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Citations for Electron Microscopy Sciences :
551 - 600 of 729 citations for Ataxin 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Specimen holders were opened under liquid nitrogen and transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS) and 0.1% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed in distilled water by centrifugation and resuspension and then post-fixed in 1% aqueous osmium tetroxide (Electron Microscopy Sciences) for 30 minutes at RT ...
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bioRxiv - Microbiology 2021Quote: ... Three μL of cell culture were applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Neuroscience 2020Quote: ... The EV sample (5μl) was adsorbed for 1 min to a carbon-coated mesh grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-s exposure to a glow discharge (25 mA) ...
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bioRxiv - Neuroscience 2021Quote: Adult animals were perfused with 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA), brains ...
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bioRxiv - Biochemistry 2021Quote: ... and 3 µL of sample was applied onto glow-discharged C-flat grids (EMS CF-1.2/1.3-4Au-1. 50) for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: After 8 washes with 0.1 M cacodylate buffer at pH 7.4 (SigmaAldrich) the tissue was post fixed by 1% osmium tetroxide (Electron Microscopy Sciences) and 0.6% K3Fe(CN)6 in a 0.1 M cacodylate buffer for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... ∼5 × 109 infected RBCs (iRBCs)] and schizont stage (40–46 hpi, ∼1.5 × 109 iRBCs) were harvested and crosslinked with paraformaldehyde (1% final concentration; EMS, USA) at 37°C for 15 min with agitation and then immediately neutralized by adding glycine (0.125 M final concentration ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed in water and then maleate buffer (MilliporeSigma) (pH=6.0) and stained in 0.05 M Maleate Buffer containing 1% uranyl acetate (Electron Microscopy Sciences) at 4°C overnight ...
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bioRxiv - Biophysics 2022Quote: ... A 3.5 μL sample containing the complex (1 mg/ml) was applied to a glow-discharged UltraAuFoil grid (R1.2/1.3, mesh 300) (Electron Microscopy Sciences), and then blotted and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2019Quote: ... with a layer of ultra-thin carbon (Agar Scientific) were glow-discharged at 45 mA for 1 min using a K100X Glow Discharge Unit (EMS). A 4 µl-droplet was then applied directly onto the carbon-side of the grid loaded into the humidity chamber of a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: Three microliters of the Ric-8A:ΔN31Gαi1:4Nb complex at 0.4 mg/ml with 0.01% NP40 were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (Electron Microscopy Sciences). Grids were blotted for 4 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... Samples were treated for 1 h with 1% aqueous uranyl acetate and were then dehydrated in a graded ethanol series and embedded in EMBed-812 resin (EMS). After 48 h of polymerization at 60 °C ...
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bioRxiv - Microbiology 2019Quote: Negative stain specimens were prepared by applying 4 µL of protein solution (0.1 mg/mL + 1 mM AMP-PnP) onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS). After 30 s the protein solution was blotted with a piece of filter paper ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were allowed to dry in open air for 1 hour prior to coverslipping with Cytoseal (Electron Microscopy Sciences #18007).
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bioRxiv - Biochemistry 2019Quote: ... 5µl of the EV sample was adsorbed for 1 min to a carbon-coated mesh grid (# CF400-CU EMS www.emsdiasum.com) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Organoids were fixed in 3% glutaraldehyde and 1% paraformaldehyde in 0.08 M sodium acodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Embryo buffer E3 was removed and replaced with 1 mL SHIELD Perfusion Solution with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences), shaking overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... for one hour and then washed three times for 15 min each with the same buffer before secondary fixation in 1% osmium tetroxide (EMS) prepared in Sorensen’s Phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... after which 5-μL were deposited in a glow-discharged Quantifoil grid (200-mesh copper R2/1, Electron Microscopy Sciences), followed by manual blotting and plunge freezing in a 50/50 ethane propane mix (Airgas ...
