Labshake search
Citations for Electron Microscopy Sciences :
251 - 300 of 1476 citations for 1 2 Bis Pentabromophenyl Ethane Unlabeled 25 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... were immersed in and fixed by 4 % glutaraldehyde (48 h/4 °C), post-fixed by 1% osmium tetroxide (2 h, RT) and were embedded into Epon resin (Electron Microscopy Sciences) as described previously32 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and labeled for 1 h with 20 µg/mL anti-PEG10 antibody diluted in EM blocking solution (EMS, Aurion). Samples were washed in PBS for 15 min ...
-
bioRxiv - Physiology 2019Quote: ... hPCLS were washed with PBS (3X 1ml) and fixed in 1 ml of 4% PFA (EMS EM grade#5710) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cells were then resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences, cat. #15714S, diluted in PBS) and incubated at 4°C for 15 minutes with rocking ...
-
bioRxiv - Physiology 2023Quote: ... One microliter of this resuspension is added to 1 mL of Mammalian Ringer’s Solution (Electron Microscopy Sciences #11763-10) containing isolated myofibers from the flexor digitorum brevis muscle (FDB ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 µL of unroofing buffer and 2 µL of 10 nm BSA gold tracer solution (Electron Microscopy Sciences) was added to the grid prior to placing in a Leica plunge freezer ...
-
bioRxiv - Immunology 2022Quote: ... The sample was stained by 6 µL 2% uranyl acetate solution (Electron Microscopy Sciences, Catalog number 22400-2) twice ...
-
bioRxiv - Biophysics 2023Quote: ... 3 μL of the sample was applied to glow-discharged (45 s) Quantifoil (2/2; Electron Microscopy Sciences) grids ...
-
bioRxiv - Cell Biology 2021Quote: ... in PHEM (MT immunostaining) or PBS (all other immunostaining) before mounting to slides using Citifluor (17970-25; Electron Microscopy Sciences). Coverslips were sealed to slides using clear nail polish.
-
bioRxiv - Cancer Biology 2023Quote: ... Coverslips were mounted on microscope slides using the Fluoromount G mounting medium (ref 17984-25, EMS Diasum, Hatfield, Pennsylvania, USA). Nikon ECLIPSE NI-E microscope with a 100X immersion objective (Nikon ...
-
bioRxiv - Neuroscience 2023Quote: ... the resin-infiltrated sections were flat-embedded onto fluoropolymer films (ACLAR®, Pennsylvania, USA, Electron Microscopy Sciences, cat# 50425-25) covered with resin and kept at 55 °C in a convection oven for 3 days ...
-
bioRxiv - Plant Biology 2024Quote: ... washed five times with PBS and mounted onto slides with the antifading agent CutiFluorTM AF1 (Electron Microscopy Sciences 17970-25). FITC and DAPI fluorescence were then immediately imaged ...
-
bioRxiv - Cell Biology 2019Quote: ... resuspended in freshly prepared 2% KMnO4 (EMS) and incubated for 3 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Quantifoil R2/2 (Electron Microscopy Sciences, #Q2100CR2) were treated with chloroform overnight and then glow discharged (30 seconds at 25 mAh) ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by fixative containing 2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS ...
-
bioRxiv - Immunology 2020Quote: ... 2% uranyl formate (UF; Electron Microscopy Sciences) was neutralized with 25 mM NaOH final concentration ...
-
bioRxiv - Neuroscience 2019Quote: ... and 2% PFA (Electron Microscopy Sciences, #15710) in 0.1 M sodium cacodylate buffer (Sigma ...
-
bioRxiv - Neuroscience 2021Quote: ... and 2 % glutaraldehyde (Electron Microscopy Sciences 16220) in PBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... fixed with 2% paraformaldehyde (Electron Microscopy Sciences) and stained with anti-Brachyury PE-conjugated Antibody (R&D ...
-
bioRxiv - Microbiology 2022Quote: ... Holey carbon Quantifoil (R2×2) grids (EMS) were glow discharged in air using a Quorum technology instrument for 60 seconds at atmospheric pressure of 0.1 prior to application of the sample ...
-
bioRxiv - Cell Biology 2023Quote: ... 2% sodium citrate diluted (EMS, Cat# 21140) in water ...
-
bioRxiv - Microbiology 2023Quote: ... 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA), and 0.15% alcian blue (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... 2% Osmium tetroxide (Electron Microscopy Sciences, 19134) and 5% H2O in acetone (PanReac AppliChem ...
