Labshake search
Citations for Electron Microscopy Sciences :
1 - 50 of 112 citations for L Leucine N T Boc H2O 13C6 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... and leucine (20 mg/L) were performed to examine the effect of ethyl methanesulfonate (EMS), sodium dodecyl sulfate (SDS) ...
-
bioRxiv - Neuroscience 2020Quote: ... cleared spinal cords and whole-mount skin samples were mounted on glass slides in 97% TDE using press-to-seal silicone isolators (Electron Microscopy Sciences, Hatfield, PA, USA) with the dorsal surface facing up.
-
bioRxiv - Neuroscience 2023Quote: ... and mounted on a poly-L-lysine coated coverslip using Fluoromont-G T (Electron Microscopy Science, USA). Alexa Fluor conjugated secondary antibodies (Alexa Fluor 488 conjugated goat anti-guinea pig ...
-
bioRxiv - Plant Biology 2021Quote: ... glued with conductive resin Epotek H2OS® (EMS, Hatfield, PA, US), and polymerized overnight in an oven at 60°C ...
-
bioRxiv - Cell Biology 2019Quote: HEK293 Flp-ln T-REx cells were grown on poly-L-lysine-coated #1.5 glass coverslips (Electron Microscopy Sciences). Cells were treated with 1 μg/mL doxycycline for 24 h to induce expression of BirA* or BirA*-CNAα ...
-
bioRxiv - Immunology 2019Quote: ... After washing in H2O samples were incubated in 1% Thiocarbohydrazide (TCH, EMS) in water for 20 minutes ...
-
bioRxiv - Bioengineering 2023Quote: A 80 × 50 × 20 mm (L × W × T) block of PMMA (Acrylic Plastic 24210-08, Electron Microscopy Sciences, Hatfield, PA 19440, USA) was cast and cured in a mold for 24 hours ...
-
bioRxiv - Immunology 2019Quote: ... Mouse lungs were inflated to 15 cm of H2O and fixed with 4% paraformaldehyde (EMS) for 24 h ...
-
Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytesbioRxiv - Cell Biology 2023Quote: ... the cells were treated with 1% OsO4 in H2O (Electron Microscopy Sciences, Hatfield Pennsylvania, United States) for 30 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... Mouse lungs were inflated to 15 cm H2O and fixed with 4% paraformaldehyde (EMS, Hatfield, PA, USA) for 24 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... slides were washed in Maxpar H2O and then incubated with 0.0005% Ruthenium (RuO4)/PBS (Electron Microscopy Sciences)40 for 3 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... Glutaraldehyde (Electron Microscopy Science, cat n°16220). The following primary antibodies were used in this study ...
-
bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
-
bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
-
bioRxiv - Microbiology 2021Quote: ... 0.1% uranyl acetate and 2% H2O followed by en bloc Osmium-Thiocarbohydrazide-Osmium staining75,76 and embedding in Durcupan ACM (Electron Microscopy Sciences). Serial block-face imaging was conducted in a Zeiss Sigma 3View system with variable pressure with 30 nm x 70 nm resolution at 5.5 kV ...
-
bioRxiv - Biochemistry 2022Quote: ... Three washing steps with double distilled H2O were followed by three staining steps with 0.75% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were imaged using a FEI Tecnai 10 operated at 100 keV ...
-
bioRxiv - Neuroscience 2022Quote: ... Grids were washed with two drops of double-distilled H2O and stained with two drops of freshly prepared 1% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were analysed at room temperature using a Tecnai Spirit (FEI ...
-
bioRxiv - Immunology 2021Quote: ... 48-hour activated T cells from PRMT5fl/fl and T-PRMT5Δ/Δ mice were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min in V-bottom plates (Costar ...
-
bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
-
bioRxiv - Developmental Biology 2021Quote: ... resuspended in 100 mM sucrose and the cell suspension distributed onto slides (Matsunami, S9901) covered with 1% PFA in H2O (Electron Microscopy Science, 15713) with 0.2% Triton X-100 (pH 9.2-9.4) ...
-
bioRxiv - Neuroscience 2021Quote: Coronal sections (n=2 mice, P30) and whole mounts (n=2 mice, P28) were incubated with Immunosaver (1:200; EMS, Fort Washington, PA) for 20 min at 60°C ...
-
bioRxiv - Biophysics 2022Quote: C-flat™ EM (CF-2/1-4C-T, Electron Microscopy Sciences) grids were glow-discharged for 15 seconds on a Pelco Easiglow at 15mW power ...
-
bioRxiv - Neuroscience 2023Quote: ... including Goat-anti-Human IgG (H&L) and Goat-anti-Mouse IgG (H&L) (Electron Microscopy Sciences, Hatfield, PA) (1:20 dilution in 1% BSA ...
