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Citations for Electron Microscopy Sciences :
101 - 150 of 167 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... plasmids encoding the DENV protein variants were transfected individually into human HEK-293T cells and allowed to express for 22 h before fixing cells in 4% paraformaldehyde (Electron Microscopy Sciences), and permeabilizing with 0.1% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... They were then kept at room temperature for ∼2 h before being applied to carbon-coated 200-hex copper grids (EMS, Hatfield PA). Freshly ionized grids were floated on a 10 ul drop of sample for 1 minute ...
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bioRxiv - Biophysics 2022Quote: ... Samples of cores undergoing ERT for 4 h at 37 °C were mixed with equal volumes of 10-nm BSA Gold Tracer (Electron Microscopy Sciences), and 3.5 uL aliquots were applied onto glow-discharged Quantifoil grids (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2019Quote: ... Cat# H-12-1.5-PLL) and fixed for 23 minutes in fresh 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, Cat. 15710) in PBST ...
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bioRxiv - Neuroscience 2019Quote: ... Cat# H-12-1.5-PLL) and fixed for 23 minutes in fresh 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, Cat. 15710) in PBST ...
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bioRxiv - Microbiology 2021Quote: Cultures were centrifuged and the cells fixed for at least 4 h at room temperature in 10 volumes of 4% paraformaldehyde (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Genetics 2022Quote: ... and PR110 (h+ leu1-32 ura4-D18) were used for three successive rounds of mutagenesis with ethylmethane sulfonate mutagenesis (EMS from Sigma) based on [35] ...
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bioRxiv - Genetics 2023Quote: Testis and epididymides samples from 8-16-week-old mice were fixed for 24 h in PBS/4% PFA (Electron Microscopy Sciences), dehydrated in a graded ethanol series ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent secondary antibodies were incubated in 1% BSA-PBS solution for 1 h at room temperature before being mounted in Fluoromount G (Electron Microscopy Sciences). Acquisitions were performed on an LSM 800 confocal laser-scanning microscope (Zeiss) ...
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bioRxiv - Biochemistry 2020Quote: 5 μL of purified sample was applied to glow-discharged (10s) Quantifoil R 1.2/1.3 grids (Au 400; Electron Microscopy Sciences) and plunge-frozen in liquid nitrogen-cooled liquid ethane ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Immunology 2021Quote: ... A 4-μl drop of the diluted sample was applied to a freshly glow-discharged carbon-coated copper grid (400-mesh, EMS) for approximately one minute ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Bioengineering 2023Quote: 5 μL of D-GQDs/exo loaded on the Poly-L-Lysine coated slides (63410; Electron Microscopy Sciences, PA, USA) and was fully covered with 18 mm × 18 mm square cover glasses (2845-18 ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were put in propylene oxide for 1 h and infiltrated overnight in a 11 mixture of propylene oxide and Spurr’s low viscosity resin (Electron Microscopy sciences, Hatfield, PA). The cells were embedded in Spurr’s resin and polymerized at 60°C for 48 h ...
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bioRxiv - Biochemistry 2019Quote: ... centrifuged at 3,000 x g for 5 min and clarified samples incubated for 2 h on carbon-coated Formvar nickel grids (300 mesh, Electron Microscopy Science, Pennsylvania) coated with anti-GFLV polyclonal antibodies (Bioreba AG ...
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bioRxiv - Plant Biology 2022Quote: ... from −30 °C to 0° in 6 h) This was followed by washing in acetone and then infiltration in Spurr resin (EMS, Hatfield, PA, US) at graded concentration and temperature (30% for 10 h from 0 °C to 20°C ...
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bioRxiv - Cell Biology 2020Quote: ∼5-6μl of the resuspended 100,000 ×g pellet fraction was spread onto glow discharged Formvar-coated copper mesh grids (Electron Microscopy Sciences) and stained with 2% Uranyl acetate for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... purified EVs were resuspended in 30 μL D-MEM (0% FCS) and mixed with a low volume of Glutaraldehyde 25% (final concentration = 2.5%) (16220, Electron Microscopy Sciences). After 10 min of fixation at RT ...
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bioRxiv - Cell Biology 2022Quote: ... For immunofluorescence analysis cells were seeded (1.5 x 105 HeLa cells) directly on poly-L-lysine coated coverslips (Electron Microscopy Sciences), which were placed in a 12-well plate ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Cell Biology 2024Quote: A drop (1-2 μl) of undiluted EVs (PEG/SEC-enriched EVs from 100 uninjured egfp(RNAi) animals) was placed on a formvar (EMS 15810)-coated hexagonal grid (EMS G300-Cu ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed thrice with PBS and smear dried on a Poly-L-lysine coated slide (Electron Microscopy Science: 63410), mounted with Prolong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... were adsorbed for 30 s (for high salt conditions) to 1 h in a humid chamber (for low salt conditions) on a glow-discharged carbon-coated grid (Electron Microscopy Sciences, CF300-CU). For low salt conditions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and held at that temperature for an additional 12 h before warming to room temperature and infiltrating into Epon-Araldite resin (Electron Microscopy Sciences, Port Washington PA). Samples were flat-embedded between two Teflon-coated glass microscope slides and the resin polymerized at 60 °C for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... stained en bloc with aqueous 1.0% uranyl acetate for 1 h on ice, (5) progressively dehydrated through an ethanol series (50%, 70%, 90%, and 100%) and 100% propylene oxide (Electron Microscopy Sciences, Hatfield, PA, USA) each for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 2 % PFA and allowed to attach on poly-L-lysine-coated 12 mm glass coverslips (Electron Microscopy Sciences, Hatfield, Pennsylvania) for 30 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... sectioned (thickness of 7-10 µm) and put at the center of poly-L-lysine covered glass slides (63478-AS, Electron Microscopy Sciences).
