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Citations for Electron Microscopy Sciences :
1 - 50 of 823 citations for Mouse Anti Toxoplasma gondii SAG 1 protein 6207 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Toxoplasma-infected cells were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in 0.25 M HEPES (ph7.4 ...
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bioRxiv - Microbiology 2022Quote: Monolayers of HFF infected with Toxoplasma from 6 or 24 h were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, PA) in 0.25 M HEPES (ph7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... and 1:10 Goat-Anti-Mouse IgG (H+L) (EMS 25133) antibodies coupled with 6 nm and 15 nm gold particles ...
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bioRxiv - Cell Biology 2023Quote: ... labeled with a secondary anti-rabbit or anti-mouse gold (depending on the primary antibody used) at RT for 1 hour (1:200 goat-anti-rabbit or goat-anti-mouse, Electron Microscopy Sciences), washed in 1% PBS with 3 sequential 50 µL drops ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG/IgM-6nm gold secondary antibody (EMS) diluted 1:25 in 1% BSA PBS was added for 1.5 hours followed by another washing step ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used were 6 nm anti-mouse immunogold particles (Electron Microscopy Sciences). Negative staining was carried out as previously described [17] ...
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bioRxiv - Microbiology 2023Quote: ... followed by 12 nm gold conjugated goat anti-mouse IgG (Electron Microscopy Sciences) (1:20 dilution) ...
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bioRxiv - Neuroscience 2023Quote: ... including Goat-anti-Human IgG (H&L) and Goat-anti-Mouse IgG (H&L) (Electron Microscopy Sciences, Hatfield, PA) (1:20 dilution in 1% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
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bioRxiv - Neuroscience 2024Quote: ... A secondary antibody (goat anti-mouse IgG conjugated to 15 nm colloidal gold; Electron Microscopy Sciences) diluted 1:20 in 0.1% BSA in PBS was added for 1.5 h ...
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bioRxiv - Cell Biology 2021Quote: ... donkey anti-mouse IgG (H&L) conjugated with 15-nm gold particles (Electron Microscopy Science Aurion #25817, PA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... mouse hindlimbs were fixed in 1 x PBS containing 1.5% glutaraldehyde/1.5% formaldehyde (Electron Microscopy Sciences, Cat#: 15950), 0.05% tannic acid at 4 °C for overnight with gentle agitation ...
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bioRxiv - Cell Biology 2021Quote: ... 1:100 goat anti-rabbit IgG 0.8nm immunogold (Aurion, Electron Microscopy Sciences), for 16 hours at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... the grids were incubated in a drop of Goat-anti-Mouse IgG coupled with 10 nm gold nanoparticles (Electron Microscopy Sciences, 25512) diluted at 1:100 in PBS containing 0.1% BSA for 1 h ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Microbiology 2021Quote: ... and 10nm gold bead conjugated goat anti-rat (Electron Microscopy Sciences, 1:100 dilution), or with goat anti-rat only as a negative control ...
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bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... washed and incubated for 1 h at room temperature with gold conjugated (10-nm) goat anti-rabbit-IgG (1:15, Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibodies were removed using washing buffer (0.1% BSA in 0.1 M PB) and grids were incubated with 6 nm gold-conjugated donkey-anti-rabbit IgG (1:25, #25104, Electron Microscopy Sciences) for 20 min.
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bioRxiv - Cell Biology 2019Quote: ... 15 nm protein A-coated gold beads (EMS) were adhered to the grids prior to Reynolds’ lead citrate staining ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µL of protein was applied to Quantifoil grids (EMS) that were glow-discharged for 30s at 15 mA ...
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bioRxiv - Neuroscience 2023Quote: ... sliced were incubated with donkey-anti-goat UltraSmall gold (1:50, Electron microscopy sciences, Cat #25800, RRID:AB_2631210) at 4°C for 24 hrs with mild agitation ...
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bioRxiv - Neuroscience 2020Quote: ... a lAAV200,000-labeled MOp mouse sample and a cslAAV20,000-labeled basal forebrain mouse sample were embedded with Technovit 9100 Methyl Methacrylate (MMA, Electron Microscopy Sciences, USA) according to previous procedures with minor optimizations66 ...
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bioRxiv - Molecular Biology 2019Quote: ... and labeled for 1 h with 20 µg/mL anti-PEG10 antibody diluted in EM blocking solution (EMS, Aurion). Samples were washed in PBS for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with 10 nm gold-conjugated goat anti-guinea pig IgG (1:200, Electron Microscopy Sciences, #25329) in blocking buffer for 3 hr ...
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bioRxiv - Cell Biology 2021Quote: Mouse inner ears were fixed in 4% paraformaldehyde (EMS Diasum) in PBS for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were cleaned by standard acetone precipitation (EM grade acetone; EMS). The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Developmental Biology 2021Quote: Mouse testes were fixed in 2.5% glutaraldehyde solution (EMS, Hatfield, PA, USA) in phosphate buffer (PB 0.1 M [pH 7.4] ...
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bioRxiv - Neuroscience 2023Quote: ... For primary mouse cortical neurons coverglass #1.5 (Electron Microscopy Sciences, Hatfield, PA) were coated for at least 16 hours at 37 °C ...
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bioRxiv - Immunology 2023Quote: Mouse tissue was dissected and fixed in 4% PFA (Electron Microscopy Sciences) before delivery to the Dana Farber/Harvard Cancer Center (DF/HCC ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Microbiology 2020Quote: ... Each protein was deposited on a CF-400-CU grid (Electron Microscopy Sciences) that had been plasma cleaned for 30 seconds in a Solarus 950 plasma cleaner (Gatan ...
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bioRxiv - Synthetic Biology 2020Quote: Purified protein samples applied to CF300-Cu carbon coated grids (Electron Microscopy Sciences) were prepared for negative stain transmission electron microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... mouse eyeballs were fixed o/n in 4% (w/v) PFA (171010; EMS) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Primary rat and mouse cardiomyocytes were fixed in 4% PFA (Electron Microscopy Sciences) for 10 min ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Neuroscience 2020Quote: ... and placed overnight in a 1:50 dilution of donkey anti–rabbit IgG with bound 10-nm colloidal gold (Electron Microscopy Sciences, catalog 25704) diluted in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... After that, Samples were infiltrated in graded mixtures (1:3, 1:1, 3:1) of resin (EMS, Resin Mixture ...
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bioRxiv - Neuroscience 2022Quote: Mouse brain coronal sections were mounted on plus slides (Catalogue number: EMS 71869-11) and dried for 2-3 hours on a slide drier ...
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bioRxiv - Bioengineering 2023Quote: The jejunum of a sacrificed mouse was fixed in 4% paraformaldehyde (Electron Microscopy Sciences), washed in PBS ...
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bioRxiv - Neuroscience 2024Quote: Mouse cochleae were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer (EMS, #15960) supplemented with 2 mM CaCl2 for 1.5-2 hours at room temperature ...