Labshake search
Citations for Electron Microscopy Sciences :
401 - 445 of 445 citations for Fmoc Rink Amide OctaGel Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Samples were transitioned from ethanol to infiltration with propylene oxide and embedded in Embed-812 hard resin (Electron Microscopy Sciences, Hatfield, PA). Blocks were sectioned at 50-60 ηm and stained with uranyl acetate and Reynolds’ lead citrate ...
-
bioRxiv - Cell Biology 2022Quote: ... The samples were dehydrated in graded concentrations of ethanol (30%, 50%, 70%, 90%, 95%, and 100%) and embedded in Epon resin (Electron Microscopy Sciences). Ultrathin sections (80 nm ...
-
bioRxiv - Cell Biology 2021Quote: ... A graded series of ethanol was used for dehydration with acetone as a transition solvent and infiltration in Hard Plus resin (Electron Microscopy Sciences). Samples were cured for 48 hours at 60 degrees C and serial sections were cut at 50nm using a Diatome 45-degree ultra-diamond knife or an AT-4 35-degree diamond knife on a Leica UC7 ultramicrotome ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were transitioned from ethanol to infiltration with propylene oxide and embedded in Embed-812 hard resin (Electron Microscopy Sciences, Hatfield, PA). Blocks were sectioned at 50-60 ηm and stained with uranyl acetate and Reynolds’ lead citrate ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then dehydrated in acetone and incubated in a series of three progressively concentrated acetone-Epon 812 resin (EMS, Cat#RT14120) mixtures ...
-
bioRxiv - Pathology 2020Quote: ... The tissues were subsequently serially dehydrated in ethanol and propylene oxide and embedded in EMBed 812 resin (Electron Microscopy Sciences, Hatfield, PA). Thin sections ...
-
bioRxiv - Microbiology 2021Quote: ... samples were dehydrated through a graded ethanol series (20%, 40%, 60%, 80%, 90%, 100%, 100%, 100%) followed by infiltration with an Embed-812 epoxy resin (Electron Microscopy Sciences):acetone series of 1:3 for 2 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... Two rounds of 100% acetonitrile (30 minutes each) served as a transitional solvent step before proceeding to epoxy resin (EMS Hard Plus). A progressive resin infiltration series (1:2 resin:acetonitrile (e.g ...
-
bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
-
bioRxiv - Neuroscience 2023Quote: ... At −50 °C samples were washed four times in ethanol for 30 minutes each before gradual infiltration with HM20 resin (Electron Microscopy Sciences). Polymerization of the resin was done with a UV light source and was carried out at −50 °C for 48 hours followed by post-polymerization at room temperature for 2 days.
-
bioRxiv - Plant Biology 2023Quote: Samples of Arabidopsis tissues were dehydrated in a successive ethanol series (20%, 40%, 60%, 80%, 95%, and 100%) and embedded in LR White resin (Electron Microscopy Sciences ). Thin sections (1 µm ...
-
bioRxiv - Neuroscience 2023Quote: ... They were carefully placed on a fine layer of resin between 2 sheets of ACLAR® embedding films (EMS, cat# 50425-25) for polymerization at 55 °C for 72 h ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were transitioned from ethanol to infiltration with propylene oxide and embedded in Embed-812 hard resin (Electron Microscopy Sciences, Hatfield, PA). Blocks were sectioned at 50 to 60 ριm and stained with uranyl acetate and Reynolds’ lead citrate ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were transitioned from ethanol to infiltration with propylene oxide and embedded in Embed-812 hard resin (Electron Microscopy Sciences, Hatfield, PA). Blocks were sectioned at 50-60ριm and stained with uranyl acetate and Reynolds’ lead citrate ...
-
bioRxiv - Neuroscience 2024Quote: ... Two rounds of 100% acetonitrile (30 minutes each) served as a transitional solvent step before proceeding to epoxy resin (EMS Hard Plus). A progressive resin infiltration series (1:2 resin:acetonitrile (e.g ...
