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Citations for Electron Microscopy Sciences :
201 - 250 of 2892 citations for 7 nitro 3 oxido 6 4 phenylpiperazin 1 yl 2 1 3 benzoxadiazol 3 ium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biochemistry 2020Quote: Freshly prepared filament samples (3 μL) were applied to glow-discharged Lacey carbon film grids (LC200-Cu; Electron Microscopy Sciences, Hatfield PA). The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biophysics 2020Quote: ... the head was placed onto the scanner directly after cleaving the glued-on 3 mm Muscovite Mica disk (Electron Microscopy Sciences, Hatfield). The concentrated sample was diluted in imaging buffer to 60 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Plant Biology 2021Quote: ... zmcd1-1 and zmcd1-2 (EMS mutants), as well as the F1 hybrids (obtained by crossing the EMS mutants with test inbred lines ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: Flow chambers were created using tape placed ~3 mm apart on a glass slide to adhere coverslips coated with 0.1% collodion in amyl acetate (Electron Microscopy Sciences, Hatfield, PA). For standard gliding assays with β-cardiac myosin eGFP ...
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bioRxiv - Cell Biology 2023Quote: ... astrocytes loaded with either unlabeled or AF647 NHS ester-labeled tau fibrils (0N4R PFF, described earlier in methods) were fixed with 3% (vol/vol) paraformaldehyde (Electron Microscopy Sciences, 15714) and 0.1% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... 3) three double-sided 9 x 0.12 mm round well electron microscopy grade double sided adhesive spacers (Electron Microscopy Sciences, Cat# 70327-8S), 4 ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and coverslips were mounted over slides in Fluoro-Gel (EMS; 17985-10) and imaged by spinning disc confocal microscopy and Aperio Versa 8 fluorescent slide scanner.
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Plant Biology 2021Quote: Marchantia thalli were cut in small pieces (1×2 mm) and fixed immediately in buffered phosphate 0,1 M paraformaldehyde 4% (Electron Microscopy Sciences), glutaraldehyde 2,5% (TAAB Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
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bioRxiv - Biophysics 2019Quote: ... and then fixed for 15 minutes at room temperature (RT) with pre-warmed (at 37 °C) 3% paraformaldehyde (PFA, 15710, Electron Microscopy Sciences, Hatfield, PA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Genetics 2023Quote: ... 5-10 μl of 1% PFA solution pH 9.2 containing 0.15% Triton X-100 and 3 mM DTT was placed in each well of a 12-well glass slide (Electron Microscopy Sciences, 63425-05) and one ZP-free oocyte was transferred to each well ...
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bioRxiv - Developmental Biology 2023Quote: The abt4-1 (ttg2-6) mutant was isolated from an ethyl methanesulfonate (EMS)-mutagenesis mutant population in gl2-3 mutant background (Salk_039825 ...
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bioRxiv - Cell Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (Electron Microscopy Sciences, #17985-10) and viewed by confocal microscope (Leica SP8 ...
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bioRxiv - Cell Biology 2024Quote: ... were immersed in and fixed by 4 % glutaraldehyde (48 h/4 °C), post-fixed by 1% osmium tetroxide (2 h, RT) and were embedded into Epon resin (Electron Microscopy Sciences) as described previously32 ...
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bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl sample was applied to copper 400-mesh grids with an ultra-thin layer of carbon (Electron Microscopy Sciences, cat. No. CF400-Cu-UL), that had been glow-discharged in air for 30 seconds ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Neuroscience 2022Quote: ... or 7 days prior to intracardiac perfusion with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 15714) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... A portion of each EV sample (10 μl) was fixed in a 1:1 solution using 4% EM-grade paraformaldehyde (Electron Microscopy Sciences, 157-4-100) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were fixed in 4% paraformaldehyde (a 1:1 mixture of 8% paraformaldehyde, Electron Microscopy Sciences (EMS) 157-8 ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were fixed in 4% paraformaldehyde (a 1:1 mixture of 8% paraformaldehyde, Electron Microscopy Sciences (EMS) 157-8 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in a 1:1 solution of 4% aqueous osmium tetroxide (Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in a 1:1 solution of 4% aqueous osmium tetroxide (Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Cell Biology 2023Quote: ... and then post-fixed for 1 hr at 4°C in 1% osmium tetroxide (Electron Microscopy Sciences) in 0.1 M Sodium Cacodylate ...
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bioRxiv - Cell Biology 2019Quote: ... 1:2 propylene oxide to Eponate12 resin formula (EMS). Samples were embedded in resin molds and placed in an oven set at 60°C overnight for polymerization.
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bioRxiv - Immunology 2022Quote: ... Quantifoil R 2/1 holey carbon copper grid (EMS) were used for sample vitrification ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were further fixed for 7 days in 2% glutaraldehyde (Electron Microscopy Sciences: 16320)/ 4% paraformaldehyde/ 0.1M sodium-cacodylate at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
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bioRxiv - Cell Biology 2023Quote: ... 6-diamidino-2-phenylin dole (DAPI) (17985-50, Electron Microscopy Sciences). Cells visualized and photographed by fluorescence microscopy (Nikon ...
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bioRxiv - Molecular Biology 2023Quote: Carbon-coated holey grids Quantifoil R 0.6/1 Cu 300 and CF-2/1-3C (EMS) were used for preparing cryo-EM samples for apo GSEC1B and GSEC1B-Aβ46 complex ...
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bioRxiv - Cell Biology 2021Quote: ... neurons were fixed on DIV 1 with a 4% PFA solution (1-part 16% PFA-Electron Microscopy Sciences, 1-part Milli-Q H2O ...
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bioRxiv - Immunology 2023Quote: ... Cell post-fixation was as follows: 1 hour at 4°C with 1% osmium tetroxide (Electron Microscopy Sciences) and 0,8% potassium ferricyanide in PBS ...
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bioRxiv - Biophysics 2022Quote: ... The void peaks from each purification were frozen similarly at the eluted concentration after dilution 1:1 with 6 nm BSA-tracer gold (Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibodies were removed using washing buffer (0.1% BSA in 0.1 M PB) and grids were incubated with 6 nm gold-conjugated donkey-anti-rabbit IgG (1:25, #25104, Electron Microscopy Sciences) for 20 min.
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bioRxiv - Neuroscience 2022Quote: ... at a dose of 50 mg/kg either 4 hours or 7 days prior to intracardiac perfusion with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 15714) in PBS.
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bioRxiv - Neuroscience 2020Quote: ... 1% paraformaldehyde with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA). The rest of the animal was placed in a −40°C freezer ...
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bioRxiv - Neuroscience 2021Quote: ... then samples were embedded for 2 h with 1:1 acetone and epon resin (Embed-812, EMS), followed by 100% epon resin at 60°C for 2 days ...
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bioRxiv - Cell Biology 2021Quote: ... fixed for 1 h in 4% PFA (Electron Microscopy Sciences, Cat# 15714)/Hydra medium ...
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bioRxiv - Immunology 2022Quote: ... The sample was stained by 6 µL 2% uranyl acetate solution (Electron Microscopy Sciences, Catalog number 22400-2) twice ...