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Citations for Electron Microscopy Sciences :
1 - 50 of 79 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... glued with conductive resin Epotek H2OS® (EMS, Hatfield, PA, US), and polymerized overnight in an oven at 60°C ...
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bioRxiv - Cell Biology 2024Quote: ... washed with H2O and stained with 1% (w/v) uranyl acetate (Electron Microscopy Sciences #22400) or formate (Electron Microscopy Sciences #22450) ...
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Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytesbioRxiv - Cell Biology 2023Quote: ... the cells were treated with 1% OsO4 in H2O (Electron Microscopy Sciences, Hatfield Pennsylvania, United States) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse lungs were inflated to 15 cm H2O and fixed with 4% paraformaldehyde (EMS, Hatfield, PA, USA) for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed in Maxpar H2O and then incubated with 0.0005% Ruthenium (RuO4)/PBS (Electron Microscopy Sciences)40 for 3 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% uranyl acetate and 2% H2O followed by en bloc Osmium-Thiocarbohydrazide-Osmium staining75,76 and embedding in Durcupan ACM (Electron Microscopy Sciences). Serial block-face imaging was conducted in a Zeiss Sigma 3View system with variable pressure with 30 nm x 70 nm resolution at 5.5 kV ...
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bioRxiv - Biochemistry 2022Quote: ... Three washing steps with double distilled H2O were followed by three staining steps with 0.75% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were imaged using a FEI Tecnai 10 operated at 100 keV ...
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bioRxiv - Neuroscience 2022Quote: ... Grids were washed with two drops of double-distilled H2O and stained with two drops of freshly prepared 1% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were analysed at room temperature using a Tecnai Spirit (FEI ...
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bioRxiv - Neuroscience 2024Quote: ... made in double distilled H2O (ddH2O) and crosslinked by 1% glutaraldehyde (diluted from 50% glutaraldehyde, Electron Microscopy Sciences, catalog #16320, Hatfield, PA). 40 µm sections were cut on a vibratome (Leica VT1000S ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in 100 mM sucrose and the cell suspension distributed onto slides (Matsunami, S9901) covered with 1% PFA in H2O (Electron Microscopy Science, 15713) with 0.2% Triton X-100 (pH 9.2-9.4) ...
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bioRxiv - Neuroscience 2023Quote: ... including Goat-anti-Human IgG (H&L) and Goat-anti-Mouse IgG (H&L) (Electron Microscopy Sciences, Hatfield, PA) (1:20 dilution in 1% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... poly-L-lysine coated glass coverslips (#1.5, EMS) were placed into tissue culture treated 12-well plates and washed twice with growth medium and cells were seeded at 1.5 x 105 per well ...
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bioRxiv - Genomics 2024Quote: ... poly-L-lysine coated microscope slides (Electron Microscopy Sciences). Confluent cell cultures were trypsinized and resuspended in 200-500 µL growth media such that 50,000-100,000 cells could be applied to the four middle wells (within the CosMx scanning area ...
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bioRxiv - Cell Biology 2021Quote: ... myoblasts were plated on poly-L-lysine coated coverslips (EMS) at 70% confluency and cultured overnight.
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bioRxiv - Neuroscience 2024Quote: ... or a poly-L-lysine coated coverslip (Electron Microscopy Sciences), are fed twice weekly with 1mL neurobasal-A media supplemented with 500μM Glutamax ...
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bioRxiv - Genomics 2024Quote: ... Poly-L lysine-coated coverslips were used (Electron Microscopy Sciences, Cat #72292-01) and 200.000 cells were seeded per coverslip ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown on poly-L-lysine-coated #1.5 glass coverslips (Electron Microscopy Sciences). Cells were fixed with −20°C methanol for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: Cells were plated and cultured on coverslips pretreated with Poly-L-lysine (Electron Microscopy Sciences), then fixed with formaldehyde 1% and permeabilized with Igepal 0.1% ...
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bioRxiv - Neuroscience 2021Quote: ... 8 µm – 10 µm cryosections were collected on poly-L-lysine coated glass slides (EMS), and unfixed eye cups sections were immediately fixed with 2% PFA for 2 minutes ...
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bioRxiv - Genetics 2023Quote: ... and leucine (20 mg/L) were performed to examine the effect of ethyl methanesulfonate (EMS), sodium dodecyl sulfate (SDS) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were seeded onto Poly-L-lysine-coated glass coverslips (Electron Microscopy Science, 72292-08) in a 24-well plate format ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Seeds were collected from a single Columbia founder and treated with 20 μmol/L ethylmethane sulfonate (EMS) for 12 hrs ...
