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Citations for Electron Microscopy Sciences :
1 - 50 of 75 citations for Recombinant Enhanced Green Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... EGFP expression in OB was detected using a dual fluorescent protein flashlight (Electron microscopy sciences, Hatfield, PA, USA) prior to surgery ...
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bioRxiv - Neuroscience 2023Quote: ... Contrast was enhanced by en bloc staining with 0.5% uranyl acetate (22400, Electron Microscopy Sciences) before dehydration in ethanol and embedding in Embed 812 (14120 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05% malachite green (EMS) in 0.1 M sodium cacodylate buffer of pH 6.8 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were silver-enhanced with AURION R-GENT SE-EM kit (Electron microscopy sciences, Cat # 500.033) for 25 mins ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were silver enhanced using the Aurion R-Gent SE-EM Kit (EMS, Cat #25520-90). Following additional post-fixation in 1% osmium tetroxide (EMS ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were silver enhanced using the Aurion R-Gent SE-EM Kit (EMS, Cat #25520-90). Following additional post-fixation in 1% osmium tetroxide (EMS ...
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bioRxiv - Cancer Biology 2021Quote: ... Contrast was enhanced by staining with 0.5 % uranyl acetate and 1 % lead citrate (both EMS, Munich, Germany). Samples were viewed at an acceleration voltage of 60 kV using a Zeiss Leo 906 (Carl Zeiss ...
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bioRxiv - Neuroscience 2023Quote: ... Contrast was enhanced using 1% uranyl acetate in 70% ETOH within the dehydration series (EMS, Cat 22400). Sections were then treated with propylene oxide ...
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bioRxiv - Neuroscience 2024Quote: ... Contrast was enhanced using 1% uranyl acetate in 70% ETOH within the dehydration series (EMS, Cat 22400). Sections were then treated with propylene oxide ...
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bioRxiv - Cell Biology 2022Quote: ... the glass coverslips were mounted in an antifade fluorescent mounting medium (Electron Microscopy Sciences, 17966). Images were then obtained using a ZEISS LSM 710 or 880 confocal fluorescence microscope (Carl Zeiss ...
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bioRxiv - Neuroscience 2020Quote: ... 20 mM NaCl and incubated with secondary 15 nm gold-tagged goat-anti-rabbit IgG F(ab’)2 (Electron Microscopy Sciences) diluted 1:10 in 20 mM Tris pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and silver enhanced for 90 minutes using an Aurion R-Gent SE-EM Silver Enhancement Kit (Aurion, Electron Microscopy Sciences, Fort Washington, PA, cat #25521), followed by post-fixation in 0.1% osmium tetroxide for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid monolayers with adsorbed GFP-tagged septin hetero-octamers were collected by briefly placing hydrophobic grids (Electron Microscopy Sciences, Hatfield, PA, USA) on the surface of the solutions with the carbon-coated side facing the solution ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were mounted on glass slides using Fluorescent-G mounting medium (Electron Microscopy Sciences, cat#17984-25) and imaged using an SP8 confocal microscope (Leica ...
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bioRxiv - Genomics 2022Quote: ... Mouse embryonic stem cells were plated on Human recombinant laminin 511-coated coverslips (Electron Microscopy Sciences, Cat. 72196-25). Cells were then fixed using 4% formaldehyde (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Biophysics 2020Quote: For TEM imaging 10 μL of recombinant tau fibril samples were applied to copper grid (Electron Microscopy Science, FCF-300-Cu) cleaned with plasma for 20 s ...
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bioRxiv - Neuroscience 2023Quote: ... Labeled sections were mounted onto glass slides and coverslipped with fluorescent mounting medium (Citofluor AF1; Cat#17970-25, Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed for 20 min on ice with 2.5% GA and 0.05% malachite green (EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Bioengineering 2020Quote: ... and Safranin O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA, USA) were used to stain all study samples in accordance with manufacturer protocols ...
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bioRxiv - Biophysics 2021Quote: Neural cell cultures were fixed post-live-cell fluorescent imaging using a solution of 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) + 8% w/v sucrose in 1× PBS for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... 15 nm protein A-coated gold beads (EMS) were adhered to the grids prior to Reynolds’ lead citrate staining ...
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bioRxiv - Neuroscience 2020Quote: ... and 1 μL of virus mixed with Fast Green FCF Solution 2.5% (Electron Microscopy Sciences, Hatfield, PA, USA). The mouse was placed on a heating blanket (37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 2h at room temperature and stored in 1% PFA in PBS at 4°C and finally fixed with 2.5% GA and 0.05% malachite green (EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µL of protein was applied to Quantifoil grids (EMS) that were glow-discharged for 30s at 15 mA ...
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bioRxiv - Neuroscience 2020Quote: ... and filled with 0.5 µL of virus (titer ∼ 1012) dyed with Fast Green 2.5% (Electron Microscopy Sciences, Hatfield, PA, U.S.A.). The mice (∼8 weeks old ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were cleaned by standard acetone precipitation (EM grade acetone; EMS). The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Each protein was deposited on a CF-400-CU grid (Electron Microscopy Sciences) that had been plasma cleaned for 30 seconds in a Solarus 950 plasma cleaner (Gatan ...
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bioRxiv - Synthetic Biology 2020Quote: Purified protein samples applied to CF300-Cu carbon coated grids (Electron Microscopy Sciences) were prepared for negative stain transmission electron microscopy ...
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bioRxiv - Immunology 2023Quote: ... coli BioParticles™ conjugate or DQ™ Green BSA was added for 30min at 4°C and moved to Cytoseal mounting medium (Electron Microscopy Sciences, Hatfield, PA) set at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences) for 5 min at 25 °C ...
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bioRxiv - Pathology 2019Quote: ... The samples were then sectioned into 5 μm-thick slices and stained with hematoxylin and eosin (H&E; Fisherbrand™ 517-28-2, Waltham, MA) or safranin O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA), following manufacturer protocols.
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bioRxiv - Pathology 2021Quote: ... Sections were stained with hematoxylin and eosin (H&E; Fisherbrand™ 517-28-2, Waltham, MA) or safranin-O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA), following manufacturer protocols ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein was adsorbed on freshly glow-discharged carbon-film grid (CF200-CU, Electron Microscopy Sciences), washed with dilution buffer and stained with 0.75% w/v uranyl formate ...
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bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
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bioRxiv - Neuroscience 2021Quote: ... the beads with enriched proteins were washed three times with 80% ethanol (Electron Microscopy Sciences Cat. #15055) followed by incubation with 100 μL 50mM ammonium bicarbonate (MilliporeSigma Cat ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were deposited on formvar/carbon-coated 200-mesh copper grids (FCF200-Cu, Electron Microscopy Sciences) for 1 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... A 3.5 μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences) that had been glow discharged for 10 seconds using an easiGlow Glow Discharge Cleaning System (PELCO) ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2.2µL drop of protein was deposited on a Quantifoil R1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.3-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2021Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of protein sample was applied to freshly-ionized carbon-coated copper mesh grids (Electron Microscopy Science). After 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of protein was loaded onto a glow-discharged CF200-CU carbon grid (Electron Microscopy Sciences, U.K.). After 5 min of incubation ...
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bioRxiv - Immunology 2023Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 s using a PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Biophysics 2023Quote: A volume of 3.5 μl of purified channel protein was applied onto glow-discharged copper holey carbon grids R1.2/1.3 (Q350CR1.3, Electron Microscopy Sciences), which was cleaned for 1 minute in an H2/O2 plasma using a Gatan Solarus 950 (Gatan) ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...