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Citations for Electron Microscopy Sciences :
551 - 600 of 688 citations for Borrelia burgdorferi sensu stricto B31 CRASP 2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: BF PolIE RNAi cells were grown with or without doxycycline for 24 hours then incubated with 2 mM of ethylmethanesulfonate (EMS; Sigma) or DMSO (vehicle ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3 μl of the particle dispersion was applied on 400-mesh C-Flat CF-2/1-4C copper grids (Protochips, USA) negatively glow discharged with a Plasma Cleaner (EMS, USA) at 50 mA for 45 s ...
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bioRxiv - Genomics 2019Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Zoology 2021Quote: Sets of oocytes including some number of visibly infected individuals were fixed in 2% glutaraldehyde in 0.2 M sodium cacodylate buffer (SCB, Electron Microscopy Sciences) for one hour and post-fixed in 2% osmium tetroxide in SCB for one hour (Bozzola and Russell ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% uranyl acetate and 2% H2O followed by en bloc Osmium-Thiocarbohydrazide-Osmium staining75,76 and embedding in Durcupan ACM (Electron Microscopy Sciences). Serial block-face imaging was conducted in a Zeiss Sigma 3View system with variable pressure with 30 nm x 70 nm resolution at 5.5 kV ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on bone slices for 3 days were unroofed as described above and fixed for 10 min in a 0.2M sodium cacodylate buffer (pH 7.4) containing 2% paraformaldehyde (Electron Microscopy Sciences 15710) and 2.5% glutaraldehyde (Electron Microscopy Sciences 16220) ...
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bioRxiv - Plant Biology 2021Quote: ... The roots of 7 to 10 day old seedlings were collected and fixed for 30 min with 2-(N-morpholino) ethane sulfonic acid (MES) buffer solution pH 5.7 containing 4% paraformaldehyde (32% methanol-free solution; Electron microscopy Sciences). pH was adjusted to 5.7 with KOH solution.
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 3.2 μL of RNCs were incubated on plasma-cleaned 300-mesh holey carbon grids (C-flat R2/2, Electron Microscopy Science) for 1 min ...
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bioRxiv - Biophysics 2020Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μl of diluted sample was deposited onto a glow-discharged (Edwards S150B, 30sec, 30-50 mA, 1,2kV, 10-2 mbar) continuous carbon grid (CF400-CU-UL, Electron Microscopy Sciences). After one minute at RT ...
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bioRxiv - Biophysics 2022Quote: ... then cells were allowed to adhere for 15 min in media prior to chemical fixation in 2% PFA and 0.2% glutaraldehyde (Electron Microscopy Sciences). FActin was stained by permeabilizing cells with 0.1% Triton-X-100 prior to incubation with 3.3 μM phalloidin-AlexaFluor647 (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: Marchantia thalli were cut in small pieces (1×2 mm) and fixed immediately in buffered phosphate 0,1 M paraformaldehyde 4% (Electron Microscopy Sciences), glutaraldehyde 2,5% (TAAB Laboratories ...
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bioRxiv - Neuroscience 2021Quote: Glass vials were prepared prior to the experiments with a 2-mm layer of Sylgard 184 (Electron Microscopy Sciences, Hatfield, PA) at the bottom ...
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bioRxiv - Neuroscience 2020Quote: ... 20 mM NaCl and incubated with secondary 15 nm gold-tagged goat-anti-rabbit IgG F(ab’)2 (Electron Microscopy Sciences) diluted 1:10 in 20 mM Tris pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were taken prior to the experiment (‘Pre’) and at each time point by diluting 50 μl of cells 1:1 with 2% glutaraldehyde (EMS, 16220) and incubating at 4°C to allow cells to sediment ...
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bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
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bioRxiv - Biophysics 2019Quote: 5 uL of pCV2-2 VLP samples was applied to CF200-CU carbon film 200 mesh copper grids (Electron Microscopy Science) for 1 min and the grids were then washed with 200 µl of 50 mM of Na Cacodylate buffer and then strained immediately with 50 µl of 0.5% uranyl acetate for 1 min ...
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bioRxiv - Neuroscience 2019Quote: 63 day old hMO specimens were immersion-fixed in a solution of 2 % PFA and 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences, Hatfield, PA) for 3 h ...
