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Citations for Electron Microscopy Sciences :
51 - 99 of 99 citations for L Alanine N T Boc 13C3 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... followed by approximately 1 L of 4% (w/v) paraformaldehyde (PFA) fixative (19208, Electron Microscopy Sciences, Hatfield, PA) in 1X PBS.
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to counterstain sections and coverslips were mounted using Fluorogel l (Electron Microscopy Sciences, Hatfield, PA, USA). Confocal Microscopy image acquisition was taken on a LSM 710 microscope (Zeiss ...
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bioRxiv - Neuroscience 2023Quote: ... A small longitudinal incision was then made using a diamond dissecting blade (Type M-DL 72029 L; EMS) along the glue line adjacent to the neuron of interest ...
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bioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with ketamine/xylazine and transcardially perfused with PBS followed by PFA (4% in PBS; Electron Microscopy Sciences, cat n°15700). Extracted brains were post-fixed (PFA 4% overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then plated as attached OPCs on 0.01% Poly-L-Lysine coated plates (Electron Microscopy Sciences, 19320-B) in the same media ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were grown on 22mm glass coverslips (#1.5) pre-treated with 0.01% poly-L-lysine (Electron Microscopy Sciences) and then coated with 10μg/ml laminin (Cultrex ...
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bioRxiv - Cell Biology 2021Quote: ... donkey anti-mouse IgG (H&L) conjugated with 15-nm gold particles (Electron Microscopy Science Aurion #25817, PA, USA).
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bioRxiv - Bioengineering 2022Quote: ... the first microfluidic device was placed on a clean poly-L-lysine coated slide (PLL slide, Electron Microscopy Science) and baked for 2 hours at 75°C oven to strengthen the bonding ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μL of the collected hemolymph was applied to poly-L-lysine slides (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to rest for ten minutes at room temperature on a flat surface (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells plated on glass bottom dishes and platelets immobilized on poly-L-lysine (Electron Microscopy Sciences, Hatfield, PA)-coated on glass bottom dishes were fixed with 4.0 % paraformaldehyde (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μL of the collected hemolymph was applied to poly-L-lysine slides (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to rest for ten minutes at room temperature on a flat surface ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times with PBS (pH 7.4) and mounted in slides cover with gelatin using Fluoromount-G (Electron Microscopy Sciences, cat n° 17984-25).
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bioRxiv - Biochemistry 2020Quote: 5 μL of purified sample was applied to glow-discharged (10s) Quantifoil R 1.2/1.3 grids (Au 400; Electron Microscopy Sciences) and plunge-frozen in liquid nitrogen-cooled liquid ethane ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Immunology 2021Quote: ... A 4-μl drop of the diluted sample was applied to a freshly glow-discharged carbon-coated copper grid (400-mesh, EMS) for approximately one minute ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Bioengineering 2023Quote: 5 μL of D-GQDs/exo loaded on the Poly-L-Lysine coated slides (63410; Electron Microscopy Sciences, PA, USA) and was fully covered with 18 mm × 18 mm square cover glasses (2845-18 ...
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bioRxiv - Cell Biology 2020Quote: ∼5-6μl of the resuspended 100,000 ×g pellet fraction was spread onto glow discharged Formvar-coated copper mesh grids (Electron Microscopy Sciences) and stained with 2% Uranyl acetate for 2 min ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
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bioRxiv - Microbiology 2020Quote: ... purified EVs were resuspended in 30 μL D-MEM (0% FCS) and mixed with a low volume of Glutaraldehyde 25% (final concentration = 2.5%) (16220, Electron Microscopy Sciences). After 10 min of fixation at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... For immunofluorescence analysis cells were seeded (1.5 x 105 HeLa cells) directly on poly-L-lysine coated coverslips (Electron Microscopy Sciences), which were placed in a 12-well plate ...
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed thrice with PBS and smear dried on a Poly-L-lysine coated slide (Electron Microscopy Science: 63410), mounted with Prolong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: A drop (1-2 μl) of undiluted EVs (PEG/SEC-enriched EVs from 100 uninjured egfp(RNAi) animals) was placed on a formvar (EMS 15810)-coated hexagonal grid (EMS G300-Cu ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 2 % PFA and allowed to attach on poly-L-lysine-coated 12 mm glass coverslips (Electron Microscopy Sciences, Hatfield, Pennsylvania) for 30 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... sectioned (thickness of 7-10 µm) and put at the center of poly-L-lysine covered glass slides (63478-AS, Electron Microscopy Sciences).
