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Citations for Electron Microscopy Sciences :
51 - 100 of 133 citations for Siglec 15 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Grids were washed twice with ddH2O for 15 s before negative staining with UranyLess (Electron Microscopy Sciences, Hatfield, PA) for 45 s ...
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bioRxiv - Genetics 2019Quote: ... with 15 µl subsequently loaded on a CELLOMETER™ Disposable Cell Counting Chamber (Electron Microscopy Sciences, Hatfield, PA, USA) and analyzed under a Zeiss Axioskop 2 (Zeiss ...
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bioRxiv - Cell Biology 2022Quote: ... as model cargos and placed in the incubator for 15 min or 3 h incubation followed by washing with PBS and fixation with 2.5% glutaraldehyde (EMS) in 0.1 M phosphate or cacodylate buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... or Ang1 at 100 nM of F-domain concentration for 15 min before fixing with 4% PFA (EMS, 15710) for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Ribbons of 15-20 ultrathin (70 nm) serial sections were cut using a Diatome diamond knife (Electron Microscopy Sciences), mounted on Formvar-coated slot grids (Electron Microscopy Sciences) ...
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bioRxiv - Biophysics 2023Quote: ... Grids were picked up from the glass bottom MatTek dish using 5/15 Style Dumont Tweezers (Electron Microscopy Sciences), transferred to their respective tweezers and placed into the EM GP chamber set at 25°C and 99% humidity or the CP3 chamber ...
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bioRxiv - Developmental Biology 2024Quote: Tissues were fixed between 15 minutes to 3 hours at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Post-fixation samples were dehydrated with ethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... we spread 5×104 protoplasts onto a regeneration plate and subjected them to a treatment of either 6 sec of 15 W UV or 12 μg/ml ethyl methanesulfonate (EMS) to cause random mutations in the genomes ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Plant Biology 2021Quote: ... for one hour and then washed three times for 15 min each with the same buffer before secondary fixation in 1% osmium tetroxide (EMS) prepared in Sorensen’s Phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... 4µL of this diluted sample was applied to 15s 15mA glow discharge CD400-Cu negative stain grids (Electron Microscopy Sciences). Grids were stained using 0.8% uranyl formate ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 expression was confirmed by immunofluorescence with paraformaldehyde (4% f.c, 15 min, room temperature, Electron Microscopy Sciences, Cat. #15713-S) fixed cells ...
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bioRxiv - Neuroscience 2023Quote: Mice (female; n=4) at 15 dpl were perfused with PFA prepared in 0.1 M Sorensen’s buffer (Electron Microscopy Sciences). The spinal cords were harvested ...
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bioRxiv - Immunology 2023Quote: A 15 μL sample containing phage of interest was placed to a TEM grid (Formvar Carbon Film 300 Ni, EMS) for three minutes and then incubated with blocking buffer (2% BSA in PBS Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... a lAAV200,000-labeled MOp mouse sample and a cslAAV20,000-labeled basal forebrain mouse sample were embedded with Technovit 9100 Methyl Methacrylate (MMA, Electron Microscopy Sciences, USA) according to previous procedures with minor optimizations66 ...
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bioRxiv - Biophysics 2022Quote: ... then cells were allowed to adhere for 15 min in media prior to chemical fixation in 2% PFA and 0.2% glutaraldehyde (Electron Microscopy Sciences). FActin was stained by permeabilizing cells with 0.1% Triton-X-100 prior to incubation with 3.3 μM phalloidin-AlexaFluor647 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in gels were transferred to nitrocellulose membranes and subsequently immobilized by 0.1% glutaraldehyde (15 min) (Electron Microscopy Sciences; Hatfield, PA). After blocking in Tris-Buffered Saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... 20 mM NaCl and incubated with secondary 15 nm gold-tagged goat-anti-rabbit IgG F(ab’)2 (Electron Microscopy Sciences) diluted 1:10 in 20 mM Tris pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: Neuronal culture in confluent 12-well plates were fixed directly with gentle agitation for 15 minutes at room temperature with a fixative containing 2.5% glutaraldehyde (Electron Microscopy Sciences), 2% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were fixed for 15 min at RT with 4% electron microscopy grade paraformaldehyde (PFA; Electron Microscopy Sciences, cat. no. 15710) diluted in PIPES–EGTA– magnesium (PEM ...
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bioRxiv - Cancer Biology 2022Quote: ... Target retrieval was performed in citrate buffer at pH 6.0 for 15 minutes at 110°C under pressure and sections were transferred to Sequenza slide staining racks (Electron Microscopy Sciences). Sections were treated with Bloxall (Vector labs ...