-
bioRxiv - Neuroscience 2020Quote: ... Cat N° SND75 (microgroove length: 75 μm) and Cat N° XC150 (microgroove length: 150 μm)) and 7.8mil Aclar 33C sheets (Electron Microscopy Sciences, Hatfield, PA, USA, Cat N° 50425-25) were sterilized in 70% ethanol and left to air-dry in the laminar flow ...
-
bioRxiv - Cell Biology 2022Quote: ... poly-L-lysine coated glass coverslips (#1.5, EMS) were placed into tissue culture treated 12-well plates and washed twice with growth medium and cells were seeded at 1.5 x 105 per well ...
-
bioRxiv - Genetics 2022Quote: ... Then C→T or G→A substitutions which are usually induced by EMS mutagenesis ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse eyeballs were fixed o/n in 4% (w/v) PFA (171010; EMS) at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... After washing with TBS-T and staining with Uranyless (Cat# 22409, Electron Microscopy Sciences), TEM imaging was conducted at the Biotron Experimental Research Center ...
-
bioRxiv - Cancer Biology 2020Quote: HEKRON/EGFR cells were plated onto glow-discharged (EMS 150 T ES, Quorum Technologies), PLL coated glass coverslips overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... washed and fixed O/N in 4% paraformaldehyde/phosphate buffered saline (PBS) solution (EMS). Left lung lobes were then treated for paraffin inclusion ...
-
bioRxiv - Cell Biology 2021Quote: ... myoblasts were plated on poly-L-lysine coated coverslips (EMS) at 70% confluency and cultured overnight.
-
bioRxiv - Neuroscience 2023Quote: ... or a poly-L- lysine coated coverslip (Electron Microscopy Sciences), are fed twice weekly with 1mL neurobasal-A media supplemented with 500μM Glutamax ...
-
bioRxiv - Cancer Biology 2020Quote: Cells cultured on coverslips were fixed in 4% PFA (Electron Microscopy Sciences, Cat. N. 15710) in PBS for 10 min followed by incubation in blocking solution for 1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were fixed with PFA 4% in PBS 1X (Electron Microscopy Sciences, cat n°15700) for 10 min ...
-
bioRxiv - Plant Biology 2021Quote: ... then for G/C to A/T transitions that were likely to be caused by EMS, and thirdly for non-synonymous amino-acid changes.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed again with PBS-T and mounted in Fluoro Gel with DABCO™ (Electron Microscopy Science) before visualizing with either a Leica DM2500 microscope equipped with a Leica DFC 7000T camera and a LAS X software or with a Zeiss 710 confocal microscope with Zen software (Zeiss) ...
-
bioRxiv - Cell Biology 2019Quote: ... and 1:10 Goat-Anti-Mouse IgG (H+L) (EMS 25133) antibodies coupled with 6 nm and 15 nm gold particles ...
-
bioRxiv - Bioengineering 2022Quote: ... Both channels (n=3 per donor) were fixed using 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences). The PFA solution was pipetted into both channels followed by a 15-minute incubation at room temperature (RT) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... post fixed them for 2 hours in 1% osmium tetroxide (Electron Microscopy Sciences, cat n. 19150) 0.2 cacodylate sucrose buffer and washed them 1 time with buffer ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We stained them en bloc with 1% aqueous uranyl acetate (Electron Microscopy Sciences, cat n. 22400) for 16 hours at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... 0.05% NP-40) to glow-discharged C-Flat holey carbon grids (CF-2/1-3C-T, EMS). The samples were plunge-frozen using a Vitrobot (ThermoFisher ...
-
bioRxiv - Genomics 2024Quote: ... samples were washed twice with PBS-T prior to post-fixation in 3% formaldehyde (Electron Microscopy Sciences 15714) and 0.1% glutaraldehyde (Electron Microscopy Sciences 16120 ...
-
bioRxiv - Genetics 2019Quote: ... Subsequent analysis concentrated on the 1,854 transition variants (C to T or G to A) as these were likely caused by EMS mutagenesis ...
-
bioRxiv - Biochemistry 2021Quote: ... Ycf1 sample was applied to a CFlat-1.2/1.3-4C-T (WT Ycf1) or QF-1.2/1.3-4Au grid (E1435Q Ycf1) purchased from Electron Microscopy Sciences. Grids were placed inside of a Leica EM GP2 equilibrated to 10°C and 80% humidity ...
-
bioRxiv - Plant Biology 2022Quote: ... approximately 40,000 Arabidopsis seeds of a homozygous coi1-8 mutant (also homozygous for the T locus) were treated with ethyl methane sulfonate (EMS) and sown on soil (M1 generation) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...
-
bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
-
bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
-
bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
-
bioRxiv - Neuroscience 2023Quote: Mice (female; n=4) at 15 dpl were perfused with PFA prepared in 0.1 M Sorensen’s buffer (Electron Microscopy Sciences). The spinal cords were harvested ...