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Citations for Electron Microscopy Sciences :
1 - 50 of 2645 citations for R 4 5 Isopropylidene 2 pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Quantifoil R 2/2 (first dataset) and quantifoil R 0.6/1 (second dataset, Electron Microscopy Sciences) grids were washed with ethyl acetate and glow discharged using a Pelco easiGlow (Pelco ...
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bioRxiv - Biochemistry 2021Quote: ... in the presence of 1 mM ATPγS for 5 min prior to Vitrobot freezing on 300 mesh R 2/2 Quantifoil copper grids (Electron Microscopy Sciences). All three datasets were collected on a FEI Titan Krios TEM with a GATAN post column energy filter (20 eV zero-loss filtering ...
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bioRxiv - Biochemistry 2024Quote: Quanifoil R 2/2 200 mesh copper grids (EMS #Q2100-CR2) were glow discharged at 25mA for 25s using a Pelco easiGlow system (Ted Pella) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Quantifoil R 2/2 copper 200 mesh grids (Electron Microscopy Sciences #Q2100CR2) were glow-discharged for 45 seconds at 15 mA using a PELCO easiGLOW Glow Discharge System to apply a negative charge to their surface ...
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bioRxiv - Cell Biology 2022Quote: ... R 2/2 carbon film gold support Quantifoil grids (Q2100AR2; Electron Microscopy Sciences) were loaded into the vacuum chamber and carbon was deposited under high vacuum with stage rotation ...
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bioRxiv - Immunology 2022Quote: ... Quantifoil R 2/1 holey carbon copper grid (EMS) were used for sample vitrification ...
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bioRxiv - Plant Biology 2021Quote: ... The roots of 7 to 10 day old seedlings were collected and fixed for 30 min with 2-(N-morpholino) ethane sulfonic acid (MES) buffer solution pH 5.7 containing 4% paraformaldehyde (32% methanol-free solution; Electron microscopy Sciences). pH was adjusted to 5.7 with KOH solution.
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Molecular Biology 2022Quote: Quantifoil grids (R 2/1 Cu 300 mesh, Electron Microscopy Sciences) were glow-discharged for 40 s prior to the application of 3.5 µl of the sample (∼6 mg/ml protein with 8 mM CHAPSO) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5 mL of 4% Paraformaldehyde (PFA) (EMS) diluted in RNase free PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, R 15710, Hatfield, PA) with 4% sucrose (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cells were loaded onto Cu Finder R 2/2 EM grids (Electron Microscopy Sciences, Hatfield, USA), coated with 1 mg/ml poly-L-lysine ...
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bioRxiv - Pathology 2024Quote: ... 4% PFA was supplemented with 2% glutaraldehyde (EMS). For biochemical analyses such as immunoblotting ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by a 5-minute wash with 1x R-Buffer A (Electron Microscopy Sciences, USA). Antigen retrieval was performed with 1x R-Buffer A at 95°C for 11 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... fixed with 4% PFA for 1minute and stained with 2% acetate uranyl (22400-2, EMS) for 1 minute ...
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bioRxiv - Cell Biology 2024Quote: ... fixed 1 min with 4% paraformaldehyde and stained with 2% uranyl acetate (EMS, 22400-2) for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells were plated at 30,000 cells/cm2 on 200 mesh gold R 2/2 carbon Quantifoil EM grids (Electron Microscopy Sciences).
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bioRxiv - Biophysics 2024Quote: ... 3 μL of chromosome solution was applied onto Quantifoil 200 mesh gold or copper R 2/2 extra thick carbon London Finder EM grids (Electron Microscopy Sciences). Excess liquid was blotted from the back (non-sample ...
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bioRxiv - Biophysics 2021Quote: ... fixed in either 2% glutaraldehyde or 4% paraformaldehyde (Electron Microscopy Sciences) in CB for 20 min room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 in needle containing 2 mL 4% PFA (Electron Microscopy Sciences) diluted in stabilization buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were fixed in 2% or 4% paraformaldehyde (Electron Microscopy Sciences) for 10 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... 2% and 3% OsO4 (prepared from 2 mL 4% osmium tetroxide solution, Electron Microscopy Sciences, Hatfield, PA)
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bioRxiv - Molecular Biology 2023Quote: Carbon-coated holey grids Quantifoil R 0.6/1 Cu 300 and CF-2/1-3C (EMS) were used for preparing cryo-EM samples for apo GSEC1B and GSEC1B-Aβ46 complex ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with freshly prepared and filtered 1% tannic acid (TA, CAS#1401-55-4, EMS) in water using a Pelco BioWave microwave for seven 1-minute cycles alternating between 150 W and 0 W power ...
