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Citations for Electron Microscopy Sciences :
701 - 750 of 873 citations for 6 Hydroxy 3 nitro 2 picoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Eyes were fixed in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed and then fixed in FACS buffer supplemented with 2% PFA (15710-S, Electron Microscopy Sciences) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Etched coverslips were cleaned of oil and fixed in a 2% solution of glutaraldehyde in PBS (Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2022Quote: ... before being fixed by perfusion using 0.15M cacodylate buffer (pH 7.4) containing 2% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences) as well as 2mM CaCl2 at 35°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... four to five serial sections were picked up per 2 × 1 mm formvar and carbon coated slot grid (EMS). Because the Aclar coverslip ...
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bioRxiv - Cell Biology 2022Quote: ... placed on 200 mesh copper TEM grids and were stained with 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA) and Reynold’s lead citrate (Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat. no. 19100 Electron Microscopy Sciences) for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were washed with cold flow cytometry buffer and fixed with 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). The samples were stored at 4°C wrapped in foil up to 1 week prior to flow cytometric analysis ...
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were then stained with a 2% (w/v) solution of uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). High-resolution TEM images were captured using a JEOL JEM-2010 TEM and JEOL JEM-2100 Field Emission Gun TEM ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was then stained by adding 10 µL of 2% filtered uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Grids were then blotted dry with filter paper and stored at RT and visualized by using a bottom-mounted Eagle 4k x 4k camera on a Tecnai F20 TEM (FEI Company ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... cells were rinsed with PBS containing calcium and magnesium and fixed using 2% paraformaldehyde (PFA) (Electron Microscopy Sciences # 15710) in PBS for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... high Grid-50 Glass Bottom is a 35 mm then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS Cat# 16000) and 4% pFormaldehyde (EMS Cat# 15700 ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were stained for 25 seconds with a 2% aqueous solution of uranyl acetate from Electron Microscopy Sciences. Following staining ...
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bioRxiv - Biophysics 2024Quote: ... followed by embedding in 2% agarose and post-fixed in 0.1 M osmium tetroxide (SKU 19152, Electron Microscopy Science). Dehydration was achieved through a series of acetone solutions with increasing concentrations ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
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bioRxiv - Developmental Biology 2022Quote: Wild type (WT) embryos and WT embryos injected with lama1 morpholino were fixed in 2% formaldehyde (Electron Microscopy Science #15710), 2.5% glutaraldehyde (EMS #16220 ...
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bioRxiv - Neuroscience 2019Quote: ... 18 larva of the two different ages were fixed whole in Karnovsky’s fixative (2.5% glutaraldehyde and 2% paraformaldehyde solution diluted in 0.1 M cacodylate buffer at pH 7.4; EMS RT15720; Karnovsky, 1965) for at least 24 hours at 15-20 °C and at a pH of 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... Three μL of cell culture were applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Immunology 2022Quote: ... a 2 µl aliquot of EV preparation was placed onto a carbon film grid (Electron Microscopy Sciences, Hatfield, PA, USA) for 1 minute ...
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bioRxiv - Synthetic Biology 2020Quote: ... was adsorbed onto formvar-carbon coated copper grids for 2 min and negatively stained with uranyl acetate replacement stain (UAR-EMS) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
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bioRxiv - Bioengineering 2019Quote: ... agarose RoCs with ROs and RPE were fixed in the chambers with Karnovsky buffer (2.5% glutaraldehyde, 2% paraformaldehyde, 0.1 M sodium cacodylate buffer, pH 7.4) (Electron Microscopy Sciences, Germany) for 12 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The medial shell of the NAc and a piece of tissue spanning the entire VTA were excised and prepared for EM by staining sequentially with 2% osmium tetroxide (EMS) in cacodylate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was quickly extracted and cut into 2 mm coronal slices using a rodent brain matrix (Electron Microscopy Sciences). Slices were placed on ice cold PBS and PFC and VCX were further dissected and collected for RNA extraction ...