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bioRxiv - Microbiology 2020Quote: ... The mixture (2.5 μL) was applied to freshly glow-discharged (30 s) Quantifoil R 3.5/1 grids (Electron Microscopy Sciences) and manually blotted before being flash-frozen in liquid ethane ...
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bioRxiv - Physiology 2022Quote: ... they were rinsed 3 times 5 minutes in PB buffer and then postfixed by a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were rinsed 3 × 5 min in PB and post-fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% potassium ferrocyanide in PB buffer for 2 h at RT ...
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bioRxiv - Genetics 2023Quote: AG437 pezo-1(R2405P) early L4 hermaphrodites were washed three times in M9 and soaked in 48 mM Ethyl methane sulfonate (EMS) solution for four hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... pelleted and washed with PBS before being resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences 30525-89-4) + 0.0075% glutaraldehyde (Sigma G6257 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then incubated in 0.1 M sodium cacodylate buffer supplied with 1% OsO4 and 0.8% potassium ferricyanide (Electron Microscopy Sciences), for 2 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
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bioRxiv - Neuroscience 2023Quote: ... iAssembloids were first fixed with 4% paraformaldehyde (16% stock, diluted 1:4 in DPBS, Electron Microscopy Sciences, Cat. No. 15710) and then incubated in a 25% formamide (MilliporeSigma ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed in water and stained for 1 h at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), and then washed again in water and dehydrated in a graded series of ethanol ...
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bioRxiv - Bioengineering 2023Quote: Purified DNA origami structures (1–10 nM) were adsorbed onto freshly glow discharged copper grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 4 minutes ...
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bioRxiv - Genetics 2023Quote: The two epididymides of mature males were recovered in 1 mL of 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) and the sperm were allowed to swim for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... pieces of tissue were rinsed with 0.1M sodium cacodylate buffer (cacodylate buffer) three times and then postfixed on ice with reduced osmium containing 1% osmium tetroxide (EMS) and 1% ferrocyanide (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Formvar carbon film on 200-mesh copper grids (FCF200-Cu) and uranyl formate (16984-59-1) from Electron Microscopy Sciences were used for sample preparation for negative-stain transmission electron microscopy (TEM).
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bioRxiv - Biochemistry 2023Quote: ... transcardial perfusion was performed using 1× phosphate-buffered saline (PBS) and 4% paraformaldehyde (PFA; Electron Microscopy Sciences, the United States). The mouse liver and kidney were dissected and postfixed in 4% PFA for 6 hours at 4°C ...
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bioRxiv - Physiology 2024Quote: Isolated TALs and DCTs were embedded into 1% agarose and fixed in 3% formaldehyde/glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... and cover-slipped with ThermoFisher Gold Seal Cover Glass 24 x 60mm No.1 thickness (Electron Microscopy Sciences, 63770-01). Sections were imaged using a Leica CTR6000/B microscope to quantify sulfatide accumulation in different brain areas.
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bioRxiv - Neuroscience 2024Quote: ... Subsequently, sections were incubated in DAPI (1:1000, 10 mins) and mounted with Fluoromount G (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Biochemistry 2024Quote: The specimen was vitrified using 3 μL of freshly purified BasC-Nb71 complex in 0.17% DM applied to glow-discharged Quantifoil R 0.6/1 Cu 300 mesh grids (Electron Microscopy Sciences), at a concentration of 2.2 mg/ml ...
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bioRxiv - Biophysics 2024Quote: ... 2.5 µl of sample was applied to 300 mesh Quantifoil R 2/1 copper grids (Electron Microscopy Sciences, Hatfield PA) that had been previously glow discharged (15 mA ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The slices were then transferred to capped glass vials and postfixed with 1% osmium tetroxide diluted in PBS from a 4% aqueous solution (EMS 19170) for 30 minutes on a rotator ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3 μl of the particle dispersion was applied on 400-mesh C-Flat CF-2/1-4C copper grids (Protochips, USA) negatively glow discharged with a Plasma Cleaner (EMS, USA) at 50 mA for 45 s ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...