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Citations for Electron Microscopy Sciences :
401 - 450 of 1241 citations for 3 Ethyl 3 methyloxolane 2 5 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Cell Biology 2023Quote: ... astrocytes loaded with either unlabeled or AF647 NHS ester-labeled tau fibrils (0N4R PFF, described earlier in methods) were fixed with 3% (vol/vol) paraformaldehyde (Electron Microscopy Sciences, 15714) and 0.1% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... 3) three double-sided 9 x 0.12 mm round well electron microscopy grade double sided adhesive spacers (Electron Microscopy Sciences, Cat# 70327-8S), 4 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... anesthetized larvae were moved to a rectangular plate (4.5 cm x 3 cm x 1 cm) coated with Sylgard (Electron Microscopy Sciences, Hatfield, PA). After removing excess water ...
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bioRxiv - Evolutionary Biology 2024Quote: Negative staining grids were prepared using a standard protocol,86 pipetting 3-8 µL samples on glow discharged ultrathin carbon film supported copper grids (EMS CF400-Cu-UL), followed by staining with 2% (w/v ...
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bioRxiv - Physiology 2024Quote: ... Hearts were perfused for 3 min with PBS at 37°C to wash out the blood followed by a fixative solution of Glutaraldehyde 3% in 0.1M Cacodylate buffer pH7.4 (Electron Microscopy Sciences-cat#16538-15) for less than 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
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bioRxiv - Neuroscience 2022Quote: ... were injected intraperitoneally in n=3 control and n=3 KA Nestin-GFP animals 30 minutes before perfusion with a PFA 4 %-Glutaraldehyde 0.5 % (Cat#16536-05, Electron Microscopy Sciences, Hatfield, PA, USA) mixture ...
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bioRxiv - Developmental Biology 2023Quote: ... After rinsing in 0.1 M PBS at room temperature (10 min x 3) and post-fixed in a sequence of aqueous 1% OSO4 (#19150, Electron Microscopy Science, Hatfield, PA) in 0.1 M PBS ...
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bioRxiv - Cell Biology 2024Quote: ... coli was washed 3 times in PBS and then immediately used live in phagocytic assays or fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA) in PBS and stored for a few weeks.
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 20,000 seeds homozygous for the SCRpro::GFP-SCR transgene were treated overnight at room temperature with 10 mL 0.1% ethyl methanesulfonate (EMS, Sigma M0880, MO, USA). After thorough washing ...
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bioRxiv - Genomics 2020Quote: Ten million cells from two MSC preparations at early (P2 and 3) and late (P7 and 9) passages were cross-linked with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 10 min. ...
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bioRxiv - Cell Biology 2021Quote: Immediately after cold exposure mice were anaesthetized with isoflurane (3%) and perfused with heparinized saline followed by 4% paraformaldefyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA). Fat pads were carefully dissected and postfixed overnight in 4% PFA at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl sample was applied to copper 400-mesh grids with an ultra-thin layer of carbon (Electron Microscopy Sciences, cat. No. CF400-Cu-UL), that had been glow-discharged in air for 30 seconds ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low- barium PBS for 5 min ...
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bioRxiv - Systems Biology 2023Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 min ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Genomics 2022Quote: ... 5% fixation buffer [5% paraformaldehyde (EMS, cat. no. 50-980-493), 1.25x dPBS] was added to each well ...
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bioRxiv - Biochemistry 2024Quote: CryoEM grids were prepared by applying 3.5 μL of 0.6 mg/mL NX11Q21.5-DNA48 in TBS with 2.5% glycerol to glow-discharged 400 mesh C-flat grids (CF-2/2-4C, 2 μm holes, 2 μm spacing; Electron Microscopy Sciences). The grids were blotted with a blot force of 0 for 6.5 seconds at 100% humidity and 20 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated 2 min on 2% paraformaldehyde (Electron Microscopy Sciences), then incubated for 30 sec on 2% uranyl acetate (aqueous ...
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bioRxiv - Biophysics 2022Quote: ... CF-2/2-4C (CF-224C-100) (Electron Microscopy Sciences) cryoEM grids ...
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bioRxiv - Neuroscience 2021Quote: ... 2% paraformaldehyde (EMS) in 0.1 M sodium cacodylate buffer (EMS) ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS), 2.2mM CaCl2 diluted in 50 mM MOPS buffer (pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: Quanifoil R 2/2 200 mesh copper grids (EMS #Q2100-CR2) were glow discharged at 25mA for 25s using a Pelco easiGlow system (Ted Pella) ...
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bioRxiv - Neuroscience 2021Quote: ... A small piece (∼2 × 2 mm) containing V1 was cut out and prepared for EM by staining sequentially with 2% osmium tetroxide (EMS) in Sodium Cacodylate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Biophysics 2024Quote: ... Quantifoil R 2/2 copper 200 mesh grids (Electron Microscopy Sciences #Q2100CR2) were glow-discharged for 45 seconds at 15 mA using a PELCO easiGLOW Glow Discharge System to apply a negative charge to their surface ...
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bioRxiv - Bioengineering 2022Quote: Degassed 2/2-3C C-flat grids (Electron Microscopy Sciences, Hatfield, PA) were glow discharged for 45 s at 30 mA ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS, USA), 0.2% picric acid (EMS ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS 16000), p.H 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... 2% osmium tetroxide (EMS) at at −90℃ for 11hours ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2% glutaraldehyde (EMS) in 0.1 M cacodylate buffer containing 5 mM CaCl2 (pH 7.4) ...
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bioRxiv - Biophysics 2022Quote: C-Flat 2/2-3C or 1.2/1.3-3C grids (Electron Microscopy Sciences) were glow discharged at 10 mA for 90 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... R 2/2 carbon film gold support Quantifoil grids (Q2100AR2; Electron Microscopy Sciences) were loaded into the vacuum chamber and carbon was deposited under high vacuum with stage rotation ...
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bioRxiv - Cell Biology 2022Quote: ... fine-tipped forceps (Dumont #5, EMS). Holding the coverslip vertically ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were then fixed with 2% glutaraldehyde and 2% paraformaldehyde (Electron Microscopy Sciences, #15710) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Developmental Biology 2023Quote: Lungs were fixed in EM fixation buffer (2% glutaraldehyde, 2% paraformaldehyde (Electron Microscopy Sciences), 0.1% calcium chloride in 0.1M sodium cacodylate buffer pH 7.2 ...
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bioRxiv - Neuroscience 2021Quote: ... 2% paraformaldehyde (Electron Microscopy Sciences) in cacodylate buffer (0.15 M sodium cacodylate [Electron Microscopy Sciences] and 0.04%CaCl2 [MilliporeSigma]) ...