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bioRxiv - Cell Biology 2020Quote: ... a sucrose gradient was created using 2.3 ml of a 20 % sucrose solution containing 15 mM HEPES [pH 7.4] and 4 % paraformaldehyde aqueous solution (EM Grade) (Electron Microscopy Sciences) and 2 ml of a 10% sucrose solution containing 15 mM HEPES [pH 7.4] ...
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bioRxiv - Immunology 2021Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Immunology 2020Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Cell Biology 2024Quote: ... and a 50% resin solution (24 g Embed 812, 9 g DDSA, 15 g NMA, 1.2 g BDMA; 14121; Electron Microscopy Sciences) in propylene oxide ...
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bioRxiv - Cell Biology 2021Quote: Mouse inner ears were fixed in 4% paraformaldehyde (EMS Diasum) in PBS for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG/IgM-6nm gold secondary antibody (EMS) diluted 1:25 in 1% BSA PBS was added for 1.5 hours followed by another washing step ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed at 10-15 min post-damage for 45 min with 4% paraformaldehyde (PFA; Cat No – RT-15710, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Two additional 15 min washes in 1X PBS were performed before tissue sections were air dried and mounted (Fluoro-Gel, Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2021Quote: ... washed and incubated for 1 h at room temperature with gold conjugated (10-nm) goat anti-rabbit-IgG (1:15, Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2020Quote: ... Sections were cut at 180 nm and mounted on 15 mm glass coverslips coated with a Tissue Capture Pen (EMS, 71314-10). Sections were then embedded in VectaShield (Vector Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: Neurons were rinsed 1X with PBS at room temperature and then fixed for 15 minutes at room temperature with 4% paraformaldehyde (Electron Microscopy Sciences) made up in PBS and supplemented with 4% sucrose ...
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bioRxiv - Molecular Biology 2019Quote: ... that had been previously plasma treated for 30 seconds using a 15 mA current operating under atmospheric gases using a glow discharger (Electron Microscopy Sciences). Excess sample was blotted away for 4 s using Whatman No ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Developmental Biology 2022Quote: ... ultra-thin sections of 100 nm thickness for TEM analysis were then cut using an ultramicrotome and stained with 33% methanolic uranyl acetate for 15 minutes and lead citrate (Electron Microscopy Sciences) for 7 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... for 15 minutes each at room temperature and then immersed in a series of epoxy resin/100% EtOH exchanges (Electron Microscopy Sciences) with increasing resin concentration (25% resin/75% ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... from native aggregation assays sonicated in a water bath for 10-15 s were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 s using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibody incubation was followed by 4 x 15-minute washes in 1X PBS and slide mounting using Fluor-Gel mounting medium (Electron Microscopy Sciences). Tissues were mounted epithelium-side up.
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bioRxiv - Biophysics 2023Quote: ... Kineisn-1 was diluted in the kinesin buffer to 15-20nM and applied on a glow-discharged carbon grid (Carbon Film 400-mesh copper, Electron Microscopy Sciences). After 1 minute incubation ...
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bioRxiv - Cell Biology 2020Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Microbiology 2019Quote: Equal numbers of bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29 ...
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bioRxiv - Neuroscience 2020Quote: ... the appropriate plane of anesthesia was checked by a lack of toe pinch reflex and the animals were transcardially perfused with 15 ml 0.15 M cacodylate buffer (EMS, Hatfield, PA, pH 7.4) followed by 30 ml fixative mixture containing 0.08 M cacodylate (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Neuroscience 2021Quote: ... the appropriate plane of anesthesia was checked by a lack of toe pinch reflex and the animals were transcardially perfused with 15 ml 0.15 M cacodylate buffer (EMS, Hatfield, PA, pH 7.4) followed by 30 ml fixative mixture containing 0.08 M cacodylate (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (46) ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with Aurion blocking solution for one hour and washed 2 × 15 mins with incubation solution (Electron microscopy sciences, Cat # 25558). Then the slices were incubated with goat anti-BirA in incubation solution (1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were suspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% monomeric formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29) ...
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bioRxiv - Cell Biology 2019Quote: ... and 1:10 Goat-Anti-Mouse IgG (H+L) (EMS 25133) antibodies coupled with 6 nm and 15 nm gold particles ...
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bioRxiv - Developmental Biology 2019Quote: ... Ultra-thin sections of 100 nm thickness for TEM analysis were then cut using an ultramicrotome and stained with 33% methanolic uranyl acetate for 15 minutes and lead citrate (Electron Microscopy Sciences, cat # 22410) for 7 minutes ...
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bioRxiv - Biophysics 2019Quote: ... and then fixed for 15 minutes at room temperature (RT) with pre-warmed (at 37 °C) 3% paraformaldehyde (PFA, 15710, Electron Microscopy Sciences, Hatfield, PA) and 0.1% glutaraldehyde (GA ...