-
bioRxiv - Neuroscience 2024Quote: ... dehydrated using increasing concentration of ethanol and finally with propylene oxide and embedded in epoxy resin (Electron Microscopy Science, Hatfield, PA, USA). Thin sections (70 nm ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... We then mixed the embedding resin following the instructions on our kit (EMbed 812 kit, Electron Microscopy Sciences, Fort Washington, PA, USA) for a hard resin (20ml EMBed-812 ...
-
bioRxiv - Neuroscience 2023Quote: ... Two rounds of 100% acetonitrile (30 minutes each) served as a transitional solvent step before proceeding to epoxy resin (EMS Hard Plus). A progressive resin infiltration series (1:2 resin:acetonitrile (e.g ...
-
bioRxiv - Cell Biology 2022Quote: ... a ∼1.0 mm3 piece was trimmed of any excess resin and mounted to an 8 mm aluminum stub using silver epoxy Epo-Tek (EMS, Hatfield, PA). The mounted sample was then carefully trimmed into a smaller ∼0.5 mm3 tower using a Diatome diamond trimming tool (EMS ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were dehydrated in ethanol and transferred through several washes with acetone to be finally embedded in Durcupan resin (Electron Microscopy Sciences). Imaging was done using a serial block face scanning electron microscope by Zeiss with a step-size of 0.1µm ...
-
bioRxiv - Microbiology 2022Quote: ... the cells were dehydrated in increasing concentrations of ethanol (50%-100%) at room temperature and embedded in EPON resin (Electron Microscopy Sciences). Ultrathin sections (70-80 nm ...
-
bioRxiv - Bioengineering 2023Quote: ... tibia-implant samples were subjected to an ethanol dehydration series (70%, 80%, 90%, 95%, 95%, 100%, 100%) in 48 hr intervals and gradually infiltrated with Embed812 resin (Electron Microscopy Sciences) in sequential ratios of 1:3 ...
-
bioRxiv - Molecular Biology 2024Quote: Fragments of cuticles with scales were dehydrated through a graded ethanol series (70–100%) followed by infiltration in Embed812 epoxy resin (Electron Microscopy Sciences) for 5 days ...
-
bioRxiv - Molecular Biology 2024Quote: ... sections were prepared from cured resin blocks on a Leica Microtome Ultracut UCT and mounted on carbon film–coated 200 mesh copper TEM grids (Electron Microscopy Sciences) and imaged at the Imaging and Analysis Center ...
-
bioRxiv - Microbiology 2024Quote: ... The fixed EVs were dehydrated through a graded series of ethanol and embedded in Embed 812 resin (Electron Microscopy Sciences, Hatfield, PA). Thin sections were stained with uranyl acetate and lead citrate and then imaged with a Zeiss EM902 electron microscope ...
-
bioRxiv - Cancer Biology 2024Quote: ... a ∼1.0 mm3 piece was trimmed of excess resin and mounted on an 8 mm aluminum stub using silver epoxy Epo-Tek (EMS, Hatfield, PA). The mounted sample was then carefully trimmed into a smaller ∼0.5 mm3 tower using a Diatome diamond trimming tool (EMS ...
-
bioRxiv - Microbiology 2020Quote: ... Then cells were put in propylene oxide for 1 h and infiltrated overnight in a 11 mixture of propylene oxide and Spurr’s low viscosity resin (Electron Microscopy sciences, Hatfield, PA). The cells were embedded in Spurr’s resin and polymerized at 60°C for 48 h ...
-
bioRxiv - Cell Biology 2020Quote: ... The samples were dehydrated in graded acetone series and embedded for 72 hours at 60°C in Embed-812 resin (EMS, Hatfield, PA, USA). Ultrathin sections were collected on copper grids ...
-
bioRxiv - Neuroscience 2022Quote: ... The cell pellets were then infiltrated with a 50:50 mixture of acetone and embedding resin (Embed 812, Electron Microscopy Sciences, Hatfield, PA) for 72 hours ...
-
bioRxiv - Developmental Biology 2023Quote: ... dehydrated in graded series of ethanol followed by dehydration with propylene oxide and infiltration with Embed 812 resin (Electron Microscopy Sciences, Hatfield, PA). Tissue polymerized in Embed 812 resin at 60°C ...
