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Citations for Electron Microscopy Sciences :
1 - 50 of 70 citations for Mouse anti Enterovirus 71 VP1 3657 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Sorted germ cells and SPZEJ were processed as described previously (51, 71) and attached to UltraStick/UltraFrost Adhesion slides (Electron Microscopy Sciences). Samples were fixed in 4% PFA in PBS for 15 min before antigen retrieval in three consecutive 5-min incubations with boiling citrate (10 mM at pH 6) ...
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bioRxiv - Cell Biology 2023Quote: ... The hindlimbs were then submerged in a 100 mL glass beaker filled with 20 mL of Karnovsky fixative [71] ((2.5% EM-grade glutaraldehyde (Electron Microscopy Sciences #16019) plus 2.0% EM-grade paraformaldehyde (Electron Microscopy Sciences #15714-S ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG/IgM-6nm gold secondary antibody (EMS) diluted 1:25 in 1% BSA PBS was added for 1.5 hours followed by another washing step ...
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bioRxiv - Cell Biology 2019Quote: ... and 1:10 Goat-Anti-Mouse IgG (H+L) (EMS 25133) antibodies coupled with 6 nm and 15 nm gold particles ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used were 6 nm anti-mouse immunogold particles (Electron Microscopy Sciences). Negative staining was carried out as previously described [17] ...
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bioRxiv - Microbiology 2023Quote: ... followed by 12 nm gold conjugated goat anti-mouse IgG (Electron Microscopy Sciences) (1:20 dilution) ...
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bioRxiv - Neuroscience 2023Quote: ... including Goat-anti-Human IgG (H&L) and Goat-anti-Mouse IgG (H&L) (Electron Microscopy Sciences, Hatfield, PA) (1:20 dilution in 1% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
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bioRxiv - Cell Biology 2023Quote: ... labeled with a secondary anti-rabbit or anti-mouse gold (depending on the primary antibody used) at RT for 1 hour (1:200 goat-anti-rabbit or goat-anti-mouse, Electron Microscopy Sciences), washed in 1% PBS with 3 sequential 50 µL drops ...
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bioRxiv - Neuroscience 2024Quote: ... A secondary antibody (goat anti-mouse IgG conjugated to 15 nm colloidal gold; Electron Microscopy Sciences) diluted 1:20 in 0.1% BSA in PBS was added for 1.5 h ...
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bioRxiv - Cell Biology 2021Quote: ... donkey anti-mouse IgG (H&L) conjugated with 15-nm gold particles (Electron Microscopy Science Aurion #25817, PA, USA).
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bioRxiv - Bioengineering 2023Quote: ... the grids were incubated in a drop of Goat-anti-Mouse IgG coupled with 10 nm gold nanoparticles (Electron Microscopy Sciences, 25512) diluted at 1:100 in PBS containing 0.1% BSA for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... a lAAV200,000-labeled MOp mouse sample and a cslAAV20,000-labeled basal forebrain mouse sample were embedded with Technovit 9100 Methyl Methacrylate (MMA, Electron Microscopy Sciences, USA) according to previous procedures with minor optimizations66 ...
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bioRxiv - Cell Biology 2021Quote: Mouse inner ears were fixed in 4% paraformaldehyde (EMS Diasum) in PBS for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: Mouse testes were fixed in 2.5% glutaraldehyde solution (EMS, Hatfield, PA, USA) in phosphate buffer (PB 0.1 M [pH 7.4] ...
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bioRxiv - Neuroscience 2023Quote: ... For primary mouse cortical neurons coverglass #1.5 (Electron Microscopy Sciences, Hatfield, PA) were coated for at least 16 hours at 37 °C ...
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bioRxiv - Immunology 2023Quote: Mouse tissue was dissected and fixed in 4% PFA (Electron Microscopy Sciences) before delivery to the Dana Farber/Harvard Cancer Center (DF/HCC ...
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bioRxiv - Cell Biology 2022Quote: ... mouse eyeballs were fixed o/n in 4% (w/v) PFA (171010; EMS) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Primary rat and mouse cardiomyocytes were fixed in 4% PFA (Electron Microscopy Sciences) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: Mouse brain coronal sections were mounted on plus slides (Catalogue number: EMS 71869-11) and dried for 2-3 hours on a slide drier ...
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bioRxiv - Bioengineering 2023Quote: The jejunum of a sacrificed mouse was fixed in 4% paraformaldehyde (Electron Microscopy Sciences), washed in PBS ...
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bioRxiv - Neuroscience 2024Quote: Mouse cochleae were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer (EMS, #15960) supplemented with 2 mM CaCl2 for 1.5-2 hours at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Mouse lungs were inflated to 15 cm of H2O and fixed with 4% paraformaldehyde (EMS) for 24 h ...
