Labshake search
Citations for Electron Microscopy Sciences :
1 - 50 of 282 citations for Mouse Anti Hepatitis C Virus E2 Antibody 1876 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG/IgM-6nm gold secondary antibody (EMS) diluted 1:25 in 1% BSA PBS was added for 1.5 hours followed by another washing step ...
-
bioRxiv - Microbiology 2020Quote: ... the μl of virus solution were placed on glow discharged C-Flat 2/2 grids (EMS) and plunge frozen into liquid ethane using a Gatan Cryoplunge 3 ...
-
bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used were 6 nm anti-mouse immunogold particles (Electron Microscopy Sciences). Negative staining was carried out as previously described [17] ...
-
bioRxiv - Neuroscience 2024Quote: ... A secondary antibody (goat anti-mouse IgG conjugated to 15 nm colloidal gold; Electron Microscopy Sciences) diluted 1:20 in 0.1% BSA in PBS was added for 1.5 h ...
-
bioRxiv - Cell Biology 2023Quote: ... labeled with a secondary anti-rabbit or anti-mouse gold (depending on the primary antibody used) at RT for 1 hour (1:200 goat-anti-rabbit or goat-anti-mouse, Electron Microscopy Sciences), washed in 1% PBS with 3 sequential 50 µL drops ...
-
bioRxiv - Cell Biology 2019Quote: ... and 1:10 Goat-Anti-Mouse IgG (H+L) (EMS 25133) antibodies coupled with 6 nm and 15 nm gold particles ...
-
bioRxiv - Microbiology 2020Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
-
bioRxiv - Microbiology 2019Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
-
bioRxiv - Microbiology 2023Quote: ... followed by 12 nm gold conjugated goat anti-mouse IgG (Electron Microscopy Sciences) (1:20 dilution) ...
-
bioRxiv - Neuroscience 2023Quote: ... including Goat-anti-Human IgG (H&L) and Goat-anti-Mouse IgG (H&L) (Electron Microscopy Sciences, Hatfield, PA) (1:20 dilution in 1% BSA ...
-
bioRxiv - Microbiology 2023Quote: ... 3 µL of the purified CVB5 mutant virus sample at 4.5 mg mL-1 concentration was loaded onto Quantifoil R 1.2/1.3 grids (EMS), that were previously glow-discharged for 30 s in a GloCube Plus device (Quorum Technologies) ...
-
bioRxiv - Neuroscience 2020Quote: ... and 1 μL of virus mixed with Fast Green FCF Solution 2.5% (Electron Microscopy Sciences, Hatfield, PA, USA). The mouse was placed on a heating blanket (37 °C ...
-
bioRxiv - Cell Biology 2019Quote: ... incubated for 45 minutes with goat anti-rabbit 15nm gold antibody (EMS) diluted 1:20 in filtered blocking solution ...
-
bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
-
bioRxiv - Neuroscience 2020Quote: ... and filled with 0.5 µL of virus (titer ∼ 1012) dyed with Fast Green 2.5% (Electron Microscopy Sciences, Hatfield, PA, U.S.A.). The mice (∼8 weeks old ...
-
bioRxiv - Cell Biology 2023Quote: ... and incubated with anti-rabbit secondary antibody conjugated to gold particles (Electron Microscopy Sciences) in the blocking solution for 1 h ...
-
bioRxiv - Microbiology 2023Quote: ... The grids were washed twice with blocking buffer before labeling with secondary antibodies (goat anti-rat or goat anti-rabbit, 10nm, Electron Microscopy Sciences), rinsed three times in 1X PBS ...
-
bioRxiv - Microbiology 2020Quote: Four microliters of purified virus were applied to a Formvar-and carbon-coated 400-mesh copper grid (Electron Microscopy Sciences, Hatfield, PA) for 25 seconds ...
-
bioRxiv - Microbiology 2021Quote: Four μL of purified virus was applied to a Formvar- and carbon-coated 400-mesh copper grid (Electron Microscopy Sciences, Hatfield, PA) for 25 seconds ...
-
bioRxiv - Cell Biology 2021Quote: ... donkey anti-mouse IgG (H&L) conjugated with 15-nm gold particles (Electron Microscopy Science Aurion #25817, PA, USA).
-
bioRxiv - Microbiology 2021Quote: ... approximately 4 μL of purified virus sample was applied to a glow-discharged 300-mesh copper grid coated with continuous carbon film (Electron Microscopy Sciences, Hatfield, PA). The grid was plunged into a liquid ethane container that is further cooled by a liquid nitrogen bath ...
-
bioRxiv - Biophysics 2023Quote: U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
-
bioRxiv - Biochemistry 2021Quote: ... Quantifoil and C-flat grids (Electron Microscopy Sciences) were glow discharged with Pelco easiGlow for 60s at 25mA (negative polarity) ...