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bioRxiv - Bioengineering 2020Quote: ... bone pieces were washed with double distilled water for 2 minutes and embedded in Epon epoxy resin dissolved in acetone (Embed 812, EMS, USA). The Epon embedding was carried out in 5 steps ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... atala eye was immersed for prefixation in 2.5% Glutaraldehyde/2% paraformaldehyde in 0.1M Sodium Cacodylate buffer (pH 7.4) (Electron Microscopy Sciences, PA, USA) for 2h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Copper grids containing thin sections were post-stained for 20 min in 2% aqueous uranyl acetate (Electron Microscopy Sciences; Cat #22400) and for 5 min in Reynolds lead citrate (Reynolds ...
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bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were transfer into slicksealTM microcentrifuge tube (National Scientific, CA170S-BP) and fixed with 2% paraformaldehyde (Electron Microscopy Science, Nm15710) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of sample was applied to glow-discharged (30 s) Quantifoil copper grids (R2/2, 200 mesh, Electron Microscopy Sciences), blotted on both sides for 4 s ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 ul chromatin samples with a concentration of about 0.2 mg/ml DNA were applied to Quantifoil R2/2 200 mesh copper grids (EMS Q250-CR2). Vitrification was conducted by plunging into liquid ethane using our FEI Vitrobot Mk IV Grid Plunging System at 100% humidity ...
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bioRxiv - Cancer Biology 2022Quote: ... sample coverslips were washed 2 times in staining buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
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bioRxiv - Neuroscience 2022Quote: ... P21 and P28) were deeply anesthetized and perfused with 0.9% saline followed by 2% paraformaldehyde (PFA) and 2.5% glutaraldehyde (EMS, Hatfield, PA, USA) in 0.1 M phosphate buffer (PB) ...
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bioRxiv - Systems Biology 2023Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Cell Biology 2023Quote: ... The block edges were covered with additional strips of carbon tape and 2-4 strips of copper tape (EMS; cat#77802) and coated with a layer of colloidal silver liquid (EMS ...
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bioRxiv - Molecular Biology 2023Quote: HEK-293 cells (60%-70% of confluency) were fixed by 2.5% glutaraldehyde and 2% paraformaldehyde (both from Electron Microscopy Science, Inc.) solution for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The blocks corresponding to the area of interest (∼0.8 mm from the epicenter) were trimmed and post-fixed in 2% osmium tetroxide (Electron Microscopy Sciences). The tissues were then dehydrated in graded ethanol and embedded in Embed 812 (EMS) ...
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bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Bioengineering 2023Quote: ... 20 uL of BEV sample was fixed in 2% EM-grade paraformaldehyde (157-4-100; Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... they were fixed for 90 min at room temperature with 4% PFA/2% Sucrose solution supplemented with 0.1% Glutaraldehyde (GA, Electron Microscopy Sciences), washed five times with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM EDTA was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were washed with cold PBS supplemented with 0.1 mM CaCl2 and 2 mM MgCl2 (PBSCM) and fixed in 4% paraformaldehyde (Electron Microscopy Sciences)/4% sucrose for 8 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the slides were mounted using MS Shield Mounting Medium With 4,6- diamidino-2-phenylindole (DAPI) and DABCO™ (EMS #17989-20).
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bioRxiv - Cancer Biology 2023Quote: ... MFPs were fixed in cold 2% v/v glutaraldehyde with 4% paraformaldehyde in 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences) for 4 hr followed by one wash in the same buffer ...
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bioRxiv - Cell Biology 2024Quote: ... sample slides were washed 2 times in Staining Buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
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bioRxiv - Cell Biology 2024Quote: ... or 30 min and fixed by adding an equal volume of 4 % pre-warmed paraformaldehyde (Electron Microscopy Sciences, final concentration 2 %), followed by 20 min incubation at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... sample slides were washed 2 times in Staining Buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) storage buffer ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low- barium PBS for 5 min ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed with acetone and infiltrated with increasing concentrations (25, 50, 75 and 100%; 2 hours each) of EPON resin (Electron Microscopy Sciences). 100% EPON solution was exchanged two times in 12-hour steps ...
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bioRxiv - Biophysics 2022Quote: ... They were then kept at room temperature for ∼2 h before being applied to carbon-coated 200-hex copper grids (EMS, Hatfield PA). Freshly ionized grids were floated on a 10 ul drop of sample for 1 minute ...
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bioRxiv - Microbiology 2022Quote: ... grids were washed by contact with 2 drops of milliQ water and were negatively stained with 20 µl of 1% uranyl acetate (Electron Microscopy Sciences) for 1 min ...