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Citations for Electron Microscopy Sciences :
1 - 50 of 2565 citations for 4 5 5 5 Tetrafluoro 4 trifluoromethoxy pentan 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and 5 mL of 4% Paraformaldehyde (PFA) (EMS) diluted in RNase free PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... the frozen sections were postfixed 5 min with 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in PBS at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed after 5 days of differentiation with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS and immunofluorescence staining was performed with FoxA2 (1:50 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Shi/Shi:Rag2-/- mice grafted with control and RRMS hiOLs (n=4 per group) were perfused with 1% PBS followed by a mixture of 4% paraformaldehyde/5% glutaraldehyde (Electron Microscopy Science) in 1% PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Plant Biology 2020Quote: Approximately 5 000 seeds (0.1 g) of kor1-4 JGP were mutagenized with ethyl methanesulfonate (EMS, Sigma) as described (6) ...
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bioRxiv - Biophysics 2021Quote: ... The grid was then rinsed with deionized water and a 5 μL 4% uranyl acetate replacement (EMS) droplet was placed on to the grid ...
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bioRxiv - Genomics 2022Quote: ... 5% fixation buffer [5% paraformaldehyde (EMS, cat. no. 50-980-493), 1.25x dPBS] was added to each well ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with ddH2O three times 5 min and incubated in 1% thiocarbohydrazide (EMS, cat# 2231-57-4) diluted in ddH2O for 20 min ...
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bioRxiv - Bioengineering 2024Quote: ... before being fixed in pre-warmed (5 min in 37° C water bath) 4% formaldehyde (Electron Microscopy Sciences, 15710) in PBS solution for 5 min and permeabilized in pre-warmed (to 37° C ...
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bioRxiv - Neuroscience 2024Quote: ... after drying (5 min, at 37°C in a non-humid oven) and gentle fixation with ice-cold 4% Paraformaldehyde (Electron Microscopy Sciences, #15700; 15 min, at 4°C). Nonspecific binding was blocked by incubating the sections in 5% normal donkey serum in Tris-buffered saline (1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... cells were first washed with PBS for 5 minutes and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, PA, USA) in PBS containing 0.1% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of parasite culture at 5% parasitemia was washed with PBS and re-suspended in a fresh fixative solution (4% Paraformaldehyde (EMS) and 0.0075% glutaraldehyde (EMS ...
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bioRxiv - Genomics 2024Quote: Liver tissue sections (3-5 mm thickness) were fixed by submerging in fresh 4% v/v paraformaldehyde (PFA; EMS 15710) in 1X Phosphate Buffered Saline (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... heated in Volvic mineral water 5 min at 70°C to extend the tardigrade body and directly fixed with 4% formalin (15686, EMS) in 1X PBS-1% Triton-X100 (PBS-Tx ...
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bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were washed twice for 5 min in wash buffer and fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium (Ionpath ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of this sample at 2 µg Chl mL−1 was applied to a glow discharged (60 s, 25 mA) 400 mesh Cu grid with 5-6 nm formvar with 3-4 nm carbon (Electron Microscopy Sciences). The sample was negatively stained with 2% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then gently washed with phosphate buffer saline (PBS) at room temperature for 5 minutes and then fixed with 4% paraformaldehyde (PFA) (cat:15710-S, Electron Microscopy Science) for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... cells grown on 13-mm round poly-L-lysine-coated coverslips were fixed by adding to the culture medium an equal volume of 4% PFA (Electron Microcopy Sciences) and 5% glutaraldehyde (GTA) (Electron Microscopy Sciences) in 0.2 M cacodylate pH 7.4 for 15 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... cleared in xylene (2×5 minutes) and embedded in Entellan (EMS, Hatfield, PA).
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bioRxiv - Developmental Biology 2023Quote: ... Grids were stained with 5 μl of 2% uranyl acetate (Electron Microscopy Sciences) for 45 seconds ...
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bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Cell Biology 2022Quote: ... fine-tipped forceps (Dumont #5, EMS). Holding the coverslip vertically ...
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bioRxiv - Cell Biology 2021Quote: ... and left incubating in 5 mL 2% uranyl acetate (Electron Microscopy Sciences, Catalog #22400) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 20% methyl-5-norbornene-2,3-dicarboxylic anhydride and 2% catalyst dimethylbenzylamine (Electron Microscopy Sciences) over four days ...
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bioRxiv - Neuroscience 2024Quote: ... One 4 mm cover glass (round, #1.5 thickness, Electron Microscopy Sciences) was adhered to the bottom of a custom titanium insert and placed onto the brain to gently compress the underlying cortex and dampen biological motion during imaging ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
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bioRxiv - Microbiology 2024Quote: ... subtilis colonies were inoculated into 5 mL of LB medium supplemented with 5 µL of ethyl methanesulfonate (EMS), and cultured at 37°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... Dumont tweezers (Style 5, Electron Microscopy Sciences) were used ...
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bioRxiv - Bioengineering 2022Quote: ... 5% normal donkey serum (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 mm (Electron Microscopy Sciences, 7229605) glass coverslips were prepared by plasma etching with oxygen for 1 minute (GLOW Plasma System ...
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bioRxiv - Neuroscience 2024Quote: ... with one 4 mm cover glass (round, #1.5 thickness, Electron Microscopy Sciences) adhered to the bottom ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples were immersion-fixed for at least one overnight at 4°C in 2.5% glutaraldehyde + 4% paraformaldehyde (Electron Microscopy Science) in a 1mM MgCl2 ...
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bioRxiv - Pathology 2024Quote: ... 4% PFA was supplemented with 2% glutaraldehyde (EMS). For biochemical analyses such as immunoblotting ...
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bioRxiv - Immunology 2024Quote: ... Slides were fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium ...
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Immune organization in sentinel lymph nodes of melanoma patients is prognostic of distant metastasesbioRxiv - Cancer Biology 2024Quote: ... Slides were fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium ...
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bioRxiv - Genetics 2023Quote: ... one half was post-fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) in PBS for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
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bioRxiv - Cell Biology 2021Quote: Exocyst holocomplex was mixed with liposomes composed of 95 mol% POPC and 5 mol% PI(4,5)P2 and protein A coated 5 nm gold fiducials (Electron Microscopy Sciences) for 3 minutes and to a final protein concentration of 1 μM ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...