Labshake search
Citations for Electron Microscopy Sciences :
51 - 100 of 151 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... 0.15 M NaCl) and adsorbed onto glow discharged carbon-coated Cu400 EM grids (Electron Microscopy Sciences) and blotted after 10 seconds ...
-
bioRxiv - Bioengineering 2023Quote: ... 4% Paraformaldehyde in 0.1 M phosphate buffer (15735-50S) was purchased from Electron Microscopy Sciences (PA). Ethanol absolute (64-17-5 ...
-
bioRxiv - Neuroscience 2024Quote: ... the brain underwent 2 washes with 0.2 M sodium cacodylate buffer (pH 7.2, Electron Microscopy Science), each lasting 10 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples were then washed with cold sodium cacodylate buffer 0.1 M and then postfixed in a reduced osmium solution (i.e., 1.5% potassium ferrocyanide (#20150 EMS) with 2% osmium tetroxide (#19170 EMS ...
-
bioRxiv - Neuroscience 2021Quote: ... retinae were fixed with glutaraldehyde (2.5% in 0.1 M cacodylate buffer at pH 7.4 for 2h at RT; Electron Microscopy Services-EMS) and rinsed with cacodylate buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... drop-fixed with 1% paraformaldehyde /2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) (Electron Microscopy Sciences), postfixed with 1% osmium tetroxide in 0.1 M cacodylate buffer (pH 7.4 ...
-
bioRxiv - Cell Biology 2019Quote: ... 8.5 µm, 6 µm, 6.5 µm, 6.5 µm, 6.5 µm for P0, AB, P1, ABa, ABp and EMS, respectively ...
-
bioRxiv - Neuroscience 2021Quote: ... Ileum and proximal colon were dissected out and gently flushed with 0.1 M sodium cacodylate buffer (EMS). The dissected segments were post-fixed overnight at 4°C in the fixative described above ...
-
bioRxiv - Cell Biology 2019Quote: Animals were transcardially perfused using 4% paraformaldehyde in 0.1 M sodium cacodylate (Electron Microscopy Sciences, Hatfield, PA) for 15 min at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... then washed in 0.1 M cacodylate buffer and post-fixed with 2% osmium tetroxide (Electron Microscopy Sciences) for a 1 h on ice ...
-
bioRxiv - Neuroscience 2023Quote: ... Animals were transcardially perfused initially with 0.1 M PBS followed by 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in 0.1 M PBS solution ...
-
bioRxiv - Microbiology 2023Quote: Fixed pellets were washed three times in 0.1% Ruthenium Red in 0.1 M cacodylate buffer (EMS #11650) and postfixed with 1% Osmium tetroxide (OsO4 ...
-
bioRxiv - Neuroscience 2024Quote: ... samples were post-fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2, EMS, #15960) supplemented with 2 mM CaCl2 for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... Fixed samples were washed three times by the addition of fresh 0.1 M cacodylate buffer (pH 7.2) buffer and post-fixed in 1% osmium (EMS) in 0.1 M cacodylate buffer (pH 7.2 ...
-
bioRxiv - Neuroscience 2021Quote: ... A small longitudinal incision was then made using a diamond dissecting blade (Type M-DL 72029 L; EMS) along the glue line either between two pharyngeal bulbs for AVL recording or in the tail region for DVB recording ...
-
bioRxiv - Molecular Biology 2021Quote: ... mutant G1-C1 strain obtained through chemical mutagenesis approach (0.4 M of EMS with 60 min exposure time) were cultured in AF-6 medium according to media recipe available in the Microbial Culture Collection National Institute for Environmental Studies (NIES-collection) ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then post-fixed with 1% OsO4 in Cacodylate buffer (0.1 M, pH 7.2, EMS, Hatfield, PA), rinsed in the same buffer and pelleted ...
-
bioRxiv - Neuroscience 2022Quote: ... IP) mice were paraformaldehyde-fixed (4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4, EMS Diasum, Hatfield, PA) through transcardial perfusion ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were fixed in 2% PFA/2.5% glutaraldehyde/0.1 M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Lateral ventricle walls were fixed for 18 h at 4 °C othen subdissected into anterior ...
-
bioRxiv - Neuroscience 2020Quote: ... unbuffered OsO4 aqueous solution for 2 hours and lead aspartate solution (0.03 M, pH 5.0 adjusted by KOH, EMS) at 50 °C for 2 hours ...
-
bioRxiv - Neuroscience 2019Quote: ... Larvae (96h AEL) were dissected in 0.1 M Sorensen’s Phosphate Buffer (SPB, Electron Microscopy Sciences, Hatfield, PA, USA), fixed with periodate-lysine-paraformaldehyde (PLP ...
-
bioRxiv - Neuroscience 2019Quote: ... then washed with 0.1 M PBS and coverslips were mounted on slides with Fluoromont-G (Electron Microscopy Sciences) solution with DAPI nuclear staining (Thermo-Fisher) ...
-
bioRxiv - Bioengineering 2019Quote: ... the samples were rinsed three times in 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences, Germany) for a total of 30 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... Mice were perfused with PBS containing 0.25 g/L heparin and 5 g/L procaine followed by a fixative solution containing 2.5% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4) (#1549, Electron Microscopy Science). Brains were dissected and post-fixed overnight in a fixative solution at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... A small longitudinal incision was then made using a diamond dissecting blade (Type M-DL 72029 L; EMS) along the glue line adjacent to the neuron of interest ...