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bioRxiv - Molecular Biology 2024Quote: Enucleated eyes were fixed for 2 hours at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science, Hatfield, PA) prepared in phosphate-buffered saline (1× PBS ...
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bioRxiv - Developmental Biology 2024Quote: Larvae were fixed for 2 hours at room temperature or overnight at 4° C in 4% paraformaldehyde (EMS catalog # 15710) and 4% sucrose (MP Biomedicals catalog # 802536 ...
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bioRxiv - Biophysics 2024Quote: 3 μL of purified MscS between 0.1 and 0.7 mg/mL was applied to a glow discharged Quantifoil R 1.2/1.3 + 2 nm C 400 Cu mesh grid (EMS), a holey grid with a thin 2 nm continuous carbon film ...
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bioRxiv - Biochemistry 2024Quote: Two μl of each FAN1-PCNA-DNA complex sample was applied to a 300-mesh copper Quantifoil R 1.2/1.3 + 2 nm holey carbon grid (EMS), which was previously glow-discharged for 90 seconds at 15 mA current using an easiGlow (PELCO) ...
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Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin WoundsbioRxiv - Bioengineering 2023Quote: ... they were stained in Phosphomolybdic Acid-Phosphotungstic Acid (EMS) for 15 minutes and then in Aniline Blue (EMS ...
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bioRxiv - Cell Biology 2022Quote: SARS-CoV-2-infected monolayers were fixed with 4% paraformaldehyde (Electron Microscopy Sciences) for 3 hrs at RT ...
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bioRxiv - Developmental Biology 2023Quote: 2 and 8 dpf larvae were fixed in 4% paraformaldehyde (Electron Microscopy Science) 1X PBS (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... the EV preparations were fixed overnight at 4°C in 2% PFA (EMS) and 5μL of sample applied to formvar coated gold grids (EMS ...
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bioRxiv - Neuroscience 2020Quote: ... Shi/Shi:Rag2-/- mice grafted with control and RRMS hiOLs (n=4 per group) were perfused with 1% PBS followed by a mixture of 4% paraformaldehyde/5% glutaraldehyde (Electron Microscopy Science) in 1% PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... the frozen sections were postfixed 5 min with 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in PBS at RT ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Neuroscience 2021Quote: ... first with PBS (< 2 min), then with fixative (4% paraformaldehyde w/v, Sigma; 0.1-2 glutaraldehyde, v/v, Electron Microscopy Sciences (EMS) or TAAB Laboratories Equipment Ltd. ...
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bioRxiv - Neuroscience 2020Quote: ... cleared in xylene (2×5 minutes) and embedded in Entellan (EMS, Hatfield, PA).
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bioRxiv - Developmental Biology 2023Quote: ... Grids were stained with 5 μl of 2% uranyl acetate (Electron Microscopy Sciences) for 45 seconds ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM MgCl pH 6.8 and 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), for 5-10 minutes at 37°C and washed three times in PBS containing 0.1 M glycine (PBS/Gly) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed after 5 days of differentiation with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS and immunofluorescence staining was performed with FoxA2 (1:50 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... 10% phosphotungstic acid/10% phosphomolybdic acid (19500/19400; Electron Microscopy Sciences) and then Aniline Blue (B8563 ...
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bioRxiv - Biophysics 2024Quote: ... 2.5 µl of sample was applied to 300 mesh Quantifoil R 2/1 copper grids (Electron Microscopy Sciences, Hatfield PA) that had been previously glow discharged (15 mA ...
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bioRxiv - Biochemistry 2024Quote: Prom1 and Ttyh1 EVs were vitrified on 300-mesh gold Quantifoil R 1.2/1.3 + 2 nm Carbon grids (Electron Microscopy Sciences). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... a 4 μL aliquot was applied to a Quantifoil 2/2 carbon-coated 200 mesh copper grid (Electron Microscopy Sciences, Hatfield, PA) that was glow-discharged for 30 sec at 20 mA in a Pelco Easi-Glow discharge device (Ted Pella ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu, Electron Microscopy Sciences) followed by plunging into liquid ethane using an FEI Vitrobot Mark IV set to a blot time of 22 and a blot force of 22 for QYNNENNFV and 24 for GSTVYAPFT ...