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Citations for Electron Microscopy Sciences :
451 - 500 of 700 citations for Acyl protein thioesterase 2 LYPLA2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The slurry was fixed in 0.2 µm filter-sterilized 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in 1x phosphate buffered saline solution (PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... before being post-fixed with osmium-ferricyanide (2 % OsO4, Electron Microscopy Sciences, Cat. # 19100; 1% K3Fe(III)(CN)6 ...
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bioRxiv - Neuroscience 2024Quote: ... 2018) then cut into 2-3mm wide cubes which were pierced using a puncher (0.75mm, 57395, EMS) to insert the larva ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then fixed for 10 min at room temperature with 2% PFA (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... the eyes were immersed in a petri dish with fixative solution containing 2% paraformaldehyde (EMS, Cat# 15700) + 2.5% glutaraldehyde (EMS ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Biophysics 2021Quote: The nanodisc incorporated protein was individually applied to freshly glow-discharged Quantifoil R1.2/1.3 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 4 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Cell Biology 2020Quote: ... Grids with the collected monolayers facing up were stained at once with 2% uranyl formate (Electron Microscopy Sciences) in water by depositing 4 μL on the grid and simultaneously blotting the excess solution ...
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bioRxiv - Neuroscience 2019Quote: Cultured and non-cultured ganglia were placed in 2% paraformaldehyde solution (Electron Microscopy Sciences, 8% stock, Cat. #1578100) for 4 hours and then sucrose-protected at 4 °C in 30% sucrose for 72 hours ...
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bioRxiv - Biophysics 2020Quote: ... and permeabilized for 2 min with 0.3 % glutaraldehyde (GA) + 0.25 % Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37° C ...
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bioRxiv - Neuroscience 2021Quote: ... followed by fixation with 2% PFA (w/v; prepared from a 20% stock solution, Electron Microscopy Sciences: 15713) in cell culture medium for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... following which they were placed in a mixture (500 μL) of 2% OsO4 (Electron Microscopy Sciences, cat#19150) and 0.1 M sodium cacodylate buffer for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were then postfixed for 1 h on ice with 2 mL of 1% osmium tetroxide (EMS #RT19134) and 1% potassium ferrocyanide (Sigma-Aldrich #P3289 ...
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bioRxiv - Biophysics 2020Quote: ... and permeabilized for 2 min with 0.3 % glutaraldehyde (GA) + 0.25 % Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37° C ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µL of particle solution (20 mg/mL) was applied on a CF400-CU grid (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were fixed in 2% PFA/2.5% glutaraldehyde/0.1 M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Lateral ventricle walls were fixed for 18 h at 4 °C othen subdissected into anterior ...
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bioRxiv - Neuroscience 2020Quote: ... unbuffered OsO4 aqueous solution for 2 hours and lead aspartate solution (0.03 M, pH 5.0 adjusted by KOH, EMS) at 50 °C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were intracardially perfused with NaCl 0.9% followed by PFA 2% (Electron Microscopy Sciences 15713, diluted in PBS). Brains were post-fixed in PFA 2% and cryoprotected in 20% sucrose overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were stained by a 2 minute bath in 0.1% cresyl violet acetate (Electron Microscopy Sciences #26089-01), followed by a 2 minute rinse in distilled water ...
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bioRxiv - Microbiology 2020Quote: ... and stained with 2% uranyl formate (pH 5.5– 6.0) on thin carbon-layered 400 mesh copper grids (EMS)46 ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with pentobarbital and perfused with 2% paraformaldehyde (PFA, Paraformaldehyde Granular, Electron Microscopy Sciences, Hatfield, PA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... (2) fixed with 1.5% paraformaldehyde and 1.5% glutaraldehyde in 0.1M sodium cacodylate (Electron Microscopy Sciences, Hatfield, PA, USA), pH 7.3 for 1 h at room temperature followed by 3 washes with 0.1M sodium cacodylate ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; 17985-50; Electron Microscopy Sciences, Hatfield, PA, USA). Images were taken with an All-in-One Fluorescence Microscope BZ-X710 (KEYENCE Corp. ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Physiology 2023Quote: ... Ovaries used for MIMS were cut in half and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) and embedded in LR white (EMS) ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos prepared for immunostaining were fixed overnight at 4°C in 2% PFA (Electron Microscopy Sciences, Catalog #15710). Embryos were either embedded in 4% low-melt agarose/PBS + 0.1% Tween and cut into 200um sections using a Leica VT1200 or stained as intact whole-mounts ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3 times in 1X PHEM buffer and post-fixed in 2% OsO4 (Electron Microscopy Sciences) +1,5% potassium ferrocyanide in water 1h in the dark ...
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bioRxiv - Biophysics 2024Quote: 3 μL of purified MscS between 0.1 and 0.7 mg/mL was applied to a glow discharged Quantifoil R 1.2/1.3 + 2 nm C 400 Cu mesh grid (EMS), a holey grid with a thin 2 nm continuous carbon film ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Cell Biology 2021Quote: Exocyst holocomplex was mixed with liposomes composed of 95 mol% POPC and 5 mol% PI(4,5)P2 and protein A coated 5 nm gold fiducials (Electron Microscopy Sciences) for 3 minutes and to a final protein concentration of 1 μM ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in gels were transferred to nitrocellulose membranes and subsequently immobilized by 0.1% glutaraldehyde (15 min) (Electron Microscopy Sciences; Hatfield, PA). After blocking in Tris-Buffered Saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... Cryo-EM grids were prepared by applying 3.5 μl of protein to the glow-discharged Quantifoil R1.2/1.3 200-copper mesh grids from Electron Microscopy Science (Q2100-CR1.3). The grids were blotted for 3 s ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Developmental Biology 2021Quote: ... whole-mount preparations of the lateral wall of lateral ventricles of four animals per group were dissected and fixed with 2 % PFA + 2.5 % glutaraldehyde (EMS) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Pathology 2022Quote: ... perfused in toto (PBS; 10mL) and fixed (2% formaldehyde EMS #15710, 2.5% glutaraldehyde EMS #16220 in 0.1M Phosphate Buffer). Kidneys were harvested and fixed in the same solution ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with CyFACS and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS (Rockland) ...
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bioRxiv - Cancer Biology 2019Quote: Fresh pancreas samples were cut into 1 mm fragments and immersion fixed in a modified Karnovsky’s fixative (51) (2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS) ...
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bioRxiv - Biophysics 2021Quote: ... and permeabilized for 2 min with 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and Thermo Fisher, 28314) in pre-warmed (37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and rinsed in distilled water prior to en bloc staining with 2% uranyl acetate (22400, Electron Microscopy Sciences, USA). After dehydration through a graded ethanol series ...
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bioRxiv - Neuroscience 2021Quote: One 5 mm sample from each naïve nerve was fixed for 48 hours at 4° C in a solution of 2% glutaraldehyde (16216, EMS), 3% paraformalydehyde (15754-S ...
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bioRxiv - Biochemistry 2021Quote: ... Ycf1 sample was applied to a CFlat-1.2/1.3-4C-T (WT Ycf1) or QF-1.2/1.3-4Au grid (E1435Q Ycf1) purchased from Electron Microscopy Sciences. Grids were placed inside of a Leica EM GP2 equilibrated to 10°C and 80% humidity ...