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Citations for Electron Microscopy Sciences :
151 - 200 of 2731 citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: Cells were fixed with 1% (for Piezo1) or 4 % (for phalloidin) paraformaldehyde (PFA, Electron Microscopy Sciences 15713-S) for 10 minutes and washed two times with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 2h at room temperature and stored in 1% PFA in PBS at 4°C and finally fixed with 2.5% GA and 0.05% malachite green (EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Temporal bones were immediately removed and fixed via perfusion of 1 ml cold 4% PFA (Electron Microscopy Science) in 1X PBS through the oval and round windows ...
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bioRxiv - Microbiology 2023Quote: ... The anterior half of the cranium was harvested as described[72] and placed into 4% paraformaldehyde (Cat. 157-8-100, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Genetics 2023Quote: Testis and epididymides samples from 8-16-week-old mice were fixed for 24 h in PBS/4% PFA (Electron Microscopy Sciences), dehydrated in a graded ethanol series ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Systems Biology 2022Quote: ... 6F- Volume was completed to 8 ml with room temperature 1 X PBS and then 500 μl of 16 % PFA solution (Electron Microscopy Sciences, cat. # 15710) was added ...
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bioRxiv - Plant Biology 2023Quote: ... drd1-6 (EMS)55 ...
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bioRxiv - Biochemistry 2021Quote: ... and 3 µL of sample was applied onto glow-discharged C-flat grids (EMS CF-1.2/1.3-4Au-1. 50) for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems) ...
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bioRxiv - Cell Biology 2021Quote: Organoids were fixed in 3% glutaraldehyde and 1% paraformaldehyde in 0.08 M sodium acodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Physiology 2024Quote: Isolated TALs and DCTs were embedded into 1% agarose and fixed in 3% formaldehyde/glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours ...
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bioRxiv - Cell Biology 2019Quote: ... organoids were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3× with PBS and fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, PA, USA) in PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times with PBS and then incubated with a 4% PFA solution (Electron microscopy sciences) for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Microbiology 2022Quote: Monolayers of HFF infected with Toxoplasma from 6 or 24 h were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, PA) in 0.25 M HEPES (ph7.4 ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
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bioRxiv - Immunology 2020Quote: Pyroptotic BMDMs were fixed for 1 hour at room temperature (RT) in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 15710), then incubated with PBS containing 50 mM NH4Cl (Sigma ...
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bioRxiv - Physiology 2019Quote: ... hPCLS were washed with PBS (3X 1ml) and fixed in 1 ml of 4% PFA (EMS EM grade#5710) overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences, cat. #15714S, diluted in PBS) and incubated at 4°C for 15 minutes with rocking ...
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bioRxiv - Cell Biology 2024Quote: 1 × 106 cells in HBS were fixed using 4% paraformaldehyde (PFA) (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 3 mL of cells was harvested by centrifugation for 3 min at 1500 × g at room temperature and fixed for 30 min in 1 mL of 2.5% (v/v) glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) in 0.1 M cacodylate buffer (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... high parasitemia culture was pelleted by centrifugation (1.5 g, 3min, RT) and resuspended in freshly prepared PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) and 0.0075% gluteraldehyde (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then infiltrated for 1 h with 1:1 PO:Embed 812 (EMS), and then overnight in 1:2 PO:Embed 812 ...
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bioRxiv - Cell Biology 2022Quote: ... freshly prepared without accelerator (at a ratio of 22.6 g EMbed812 : 16.1 g DDSA : 9.8 g NMA, all from Electron Microscopy Sciences), followed by 1 h with 67% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Cancer Biology 2021Quote: Human or mouse FFPE tumor tissue sections were deparaffinized in xylene alternative (Histo-Clear, EMS; 3×5 min) and rehydrated in EtOH/H2O gradient series (100% ...
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bioRxiv - Biochemistry 2023Quote: 3 μl of dAK (1 mg/ml) was applied to QUANTIFOIL Cu R300 1.2/1.3 (Electron Microscopy Sciences, catalog no. Q3100CR1.3) that had been glow discharged using a PELCO easiGlow device (Ted Pella Inc. ...
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bioRxiv - Developmental Biology 2020Quote: Organoid cultures were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5-mm clean coverglass (EMS # 72200-31 ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS, 69039-60). The silicon chamber was mounted on a 1.5-mm clean cover glass (EMS ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and coverslips were mounted over slides in Fluoro-Gel (EMS; 17985-10) and imaged by spinning disc confocal microscopy and Aperio Versa 8 fluorescent slide scanner.
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bioRxiv - Cell Biology 2019Quote: ... samples of 1-2 mm thickness were fixed through immersion in 5% (w/v) glutaraldehyde (Biological Grade, EMS, #16500) in 0.05M phosphate buffer pH 7.3 for 24 hours ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was immersed for 5 min in reduced osmium solution containing 1% osmium tetroxide (OsO4; Electron Microscopy Sciences) and 1.5% potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells in FACS buffer (PBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences) for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The grid was quickly washed using a 4-μL drop of milli-Q (MilliporeSigma) water and stained with 4 μL of negative stain solution composed of either 1% (w/v) uranyl acetate (EMS) with 0.5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: Adult animals were perfused with 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA), brains ...
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bioRxiv - Neuroscience 2021Quote: ... Embryo buffer E3 was removed and replaced with 1 mL SHIELD Perfusion Solution with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences), shaking overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... transcardial perfusion was performed using 1× phosphate-buffered saline (PBS) and 4% paraformaldehyde (PFA; Electron Microscopy Sciences, the United States). The mouse liver and kidney were dissected and postfixed in 4% PFA for 6 hours at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The grid was then rinsed with deionized water and a 5 μL 4% uranyl acetate replacement (EMS) droplet was placed on to the grid ...
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bioRxiv - Immunology 2023Quote: FACS-sorted naïve CD4+ T cells from LNs and spleen were pelleted at 400 g for 4 mins by swinging bucket Eppendorf 5810 into BEEM conical capsule tips (EMS, #69913-05), and fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (CB ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were stained en bloc with 0.25% uranyl acetate then dehydrated in graded series of ethanol on ice followed by one wash with 100% ethanol and two washes with propylene oxide (5 min each) and embedded with EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Sections were cut at 60 nm on a Leica EM UC6 ultramicrotome ...
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bioRxiv - Biochemistry 2023Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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bioRxiv - Microbiology 2023Quote: ... 3 μl of this mixture was blotted on a glow-discharged Quantifoil R 2/1 Copper 300 mesh grid (Electron Microscopy Sciences) and plunge-frozen in liquid ethane using a Vitrobot plunge freezer (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and placed in 1:1 100% ethanol: Hardener 1 Technovit 7100 (Electron Microscopy Sciences 14653) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Fixative was removed by washing 5 x 3 min in 0.1 M cacodylate buffer and samples were incubated in 2% osmium (OsO4, EMS) in 0.1 M cacodylate buffer for 30 min at RT ...