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bioRxiv - Biochemistry 2020Quote: ... An aliquot (approximately 4 μL) of diluted sample was placed on an ultra-thin carbon-coated 200-mesh copper grid (CF200-Cu-UL, Electron Microscopy Sciences, Hatfield ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide fibrils were obtained as described a ove and 5 μl of the sample was spotted on non-glow discharged carbon film 300 mesh copper grids (CF300-CU from Electron Microscopy Sciences) and incubated for 1 min ...
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bioRxiv - Biochemistry 2019Quote: ... were prepared by applying 3 – 5 μL of in vitro assembled PLP (with or without PC portal rings) onto 300-mesh carbon coated copper grids (Electron Microscopy Sciences). Samples were absorbed for 1 minute and washed with 2-3 drops of water ...
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bioRxiv - Biophysics 2020Quote: 4 μl of 22 μM tau aggregation samples were adsorbed onto glow-discharged 200 mesh formvar carbon coated copper grids (Electron Microscopy Sciences) and stained with aqueous uranyLess (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: Purified eCIS samples were briefly centrifuged to remove impurities and 5 μL of supernatant was applied onto freshly glow discharged carbon-coated copper grids (CF400-CU, 6 nm, 400 mesh, Electron Microscopy Sciences). Samples were allowed to adsorb for 2 minutes before being blotted off by filter paper and grids were washed twice with filtered distilled water ...
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bioRxiv - Bioengineering 2023Quote: A 80 × 50 × 20 mm (L × W × T) block of PMMA (Acrylic Plastic 24210-08, Electron Microscopy Sciences, Hatfield, PA 19440, USA) was cast and cured in a mold for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...
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Design and Evaluation of Nanoscale Materials with Programmed Responsivity towards Epigenetic EnzymesbioRxiv - Bioengineering 2024Quote: A drop of nanoparticle sample obtained from the self-assembly of PEG-block-poly (acetylated L-lysine) co-polypeptide was placed on a 300-mesh Formvar carbon-coated copper TEM grid (Electron Microscopy Sciences) for 1 min and wicked off ...
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bioRxiv - Biophysics 2024Quote: 3 µL diluted MgtA (0.01 mg/mL) was deposited on a carbon-coated 400 mesh copper grid (CF400-CU, EMS, Hatfield, PA, USA) following glow discharging (PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Developmental Biology 2022Quote: ... with commercial antigen unmasking solution (VECTOR, Catalog #: H-3300) and underwent immunostaining procedure using the Sequenza™ Immunostaining Center System (Electron Microscopy Sciences, Catalog #: 71407-01)(Zhao et al. ...
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bioRxiv - Cell Biology 2021Quote: Cells were cultured on cell-tight C-2 cell disks (Sumitomo Bakelite, Tokyo, Japan; #MS-0113K) and fixed for 2 h on ice in 2.5% glutaraldehyde (Electron Microscopy Science, Hatfield, PA; #111-30-8) in 0.1 M phosphate buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μL of sample solution was applied onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS, Hatfield, PA, USA). The sample solution was left for 30 s before blotted with a piece of filter paper ...
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bioRxiv - Genetics 2020Quote: ... Tissue was transferred to a drop of Schneider’s on a silanized coverslip and gently squashed onto a Poly-L-Lysine coated slide (Electron Microscopy Sciences, Hatfield, PA). Coverslips were immediately removed after freezing in liquid nitrogen ...
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bioRxiv - Pathology 2019Quote: ... The samples were then sectioned into 5 μm-thick slices and stained with hematoxylin and eosin (H&E; Fisherbrand™ 517-28-2, Waltham, MA) or safranin O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA), following manufacturer protocols.
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bioRxiv - Pathology 2021Quote: ... Sections were stained with hematoxylin and eosin (H&E; Fisherbrand™ 517-28-2, Waltham, MA) or safranin-O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA), following manufacturer protocols ...
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bioRxiv - Bioengineering 2022Quote: ... 4μL of ~1 nM purified DNA nanostructure was deposited onto a copper TEM grid coated with carbon and formvar (Electron Microscopy Sciences, Hartfield, PA) and incubated for 4 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... A 5 μL sample of the peak fraction was then added to glow discharged copper CF400-CU EM grids (cat. # 71150, Electron Microscopy Sciences, Hatfield, PA) for 1 min ...