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bioRxiv - Physiology 2021Quote: ... the mouse liver pieces were embedded in Embed 812 epoxy resin hard (Electron Microscopy Science) for 12 h and polymerized at 60°C during 48 h ...
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bioRxiv - Developmental Biology 2021Quote: Mouse brain sections/organoid sections were mounted on plus slides (Catalogue number: EMS 71869-11) and dried for 2-3 hours ...
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bioRxiv - Cell Biology 2023Quote: ... adult lungs of Tnrc6Agt/+ mouse were perfused intratracheally with 0.25% glutaraldehyde (Electron Microscopy Science, #16220) at a constant pressure of 30cm H2O for 30min ...
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bioRxiv - Immunology 2023Quote: Harvested mouse organs (kidney, lung) were preserved in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours before being processed and embedded in paraffin-blocks ...
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bioRxiv - Neuroscience 2019Quote: ... the mouse’s head with marked receptive fields and a micro-ruler (Electron Microscopy Sciences, #62096-08) were photographed (13 megapixel camera ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse lungs were inflated to 15 cm H2O and fixed with 4% paraformaldehyde (EMS, Hatfield, PA, USA) for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: Human or mouse FFPE tumor tissue sections were deparaffinized in xylene alternative (Histo-Clear, EMS; 3×5 min) and rehydrated in EtOH/H2O gradient series (100% ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse hindlimbs were fixed in 1 x PBS containing 1.5% glutaraldehyde/1.5% formaldehyde (Electron Microscopy Sciences, Cat#: 15950), 0.05% tannic acid at 4 °C for overnight with gentle agitation ...
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bioRxiv - Genomics 2022Quote: ... Mouse embryonic stem cells were plated on Human recombinant laminin 511-coated coverslips (Electron Microscopy Sciences, Cat. 72196-25). Cells were then fixed using 4% formaldehyde (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The grids were washed twice with blocking buffer before labeling with secondary antibodies (goat anti-rat or goat anti-rabbit, 10nm, Electron Microscopy Sciences), rinsed three times in 1X PBS ...
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bioRxiv - Systems Biology 2023Quote: Mouse hearts were briefly perfused with 10ml cold PBS and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Cat# 15710) (overnight ...
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bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were mounted with anti-fade mounting medium Fluoro-Gel (EMS). All images were taken with a Zeiss LSM710 NLO confocal microscope ...
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bioRxiv - Neuroscience 2021Quote: ... and coverslipped with Fluoromount® anti-fade medium (Electron Microscopy Science). Image acquisition was performed using a Zeiss LSM800 confocal microscope supported by ZEN Imaging Software (Carl Zeiss Canada).
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bioRxiv - Immunology 2023Quote: ... sections prepared from mouse lungs frozen in OCT were stained with Sirius Red (0.1% Sirius Red in saturated picric acid, Electron Microscopy Sciences, 26357-02) for 60 min followed by a 2-min 0.01 N HCl wash ...
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bioRxiv - Neuroscience 2021Quote: ... and coverslipped with Citifluor anti-fade mounting medium (#17970, Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2019Quote: ... incubated for 45 minutes with goat anti-rabbit 15nm gold antibody (EMS) diluted 1:20 in filtered blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... 1:100 goat anti-rabbit IgG 0.8nm immunogold (Aurion, Electron Microscopy Sciences), for 16 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Postnatal mouse were transcardially perfused with 15ml (P14) or 25ml (>P21) of 2% PFA freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Cell Biology 2021Quote: ... Goat-anti-Rabbit IgG 10 nm gold (Electron Microscopy Sciences, Hatfield PA, USA) was then used at a 1:20 dilution for an hour ...
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bioRxiv - Neuroscience 2021Quote: ... or rat-anti-CD63 (MBL, D263-3)) in 0.1% BSAc (Electron Microscopy Sciences) for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then coverslipped using Citifluor anti-fade mounting medium (#17970, Electron Microscopy Sciences).
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bioRxiv - Microbiology 2021Quote: ... and 10nm gold bead conjugated goat anti-rat (Electron Microscopy Sciences, 1:100 dilution), or with goat anti-rat only as a negative control ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with anti-rabbit secondary antibody conjugated to gold particles (Electron Microscopy Sciences) in the blocking solution for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... and then mounted onto Superfrost® micro-slides with Fluoromount® anti-fade medium (Electron Microscopy Science). Epifluorescence images were taken using Axio Observer microscope equipped with Apotome.2 module ...
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bioRxiv - Neuroscience 2023Quote: ... sliced were incubated with donkey-anti-goat UltraSmall gold (1:50, Electron microscopy sciences, Cat #25800, RRID:AB_2631210) at 4°C for 24 hrs with mild agitation ...
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bioRxiv - Neuroscience 2023Quote: ... The mounted sections were then coverslipped with Citifluor anti-fade mounting medium (catalog #17970, Electron Microscopy Sciences) and stored in a microscope slide storage book at 4°C until imaging.