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Citations for Electron Microscopy Sciences :
51 - 100 of 103 citations for 6 Methylheptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with freshly prepared and filtered 1% tannic acid (TA, CAS#1401-55-4, EMS) in water using a Pelco BioWave microwave for seven 1-minute cycles alternating between 150 W and 0 W power ...
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bioRxiv - Cell Biology 2019Quote: ... 8.5 µm, 6 µm, 6.5 µm, 6.5 µm, 6.5 µm for P0, AB, P1, ABa, ABp and EMS, respectively ...
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bioRxiv - Biochemistry 2023Quote: ... and then the grids were blotted for 5-6 seconds using VitrobotTM Filter paper (Electron Microscopy Sciences) with a blot force of 6 ...
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bioRxiv - Molecular Biology 2023Quote: ... heat-indued antigen retrieval was performed for 30 min in Buffer A (Electron Microscopy Sciences, pH 6). Tissue sections were blocked using 0.3% H2O2 ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then freeze substituted at - 90°C with 0.1% (w/v) uranyl acetate and 0.01% (w/v) tannic acid (EMS) in acetone for 6h using a Leica EM AFS2 with a processor Leica EM FSP ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected brains were moved through 6 wells (12ηl/well) containing a fixation solution (4% paraformaldehyde, Electron microscopy sciences #15713 in PBT (0.3% Triton in PBSX1 ...
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bioRxiv - Biophysics 2022Quote: ... 6-8 μl of sample were applied on a freshly glow-discharged grid (Electron Microscopy Sciences, Hatfield, PA) and incubated for 5-10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were then washed twice with PBS and fixed with 6% paraformaldehyde (PFA, Electron Microscopy Sciences: 15710) and 0.2% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... The sample was stained by 6 µL 2% uranyl acetate solution (Electron Microscopy Sciences, Catalog number 22400-2) twice ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; 17985-50; Electron Microscopy Sciences, Hatfield, PA, USA). Images were taken with an All-in-One Fluorescence Microscope BZ-X710 (KEYENCE Corp. ...
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bioRxiv - Neuroscience 2023Quote: Third instar larvae and pupae brains at 6 hours after puparium formation (APF) were dissected in ice-cold 0.1M cacodylate buffer at pH 7.4 (EMS). The buffer was immediately replaced with a solution containing 2% glutaraldehyde ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated in cacodylate buffer containing 1% low molecular weight tannic acid for 30 min (Electron Microscopy Sciences) between the osmication step and the uranyl acetate step ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mg/mL tannic acid and 0.5 mg/mL saponin in HKB) for 40 min on a thermanox plastic coverslip (EMS, USA), followed by washing in HKB buffer and then water ...
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bioRxiv - Microbiology 2020Quote: ... and stained at room temperature for 1 hour with 1% (wt/vol) low-molecular weight tannic acid (Electron Microscopy Science) in 01 ...
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bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
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bioRxiv - Molecular Biology 2020Quote: ... we spread 5×104 protoplasts onto a regeneration plate and subjected them to a treatment of either 6 sec of 15 W UV or 12 μg/ml ethyl methanesulfonate (EMS) to cause random mutations in the genomes ...
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bioRxiv - Immunology 2022Quote: ... The sample was double-stained with 6 µl of 2% (w/v) uranyl formate (Electron Microscopy Sciences, catalog number 22450) for 5 seconds (first stain ...
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bioRxiv - Genomics 2019Quote: ... cells were washed 3×3 minute in PBS and fixed for 30 minutes in 6% paraformaldehyde (Electron Microscopy Sciences 15710) in 1x PBS ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
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bioRxiv - Neuroscience 2024Quote: 6 μL of sample was applied to a glow discharged carbon coated electron microscopy grid (CF150-Cu, Electron Microscopy Sciences) for 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... The roots of 7 to 10 day old seedlings were collected and fixed for 30 min with 2-(N-morpholino) ethane sulfonic acid (MES) buffer solution pH 5.7 containing 4% paraformaldehyde (32% methanol-free solution; Electron microscopy Sciences). pH was adjusted to 5.7 with KOH solution.