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bioRxiv - Neuroscience 2023Quote: ... and mounted on a poly-L-lysine coated coverslip using Fluoromont-G T (Electron Microscopy Science, USA). Alexa Fluor conjugated secondary antibodies (Alexa Fluor 488 conjugated goat anti-guinea pig ...
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bioRxiv - Neuroscience 2021Quote: ... A small longitudinal incision was then made using a diamond dissecting blade (Type M-DL 72029 L; EMS) along the glue line either between two pharyngeal bulbs for AVL recording or in the tail region for DVB recording ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown on poly-L-lysine coated coverslips and fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) for 10 min at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: TSCs and TGCs were grown on poly-L-lysine–coated #1.5 glass coverslips (Electron Microscopy Sciences, Hatfield, PA) for confocal microscopy ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to counterstain sections and coverslips were mounted using Fluorogel l (Electron Microscopy Sciences, Hatfield, PA, USA). Confocal Microscopy image acquisition was taken on a LSM 710 microscope (Zeiss ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Bioengineering 2022Quote: ... followed by approximately 1 L of 4% (w/v) paraformaldehyde (PFA) fixative (19208, Electron Microscopy Sciences, Hatfield, PA) in 1X PBS.
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bioRxiv - Neuroscience 2022Quote: ... Cells were then plated as attached OPCs on 0.01% Poly-L-Lysine coated plates (Electron Microscopy Sciences, 19320-B) in the same media ...
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bioRxiv - Cell Biology 2021Quote: ... donkey anti-mouse IgG (H&L) conjugated with 15-nm gold particles (Electron Microscopy Science Aurion #25817, PA, USA).
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells plated on glass bottom dishes and platelets immobilized on poly-L-lysine (Electron Microscopy Sciences, Hatfield, PA)-coated on glass bottom dishes were fixed with 4.0 % paraformaldehyde (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μL of the collected hemolymph was applied to poly-L-lysine slides (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to rest for ten minutes at room temperature on a flat surface (Fig ...
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bioRxiv - Bioengineering 2022Quote: ... the first microfluidic device was placed on a clean poly-L-lysine coated slide (PLL slide, Electron Microscopy Science) and baked for 2 hours at 75°C oven to strengthen the bonding ...
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bioRxiv - Biochemistry 2020Quote: 5 μL of purified sample was applied to glow-discharged (10s) Quantifoil R 1.2/1.3 grids (Au 400; Electron Microscopy Sciences) and plunge-frozen in liquid nitrogen-cooled liquid ethane ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Immunology 2021Quote: ... A 4-μl drop of the diluted sample was applied to a freshly glow-discharged carbon-coated copper grid (400-mesh, EMS) for approximately one minute ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Bioengineering 2023Quote: 5 μL of D-GQDs/exo loaded on the Poly-L-Lysine coated slides (63410; Electron Microscopy Sciences, PA, USA) and was fully covered with 18 mm × 18 mm square cover glasses (2845-18 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 µL of protein sample were applied to freshly glow discharged Quantifoil R1.2/1.3 Cu 200 mesh grids (EMS, Q225-CR1.3) and were frozen by plunging into liquid ethane using an FEI Vitrobot Mark IV (100% humidity ...
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bioRxiv - Neuroscience 2024Quote: ... coronal cryosections of 10 μm were prepared on poly-L-lysine-coated glass slides (no. 63478-AS, Electron Microscopy Sciences) or 50×75 mm poly-L-lysine-coated glass slides ...
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bioRxiv - Biophysics 2024Quote: ... by applying 3 μl of purified XPR1 to a glow-discharged holy carbon gold QUANTIFOIL 1.2/1.3 grid (400 mesh, Electron Microscopy Sciences) and blotting for 4 to 5 s at 4°C and 100% humidity ...
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bioRxiv - Cell Biology 2020Quote: ∼5-6μl of the resuspended 100,000 ×g pellet fraction was spread onto glow discharged Formvar-coated copper mesh grids (Electron Microscopy Sciences) and stained with 2% Uranyl acetate for 2 min ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
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bioRxiv - Microbiology 2020Quote: ... purified EVs were resuspended in 30 μL D-MEM (0% FCS) and mixed with a low volume of Glutaraldehyde 25% (final concentration = 2.5%) (16220, Electron Microscopy Sciences). After 10 min of fixation at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...