-
bioRxiv - Microbiology 2023Quote: ... fixed with 2% paraformaldehyde (Electron Microscopy Sciences)/ 0.5% glutaraldehyde (Sigma-Aldrich ...
-
bioRxiv - Biophysics 2022Quote: ... A 3.5 μL sample containing the complex (1 mg/ml) was applied to a glow-discharged UltraAuFoil grid (R1.2/1.3, mesh 300) (Electron Microscopy Sciences), and then blotted and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Biochemistry 2020Quote: Three microliters of the Ric-8A:ΔN31Gαi1:4Nb complex at 0.4 mg/ml with 0.01% NP40 were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (Electron Microscopy Sciences). Grids were blotted for 4 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: Negative stain specimens were prepared by applying 4 µL of protein solution (0.1 mg/mL + 1 mM AMP-PnP) onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS). After 30 s the protein solution was blotted with a piece of filter paper ...
-
bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... Embryo buffer E3 was removed and replaced with 1 mL SHIELD Perfusion Solution with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences), shaking overnight at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... pelleted and washed with PBS before being resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences 30525-89-4) + 0.0075% glutaraldehyde (Sigma G6257 ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
-
bioRxiv - Genetics 2023Quote: The two epididymides of mature males were recovered in 1 mL of 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) and the sperm were allowed to swim for 15 min at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: Cells were pre-fixed with 2% paraformaldehyde/2.5% glutaraldehyde and post-fixed in 2% Osmium tetroxide (OsO4; EMS, USA). After block staining with 1.5% potassium ferrocyanide (sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
-
bioRxiv - Neuroscience 2021Quote: ... the grid was stained one time for 3 minutes and three times for 1-minute each using 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Excess stain was wicked away in between the steps ...
-
bioRxiv - Cell Biology 2021Quote: ... were applied to carbon-coated and glow discharged (2 minutes at 7x10-1 mbar) 300-mesh copper EM grids (Electron Microscopy Sciences; CF300-CU) and incubated for four minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... thick resin sections (250 nm) were cut and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were decorated with fiducial marker and stained with Hoechst to visualize nuclear regions ...
-
bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
-
bioRxiv - Microbiology 2020Quote: ... purified EVs were resuspended in 30 μL D-MEM (0% FCS) and mixed with a low volume of Glutaraldehyde 25% (final concentration = 2.5%) (16220, Electron Microscopy Sciences). After 10 min of fixation at RT ...
-
bioRxiv - Immunology 2020Quote: ... The mitotic cells were collected by mitotic shake-off and then seeded onto round 25 mm cover glasses (Electron Microscopy Sciences) and placed onto the cell-holding chamber of an LSM510-Meta confocal microscope equipped with a CO2 incubator and a heating chamber ...
-
bioRxiv - Biochemistry 2022Quote: ... was carried out on the various samples with 200-mesh gold grids coated with extra thick (25-50 nm) formvar-carbon film (EMS, USA) made hydrophilic by glow discharging at 5 mA for 60 s ...
-
bioRxiv - Neuroscience 2023Quote: ... Labeled sections were mounted onto glass slides and coverslipped with fluorescent mounting medium (Citofluor AF1; Cat#17970-25, Electron Microscopy Sciences).
-
bioRxiv - Biochemistry 2023Quote: ... Negative stain TEM was carried out on the various samples with 200-mesh gold grids coated with extra thick (25-50 nm) formvar-carbon film (EMS, USA) made hydrophilic by glow discharging at 5 mA for 60 s ...
-
bioRxiv - Microbiology 2019Quote: ... wild-type 81116 strain cell culture grown to OD600 = 5 was applied onto glow-discharged C-flat holey carbon films 2/2 200 mesh (EMS). A Leica EM GP (Leica ...
-
bioRxiv - Cell Biology 2022Quote: ... The tissues were cut into small pieces of about 2 mm2 and then fixed by 0.1 M phosphate buffer (pH 7.4) including 2% PFA (Electron Microscopy Sciences) and 2% glutaraldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 μl of 10 μM GMPCPP-MT (polymerised as described above) were applied to a glow-discharged C-flat 2/2-4C grid (EMS) at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were then placed in two serial washes of PBS solution with gentle agitation for 30 seconds each prior to being submerged for 2 hours in 2% osmium solution (RT 19172, EMS). Thereafter ...
-
bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
-
bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...