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 500 μM nucleic acid intercalator iridium (191Ir and 193Ir, Fluidigm) in PBS with 1.6% PFA (Electron Microscopy Sciences) for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Microbiology 2022Quote: Monolayers of HFF infected with Toxoplasma from 6 or 24 h were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, PA) in 0.25 M HEPES (ph7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
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bioRxiv - Microbiology 2023Quote: ... RAW264.7 and THP-1 cells were thawed and seeded as mentioned earlier on 6 well plates containing #1.5H glass cover slips (Electron Microscopy Sciences). Cells were grown and maintained as described above ...
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bioRxiv - Immunology 2023Quote: ... sections prepared from mouse lungs frozen in OCT were stained with Sirius Red (0.1% Sirius Red in saturated picric acid, Electron Microscopy Sciences, 26357-02) for 60 min followed by a 2-min 0.01 N HCl wash ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
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bioRxiv - Biophysics 2022Quote: ... The void peaks from each purification were frozen similarly at the eluted concentration after dilution 1:1 with 6 nm BSA-tracer gold (Electron Microscopy Sciences).
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bioRxiv - Microbiology 2021Quote: ... GUVs were placed on a 6 mm diameter chamber made from a silicon sheet using a core sampling tool (EMS #69039-60) and imaged prior to protein addition to ensure proper GUV formation and suspension ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5-mm clean coverglass (EMS # 72200-31 ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 M glucose) and placed on 6 mm diameter chambers made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5 mm clean coverglass (EMS # 72200-31 ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS, 69039-60). The silicon chamber was mounted on a 1.5-mm clean cover glass (EMS ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and coverslips were mounted over slides in Fluoro-Gel (EMS; 17985-10) and imaged by spinning disc confocal microscopy and Aperio Versa 8 fluorescent slide scanner.
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bioRxiv - Microbiology 2022Quote: Purified eCIS samples were briefly centrifuged to remove impurities and 5 μL of supernatant was applied onto freshly glow discharged carbon-coated copper grids (CF400-CU, 6 nm, 400 mesh, Electron Microscopy Sciences). Samples were allowed to adsorb for 2 minutes before being blotted off by filter paper and grids were washed twice with filtered distilled water ...
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibodies were removed using washing buffer (0.1% BSA in 0.1 M PB) and grids were incubated with 6 nm gold-conjugated donkey-anti-rabbit IgG (1:25, #25104, Electron Microscopy Sciences) for 20 min.
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bioRxiv - Plant Biology 2022Quote: ... from −30 °C to 0° in 6 h) This was followed by washing in acetone and then infiltration in Spurr resin (EMS, Hatfield, PA, US) at graded concentration and temperature (30% for 10 h from 0 °C to 20°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... 6-8 μl of 1 nM DNA origami sample was wicked onto a glow-discharge-cleaned copper grid (Electron Microscopy Sciences, Hatfield, PA) and incubated for 5-10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...
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bioRxiv - Cell Biology 2023Quote: Wandering third-instar larvae were dissected in ice cold Ca2+-free saline and fixed for 6 minutes in Bouin’s Fixative (Electron Microscopy Sciences, Cat# 15990). Dissections were washed several times with 1X PBS and then permeabilized with 1X PBS containing 0.1% Triton-X (Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: Solutions of extruded vesicles were mixed with 6 nm colloidal gold fiducial markers (Aurion, Wageningen, Netherlands) and applied to glow-discharged C-flat holey carbon grids (Electron Microscopy Science, Hatfield, PA) or QUANTIFOIL R 2/2 holey carbon grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Cell Biology 2019Quote: ... Cells were allowed to grow for 6 h and then compressed for 18 h before being fixed with 4% paraformaldehyde (# 30525-89-4, Electron Microscopy Sciences, Hatfield, PA). The non-invasive cells on the upper chamber were removed with cotton swabs ...
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bioRxiv - Neuroscience 2022Quote: ... The mammary gland was excised and fixed overnight from either lactating wild-type (C57BL/6) or oxytocin knockout mice in 4% PFA (Electron Microscopy Sciences, Hatfield, PA). Tissue was incubated at 37 °C in CUBIC Reagent 1 A (10 wt% Triton ...