology 2022Quote: ... stained en bloc with aqueous 1.0% uranyl acetate for 1 h on ice, (5) progressively dehydrated through an ethanol series (50%, 70%, 90%, and 100%) and 100% propylene oxide (Electron Microscopy Sciences, Hatfield, PA, USA) each for 10 min ...
-
bioRxiv - Bioengineering 2022Quote: ... Then the sample was incubated with cleavage solution (EMS of the MiSeq kit) at 37°C for 3 × 15 min ...
-
bioRxiv - Cell Biology 2022Quote: ... and embedded in epoxy resin (Embed 812 kit for Electron Microscopy, EMS Company) using standard preparative procedure for ultrastructural analyses of tissues and cells ...
-
bioRxiv - Neuroscience 2022Quote: ... and finally embedding using the Technovit 7100 kit (Electron Microscopy Sciences Cat# 14653). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
-
bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
-
bioRxiv - Cancer Biology 2022Quote: ... and embedded in resin mixture of EMbed 812 Kit (Electron Microscopy Sciences cat#: 14120), cured in a 60°C oven ...
-
bioRxiv - Zoology 2022Quote: ... Samples were embedded with Spurr's Low Viscosity Embedding Media Kit (Electron Microscopy Sciences #14300), following the “Firm” formulation provided by the manufacturer ...
-
bioRxiv - Neuroscience 2019Quote: ... the whole brain was impregnated with Lowicryl HM20 Resin Kits (Electron Microscopy Sciences, cat.no. 14340) by sequential immersions in 50 ...
-
bioRxiv - Neuroscience 2020Quote: ... the whole brain was impregnated with Lowicryl HM20 Resin Kits (Electron Microscopy Sciences Cat#14340) by sequential immersions in 50 ...
-
bioRxiv - Neuroscience 2020Quote: ... the whole brain was impregnated with Lowicryl HM20 Resin Kits (Electron Microscopy Sciences, cat.no. 14340) by sequential immersions in 50 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Pre-embedding enhancement was realized with silver enhancement kit (AURION R-Gent SE-EM, EMS) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Pre-embedding enhancement was realized with silver enhancement kit (AURION R-Gent SE-EM, EMS) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Muscles were further incubated in Spurr:acetone prepared using the Spurr Resin kit (Electron Microscopy Science, 14300), at ratios of 1:2 ...
-
bioRxiv - Neuroscience 2023Quote: ... Slices were silver-enhanced with AURION R-GENT SE-EM kit (Electron microscopy sciences, Cat # 500.033) for 25 mins ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were embedded with Spurr’s Low Viscosity Embedding Kit (cat. no. EM0300-1KT, Electron Microscopy Sciences), sectioned (70-90 nm thick) ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were silver enhanced using the Aurion R-Gent SE-EM Kit (EMS, Cat #25520-90). Following additional post-fixation in 1% osmium tetroxide (EMS ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Dehydrated samples were embedded in BEEM capsules using Spur low viscosity embedding kit (Electron Microscopy Sciences). Blocks were polymerized at 70°C for 8 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were embedded in resin using a low viscosity embedding kit (Electron Microscopy sciences Cat #14300). Embedded samples were cut into 600nm thick sections using a Reichert Ultracut-II-Ultramicrotome with a diamond knife ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were silver enhanced using the Aurion R-Gent SE-EM Kit (EMS, Cat #25520-90). Following additional post-fixation in 1% osmium tetroxide (EMS ...
-
bioRxiv - Neuroscience 2024Quote: ... with each incubation lasting 24 hours at room temperature (Spurr Resin kit, Electron Microscopy Science, 14300). Lumbricals were embedded in 100% Spurr and polymerized at 65°C for 48 hours ...
-
Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... followed by dehydrating in ethanol and embedding using the EM bed 812 kit (Electron Microscopy Sciences) according to a standard procedure ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were then gradually infiltrated with a mixture of low viscosity resin (UltraBed Kit, Electron Microscopy Sciences) and ethanol according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... We then used the established concentrations for the Embed 812 resin kit (Electron Microscopy Sciences, Hatfield, USA) to embed the head capsule while maintaining their brain dimensions ...
-
bioRxiv - Microbiology 2023Quote: Protist pellets were prepared using standard methods for the Embed 812 resin kit (Electron Microscopy Sciences, EMS)60 ...
-
bioRxiv - Microbiology 2023Quote: Protist pellets were prepared using standard methods for the Embed 812 resin kit (Electron Microscopy Sciences, EMS)60 ...
-
bioRxiv - Plant Biology 2023Quote: ... dehydrated in ethanol series and embedded in Araldite-Epon resin (Araldite 502/Embed 812 Kit; Electron Microscopy Sciences, Science Services GmbH ...