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bioRxiv - Biophysics 2020Quote: ... 270 µL of GPMV solution was pipetted from the bottom of the Eppendorf tube and sandwiched between 2 coverslips coated with 0.1% BSA and separated by a 0.5 mm thick silicone isolator (Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2022Quote: ... EVs were fixed by positioning the grid on top of a 20 µL drop of 2% paraformaldehyde (EMS, 157-4) for 10 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
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bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde in 0.1M Sodium Cacodylate buffer (cat. no. 15960-01 Electron Microscopy Sciences) for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat ...
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bioRxiv - Microbiology 2023Quote: Immunofluorescence assay was performed on PHA-E-immobilised parasites by fixation with 2% paraformaldehyde and 0.008% glutaraldehyde (Electron Microscopy Sciences) and permeabilisation with 0.1% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed with glutaraldehyde 2.5% in 0.1 M sodium cacodylate buffer supplemented with 2 mM of calcium chloride (Electron Microscopy Sciences), post-fixed with 1% OsO4 and 1.5% potassium ferrocyanide in sodium cacodylate buffer ...
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bioRxiv - Neuroscience 2023Quote: ... We applied a standard reduced osmium thiocarbohydrazide osmium (rOTO) en bloc staining protocol (Kislinger et al., 2020a) including postfixation in 2% osmium tetroxide (EMS), 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... the cells on nanostructures were washed with PBS and fixed in 0.1 M sodium cacodylate buffer (pH 7.2) supplemented with 2% glutaraldehyde (Electron Microscopy Sciences) and 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed in water and stained for 1 h at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), and then washed again in water and dehydrated in a graded series of ethanol ...
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bioRxiv - Plant Biology 2023Quote: ... and the resulting cell pellets were fixed using a mixture of 2% [v/v] paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) and 2% [v/v] glutaraldehyde (Agar Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... They were subsequently transcardially perfused with 15 ml of 0.1M sodium phosphate buffer pH 7.4 and then 60 ml of 2% paraformaldehyde (15710-S, Electron Microscopy Sciences) 1% glutaraldehyde (G7526 ...
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bioRxiv - Microbiology 2023Quote: Genomic libraries were constructed and sequenced by MiSeq using paired-end sequencing (2 × 300 bp) for 17 strains generated by EMS mutagenesis and one strain ...
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bioRxiv - Genetics 2023Quote: ... the supernatant was discarded and the pellet resuspended in primary fixating buffer (2% glutaraldehyde/0.1 M sodium cacodylate buffer, pH 7.4, Electron Microscopy Sciences) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed in 0.1 M cacodylate buffer (pH 7.4) with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Biochemistry 2023Quote: Prom1 and Ttyh1 EVs were vitrified on 300-mesh gold Quantifoil R 1.2/1.3 + 2 nm Carbon grids (Electron Microscopy Sciences). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... 2.5 µl of sample was applied to 300 mesh Quantifoil R 2/1 copper grids (Electron Microscopy Sciences, Hatfield PA) that had been previously glow discharged (15 mA ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed by immersion in Modified Karnovsky’s Fixative (2% Paraformaldehyde and 2.5% Glutaraldehyde in 0.1 M Sodium Cacodylate Buffer, pH 7.4 (Electron Microscopy Sciences (Hatfield, PA), #15960-01) ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were then mounted with DAPI (diamidino-2-phenylindole)-free Fluoro-Gel on glass slides (Electron Microscopy Sciences, USA). Cells were scanned using a 63x oil immersion objective mounted on a confocal microscope (LSM 710 Meta ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Molecular Biology 2021Quote: BF PolIE RNAi cells were grown with or without doxycycline for 24 hours then incubated with 2 mM of ethylmethanesulfonate (EMS; Sigma) or DMSO (vehicle ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3 μl of the particle dispersion was applied on 400-mesh C-Flat CF-2/1-4C copper grids (Protochips, USA) negatively glow discharged with a Plasma Cleaner (EMS, USA) at 50 mA for 45 s ...
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bioRxiv - Genomics 2019Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...