-
bioRxiv - Plant Biology 2023Quote: ... The samples were then washed three times in acetone and infiltrated with an ascending series of Spurr’s low-viscosity embedding resin (Electron Microscopy Sciences, Hatfield, PA) (25 ...
-
bioRxiv - Plant Biology 2022Quote: ... from −30 °C to 0° in 6 h) This was followed by washing in acetone and then infiltration in Spurr resin (EMS, Hatfield, PA, US) at graded concentration and temperature (30% for 10 h from 0 °C to 20°C ...
-
bioRxiv - Bioengineering 2024Quote: ... dehydrated in a graded series of ethanol ranging from 30% to absolute solution and embedded in resin (EMBed-812, Electron Microscopy Sciences, USA). 80 nm thick sections from at least four blocks from Ctr iPSC-derived MYOrganoids in presence or absence of Ctr fibroblasts were stained with uranyl acetate and lead citrate ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by post-fixation in 1% osmium tetroxide in 0.1 M cacodylate buffer and embedding in Embed 812 Resin (Electron Microscopy Sciences, Hatfield, PA). One-µm wide sections were sliced on a Leica RM2265 rotary microtome equipped with a diamond knife ...
-
bioRxiv - Neuroscience 2022Quote: ... specimens were dehydrated using serially graded ethanol solutions (30%, 50%, 70%, 90% and 100%) and infiltrated and embedded in Spurs resin (Electron Microscopy Sciences, Hatfield, PA) following the manufacturer’s recommendations ...
-
bioRxiv - Developmental Biology 2024Quote: ... tissue was then dehydrated by incubation in a graded ethanol series followed by incubation in 1:1 ethanol:Spurr resin (Electron Microscopy Sciences; Hatfield, PA, USA) overnight ...
-
bioRxiv - Microbiology 2022Quote: ... 0.5 cm sections of the colon were resected as a cylinder prior to splaying open and embedded with a methacrylate resin (Technovit 8100, Electron Microscopy Sciences, Hatfield, PA). The remaining colon sections were cut longitudinally and fixed on absorbent paper with Carnoy solution ...
-
bioRxiv - Neuroscience 2022Quote: ... The latest dehydration steps performed with 100% ETOH and propylene oxide were followed by infiltration with epoxy resin (EMBed-812; Electron Microscopy Science, PA USA). Cochleae were cut with a Leica EM UC7 ultramicrotome ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were fixed in 2.5% glutaraldehyde plus 1% paraformaldehyde in 0.1 M cacodylic buffer for 30 min and then embedded in resin using an Embed 812 kit (Electron Microscopy Sciences, Hatfield, PA, USA) and sectioned at 80 nm with a diamond knife (Diatome ...
-
bioRxiv - Plant Biology 2020Quote: ... 100% of cold EtOH) prior to being resin infiltrated and embedded using the Lowicryl Monostep Embedding Media HM20 (#14345, Electron Microscopy Sciences, Hatfield, PA, USA) using either heat or UV polymerization ...
-
bioRxiv - Microbiology 2020Quote: ... and held at that temperature for an additional 12 h before warming to room temperature and infiltrating into Epon-Araldite resin (Electron Microscopy Sciences, Port Washington PA). Samples were flat-embedded between two Teflon-coated glass microscope slides and the resin polymerized at 60 °C for 48 h ...
-
bioRxiv - Neuroscience 2021Quote: ... with a final dehydration in propylene oxide for 5 min at room temperature prior to resin infiltration with a 1:1 mixture of propylene oxide and EPON (Embed-812, 14121, Electron Microscopy Sciences, Hatfield, PA, USA) for 1 h and two fresh replacements of 100 % EPON (first for 1 h ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were then infiltrated in a 1:1 mixture of 90% ethanol and LR White resin with accelerator (Electron Microscopy Sciences, Hatfield, PA, USA) for 16 h at 4 °C on a shaker ...
-
bioRxiv - Neuroscience 2024Quote: ... Ducupan Resin A/M, cat. no. 44611; Sigma-Aldrich, B hardener, cat. no. 44612; Sigma-Aldrich, D hardener cat. no. 44614; Electron Microscopy Sciences, DMP 30 cat. no. 13600)