-
bioRxiv - Immunology 2022Quote: ... 1.2/1.3 C-Flat grid (Electron Microscopy Sciences). After 20 s of incubation ...
-
bioRxiv - Microbiology 2022Quote: ... Secondary antibodies used were goat-anti-Rabbit IgG conjugated to 10nm gold particles (Electron Microscopy Sciences, Cat. # 25109). We also labeled the samples with secondary gold-conjugated antibody alone to test for the non-specific labeling and found virtually no labeling in each case (data not shown) ...
-
bioRxiv - Neuroscience 2021Quote: ... and perfused transcardially with warm (~37 °C) Ames medium (MilliporeSigma) with heparin (MilliporeSigma) followed by warm (~37 °C) 2.5% glutaraldehyde (Electron Microscopy Sciences), 2% paraformaldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Bioengineering 2023Quote: ... the grids were incubated in a drop of Goat-anti-Mouse IgG coupled with 10 nm gold nanoparticles (Electron Microscopy Sciences, 25512) diluted at 1:100 in PBS containing 0.1% BSA for 1 h ...
-
bioRxiv - Molecular Biology 2019Quote: ... and labeled for 1 h with 20 µg/mL anti-PEG10 antibody diluted in EM blocking solution (EMS, Aurion). Samples were washed in PBS for 15 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... at 4 °C in 4% paraformaldehyde (PFA) (EMS, 15710). Then they were washed with PBS-Tween20 (0.1% ...
-
bioRxiv - Biophysics 2021Quote: ... C-flat holey carbon grids (CF-1.2/1.3-4Au, EMS) were glow-discharged for 20 s prior to the application of 3.5 μL of sample ...
-
bioRxiv - Biophysics 2022Quote: ... C-flat holey carbon grids (CF-1.2/1.3-4Au, EMS) were glow-discharged for 20 seconds prior to the application of 3.5 μL of sample ...
-
bioRxiv - Biophysics 2022Quote: ... C-flat holey carbon 1.2/1.3 grids (Electron Microscopy Sciences) or UltrAuFoil holey gold film grids (Ted Pella ...
-
bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
-
bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
-
bioRxiv - Neuroscience 2022Quote: Cells were fixed with 37°C 4% paraformaldehyde (Electron Microscopy Sciences) in 1X PBS for 15 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... or -20°C methanol with 1% paraformaldehyde (Electron Microscopy Sciences, 15710) for 10 minutes on ice ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...
-
bioRxiv - Cell Biology 2021Quote: ... and fixed overnight at 4°C with 4% Paraformaldehyde (PFA) (EMS #15710). Specimens (roughly 3-cm pieces ...
-
bioRxiv - Biophysics 2022Quote: C-flat™ EM (CF-2/1-4C-T, Electron Microscopy Sciences) grids were glow-discharged for 15 seconds on a Pelco Easiglow at 15mW power ...
-
bioRxiv - Molecular Biology 2022Quote: ... at 37°C and fixed in suspension in 4% paraformaldehyde (EMS 15714) with 0.0075% glutaraldehyde (EMS 16220 ...
-
bioRxiv - Microbiology 2023Quote: ... and embedded progressively at -20°C in LR Gold resin (EMS, USA). Resin polymerization was carried out at -20°C for 7 days under UV illumination ...
-
bioRxiv - Bioengineering 2022Quote: Degassed 2/2-3C C-flat grids (Electron Microscopy Sciences, Hatfield, PA) were glow discharged for 45 s at 30 mA ...
-
bioRxiv - Neuroscience 2020Quote: ... a lAAV200,000-labeled MOp mouse sample and a cslAAV20,000-labeled basal forebrain mouse sample were embedded with Technovit 9100 Methyl Methacrylate (MMA, Electron Microscopy Sciences, USA) according to previous procedures with minor optimizations66 ...
-
bioRxiv - Microbiology 2021Quote: ... Next, baked (60°C, overnight) CF300-Cu-UL EM grids (Electron Microscopy Sciences) were placed on top of the wells with the carbon side facing downwards ...
-
bioRxiv - Biophysics 2022Quote: C-Flat 2/2-3C or 1.2/1.3-3C grids (Electron Microscopy Sciences) were glow discharged at 10 mA for 90 seconds ...
-
bioRxiv - Immunology 2019Quote: ... samples were incubated overnight at 4°C in Uranyl Acetate Replacement (UA, EMS) in H2O (1:3) ...
-
bioRxiv - Neuroscience 2021Quote: ... slices were stored overnight at 4°C in 4% PFA (Electron Microscopy science) and moved to phosphate-buffered saline (PBS ...