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bioRxiv - Biochemistry 2020Quote: ... An aliquot (approximately 4 μL) of diluted sample was placed on an ultra-thin carbon-coated 200-mesh copper grid (CF200-Cu-UL, Electron Microscopy Sciences, Hatfield ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide fibrils were obtained as described a ove and 5 μl of the sample was spotted on non-glow discharged carbon film 300 mesh copper grids (CF300-CU from Electron Microscopy Sciences) and incubated for 1 min ...
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bioRxiv - Biochemistry 2019Quote: ... were prepared by applying 3 – 5 μL of in vitro assembled PLP (with or without PC portal rings) onto 300-mesh carbon coated copper grids (Electron Microscopy Sciences). Samples were absorbed for 1 minute and washed with 2-3 drops of water ...
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bioRxiv - Biophysics 2020Quote: 4 μl of 22 μM tau aggregation samples were adsorbed onto glow-discharged 200 mesh formvar carbon coated copper grids (Electron Microscopy Sciences) and stained with aqueous uranyLess (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: Purified eCIS samples were briefly centrifuged to remove impurities and 5 μL of supernatant was applied onto freshly glow discharged carbon-coated copper grids (CF400-CU, 6 nm, 400 mesh, Electron Microscopy Sciences). Samples were allowed to adsorb for 2 minutes before being blotted off by filter paper and grids were washed twice with filtered distilled water ...
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bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...
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Design and Evaluation of Nanoscale Materials with Programmed Responsivity towards Epigenetic EnzymesbioRxiv - Bioengineering 2024Quote: A drop of nanoparticle sample obtained from the self-assembly of PEG-block-poly (acetylated L-lysine) co-polypeptide was placed on a 300-mesh Formvar carbon-coated copper TEM grid (Electron Microscopy Sciences) for 1 min and wicked off ...
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bioRxiv - Biophysics 2024Quote: 3 µL diluted MgtA (0.01 mg/mL) was deposited on a carbon-coated 400 mesh copper grid (CF400-CU, EMS, Hatfield, PA, USA) following glow discharging (PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μL of sample solution was applied onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS, Hatfield, PA, USA). The sample solution was left for 30 s before blotted with a piece of filter paper ...
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bioRxiv - Genetics 2020Quote: ... Tissue was transferred to a drop of Schneider’s on a silanized coverslip and gently squashed onto a Poly-L-Lysine coated slide (Electron Microscopy Sciences, Hatfield, PA). Coverslips were immediately removed after freezing in liquid nitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... 4μL of ~1 nM purified DNA nanostructure was deposited onto a copper TEM grid coated with carbon and formvar (Electron Microscopy Sciences, Hartfield, PA) and incubated for 4 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... A 5 μL sample of the peak fraction was then added to glow discharged copper CF400-CU EM grids (cat. # 71150, Electron Microscopy Sciences, Hatfield, PA) for 1 min ...
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bioRxiv - Genomics 2022Quote: ... OCT tissue blocks were sectioned into 10 µm thickness sections and placed in the center of poly-L-lysine slides (Electron Microscopy Sciences, 63478-AS), and shipped with dry ice ...
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bioRxiv - Cancer Biology 2023Quote: 10 μl of the isolated amyloid fibril obtained as mentioned above were spotted on to a copper-coated formvar grids (Electron Microscopy Sciences, Hatfield, PA, USA). The grid was washed with MQ water and stained for 20 min with 10 μl 0.1% uranyl formate solution (Electron Microscopy Sciences) ...
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bioRxiv - Bioengineering 2023Quote: ... 4 µl of AAV9 capsids containing GFP or Cas9 cargo was deposited on 2/2 400 mesh C-flat grids (Electron Microscopy Sciences, Hatfield, PA, #CF224C-50), which were previously glow discharged at 15 mA for 15 s on PELCO easyGLOW instrument ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated overnight at 4°C in a 1:100 dilution of Goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8 nm colloidal gold (Aurion, Electron Microscopy Sciences, Fort Washington, PA, cat #25121-0.6ml). Following washes in IEM buffer ...