-
bioRxiv - Neuroscience 2021Quote: ... then anesthetized in 0.12% tricaine in E3 and immediately fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences) supplemented with 2 mM CaCl2 ...
-
bioRxiv - Developmental Biology 2019Quote: ... gmIs18 or vab-8(e1017)gmIs18 worms were mutagenized for 4 hours by incubation in 0.05 M ethyl methane sulfonate (EMS). Worms were washed in M9 buffer (22mM KH2P04 ...
-
bioRxiv - Plant Biology 2020Quote: ... 2.5% in phosphate buffer (PB 0.1 M [pH 7.4]) for 1h at RT and post-fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma ...
-
bioRxiv - Plant Biology 2020Quote: ... 2.5% in phosphate buffer (PB 0.1 M [pH 7.4]) for 1h at RT and post fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... cells at DIV 21 were washed with PBS and fixed overnight in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... aeruginosa strain PAK ΔflhF attB::flhF(R251G) was divided and treated with either 0.1 M ethyl methane sulfonate (EMS), 0.1 mg/ml methylnitronitrosoguanidine (NG) ...
-
bioRxiv - Cell Biology 2020Quote: N2 worms were mutagenized for 4 h by incubation in the presence of 0.05 M ethyl methane sulfonate (EMS) according to the standard protocol (24) ...
-
bioRxiv - Biophysics 2022Quote: ... media was removed from the plates and immediately fixed using 2.5% Glutaraldehyde 2.5% Paraformaldehyde in 0.1 M sodium cacodylate buffer at pH 7.4 (Electron Microscopy Sciences, 15949) for at least 1 hour at room temperature before embedding.
-
bioRxiv - Molecular Biology 2023Quote: ... Animals were incubated overnight in fixation buffer (2.5% glutaraldehyde, 1.0% paraformaldehyde, and 0.1 M sodium phosphate (Electron Microscopy Sciences)) at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Fixative was removed by washing 5 x 3 min in 0.1 M cacodylate buffer and samples were incubated in 2% osmium (OsO4, EMS) in 0.1 M cacodylate buffer for 30 min at RT ...
-
bioRxiv - Microbiology 2024Quote: ... The samples were then rinsed with 0.1 M cacodylate buffer and fixed with 1 % osmium tetroxide (Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer for 30 min at room temperature ...
-
bioRxiv - Biophysics 2024Quote: ... followed by embedding in 2% agarose and post-fixed in 0.1 M osmium tetroxide (SKU 19152, Electron Microscopy Science). Dehydration was achieved through a series of acetone solutions with increasing concentrations ...
-
bioRxiv - Cell Biology 2022Quote: Human neurons were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 15 minutes at room temperature ...
-
bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
-
bioRxiv - Cell Biology 2022Quote: ... The tissues were cut into small pieces of about 2 mm2 and then fixed by 0.1 M phosphate buffer (pH 7.4) including 2% PFA (Electron Microscopy Sciences) and 2% glutaraldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Neuroscience 2019Quote: ... 18 larva of the two different ages were fixed whole in Karnovsky’s fixative (2.5% glutaraldehyde and 2% paraformaldehyde solution diluted in 0.1 M cacodylate buffer at pH 7.4; EMS RT15720; Karnovsky, 1965) for at least 24 hours at 15-20 °C and at a pH of 7.2 ...
-
bioRxiv - Microbiology 2019Quote: ... The pellet was resuspended in buffer (0.2 M HEPES, pH 7.4, 5 mM CaCl2) and bacteria were fixed by addition of glutaraldehyde (Electron Microscopy Sciences) in buffer to a final concentration of 2.5% for 1 h at 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 μl of 10 μM GMPCPP-MT (polymerised as described above) were applied to a glow-discharged C-flat 2/2-4C grid (EMS) at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: After 8 washes with 0.1 M cacodylate buffer at pH 7.4 (SigmaAldrich) the tissue was post fixed by 1% osmium tetroxide (Electron Microscopy Sciences) and 0.6% K3Fe(CN)6 in a 0.1 M cacodylate buffer for 1 hr ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed in water and then maleate buffer (MilliporeSigma) (pH=6.0) and stained in 0.05 M Maleate Buffer containing 1% uranyl acetate (Electron Microscopy Sciences) at 4°C overnight ...
-
bioRxiv - Bioengineering 2019Quote: ... agarose RoCs with ROs and RPE were fixed in the chambers with Karnovsky buffer (2.5% glutaraldehyde, 2% paraformaldehyde, 0.1 M sodium cacodylate buffer, pH 7.4) (Electron Microscopy Sciences, Germany) for 12 hours at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: Organoids were fixed in 3% glutaraldehyde and 1% paraformaldehyde in 0.08 M sodium acodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... gently washed in 0.1 M phosphate buffer (PB) and fixed in 3.5% EM-grade glutaraldehyde (Electron Microscopy Sciences; catalog 16210) in 0.1M Phosphate buffer (PB ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were fixed overnight at 4°C in freshly prepared phosphate buffer (0.1 M, pH 7.4) containing 4% paraformaldehyde (15700, Electron Microscopy Sciences) and 0.2% glutaraldehyde (16200 ...